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H Kolb

Publications and source records attributed to H Kolb.

At least 397 records · Page 22Linked to original sources

Utilisation de la centrifugation analytique pour la mesure, au sein d'un extrait brut d'E. coli, du coefficient de sédimentation du complexe de décarboxylation oxidative du pyruvate.

We have applied successfully the active enzyme analytical centrifugation method (A.E.C.) to the study of the well known pyruvate dehydrogenase complex (P.D.C.) in crude extracts from E. coli. It is not possible to study the complex in a spectrophotometer (through its dehydrogenase activity) due to NADH oxidase activities. The A.E.C. method, which overcomes this difficulty, can also be viewed as a refined spectrophotometric method.

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