The effect of restraint on the content of acid polysaccharides of glandular gastric wall in rat.
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Amino Sugars↗
Biomedical subjects
H Lang
Publications and source records attributed to H Lang.
[The advantages of antecolic gastroenterostomy without Braun's anastomosis in gastric resection according to the Billroth II method].
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Dumping Syndrome↗
[Suitability of the plant protease bromelain for substitution therapy in digestive disorders].
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Bromelains↗
Duplex Doppler sonography of the flaccid penis: potential role in the evaluation of impotence.
PURPOSE: Duplex Doppler sonography of the cavernosal arteries of the penis with intracavernous injection (ICI) of vasoactive agents has been widely used to evaluate arterial insufficiency in impotence. Our goal was to assess the potential value of peak systolic velocity (PSV) measurements on the flaccid penis in the diagnosis of arteriogenic impotence. METHODS: Forty-four men underwent duplex Doppler sonography with PSV measurements before and after ICI of prostaglandin E(1). Three different cutoff values for lowest normal PSV before injection-5 cm/second, 10 cm/second, and 15 cm/second-were tested. RESULTS: Thirteen patients had arteriogenic insufficiency based on post-ICI duplex sonography and clinical response. Results for our different cutoff PSV values of 5 cm/second, 10 cm/second, and 15 cm/second in diagnosing arteriogenic impotence were, respectively: sensitivity 29%, 96%, and 100%; specificity 100%, 92%, and 23%; negative predictive value 80%, 92%, and 100%; positive predictive value 100%, 81%, and 41%; and overall accuracy 79%, 93%, and 44%. In the flaccid state, there was a significant difference in mean PSV between the "normal" group (12.6 +/- 0.9 cm/second) and the arteriogenic impotence group (7.7 +/- 1.1 cm/second). Twenty-nine patients with a bilateral PSV of 10 cm/second or less before ICI had a normal clinical response. CONCLUSIONS: A cutoff PSV value of 10 cm/second in the flaccid state had the best accuracy in predicting arterial insufficiency. Duplex Doppler sonography is proposed as the initial test to evaluate the penile arterial supply and to determine whether patients are good candidates for therapy with ICI.
Adult↗
Role of liver transplantation in management of esophageal variceal hemorrhage.
The management of esophageal variceal hemorrhage ranges from conservative to surgical modalities. Before introduction of liver transplantation as a potentially curative therapy of the underlying etiology, decompressive portosystemic shunt operations have been the mainstay of mostly palliative procedures. Our own experience with surgery for advanced hepatic disease and portal hypertension over 20 years includes 803 liver transplantations and 201 portosystemic shunts, emphasizing our primary objective of treatment. The results after shunt surgery were favorable in Child class A candidates when performed electively and with selective decompression. After liver replacement the clinical status of the patient, including hepatic function and extrahepatic complications, had a strong influence on postoperative outcome, with the chance of excellent long-term survival. The additional risk of previous shunt surgery for subsequent transplantation could be reduced over time. Based on this experience and reports from others there are enough reasonable arguments for shunt and transplantation. Instead of the choice being controversial, the two forms of therapy should supplement each other and be available in the same center that specializes in the treatment of patients with diseases that eventually lead to liver failure and portal hypertension. Selection of either approach must depend on etiology, stage of the disease, and proper timing. Shunt procedures may be indicated in stable patients with the risk of bleeding after sclerotherapy failure, in those with contraindications to transplantation, or as a bridge to transplantation. The role of liver transplantation has been clearly established in patients with progressive or endstage (otherwise intractable) hepatobiliary disease.
Adolescent↗
Role of liver transplantation in the treatment of unresectable liver cancer.
Resection remains the treatment of choice in liver cancer. In order to avoid liver transplantation in conventionally unresectable tumors ex-situ ("bench" procedure), in-situ and ante-situm resection technique should be preferred whenever feasible. Despite the deficiency of donor organs, a single center experience with 198 patients reveals that liver transplantation continues its role as a therapeutic option for selected patients. At present "favorable" indications for transplantation are International Union against Cancer (UICC) - stage II hepatocellular carcinoma as well as the subtype fibrolamellar carcinoma, uncommon tumors such as epitheloid hemangioendothelioma, hepatoblastoma, and liver metastases from neuroendocrine tumors. Due to unsatisfying results, intrahepatic bile duct-, stage III and IV hepatocellular carcinoma, hemangiosarcoma, and liver metastases from nonendocrine primaries should be excluded from liver transplantation alone. For these advanced tumors, especially in cases of extrahepatic involvement, a combination of liver transplantation and multivisceral resection has been proven feasible. However, a significant improvement in patient survival may only be expected only by currently investigated multimodality treatment protocols which will require further randomized studies.
Bile Duct Neoplasms↗
Injection-associated pain in femoral arteriography: a European multicenter study comparing safety, tolerability, and efficacy of iodixanol and iopromide.
PURPOSE: To evaluate injection-associated pain, safety, and efficacy with the isotonic contrast medium iodixanol (Visipaque 270 mg I/ml) compared with iopromide (Ultravist 300 mg I/ml) in femoral arteriography. METHODS: A multicenter, double-blind, randomized, parallel-group clinical investigation was carried out in 54 hospitals in Europe. Of the patients evaluated, 1225 received iodixanol and 1227 iopromide in conventional and/or digital subtraction angiography. RESULTS: The iodixanol group reported statistically significantly less injection-associated pain (0.9%) than the iopromide group (9.5%) (p << 0.001). Further, 4.1% in the iodixanol group experienced pain and/or severe heat sensation vs 19. 8% in the iopromide group (p << 0.001). In the iodixanol group, 1.8% of the patients experienced contrast-related adverse events vs 2.4% in the iopromide group (p = NS). Overall diagnostic information was optimal for 94.1% in the iodixanol group and 95.3% in the iopromide group (p = NS). CONCLUSIONS: Iodixanol 270 mg I/ml causes significantly less injection-associated pain during femoral arteriography and is as safe and efficacious as iopromide 300 mg I/ml.
Aged↗
Rapid, easy and economical dot EIA for detection of antibodies to HIV-1 using recombinant env- and gag-proteins.
A rapid and simple screening test for antibodies to HIV-1 was designed on the principle of dot-EIA. Recombinant HIV-1 env and gag polypeptides are fixed on nitrocellulose sheets. Peroxidase conjugated protein A is used for detection of bound antibodies. After addition of hydrogen peroxide and 2-bromo-1-naphtol antigen-antibody complexes are visualized as discrete blue coloured spots. The test is completed within 15 min. Out of 111 sera positive by commercial EIA and Western blot analysis 110 were recognized by dot-EIA (sensitivity: 99.1%). False positive results compared with commercial EIA were found in 2 of 423 healthy blood donors (specificity: 99.5%).
False Positive Reactions↗
The development of a multicenter database for reference values in clinical neurophysiology--principles and examples.
This paper describes the work undertaken to establish principles for the development of multicenter databases for reference values in clinical neurophysiology. The study was initiated because of interest of the involved laboratories in knowledge-based systems in electromyographic diagnosis, for which it was necessary to formalize the key concepts in the diagnostic process: diseases, pathophysiology and test results. The paper deals specifically with the structuring of results of motor and sensory nerve conduction studies.
Action Potentials↗
Evaluation of column extraction: a new procedure for the analysis of drugs in body fluids.
A method is described for the extraction of drugs from body fluids, whereby liquid-liquid extraction is replaced by liquid-solid elution. The aqueous sample is absorbed on a column filled with dry supporting material. By elution with organic solvents lipophilic substances are extracted from the water phase which remains on the column. The eluate containing the drugs is free of emulsions. For optimal extraction any pH value and a variety of solvents can be used. Column extraction of urine, serum and blood is easily performed and gives high recovery rates, which are superior to conventional extraction. Thin layer chromatography of drugs following conventional XAD-2 and column extraction, demonstrates identical qualitative results, but shows higher purity of the column extracts. The sensitivity of detection is enhanced.
Body Fluids↗
A novel tool for B-cell tolerance research: characterization of mouse alloantibody development using a simple and reliable cellular ELISA technique.
In animal-based transplantation research, the measurement of anti-donor antibodies in transplant recipients is limited by lack of an appropriate technique. We have developed a novel immunoassay capable of quantifying antibody bound to cell-surface major histo- compatability complex (MHC) and non-MHC antigens, using splenocytes from wild-type and MHC-deficient mice as antigen-bearing target cells. We utilized our "cellular ELISA" (CELISA) technique to study the development of tolerance versus immunity in the B-cell compartment in response to neonatal exposure to allogeneic fetal liver cells (FLC). This neonatal tolerance protocol typically induces permanent acceptance of donor-type and third-party cardiac allografts, but rejection of both donor-type and third-party skin grafts occurs. C3H/He (C3H; H-2(k)) mice were injected as neonates with BALB/c (BALB; H-2(d)) FLC and transplanted as adults with C57BL/6 (B6; H-2(b)) cardiac grafts. Despite long-term acceptance of third-party B6 cardiac grafts, serum contained increased anti-B6 IgG and IgM levels as measured by CELISA; IgM production was elevated by 2 weeks posttransplant and remained stable, while IgG production increased rapidly between 2 and 5 weeks posttransplant. In another experimental setting, CELISA assays were able to detect that neonatal injection of C3H mice with FLC from wild-type B6 mice or from MHC class II-deficient or class I/II-deficient (B6 background) mice (CI(+)CII(+), CI(+)CII(-), CI(-)CII(-), respectively) prevented sensitization to B6 antigens by subsequent skin transplants but did not induce graft acceptance, whereas FLC from MHC class I-deficient-only (CI(-)CII(+)) did not prevent B6 sensitization. The CELISA technique is a simple and sensitive means for quantifying alloantibodies in mice and will assist in further delineating the role of the B-cell compartment in neonatally induced cardiac allograft acceptance.
Animals↗
Classifications in routine use: lessons from ICD-9 and ICPM in surgical practice.
OBJECTIVE: Classifications of diagnoses and procedures are very important for the economical as well as the quality assessment of surgical departments. They should reflect the morbidity of the patients treated and the work done. The authors investigated the fulfillment of these requirements by ICD-9 (International Classification of Diseases: 9th Revision) and OPS-301, a German adaptation of the ICPM (International Classification of Procedures in Medicine), in clinical practice. DESIGN: A retrospective study was conducted using the data warehouse of the Surgical Center II at the Medical Faculty in Essen, Germany. The sample included 28,293 operations from the departments of general surgery, neurosurgery, and trauma surgery. Distribution of cases per ICD-9 and OPS-301 codes, aggregation through the digits of the codes, and concordance between the classifications were used as measurements. Median and range were calculated as distribution parameters. The concentration of cases per code was graphed using Lorenz curves. The most frequent codes of diagnoses were compared with the most frequent codes of surgical procedures concerning their medical information. RESULTS: The total number of codes used from ICD-9 and OPS-301 went up to 14 percent, depending on the surgical field. The median number of cases per code was between 2 and 4. The concentration of codes was enormous: 10 percent of the codes were used for about 70 percent of the surgical procedures. The distribution after an aggregation by digit was better with OPS-301 than with ICD-9. The views with OPS-301 and ICD-9 were quite different. CONCLUSION: Statistics based on ICD-9 or OPS-301 will not properly reflect the morbidity in different surgical departments. Neither classification adequately represents the work done by surgical staff. This is because of an uneven granularity in the classifications. The results demand a replacement of the ICD-9 by an improved terminological system in surgery. The OPS-301 should be maintained and can be used at least in the medium term.
Disease↗
Experimental investigation of intraoperative ultrasound in anatomically precise liver resection.
A new technique of segmental liver resection by use of intraoperative ultrasound (IOUS) and injection of methylen blue was evaluated in 15 sheep to study anatomical precision and alterations of the biochemical profile. The results were compared with those of a bisegmentectomy (n = 15) in which segmental boundaries were identified by IOUS alone. In a third group (n = 10) a sham operation (laparotomy without resection) was performed to study the intrahepatic vascular architecture of the liver by IOUS. The quality of the resections and of the ultrasound study was assessed by use of corrosion casts of the livers. The intrahepatic course of the liver veins and their confluence as well as the portal vein and its branches up to the segmental portal pedicles could be detected in all livers. Anatomically precise bisegmentectomies were achieved in 70% of IOUS combined with coloring of the segments versus only 27% in IOUS alone (p < .05). Biochemical and clinical parameters did not reveal any advantage of anatomically precise resection. The results show that intraoperative ultrasound is reliable in visualizing the intrahepatic anatomical characteristics of the liver, but it is imprecise in identifying segmental boundaries or in localizing a segment exactly. Anatomically precise liver resections are technically feasible by use of IOUS plus selective staining of the segments.
Animals↗
High-dose mitomycin C in isolated hyperthermic liver perfusion for unresectable liver metastases.
In order to reduce systemic side effects and increase intrahepatic mitomycin C (MMC) concentrations, isolated hyperthermic liver perfusion (IHLP) has been performed using MMC. This article describes the pharmacokinetics of MMC in IHLP and presents our clinical experience with its use in six patients suffering from unresectable liver metastases. Primary tumors consisted of colorectal carcinomas in three cases, breast cancer in two, and a choroidal melanoma in one. Dosages of MMC varied between 0.5 and 1.0 mg MMC/kg body weight. MMC was added as a bolus directly into the extracorporeal circuit. Intrahepatic temperature was elevated to 40.0-41.0 degrees C by hyperthermic perfusion. MMC concentrations were measured in peripheral blood (preperfusion, then at 5, 30, and 55 min during perfusion, and finally at 5 and 60 min and 6 and 24 h after perfusion) and in recirculating perfusate (5, 30, and 55 min). While markedly elevated MMC concentrations (maximum 6290 ng/mL) were found in the liver perfusate, systemic concentrations remained low (maximum 45 ng/mL), indicating no considerable leakage. MMC concentrations in the perfusate constantly decreased during perfusion. After rinsing with 1500 mL saline, a mean concentration of 52.5+/-33 ng MMC/mL was measured in the washout from 5 patients. In 1 patient with a colorectal carcinoma, MMC concentrations in the perfusion medium were 10-fold and in the plasma 2-fold higher than in the other patients. This high MMC concentration caused severe intrahepatic vascular damage and finally led to the patient's death. In conclusion, IHLP and intrahepatic perfusion with MMC resulted in a high response of hepatic tumors. Systemic exposure of MMC can be reduced effectively by isolated perfusion. However, hepatic toxicity of MMC must be considered.
Adult↗
A porcine model for investigation of hyperthermic isolated liver perfusion.
Our study was aimed at developing a reliable method of hyperthermic isolated liver perfusion (HILP) in pigs and at assessing its local and systemic side effects. HILP was performed via the hepatic artery and portal vein in 15 animals. The perfusate consisted of blood (200 ml), oxypolygelatine (500 ml), Ringer's solution (1000 ml), and trapped intrahepatic blood. HILP was carried out for 45 min at a mean perfusate inflow temperature of 41.2 degrees C. The mean portal flow and pressure were adjusted to 500 ml/min and 20-25 mm Hg; the mean arterial flow and pressure were 130 ml/min and 40-60 mm Hg, respectively. After 20 min of perfusion the mean temperature in the right and the left liver lobe were 40.8 degrees C and 40.3 degrees C and remained almost constant over the whole perfusion period. Liver enzymes (alanine aminotransferase and aspartate aminotransferase) and serum lactate levels showed slight increases after perfusion but normalized within 1 week. Histology of liver parenchyma showed only mild pathological changes, which were also reversible within 7 days. The presented method of HILP is a safe and reproducible technique for isolated hyperthermic liver perfusion. Based on this animal model, experimental HILP with different chemotherapeutic agents can be investigated in order to assess hepatic and systemic toxicity of this therapy.
Animals↗
An in vitro comparison of metal and transparent matrices used for bonded class II resin composite restorations.
This study compared excess formation of direct bonded Class II restorations using different matrix systems-metal or transparent. Sixty freshly extracted, non-carious, posterior human teeth were used. In all of the teeth, standardized MOD-cavities were prepared with the gingivoproximal margins located 1.0-1.5 mm cervical to the cemento-enamel junction. The prepared teeth were randomly assigned to six groups. Half were restored using metal matrices and wooden wedges; the other half were restored using transparent matrices and reflective wedges. Three different material systems were used to fill the cavities: 1) a hybrid composite (Tetric) plus an adhesive bonding agent (Syntac Classic), 2) a flowable composite (Tetric Flow) plus Syntac Classic and 3) a compomer (Dyract AP) together with an adhesive bonding agent designed for compomers (Prime & Bond NT). After the specimens were preserved in saline solution, scanning electron microscopy (SEM) assessed the amount of overhang formation at the restoration margins. The data collected indicated the use of transparent matrices resulted in significantly higher amounts of excess material at the restoration margins compared with metal matrices. Moreover, there was no significant difference between the materials when the same matrix was used. All of the dental restorations examined displayed material overhang. Based on these findings, the authors concluded that the type of matrix exerts a major impact on overhang formation, with metal matrices resulting in significantly less excess material buildup.
Compomers↗
Increment technique for extended Class V restorations: an experimental study.
The application and polymerization of composite or polyacid-modified resin composites in thin layers (increment technique) for filling cavities might partially compensate for the stress associated with polymerization shrinkage. In this study, the effect of this technique on the marginal integrity of Class V polyacid-modified composite restorations was investigated. In 30 extracted premolars, extended Class V cavities were prepared with the apical margin in root dentin and the coronal margin in enamel. They were then subjected to different treatments (10 teeth in each case): a) placement and polymerization of a thin resin composite layer (Dyract-Dyract PSA) in the coronal part of the cavity (plus one increment for the residual part) (Group 1), b) a thin layer at the apical part of the cavity (plus one increment for the residual part) (Group 2) and c) restoration of the entire cavity with one increment (bulk technique) (Group 3). Following three days' storage in water, the teeth were subjected to cyclic thermal loading (4 degrees C <-> 55 degrees C, 2,000 cycles). The percentages of defective bonding detected along the total length of the restoration margin were assessed before and after thermocycling by scanning electron microscopy. Following loading and thermocycling, no significant differences were found at the restoration-enamel interface. Fewer gaps were found at the restoration-dentin interface in Group 1 (18.7 +/- 25.7%) and Group 2 (20.5 +/- 22.8%) compared to the reference Group 3 (42.2 +/- 30.6%) (Mann-Whitney U test: p<0.05). Moreover, there were no significant differences between the two increment groups (1 and 2). In a significantly larger number of cases, a completely intact dentin restoration margin was detected when a coronal increment (Group 1) (44.4%) was used instead of the bulk technique (Group 3) (13.6%) (Chi square test: p<0.05). These results indicate that gap formation can be significantly minimized by using an increment technique to restore extended Class V cavities with polyacid-modified composite materials.
Chi-Square Distribution↗
[Coping with illness in bronchial cancer from the viewpoint of the patients, their relatives and caregivers. A five-level assessment].
The investigation presented in this paper applies multi-level-assessment to emotional state and coping in patients with newly diagnosed bronchogenic carcinoma. Ratings are obtained by the patients themselves, their spouses/children, their physicians, their nurses, and interviewers, independently. Differences seem to override concordances. Patients generally present themselves in a more positive way, i. e. emotionally less disturbed, better adapted, and more successfully coping, than generally perceived by their associates. Discrepancies regarding specific aspects of illness recognition and coping give information about conflicts in both doctor-patient-relationship (e. g. greater amount of trust from the patients' than from the doctors' point of view) and nurse-patient-relationship (nurses claim patients being more aggressive then patients see themselves), as well as social support. Patients and relatives agree in their ratings of certain criteria of adaptation (getting along with the disease, quality of life) and in effective treatment possibilities. On the basis of factor analyses which are computed separately for each rating level, the perceptual structures of the different raters can be elaborated on.
Adaptation, Psychological↗