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H Koide

Publications and source records attributed to H Koide.

At least 235 records · Page 13Linked to original sources
PubMed1991

Increased steady-state levels of mRNA coding for extracellular matrix components in kidneys of NZB/W F1 mice.

The present study was carried out to determine how mRNA levels of extracellular matrix (ECM) components including alpha 1(IV) chain, laminin A, B1 and B2 chains, heparan sulfate proteoglycan (HSPG), alpha 1(I) chain, and alpha 1(III) chain are regulated in the kidney of NZB/W F1 mice. Messenger RNA levels for ECM genes except for laminin A chain increased significantly with the progression of nephritis in NZB/W F1 mice. In the NZW kidneys, however, the mRNA levels for alpha 1(IV) chain, laminin B1 and B2 chains, and HSPG declined markedly with age, whereas those for alpha 1(I) and alpha 1(III) chains showed little difference throughout the experimental period. Messenger RNA levels of beta-actin remained constant, and those of laminin A chain could not be detected in either control or diseased kidneys. Immunofluorescent microscopy showed that the intensity and distribution of staining of collagen IV, laminin, and HSPG in the glomeruli of NZB/W F1 mice increased markedly with the progression of disease. Types I and III collagen were not detected in the glomeruli of NZB/W F1 mice by immunofluorescence until 24 weeks of age, after which increased amounts of these collagens were found in the glomeruli and interstitium with progression of disease. These results suggest that increased levels of mRNA coding for ECM components and increased accumulation of these proteins may contribute to a cascade of events, leading to chronic renal injury in lupus nephritis.

Animals↗
PubMed1991

[The relationship between P300 latency and regional cerebral blood flow in patients with cerebral infarction in the territory of the deep perforators].

We studied the relationship between P300 latency and regional cerebral blood flow (rCBF) in nondemented patients with cerebral infarction. Subjects were 24 nondemented patients (mean age 64.1 years) who had a CT-proven infarct in the territory of the deep perforators of the internal carotid artery system and 53 controls (mean age 64.1 years). Prolongation of P300 latency with advancing age was observed in the both groups. There was no significant difference in P300 latency and rCBF between the two groups. There was a negative correlation between P300 latency and rCBF, especially in the bilateral fronto-parietal regions in the patient group. These results indicate that cognitive function assessed by P300 latency may be related to rCBF in the fronto-parietal region in the nondemented patients with lacunar infarctions.

Aged↗
PubMed1991

Perforin mRNA expression in the inflamed tissues of NZB/W F1 lupus mice decreases with methylprednisolone treatment.

Perforin is one of the important cytolytic factors in cytotoxic T lymphocytes (CTL) and natural killer (NK) cells. In this study, the authors examined perforin mRNA levels in the kidney, spleen, liver, lung, heart, and brain of NZB/W F1 lupus mice and NZW mice. Perforin mRNA levels in the kidney, spleen, liver, and lung of NZB/W F1 mice increased significantly with age, whereas those in the heart and brain of NZB/W F1 mice showed little change between 2 and 10 months of age. In all tissues examined in NZW, control mice perforin mRNA levels showed little change during the experimental period. In addition, the authors examined the effect of methylprednisolone (MPSL) on perforin gene expression in the tissues of NZB/W F1 mice. MPSL ameliorated the increase in perforin mRNA levels in the kidney, spleen, liver, and lung of NZB/W F1 mice. These findings suggest that perforin may contribute to tissue injuries in autoimmune lupus mice and that MPSL may be effective in lupus partly by decreasing perforin expression.

Animals↗
PubMed1991

[Three cases of hyponatremia during administration of pimozide].

Three patients with hyponatremia were found at an residential home of mental developmental delay. Because pimozide had been administered to all of them, it was suggested that pimozide might have induced compulsive water drinking resulting in hyponatremia. To my knowledge, there has been no previous report that pimozide may induce hyponatremia. As children with mental developmental delay and/or autism frequently develop epilepsy, hyponatremia should be included in the differential diagnosis of convulsive seizures. Particularly when antipsychotic drugs such as pimozide have been given, we should pay attention to polydipsia, polyuria and/or general malaise and prevent hyponatremia.

Adolescent↗
PubMed1991

[Viral myocarditis].

Explore the source record for details and available documents.

Cardiomyopathy, Hypertrophic↗
PubMed1991

Modulation of basement membrane component gene expression in glomeruli of aminonucleoside nephrosis.

The present studies were designed to analyze mRNA levels of basement membrane components including collagen IV, laminin, and heparan sulfate proteoglycan (HSPG) in the course of puromycin aminonucleoside (PAN) nephrosis. mRNA levels for alpha 1 (IV) chain; laminin A, B1, and B2 chains; and HSPG were measured in glomeruli of PAN nephrotic rats 0, 2, 8, 14, and 20 days after PAN injection. In the nephrotic stage of PAN nephrosis (on the 8th day), mRNA levels for alpha 1 (IV) chain and laminin A, B1, and B2 chains increased, whereas those for HSPG decreased. The anionic sites in glomerular basement membrane stained by polyethyleneimine were smaller in size and fewer in number in PAN nephrotic rats than they were in control rats. In the remission stage of PAN nephrosis (on the 20th day), however, mRNA levels for alpha 1 (IV) chain and laminin A, B1, and B2 chains decreased, whereas mRNA levels for HSPG increased. Polyethyleneimine aggregates in this stage appeared to be larger and more intense than those in the nephrotic stage. These results indicate that the expression of basement membrane genes for alpha 1 (IV), laminin, and HSPG was abnormally regulated in PAN nephrosis and that this abnormal gene regulation might contribute to the development of proteinuria.

Animals↗
PubMed1991

[Effect of parathyroid hormone-related protein on Na(+)-dependent phosphate transport in renal brush border membrane vesicles in rats].

Parathyroid hormone-related protein (PTHrP) has significant homology with parathyroid hormone (PTH) especially in the amino-terminal sequence. We compared some effects of human synthetic analogue of amino terminal PTHrP [hPTHrP (1-34)] with those of human synthetic analogue of amino terminal PTH [hPTH (1-34)]. Intravenous administration of hPTHrP (1-34) in parathyroidectomized rats caused hypercalcemic, hypophosphatemic and phosphaturic effects in the same degree of hPTH (1-34). In the kinetic study, apparent Km and Vmax of phosphate transport in renal brush border membrane vesicles were identical in both peptides (hPTHrP(1-34) Km = 27.6 +/- 0.8 mumol, Vmax = 3.78 +/- 0.04 nmol/mg.protein; hPTH(1-34) Km = 29.6 +/- 0.9 mumol, Vmax = 3.04 +/- 0.9 nmol/mg.protein). hPTHrP(1-34) and hPTH(1-34) were equipotent in eliciting a 9-fold increase of adenosine 3',5'-monophosphate (cAMP) production. These results demonstrate that the effect of hPTHrP (1-34) is similar to hPTH(1-34) on serum calcium, phosphorus and nephrogenous cAMP in vivo, and Na(+)-dependent phosphate transport in brush border membrane vesicles.

Animals↗
PubMed1990

Protein kinase C in Saccharomyces cerevisiae: comparison with the mammalian enzyme.

Protein kinase C (PKC) was detected in the yeast Saccharomyces cerevisiae with bovine myelin basic protein as the phosphate acceptor. The enzyme was purified at least 500-fold by a four-step column chromatographic procedure (phenyl-Sepharose CL-4B, Mono Q, Heparin-5PW, and hydroxyapatite). The molecular mass was approximately 90 kDa, as estimated by gel-filtration analysis. Yeast PKC was activated by the simultaneous addition of Ca2+, diacylglycerol, and phosphatidylserine. Free arachidonic acid alone could activate the enzyme to some extent. However, yeast PKC did not respond significantly to tumor-promoting phorbol esters. GTP did not serve as phosphate donor. The yeast enzyme showed substrate specificity distinctly different from that of mammalian PKCs. H1 histone and protamine were poor substrates. With myelin basic protein as a model substrate, yeast PKC phosphorylated threonyl residues preferentially, whereas rat brain PKCs phosphorylated seryl residues preferentially. Further studies should elucidate the role of yeast PKC in cellular regulation and cell cycle control.

Amino Acid Sequence↗
PubMed1990

Preliminary study on specificity of IgA released from lymphocytes by EB virus transformation in patients with IgA nephropathy.

A study on the specificity of IgA in the supernatant of Epstein-Barr Virus (EBV) transformed lymphocytes obtained from patients with IgA nephropathy is described. Renal biopsy specimens were obtained from nine patients with IgA nephropathy and nine patients with other glomerular diseases. Mononuclear cells obtained from two patients with IgA nephropathy and two healthy adults were transformed with EBV and the supernatant of such transformed cell culture was applied to acid (pH 3.2)-treated renal sections obtained from the same and other patients with IgA nephropathy, as well as to those with other glomerular diseases. The sections were stained with FITC-labeled heavy chain-specific anti-human IgA antiserum and then examined with a fluorescent microscope. It was demonstrated that IgA in the supernatant specifically bound with the glomerular mesangial areas in patients with IgA nephropathy, while it did not bind with such areas in patients with other glomerular diseases. Although IgA in such samples bound with autologous renal tissues, about 50% bound with allogeneic renal specimens of IgA nephropathy. IgA in the supernatant of transformed lymphocytes from healthy adults was not bound with the glomerular mesangial areas of patients with IgA nephropathy and other glomerular diseases. It is concluded that IgA in the supernatant of lymphocytes by EB virus transformation is specific to the mesangial areas of IgA nephropathy and that some heterogeneity is seen among patients with this disease.

Adult↗
PubMed1990

Detection of 'activated platelets' in the urinary sediments using a scanning electron microscope in patients with IgA nephropathy.

The presence of 'activated platelets' in the urinary sediments was studied by scanning electron microscopy (SEM) in patients with IgA nephropathy. The aim of the present study was to determine if the presence of activated platelets in the urinary sediments is correlated with glomerular injuries in patients with IgA nephropathy. Eleven patients with IgA nephropathy, 5 patients with thin basement membrane syndrome and 5 patients with membranous nephropathy were examined. The presence of platelets in glomeruli was also examined by immunofluorescence (IF). This study showed a correlation between the presence of activated platelets in the urinary sediments and the severity of glomerular injuries in patients with IgA nephropathy. The platelets were observed focally in glomeruli from such patients. It was suggested that the detection of activated platelets in the urinary sediments is useful in evaluating the degree of histopathological changes and/or prognosis of IgA nephropathy.

Blood Platelets↗
PubMed1990

Plasma atrial natriuretic peptide during hemodialysis with or without fluid removal.

Plasma immunoreactive human atrial natriuretic peptide (hANP) levels were measured in 9 patients with chronic renal failure treated with maintenance hemodialysis in order to evaluate the effects of fluid removal and osmotic pressure. Under hemodialysis without fluid removal plasma hANP levels remained unchanged, but the levels were significantly decreased during extra-corporeal ultrafiltration (p less than 0.01). The present study provided strong evidence that the fall in plasma hANP levels in hemodialysis patients is mainly due to the reduction in circulating plasma volume.

Adult↗
PubMed1990

[Serum concentration of laminin in transitional cell carcinoma].

The concentration of laminin in the serum was determined for 45 patients with urinary tract transitional cell carcinoma, and the clinical significance of the findings was considered. The difference between the serum laminin concentrations in the control group and the transitional cell carcinoma patient group was not statistically significant, but the serum laminin level was highest in the transitional cell carcinoma patients with metastatic foci. In many of the patients who had metastatic foci and showed clearly progressive disease, the serum laminin concentration was found to increase with the passage of time. When the transitional cell carcinoma tissues were stained by the fluorescent antibody technique, there was little distribution of laminin in the tumor tissues, contrary to our earlier-reported findings regarding the staining of laminin in renal cell carcinoma tissues. In renal cell carcinoma patients, the serum concentration of laminin had been found to be high even when there were no metastatic foci, and consideration of this fact strongly suggests the possibility that the mechanism for laminin synthesis in urinary tract transitional cell carcinoma is different from that in renal cell carcinoma. It was surmised that the serum laminin concentration has potential for use as a diagnostic indicator of metastasis in patients with urinary tract transitional cell carcinoma.

Carcinoma, Transitional Cell↗
PubMed1990

Immunohistochemical analysis of extracellular components in the glomerular sclerosis of patients with glomerulonephritis.

Immunofluorescence and immunoperoxidase staining was carried out to determine correlations between the progression of glomerular sclerosis and changes in the amount or distribution of glomerular extracellular components, including type I, III, IV, and VI collagens, laminin and fibronectin, in patients with IgA nephropathy, membranoproliferative glomerulonephritis and rapidly progressive glomerulonephritis. Staining of type I and III collagens was not observed in glomeruli from normal individuals or patients with mild glomerulonephritis. In the advanced stages of glomerulonephritis, the staining of type IV and VI collagens, laminin and fibronectin was marked in the glomerular mesangium, and the distribution of fibronectin extended to the glomerular capillary walls in the sclerotic lesions of glomeruli. However, the staining intensity of type IV collagen, laminin and fibronectin was gradually decreased during the progression of glomerular sclerosis. On the other hand, the staining of type I and III collagens was observed focally in sclerotic or hyalinotic glomeruli and around such glomeruli in those patients. Light microscopic examination revealed that patients who showed marked staining of type I and III collagens by immunofluorescence had severe damage of Bowman's capsules. These results suggest that the hyperproduction and/or invasion of interstitial collagens, i.e., types I and III, are closely linked to the progression of glomerular sclerosis and hyalinosis in patients with various types of glomerulonephritis.

Antibodies, Monoclonal↗