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Biomedical subjects

H Kolb

Publications and source records attributed to H Kolb.

At least 379 records · Page 21Linked to original sources
PubMed1976

Carrier-dependent selectin of antibody clones and classes during the immune response.

The selection of antibody clones and antibody classes during the immune response to the hapten oligo-D-alanine was found to be carrier dependent. When the hapten was coupled to a T-independent antigen (streptococci) a restricted immune response of IgM and IgG antibodies was induced. When the hapten was coupled to a T-dependent antigen (ovalbumin) a heterogenous response of only IgG antibodies was found. The carrier had an effect on the affinity of IgG but not of IgM antibodies. Clonal pattern and class distribution were fixed early in the immune response. Second and third immunizations with a heterologous carrier elicited the same clonal pattern and class distribution in the secondary response as that found with the original carrier.

Alanine↗
PubMed1975

Horizontal cells in cat retina with independent dendritic systems.

Cat horizontal cells are retinal neurons with two functionally distinct parts: the cell body receives signals predominantly from cones, while the terminal arborization receives predominantly from rods. The long thin process connecting these parts neither generates impulses nor allows significant passive electrotonic conduction between them.

Animals↗
PubMed1975

Similar combining sites of IgG1 and IgG2a antibodies in individual mice as judged from affinity and rate constant measurements.

Within individual sera, mouse IgG1 and IgG2a subclass antibodies against the antigenic determinant oligo-D-alanine are likely to have similar combining sites. The antibodies are characterized by two methods, by their rate of binding to antigen equipped sheep red blood cells and by the hapten inhibition of antigen binding. The two characteristics are shown to be independent. The subclass antibodies of thirteen different sera differ by a factor of about 10 in their rate constant and by a factor of about 50 in affinity for hapten. In contrast, IgG1 and IgG2a within the same serum have strikingly similar rate constants (factor 1.02-1.33) as well as very similar affinities (factor 1.1-2.7). Since it is highly improbable that correlation in two independent criteria occurs by chance, the IgG1 and IgG2a antibodies are assumed to have similar combining sites.

Alanine↗
PubMed1975

Similar specificity of IgG1 and IgG2a antibodies in individual mice.

In this study the previous finding of similar variable regions in individual IgG1 and IgG2a antibody populations is extended by the demonstration of similar fine specificity of IgG1 and IgG2a combining sites. Antibody populations from individual mice directed against oligo-D-alanine determinants were analyzed in their cross-reactivity towards 5 heterologous dipeptides. This was done by mixing antibody and hapten followed by determination of free antibodies in a kinetic red cells sensitization assay. The comparison of hyperimmune sera from 10 mice showed that genetically identical mice can differ significantly in their cross-reaction pattern. Within each serum the cross-reaction pattern was determined for IgG1 and IgG2a. With a few exceptions the same individual pattern was found in both IgG1 and IgG2a antibody populations. This was taken as evidence that the combining sites of IgG1 and IgG2a anti-oligo-D-alanine antibody populations in an individual mouse are similar.

Alanine↗
PubMed1974

Rod and cone pathways in the inner plexiform layer of cat retina.

In cat retina, rod bipolar terminials do not synapse on ganglion cells but on two types of amacrine cell (types I and II). Cone bipolars synapse directly on ganglion cells and on type I amacrines. The type II amacrine appears to play a special internuncial role between bipolars and ganglion cells in the rod system.

Animals↗