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Biomedical subjects

H Komatsu

Publications and source records attributed to H Komatsu.

At least 199 records · Page 11Linked to original sources
PubMed1996

[A clinical and pathological study of radical prostatectomy for prostate cancer].

BACKGROUND: Thirty-one patients with prostate cancer underwent radical prostatectomy and simultaneous pelvic lymphadenectomy at Matsumoto National Hospital between 1988 and 1994. Prognostic factors are discussed from their clincopathological findings. METHODS: The patients ranged from 54 to 80-year-old, with an average age of 69.9 years. The median follow-up period was 44 months. The diagnosis was confirmed by needle biopsy or transurethral resection of the prostate. All the patients received short-term endocrine therapy preoperatively, and only noncuratively resected patients underwent adjuvant therapy postoperatively. At initial diagnosis, the tumor grades were well, moderately, and poorly differentiated adenocarcinoma in 9, 12, and 10 patients, respectively. The clinical stage was defined as A2, B, C, D1, and D2 in 12, 4, 6, 3, and 6 patients, respectively. RESULTS: A difference of tumor grade was found between the initial diagnosis and the final diagnosis based on the resected prostate in 8 patients (26%), with 7 of them (88%) showing an increase in grade in the final diagnosis. Also revealed was that 11 of the 25 patients (44%) in stage A2, B, C, or D1 had been understaged preoperatively. The five-year actuarial survival rates were 100%, 92%, and 51% for patients with well, moderately, and poorly differentiated adenocarcinoma, respectively, with a significant difference noted between well and poorly differentiated adenocarcinoma (p = 0.03). Recurrence only developed in patients with pathological stage D tumors. However, the presence or absence of lymph node metastasis did not affect the crude 5-year survival rate. Several stage D patients were successfully treated by radical prostatectomy and adjuvant therapy, achieving long survival. CONCLUSION: These results indicate that patients in clinical stage C have tumors which exhibit differing biological behavior. These patients should be analyzed and classified more precisely so that the most appropriate therapy can be chosen.

Adenocarcinoma↗
PubMed1996

Viral RNA binding properties of human immunodeficiency virus type-2 (HIV-2) nucleocapsid protein-derived synthetic peptides.

The nucleocapsid (NC) protein of HIV-2 (NCp8) contains two Cys-His arrays which function as zinc finger motifs (ZFMs). In this study, we analyzed the viral RNA-binding properties of NCp8-derived synthetic peptides using ultraviolet (UV) cross-linking assay. Several synthetic peptides containing ZFM(s) interacted pH-dependently with in vitro-synthesized HIV-2 RNA. Although the peptides corresponding to the 1st and 2nd ZFMs, respectively, failed to interact with the viral RNA, the corresponding peptides flanked by basic amino acid clusters interacted tightly. Furthermore, basic amino acid residues within a cluster adjacent to ZFMs contributed to the RNA-binding of NCp8 more than Cys and His residues within the ZFM in vitro. In competitive UV cross-linking assay using non-specific RNA as a competitor, the peptides corresponding to the 1st and 2nd ZFMs flanked by basic amino acid clusters interacted specifically with viral RNA. These findings suggest that both ZFM regions of HIV-2 may be concerned with the specificity of packaging of genomic viral RNA into the virion.

Amino Acid Sequence↗
PubMed1996

[The effect of continuous epidural infusion of combination of buprenorphine and bupivacaine for postoperative pain relief using a portable 0.5 ml.h-1 type infuser with patient control module].

Using a portable 0.5 ml.h-1 type infuser with Patient Control Module (Baxter infuser BB+ PCM), we compared patients receiving continuous epidural infusion with patients using self controlled analgesia system for postoperative analgesia after upper abdominal surgery. Twenty-one patients were randomized into three groups: group I (n = 7) received 20 micrograms.h-1 of buprenorphine (Bu) with additional 20 micrograms of Bu; group II (n = 7) 20 micrograms.h-1 of Bu plus 1 mg.h-1 of bupivacaine (Bup) with additional 20 micrograms of Bu plus 1 mg of Bup; group III (n = 7) 20 micrograms.h-1 of Bu plus 2 mg.h-1 of Bup with additional 20 micrograms of Bu plus 2 mg of Bup. In all three groups, patients received supplemental Bu 0.1 mg intramuscularly as needed. During 48-hours postoperatively, we evaluated verbal descriptor pain scale, sedative scale, visual analogue scale, supplemental doses of Bu, and side effects. Total doses of Bu during the first 12 hours were significantly larger than those during other 12-hour period in all the groups (P < 0.05). In each period during the 12 to 48-hours after operation, the percentage of the patients who needed no supplemental Bu was 71.4-100%, which is higher than during the 0 to 12-hours (47.7%). There was no significant difference in verbal descriptor pain scale, sedative scale, visual analogue scale, and the incidence of side-effect among the three groups. Continuous epidural infusion with Bu using a portable 0.5 ml.h-1 type infuser with patient control module was effective for alleviating postoperative pain except in the first 12-hour period. However, addition of Bup to Bu did not improve the quality of postoperative epidural analgesia.

Aged↗
PubMed1996

[An elderly patient with traumatic acute subdural hematoma who "talked and deteriorated"].

A 62-year-old man was transferred to our hospital 20 min after a motor vehicle accident. The CT scan on admission revealed mixed density area of acute subdural hematoma, but he was asymptomatic on physical examination. Course observation without brain edema therapy resulted in severe disturbance of consciousness 2 hours after the traffic accident. The CT scan at this time showed the enlargement of the hematoma, and midline shift. Emergent external decompression was performed 4 hours after the head trauma, but he died of heart failure on the 8 th day after the operation. We consider that the important factors for saving this patient were rapid diagnosis by repeated CT scan and conservative brain edema therapy early in the post-traumatic period.

Accidents, Traffic↗
PubMed1996

[Changes of urinary ulinastatin and serum CRP after elective surgery for gastric cancer].

Urinary ulinastatin (UTI) is elevated in various conditions, such as renal disease, myocardial infarction, neoplasm, leukemia and normal pregnancy. The purpose of the present study is to measure the change of UTI after surgery for gastric cancer and to compare with other acute phase reactants. Urine samples were collected from 7 surgical patients. UTI level was measured by radioimmunoassay and corrected by urinary level of creatinine. UTI levels began to increase from the first postoperative day and reached the maximum level on the third postoperative day. The pattern of UTI correlated with that of serum CRP levels. Meanwhile, when patient was in inflammatory condition postoperatively, UTI increased further and its pattern correlated with that of serum CRP levels. These results suggest that UTI is an acute phase reactant and a sensitive marker indicating the degree of inflammatory condition after surgery.

Acute-Phase Proteins↗
PubMed1996

[Airway management in complete transection of the trachea due to traffic accident].

Oro-tracheal intubation to prevent the development of subcutaneous emphysema after blunt trauma by a traffic accident resulted in progressively worsening dyspnea because of complete transection of the trachea. Emergent tracheal reconstruction was required for life-saving. In order to secure a safe and effective airway in such a patient, we should diagnose accurately and select the methods of securing the airway earlier in the post-traumatic period.

Accidents, Traffic↗
PubMed1996

[Myelodysplastic syndrome developed in a mother and her son whose bone marrow karyotype showed monosomy 7].

Two familial cases of myelodysplastic syndrome (MDS) are reported, one of whom had an abnormal karyotype of 45, XY, -7 (monosomy 7). Case 1 was a 60-year-old woman developed dizziness and nasal bleeding. She was treated with blood transfusion alone. About 11 months after diagnosis, she died of pneumonia. Case 2 was a 22 year-old man, who was the son of case 1, developed febrile disease because of recurrent skin and oral mucosa infections. He had a partial response to low-dose of cytarabine. Thirteen months after diagnosis, he died of severe pneumonia. Both cases were diagnosed as having refractory anemia with excess of blasts due to peripheral blood and bone marrow findings. Both patients had pancytopenia, erythroid hyperplasia in bone marrow, marked dyserythropoiesis, recurrent infectious diseases and severe pneumonia that resulted in death. These symptoms resembled to those reported for monosomy 7 syndrome. Familial MDS with monosomy 7 is rarely reported. These cases are of interest to investigate hereditary factors of MDS.

Adult↗
PubMed1996

[Postoperative urinary ulinastatin secretion and renal function in hepatectomized patients with and without liver cirrhosis].

We compared ulinastatin secretion into urine with renal function during postoperative period in three groups, hepatectomized group with liver cirrhosis (LC(+), n = 7), hepatectomized group without liver cirrhosis (LC(-), n = 4) and subtotal gastrectomized group with normal liver function (GR, n = 7). In LC(+) group, N-acetyl-beta-D-glucosaminidase (NAG) increased above normal upper limit throughout the postoperative period. Ulinastatin (UTI) also increased but the increase was not remarkable. In LC(-) group, NAG increase but not significantly compared with the preoperative value. UTI increased from 5.8 +/- 3.0 IU.mg x Cr-1 to 30.8 +/- 16.6 IU.mg x Cr-1 and 39.9 +/- 9.0 IU. mg x Cr-1 on the 1st and 3rd postoperative day respectively. In GR group, there was no change in NAG value. UTI on the 3rd postoperative day increased significantly (from 10.0 +/- 7.7 to 75.4 +/- 39.0 IU.mg x Cr-1). A small urinary secretion of UTI and increased NAG during postoperative period in LC(+) group suggest that UTI might play an important role in postoperative renal dysfunction in patients with liver cirrhosis.

Acetylglucosaminidase↗
PubMed1996

Clinical characteristics and biotransformation of sevoflurane in paediatric patients during antiepileptic drug therapy.

Patients with epilepsy on long term antiepileptic drug (AED) therapy deserve special consideration not only concerning seizure control but also the effect on anaesthetic metabolism and hepatorenal functions. In the present study, we examined the effects of sevoflurane anaesthesia on plasma inorganic fluoride (F-) level and hepatorenal function in patients with and without AED therapy. Twenty-two patients (12 with AEDs = AED group, and ten without AEDs = control group = C group), ASA I, who were free of hepatorenal disease, received approximately 2-3 h sevoflurane anaesthesia. Plasma F- analysis was performed at the stages of: 1) induction of anaesthesia, 2) conclusion of anaesthesia, 3) 15 h after the conclusion of anaesthesia, using an ion-selective electrode calibrated with a standard solution of sodium fluoride. Pre- and postoperative hepatic (aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, total bilirubin) and renal (blood urea nitrogen, creatinine) function was tested. There were no significant differences between the two groups in the average age (AED group = 9.4 and control group = 10.1 y.o.), body weight, duration of anesthesia, and MAC hours (2.6 and 2.4). The mean peak F- levels were 15.5 and 13.6 microM, in AED and C groups (not significant), respectively. No patient exhibited F- values greater than 50 microM, the hypothetical nephrotoxic threshold. The patients showed no abnormal values either in hepatic or renal function tests postoperatively. These results suggest approximately 2-3 h sevoflurane anaesthesia to be safe in patients taking AEDs.

Adolescent↗
PubMed1996

[Clinical analysis of 169 patients with prostate cancer].

At Matsumoto National Hospital, 169 patients with prostate cancer were diagnosed between April 1986 and May 1994. The prostate cancer incidence was the highest in the latter half of the seventies, with an average age of 74.3 years. The clinical stage was defined as A1, A2, B, C, and D2 in 24 (14.2%), 38 (22.5%), 39 (23.1%), 23 (13.6%) and 45 (26.6%) patients, respectively. The clinical stage was not correlated with the patient's age. Incidental carcinoma was discovered in 5.8% of the patients who underwent prostatectomy for benign prostatic hypertrophy (BPH). At initial diagnosis, the tumor was well, moderately, and poorly differentiated adenocarcinoma in 71 (42.0%), 64 (37.9%), and 34 (20.1%) patients, respectively. The median follow-up period was 38.7 months. The over all five-year crude survival rate was 54.8%, while the cause-specific five-year survival rate was 80.0%. The five-year crude survival rate was 58.4, 82.0, 55.2, 42.5 and 37.4%, for patients with cancer at stage A1, A2, B, C, and D. The survival rate was higher for patients with cancer at clinical stage A and shorter for those with cancer at clinical stage D than in other stages. Prognosis was also worse in patients with moderately or poorly differentiated adenocarcinoma than in those with well-differentiated adenocarcinoma. The clinical stage and the pathological grade are important as prognostic factors, although the high incidence of death from other diseases shows that the patients' age should be considered to choose the modality of therapy. These findings indicate that intensive treatment of the patients in clinical stage A2, B and C prostate cancer in combination with screening for the men between 50 and 75 years old for early cancer detection is required.

Adenocarcinoma↗
PubMed1996

[Repeated administration of protamine attenuates protamine-induced systemic hypotension].

Protamine may act on endothelial cell receptors to stimulate the production of nitric oxide, which would promote vasodilation. If so, the repeated administration of protamine may attenuate protamine-induced systemic hypotension. To confirm this hypothesis, we examined whether repeated administration of protamine attenuates protamine-induced systemic hypotension in rats. Rats were divided into two groups. In bolus injection group (S group), protamine was given intravenously at 10 mg.kg-1. In repeated administered group (R group), the same dose of protamine was given intravenously 30 min after continuous infusion of protamine 10 mg.kg-1. The mean arterial pressure in S group decreased significantly after administration of protamine, compared with R group. Also, maximal decrease rate of the arterial pressure after administration of protamine in R group was significantly lower than in S group. These results demonstrate that repeated administration of protamine attenuates protamine-induced systemic hypotension. We confirm the hypothesis that protamine will release nitric oxide by binding with the receptor on the endothelium in the vessel and subsequently cause systemic hypotension.

Animals↗
PubMed1996

[89Sr Therapy for pain relief in patients with bone metastases].

Radioactive strontium chloride (89Sr) was administered for pain relief in 6 patients with bone metastases (4 prostate cancer and 2 breast cancer patients). Out of 6 patients, 2 showed apparent relief of bone pain and improvement of QOL, and 3 showed slight relief of the pain with or without improvement of QOL; that is, 83% was effective. Side effects were seen in 2 patients; transient deterioration of bone pain in one patient and bone marrow suppression in the other patient. The patient who showed bone marrow suppression had rather more lesions of bone metastasis (diffuse metastasis) and least urinary excretion of the radioactivity. Urinary excretion for 2 days varied 5 to 40% of the administered dose and was less in the patients with more metastatic lesions.

Bone Marrow↗
PubMed1996

[Digitoxin Reference Standard (Control 951) of the National Institute of Health Sciences].

The raw material of digitoxin was tested for preparation of the "Digitoxin Reference Standard (Control 951)". Analytical data obtained were as follows: loss on drying, 0.0%; infrared spectrum, the same as that of the JP Digitoxin Reference Standard (Control 845); thin-layer chromatography, no impurity was detected; high-performance liquid chromatography (HPLC), two kinds of impurities were detected and the total amount was estimated to be 0.16 +/- 0.01% (n = 3); assay, 99.0% by HPLC. Based on the above results, the raw material was authorized as the JP Digitoxin Reference Standard (Control 951).

Chemical Phenomena↗
PubMed1996

[Betamethasone Reference Standard (Control 951) of the National Institute of Health Sciences].

The raw material of betamethasone was tested for preparation of the "Betamethasone Reference Standard (Control 951)". Analytical data obtained were as follows: loss on drying, 0.0%; infrared spectrum, the same as that of the JP Betamethasone Reference Standard (Control 845); thin-layer chromatography, no impurity was detected; high-performance liquid chromatography (HPLC), two kinds of impurities were detected and the total amount was estimated to be 0.16 +/- 0.01% (n = 3); assay, 100.0% by HPLC. Based on the above results, the raw material was authorized as the JP Betamethasone Reference Standard (Control 951).

Betamethasone↗
PubMed1996

[Tocopherol Acetate Reference Standard (Control 941) of the National Institute of Health Sciences].

The raw material of tocopherol acetate was tested for the preparation of the "Tocopherol Acetate Reference Standard (Control 941)". Analytical data obtained were as follows: infrared spectrum, the same as that of the Tocopherol Acetate Reference Standard (Control 919); specific absorbance, E1(1%)cm (284nm) = 44.5; thin-layer chromatography, no impurities were detected until 50.0 micrograms; high-performance liquid chromatography (HPLC), 2-3 impurities were detected and the amount was estimated to be about 1%; assay by HPLC, 100.8%. Based on the above results, the raw material was authorized as the Japanese Pharmacopoeia Reference Standard (Control 941).

Chemical Phenomena↗
PubMed1996

[Cyanocobalamin Reference Standard (Control 951) of the National Institute of Health Sciences].

The raw material of cyanocobalamin was tested for preparation of the "Cyanocobalamin Reference Standard (Control 951)". Analytical data obtained are as follows: loss on drying, 1.8%; infrared spectrum, the same as that of the JP Cyanocobalamin Reference Standard (Control 936); thin-layer chromatography, three impurities were detected; high-performance liquid chromatography (HPLC), eight to nine kinds of impurities were detected and the total amount of impurities was estimated to be 1.6 +/- 0.13% (n = 4); assay, 99.7% by spectrophotometry specified in the JP XII and 100.4% by HPLC, respectively. Based on the above results, the raw material was authorized as the JP Cyanocobalamin Reference Standard (Control 951).

Chemical Phenomena↗