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Biomedical subjects

H Kubo

Publications and source records attributed to H Kubo.

At least 253 records · Page 14Linked to original sources
PubMed1992

[A case of tracheobronchopathia osteoplastica discovered after difficulty in intubation].

A 77-year-old man was scheduled for Love's method to correct lumbar disc herniation. He had fracture of maxilla and mandibula during World War II. Physical examination and laboratory data revealed no abnormalities. Anesthesia was induced with oxygen, nitrous oxide and halothane. Following administration of succinylcholine, an attempt was made to insert a tracheal tube (from 8.5mm to 5.5mm), but the tubes were met with an obstruction. The operation was cancelled because tumor-like nodules were seen in the trachea. Diagnosis of tracheobronchopathia osteoplastica was made afterwards following biopsy. The patient with this disease has a good prognosis. Artificial ventilation can be performed safely by choosing an appropriate method for airway maintenance.

Aged↗
PubMed1992

Exercise training and the prevention of restenosis after percutaneous transluminal coronary angioplasty (PTCA).

To evaluate whether exercise training prevents the restenosis after percutaneous transluminal coronary angioplasty (PTCA), treadmill exercise with thallium-201 myocardial scintigraphy was performed at first and thirteenth week after PTCA in 18 patients with training and in 20 patients without training. Total treadmill exercise duration and maximal pressure rate product 13 weeks after PTCA increased significantly in the trained group, whereas there was no significant change in the untrained group. ST segment depression from the baseline did not change significantly in both groups. Myocardial perfusion obtained by Tl-201 scintigraphy improved significantly in the trained group, whereas there was no significant change in the untrained group. The restenosis rates at the third month after PTCA was 17% (3/18) in the trained group and 40% (8/20) in the untrained group. It is concluded that in patients with coronary artery disease, exercise training improves myocardial perfusion by preventing the progression of coronary artery stenosis after PTCA.

Angioplasty, Balloon, Coronary↗
PubMed1992

[Analysis of cell proliferation kinetics and the effects of cisplatin on the cell cycle of human gastric cancer cells by autostage cytofluorometry].

Analysis of both cell proliferation kinetics and effects of cis-diamminedichloroplatinum (CDDP) on cell cycle in human gastric cancer cell line (HGC-Y2) by measuring the contents of nuclear DNA, RNA and the Ki-67 antigen using autostage cytofluorometry system was described. In HGC-Y2 cells, RNA content increased during the cell cycle and reached to the maximum at G2/M phase. The results of pulse treatment with CDDP on these cells demonstrated a prolongation of S phase and G2 arrest with increasing of RNA content of these cells. We classified the cells by intranuclear distribution pattern of Ki-67 antigen and thus could identified the cells at G0 and M phases from these classification. The content of Ki-67 antigen was moderate grade at G1 phase and it decreased in the early S phase, then increased gradually during S phase and at the late S phase. It increased rapidly, reaching to the maximum at G2/M phase. After CDDP treatment, the content of Ki-67 antigen increased in the cells in prolonged S phase and in the cells arrested at G2 phase. It was also found that the syntheses of both Ki-67 antigen and RNA were not inhibited by CDDP. These results suggest that the method using autostage cytofluorometry system was useful for the research, on the mechanism of cancer therapy because of making possible to analyze precisely the cell cycle and the influence of anticancer drugs.

Cell Cycle↗
PubMed1991

Developmental change in ganglioside expression in primary culture of rat neurons.

Developmental changes in ganglioside levels and patterns were investigated in neuronal cells dissociated from 17-day-old fetal rat hemispheres for up to 7 days of culture. Increases in ganglioside contents and the onset of GM3 synthesis, which is associated with proliferation of glial cells, were observed as the neuronal network was established in cell cultures. The distribution of gangliosides in developing neurons was monitored by the indirect immunofluorescent technique using three anti-ganglioside antibodies. Anti-GM1 antibody showed immunofluorescence only on the cell soma 1 and 3 days after plating and additional binding between cell aggregates by 7 days in culture. GD3 ganglioside, the predominant species in embryonic neurons, was not detected on the neuronal cell surface, whereas the number of positively stained non-neuronal cells was increased at 7 days. Monoclonal A2B5 antibody suggested that polysialogangliosides play a role in neuronal network formation. In 1-day-old culture, however, all antibodies bound poorly to cell surface antigens and strongly to cells, the membranes of which were permeabilized with acetone. These results suggest that a substantial amount of gangliosides are retained, transformed within the cell to more complex gangliosides, and translocated to the cell surface following neurite outgrowth and morphological changes.

Animals↗
PubMed1991

Bestatin administration and the change in cellular immunity.

The change in cellular immunity by Bestatin (ubenimex) treatment--30 mg/day orally--was investigated in 23 gastrointestinal cancer patients for: 1), functional T cell subsets; 2), IL-2 receptor; 3), PHA-induced blastogenesis; and 4), PPD skin reaction. The absolute number of helper T cells (Th) and cytotoxic T cells (Tc) increased in 74 and 79% of cases, respectively, compared with pretreatment values. On the other hand, the absolute number of suppressor T cells (Ts) decreased in 79% of cases. IL-2 receptor increased in 56% of patients, PHA blastogenesis increased in 67% of patients and PPD skin reaction was elevated in 75% of cases comparing to pretreatment values. These results suggest that Bestatin could increase cellular immunity in cancer patients.

Antibiotics, Antineoplastic↗
PubMed1991

Fluorometric determination of isoniazid and its metabolites in urine by high-performance liquid chromatography using in-line derivatization.

A rapid, simple, and accurate method has been developed for the determination of isoniazid and its metabolites (isonicotinic acid, isonicotinylglycine, and acetylisoniazid) in human urine by high-performance liquid chromatography. Isoniazid and its metabolites are separated by reversed-phase ion-exchange chromatography with a mobile phase containing hydrogen peroxide as a fluorogenic reagent and butanesulfonate as a hydrophobic ion exchanger, and are detected by fluorometry (excitation at 317 nm and emission at 415 nm) using in-line derivatization at high temperature (160 degrees C). The detection limits are isonicotinic acid, 0.5 mumol/L; isonicotinylglycine, 1 mumol/L; acetylisoniazid, 1 mumol/L; and isoniazid, 1.5 mumol/L. This method can be applied for acetylator phenotyping.

Chromatography, High Pressure Liquid↗
PubMed1991

Effect of reticuloendothelial blockade on tissue distribution of 99mTc-labeled synthetic liposomes in Ehrlich solid tumor-bearing mice.

The effect of a reticuloendothelial blockade was examined on the tissue distribution of 99mTc-labeled synthetic liposomes prepared from N,N-didodecyl-N alpha-[6-(trimethylammonio)hexanoyl]-L-alaninamide bromide (N+C5Ala2C12) in Ehrlich solid tumor-bearing mice. While a pre-dose of unlabeled phosphatidyl choline liposomes (natural liposomes) hardly influenced the tissue distribution of N+C5Ala2C12 liposomes, the pretreatment of dextran sulfate depressed the uptake in liver accompanied by increasing that in tumor and other tissues except the stomach. However, the extent of liver depression of N+C5Ala2C12 liposomes by dextran sulfate was lower than that of natural liposomes and the pre-dose of unlabeled natural liposomes had a minor effect on the tissue distribution of N+C5Ala2C12 liposomes compared with that of natural liposomes. In the liver uptake of N+C5Ala2C12 liposomes, it was suggested that Kupffer cell phagocytosis was not the main mechanism.

Animals↗
PubMed1991

[Decreased cerebral blood flow in remote areas in deep-seated cerebrovascular lesions].

Recent positron or single photon emission computed tomographic studies have demonstrated that the impairment of regional hemodynamics or metabolism in cerebrovascular disease is not limited to the focal lesion, but also involves more remote areas. The present study evaluated cerebral blood flow in areas remote from the primary lesion in patients with subcortical cerebrovascular disease, and assessed correlations with the clinical features. Twenty patients aged 61-89 (mean, 74.7 +/- 6.7 years) were divided into the L group (lacunar infarction in deep penetrating arterial territory, n = 7), the NL group (non-lacunar subcortical infarction caused by main trunk occlusion or severe stenosis, n = 6), and the H group (putaminal or thalamic hemorrhage, n = 7). Ipsilateral cortical and contralateral cerebellar blood flow were assessed using single photon emission computed tomography with 123I-IMP. Although only some patients of the L group showed a mild reduction of ipsilateral cortical blood flow, most of the patients in the NL and H groups showed a moderate or severe flow reduction. The cortical blood flow reduction was suggested to be caused by several mechanisms, including brain edema, a decreased perfusion pressure associated with disease of large arteries, partial cortical neuronal damage, and deactivation. The degree of the flow reduction was considered to depend on the relative contribution of each of these factors. Contralateral cerebellar blood flow reduction, i.e., crossed cerebellar diaschisis, was observed more frequently and prominently in the NL and H groups than in the L group.(ABSTRACT TRUNCATED AT 250 WORDS)

Aged↗
PubMed1991

Prenatal diagnosis of Duchenne muscular dystrophy by restriction fragment length polymorphism analysis with pERT 87 intragenomic deoxyribonucleic acid probes.

Prenatal diagnosis of DMD was performed with three intragenic genomic probes and chorionic villus sampling. A total of 8 unrelated families with at least one DMD were analysed. DNA was extracted from peripheral white blood cells for carrier testings (50 individuals). For prenatal detection, it was extracted from chorionic villi obtained by chorionic villus sampling at 9 menstrual weeks. DNA was digested with an appropriate restriction enzyme followed by overnight electrophoresis in 1% agarose gels. DNA was transferred from the gel to nylon membrane according to the protocol of an alkaline transfer method. The pERT 87 probes were labeled by nick translation. The membranes were hybridized overnight after prehybridization. After washing, the membranes were exposed to X-ray films to make autoradiograms for restriction fragment length polymorphism analysis. Fetal sex was determined by a rapid screening test with a Y chromosome-specific repeat sequence. Out of 8 fetuses, 4 were males and 4 were females. All of 4 male fetuses were determined to be unaffected. Out of 4 female fetuses, 3 were diagnosed as non-carriers, and the carrier status of the remaining one was not able to be determined because her mother was not informative for all testings.

Chorionic Villi Sampling↗
PubMed1991

[Quantitative determination of furosemide in serum and urine by high-performance liquid chromatography with electrochemical detector].

A high-performance liquid chromatographic method using electrochemical detection is described for the determination of furosemide in serum and urine. Serum proteins were precipitated with acetonitrile containing the internal standard and the clear supernatant was separated. Urine was diluted with water to 50 times. Furosemide was chromatographed on a reversed-phase column, 8MBC18 column, using 35% ethanol solution containing 5 mmol/L tetrabutylammonium phosphate (pH 7.50) as the mobile phase (1.0 ml/min) and detected at 0.90 volts. FD-Val-OH synthesized was used as internal standard in the furosemide assay. The retention times for furosemide and internal standard were 10 min and 15 min, respectively. The amount of furosemide was determined by measuring peak height ratio. The detection limit of furosemide was 16 and 9 ng per ml serum and urine respectively. The calibration curve was linear in the range from 0.25 ng/microliters to 5 ng/microliters (serum) and from 0.5 ng/microliters to 10 ng/microliters (urine). The recovery of furosemide was 100.5% (serum) and 100.6% (urine). Coefficient of variation was less than 4.6%.

Chromatography, High Pressure Liquid↗
PubMed1991

[Fluorometric determination of pyridine and its derivatives by flow injection analysis].

A method for the fluorometric determination of pyridine and its derivatives has been developed by flow injection analysis using hydrogen peroxide at high temperature. The reaction system consists of two pumps to deliver reagent and carrier stream, sample injector, reaction coil (0.5 mm ID x 15 m, 150 degrees C), cooling coil (0.5 mm ID x 3 m, 30 degrees C), and cooling coil (0.5 mm ID x 20 cm, 0 degrees C). The wavelengths of the fluorometric spectrophotometer were set at Ex 305-350 nm and Em 380-410 nm, the flow rate of each solution was 1.0 ml/min. The carrier stream was deionized water. The reaction solution containing 10 mmol/L hydrogen peroxide in 0.2 mol/L phosphate buffer (pH 6.0) gave the maximum fluorescence intensity for pyridine and its derivatives. Linear calibration curves were obtained from 5 ng up to 100 ng of pyridine and its derivatives. The coefficient of variations for 2.5 ng (n = 10) and 25 ng (n = 10) of isonicotinic acid, isoniazide and acetylisoniazide were 1.8% and 1.1%, 1.6% and 1.2%, 2.1% and 1.4%, respectively. The detection limit (S/N = 3) was 250 pg for isonicotinic acid, 500 pg for isoniazide, acetylisoniazide, nicotinamide, isonicotinamide, nicotinic acid, and 2.5 ng for pyridine, nicotine, 2--picoline, 2--picolinamide and picolinic acid. The carrier stream containing organic solvent (methanol, ethanol or acetonitrile) decreased the fluorescence intensity, but in the case of acetonitrile there was less decrease than methanol or ethanol. This method allowed the analysis of 30 samples/h.

Flow Injection Analysis↗
PubMed1991

[YAG laser contact therapy of ovarian endometriosis under laparoscopy].

Thirty-six patients with ovarian endometriosis were treated with Nd:YAG laser contact irradiation under laparoscopic control. Indications for laparoscopy were infertility (n = 20) and dysmenorrhea (n = 16). The laparoscopic procedures so far undertaken in our clinic include: Aspiration of chocolate cyst, removal of ovarian endometriosis, adhesion-lysis, uterine nerve ablation, coagulation of peritoneal endometriosis and irrigation. No complications were seen. After undergoing this procedure, eight of 20 patients achieved pregnancy and 15 of 16 patients obtained pain relief. Serum CA125 levels were significantly decreased postoperatively. We confirmed that contact irradiation with a cone-shaped sapphire probe provides adequate incision and lysis at lower power levels, and that this method is an effective treatment for ovarian endometriosis.

Adult↗
PubMed1991

[Coronary collateral vessels during the early period of acute myocardial infarction: their development].

The aim of this study was to investigate the incidence and development of coronary collateral circulations in patients with acute myocardial infarction (AMI). We categorized 165 patients with persistent 100% occlusion of the infarct-related artery into 6 groups according to the time from the onset of AMI to angiography. Group I consisted of 55 patients evaluated within 6 hours after the onset of AMI; Group II, 28 patients, between 6 and 12 hours after the onset; Group III, 12 patients, between 12 and 24 hours after the onset; Group IV, 11 patients, between 2 and 13 days after infarction; Group V, 46 patients, between 14 and 44 days after infarction; and Group VI, 13 patients, more than 45 days after infarction. Collateral vessels were applied a numerical score between 0 and 3 according to the degree of opacification of the native vessel distal to the occlusion. In 58%, 79%, 67%, 73%, 89%, and 92%, patients of Groups I to VI had evidence of collateral vessels, respectively. Well-developed collaterals were observed in 24% of Group I compared with 50%, 58%, 55%, 73% and 69% of patients in Groups II to VI, respectively. The mean coronary collateral scores were 0.9 +/- 0.1, 1.4 +/- 0.2, 1.4 +/- 0.3, 1.6 +/- 0.4, 2.0 +/- 0.2 and 2.2 +/- 0.3 for Groups I to VI, respectively. Patients with preinfarction angina had more well-developed collateral circulations than did patients without it, however, there was no significant correlation between the duration of previous angina and extent of coronary collaterals.

Adult↗