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Biomedical subjects

H Koch

Publications and source records attributed to H Koch.

At least 235 records · Page 13Linked to original sources
PubMed1990

Steady-state plasma levels during antidepressant therapy with amitriptyline and amitriptylinoxide.

Plasma levels of amitriptyline, amitrypylinoxide, and their metabolites were analyzed in a controlled clinical trial with two groups of depressed patients. After administration of both drugs, demethylated and hydroxylated derivatives proved to be the main metabolites, and to a great extent amitriptylinoxide was reduced to amitriptyline. Continuous oral application of 150 mg/os per day of each drug resulted in therapeutically effective concentrations in both groups; however, concentrations were two to three times higher in the group receiving amitriptyline, rather than amitriptylinoxide. Based on the steady-state plasma levels, the total clearance of amitriptyline was calculated with a magnitude of 7 ml/min per kg. A first-order kinetic model was proposed to estimate the steady-state concentrations of consecutive metabolites, employing available kinetic data. Computed levels of nortriptyline after application of amitriptyline and amitriptylinoxide were 61.1 and 20.3 ng/ml, respectively, compared to nortriptyline levels determined with HPLC, which were 70.1 and 26.5 ng/ml, respectively. The model is discussed in relation to its practical use during monitoring of drug therapy with tricylics.

Adult↗
PubMed1990

[A clinical trial of pefloxacin (Abaktal) in the treatment of acute pyelonephritis].

The therapeutic effect of Pefloxacin in the treatment of acute pyelonephritis was examined in a randomized clinical trial. The patients in the control group have been treated by gentamycin. With a success rate of 80% in the treatment of acute pyelonephritis caused by various bacteria (E. coli, Pseudomonas, Klebsiella, Staphylococcus aureus, Proteus mirab.), a side frequency of side effects and a good compliance Pefloxacin is recommended as an effective antimicrobiell drug.

Acute Disease↗
PubMed1990

[Diagnosis and prevalence of latent hepatic encephalopathy].

To compare the efficacy of various psychometric and neurophysiological tests in the detection of latent hepatic encephalopathy (LHE) cerebral functions were studied in 146 patients with liver cirrhosis but without overt encephalopathy and in 146 matched controls. Patients with liver cirrhosis scored significantly worse than controls in 8 out of 11 tests. Best discrimination between patients with cirrhosis and controls was obtained by testing for reaction time to white and colored light with a reaction time apparatus (DTG), and with the digit symbol (UT1) and block design test (UT4), i.e. with two Wechsler adult intelligence scale performance tests. Thirtyseven out of 146 (25%) patients with cirrhosis reveiled an abnormal result with the DTG alone. A combination of the DTG, UT1 and UT4 yielded the diagnosis in 44 (30%) patients. LHE correlated with the severity of the disease (Child-Pugh classification) but not with its etiology or with portasystemic shunting. In the Federal Republic of Germany about 300,000 subjects suffer from liver cirrhosis. Based on our results 100,000 of them may have LHE.

Adult↗
PubMed1990

[The dynamics of the microbial count in experimentally-induced salmonellosis of calves].

Numerical developments of pathogens were followed up in intestinal matter, mucosa, and lymph nodes as well as in spleen and liver of calves which had been orally infected with Salmonella (S.) dublin or S. typhimurium. Massive flooding of the organism with Salmonellae and eventually fatal proliferation were found to be possible immediately after infection or after some days of pathogen-host equilibrium. Complete elimination of Salmonellae was found to be achievable even after infestation of spleen and liver. Intestinal lymph nodes, in addition to intestinal mucosa and intestinal matter, proved to be of decisive relevance to pathogenesis of the infection and to persistence of germs.

Animals↗
PubMed1989

Changes of immunological parameters in immunocompromised patients under selective decontamination of the digestive tract. Third Part: Behaviour of some plasma proteins.

We studied the importance of changes in plasma protein concentrations in patients with hematologic systemic diseases treated by selective decontamination of the digestive tract. The concentrations of albumin, acute-phase proteins, immunoglobulins and fibronectin were determined by laser nephelometry in 125 serum samples of eight patients. Results obtained allow the following conclusions: 1. There was a correlation between the clinical condition and concentrations of acid alpha-1-glycoprotein, IgG, CRP and fibronectin. These concentrations might be important for assessment of the course of the disease, effectiveness of therapy and the detection of pathogenetic relations. 2. The acid alpha-1-glycoprotein is of particular importance since relations exist between changes in its concentration and therapeutic results as well as the prognosis of the disease.

Acute-Phase Proteins↗
PubMed1989

Changes of immunological parameters in immunocompromised patients under selective decontamination of the digestive tract. Second Part: Investigations on the influence of drugs on zymosan-induced and luminol-enhanced chemiluminescence of peripheral leucocytes.

The influence of drugs used for selective decontamination, given in therapeutically effective concentrations, was examined in healthy controls and immunocompromised patients with hematologic systemic diseases by measuring the zymosan-induced and luminol-enhanced chemiluminescence of peripheral leucocytes. Drug-induced repression of phagocytic activity could usually be found both in healthy controls and in patients with hematologic systemic diseases. The combination of trimethoprim and sulfamerazine had a pronounced repressive effect. Such indications should be taken as a basis for further investigations in order to avoid additional iatrogenic restriction of defence. If possible, drugs with effects leading to repression of phagocytosis should not be used for selective decontamination.

Anti-Infective Agents↗
PubMed1989

Selective decontamination of the digestive tract in hematological patients (Czechoslovakia-German Democratic Republic cooperative study.

A group of 55 hematological patients treated for the last 2.5 years by the method of selective decontamination was evaluated. Though both institutes (Bad Saarow, Hradec Králové) worked on the problem in the same conditions (indications for the treatment, characteristics of patients, basic drugs), many differences in details were found. However, the important clinical results were the same: A statistically significant decrease in infections and duration of fever in treated patients. A survey of therapy complications, surveillance of infections and incidence of microbes are presented. The evaluation showed that future research including microbiological and immunological investigation based on a standard protocol will be useful.

Adult↗
PubMed1989

Rumen dry matter degradability and apparent digestibility of variously treated rice hulls.

Rice hulls from Hungary, Colombia, and Cambodia were used for measuring with the nylon bag method the dry matter degradability in the rumen of sheep. After 48 h rumen incubation time the dry matter loss varied between 10.0 and 27.7%. The 48 h rumen dry matter loss of rice hulls from Hungary increased from 14.0 (untreated control) only to 17.9, 20.1, and 17.0% after chemical treatment with 2% NaOH, 4% CaO, and 4% urea, respectively. gamma-irradiation with doses of 0, 0.1, 0.25, 0.5, 1, and 2 MGy effected rumen dry matter losses of unground rice hulls from Hungary of 6.4, 14.5, 13.5, 21.3, 37.1, and 70.5%. The apparent digestibility of organic matter of untreated rice hulls from Hungary amounted to 19.2%, as found in tests with wethers. The main reasons of low digestibility and the negligible effect of chemical treatments are the high contents of lignin (ca. 15%) and silica (up to 23% of the dry matter). Therefore rice hulls are not suitable as feedstuff for ruminants.

Animal Feed↗
PubMed1989

[The pathomorphology of the liver after unilateral hepatic duct obstruction with laboratory-chemical observations of the course in the dog].

UNLABELLED: In an experimental study the morphological and functional changes of the liver with unilateral hepatic duct obstruction were investigated over a period of 13 months. In 4 series different parts of the liver were excluded of the bile drainage by hepatic duct ligation after cholecystectomy (group I = 25%, group II = 50%, group III 75%, group IV = 100% of the liver, series V = control group was cholecystectomy only. The clinical outcome, biochemical parameters, liver biopsy were examined regularly. Bacteriologic investigation of the bile and hepatic flow measurement were performed at the beginning and at the end of the study. RESULTS: The clinical symptoms were discrete and the biochemical parameters showed a typical course. After 6 weeks, atrophy of the excluded liver with contralateral compensatoric hypertrophy was found. The microscopic correlation was the secondary sclerosing cholangitis (SSC). After 6 weeks, a concentric periductal fibrosis was to be observed in the periportal area. After 12 weeks, bile duct vanishing with persistence of the arteries and veins was found. After 36-48 weeks, biliary cirrhosis and total destruction of the liver parenchyma was found respectively. Simultaneously a chronic disturbance of the hepatic perfusion was seen. It was caused by a perivenous fibrosis of the terminal vein with obliteration of the lumen by endangiitic proliferations and cavernous transformation. The genesis of SSC seemed not be be influenced by bile contamination. The ligated as well as the unligated bile ducts were infected in 20-50% only. There was no difference in the liver specimens with sterile or contaminated bile. The hepatic flow measurement showed a reduction of the portal blood flow and a rise of the arterial flow depending on the amount of the excluded liver. A better understanding of the pathophysiological sequelae of the unilateral hepatic duct obstruction suggests that the drainage by surgical or radiological methods may not invariably be necessary.

Animals↗
PubMed1987

Interphase cell flow cytometry as a means of monitoring genomic size in normal and neoplastoid cell cultures.

The DNA specific fluorescence of mass cultures and clones derived from human skin and bladder tumor tissue was assayed by flow cytometry. In order to detect and quantitate small fluorescence intensity changes, cytogenetically defined triploid or diploid human fibroblast strains were cocultivated, harvested, and stained with the cell strain of unknown karyotype. The triploid standard (derived from human abortus tissue) proved chromosomally unstable at high passage level. Fifteen male, female, and 45,X strains displayed target-to-standard cell fluorescence ratios commensurate with their respective chromosome constitutions. Interstrain variation was highest among the 45,X strains, although mosaicism could not be detected by conventional cytogenetics. Interclonal fluorescence variation was two- to ten-fold higher among the tumor-derived clones tested. Chromosome counts and subcloning experiments indicate that this increased fluorescence variation is due to genome size variation. The clonal evolution of genome size differences was observed in subclones of chromosomally divergent parental clones. These observations suggest that well controlled flow cytometry can adequately resolve subtle degrees of genome size variation in cultivated human cells. The technique is especially suited for monitoring genome size changes in cultivated tumor cells.

Cell Separation↗