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H Kushiro

Publications and source records attributed to H Kushiro.

At least 37 records · Page 2Linked to original sources
PubMed1980

Comparison of three blood-clotting substances in Staphylococcus aureus strains.

Besides the two well-known blood-clotting substances, coagulase and clumping factor, a third one has been identified from the staphylococci which is a cell surface polysaccharide, is alkali stable, and induces compact-colony formation in serum soft agar. Using some 97 clinical strains of Staphylococcus aureus, we found that in production and activity the substances were distinctly different.

Agar↗
PubMed1975

[A study on the determination method of cyclic AMP in urine by competitive protein binding assay (author's transl)].

The determination method of c-AMP in urine by competitive protein binding assay using the Boehringer Mannheim Laboratory kit was investigated and the results obtained were as follows. 1) Since the angle of the inclination of the standard curve was large, the present method could be used for the determination of c-AMP in concentrations of from 0 to 20p moles. 2) The optimal condition of the binding reaction was for 100 minutes of reaction time at pH 4.0 and 4 degrees C. 3) The specificities of binding protein to the other nucleotides were 0 to adenosine, 0.4 to AMP, 0.3 to ADP, 0.4 to ATP and 0.6 to c-GMP respectively when the specificity to c-AMP was chosen as 100. 4) The optimal volume of cold phosphate buffer solution needed for washing to separate binding c-AMP by filtration method using a millipore filter was 5 approximately 15 ml. 5) The precision of the present method by double determination was 0 arrroximately +/- 11.1% with average of +/-5.6% in c.v. 6) The recovery rate of the added c-AMP by the present method was 78.6 approximately 105.6% with average of 90.1%. 7) Correlation between the determination values of c-AMP of the same samples with the present method and radioimmunosasay (Schwarzman Laboratory Kit) was satisfactorily high with 0.890 in coefficient of correlation, and the determination values by the present method were significantly higher (p less than 0.05) than those by radioimmunoassay. 8) C-AMP in urine was stable for at least one month when the urine was kept frozen. It is conclusively considered from the above results that this competitive protein binding assay is a method to determine c-AMP in urine with excellent accuracy and sensitivity and that this method is useful for clinical test.

Binding, Competitive↗
PubMed1972

[Total lipids].

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Cholesterol↗