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Biomedical subjects

H Klosterhalfen

Publications and source records attributed to H Klosterhalfen.

At least 19 recordsLinked to original sources
PubMed1992

Transplantation of human benign hyperplastic prostate tissue into nude mice: first results of systemic therapy.

Xenografts of human benign hyperplastic prostate tissue (BPH) have been established as a model for the investigation of the etiology of BPH. In this paper it is our aim to answer the question of whether this model is useful for the established therapy of the disease. Additionally we try to evaluate the value of a commonly used plant extract for the therapy of BPH. Is there any influence of the extract from Sabal serrulatum on the BPH tissue in our model? Human BPH tissue from 2 patients was transplanted into athymic nude mice and treated with three different regimens. Animals of group I did not receive any treatment and served as control, in groups II and III the tissue was stimulated by application of silicone tubes containing crystalline 5 alpha-dihydrotestosterone and estradiol. Animals of group II additionally were treated orally with the lipophilic extract of S. serrulatum (contained in Prostagutt N), which is commonly used for the treatment of BPH clinically. Significant inhibition of tissue growth was observed in group III when compared to group II. In group I (control) atrophy of the graft was observed as expected. However, histologically no differences were visible between groups II and III. Our experiment shows a significant growth-inhibiting effect of the plant extract for human BPH tissue in our model (p less than 0.05). We conclude that the nude mouse model can be useful for the evaluation of systemic therapy modalities of human BPH and that the plant extract may have a certain value for clinical treatment, which is not only due to subjective criteria.

Animals↗
PubMed1992

[Obstructive uropathy after kidney transplantation. Experiences with endourologic incision of ureteral stenoses].

Between 1985 und March 1991 we have managed 8 upper urinary tract obstructions in kidney transplants using an endourological approach. After a graft rejection was excluded an obstruction was initially diagnosed by nephrosonography and further confirmed by IVP or antegrade pyelography. To investigate the urodynamic relevance of the stenosis, all patients underwent preoperative diuretic isotope renography. In all cases a percutaneous pyelostomy was done to preserve renal function. 7 of these 8 patients demonstrated a stenosis of the ureter, while in one case, the obstruction was caused by a coagulum in the renal pelvis. Incision of the stricture then was performed with a flexible knife antegrade or retrograde and stented for 4-6 weeks. In 6 out of 7 cases with a proven stenosis of the ureter, the cold knife incision lead to a successful outcome, while in one patient, the kidney had to be removed due to uncontrolled bleeding 12 days after successful percutaneous incision. Our results indicate, that the cold-knife-technique for the management of upper urinary tract obstructions in kidney transplants is a promising, fast and in most of the cases effective method. Due to its minimalinvasive character and excellent results, this approach is able to replace open reintervention in most cases.

Adolescent↗
PubMed1992

[Vasovasostomy after vasectomy. The surgical results 1986-1989].

The frequency of vasovasostomy has increased dramatically in recent years. A significant percentage of men who have previously undergone vasectomy are now seeking restoration of their fertility. The most common reason for requesting a reversal is remarriage. There were 90 bilateral vasovasostomies performed in 90 patients between 1986 and 1989. The procedure was done according to the technique described by Howards, in a single layer with 7 x 0 prolene and under fourfold magnification. The success rate was 87% for presence of spermatozoa in the ejaculate, 48% for pregnancy. The fertility rate decreases slowly with increasing interval between vasectomy and reanastomosis. These results are statistically significant. In cases of good anatomical and andrological conditions, the chance of restoration of fertility is good. The experience of the urologist is often more important than the details of the technique.

Adult↗
PubMed1990

Monoclonal antibody to the prostate specific antigen.

Somatic cell hybrids were made from mouse myeloma cells and spleen cells derived from BALB/c mice immunized with homogenized epithelial fractions of BPH. The screening by immunoperoxidase staining on human prostate and non-prostate tissue resulted in one monoclonal antibody identifying a prostate specific antigen. Upon SDS-PAGE and Western blot this antigen exhibited a single band at the position of 34 kDa molecular weight. The immunoreactivity of the prostate antigen was found to be localized exclusively in the epithelial lining of ducts and secretions of normal prostate, BPH and prostate cancer. Anti-p34 antibody reacted with an antigenic determinant on the PSA molecule cancer. Anti-p34 antibody reacted with an antigenic determinant on the PSA molecule and inhibited the binding of Anti-PSA antibody to PSA by about 80 to 90% in the RIA.

Adult↗
PubMed1979

[Do biopsies and operations lead to formation of distant metastases? (author's transl)].

In medical and public literature the radiologist Krokowski states that diagnostic and operative procedures of cancer disease often stimulates the development of metastases. He believes that 30--90% of metastatic disease is the result of prior operations. Consequently he requires a prophylactic radiation therapy before any operative therapy is done. The authors question the theses of Krokowski since there is no detail presentation of this methods, which therefore cannot be repeated.

Biopsy↗
PubMed1979

[Treatment of prostatic carcinoma stage C with combined perineal and transurethral cryosurgery (author's transl)].

31 patients with prostatic carcinoma stage C were treated by combined perineal and transurethral cryosurgery. Additionally in all patients a subcapsular orchiectomy was performed. A distinct tumour reduction was found in all patients. Six months after the freezing a palpable tumour could be detected only in 4 cases out of 29. At different time intervalls after the operation 57 biopsies of the prostate were obtained with the tru-cut-needle. In 14 patients no tumour tissue could be detected whereas tumour cells were found in the biopsies of the remaining patients. Combined perineal and transurethral cryosurgery therefore is a palliative form of treatment in prostatic carcinoma.

Aged↗
PubMed1979

[Pathogenesis and therapy of varicocele (author's transl)].

During the last 13 years 519 patients were operated upon for varicocele in the refertilisation center Hamburg. Out of 209 cases examined thereafter, 174 showed an improved spermiogram, and only in this group 40 children were fathered. Pathogenesis, diagnosis and surgical treatment are presented. A persisting varicocele (in our clientele in 1 per cent) was corrected in 18 patients. The reasons for recurrent surgery were overlooked branches of the V. spermatica interna and not yet described collaterals to neighbouring venous systems. For optimal surgical planning the latter are best demonstrated by preoperative phlebography.

Adolescent↗
PubMed1978

[Open prostatic surgery in patients over 80 years (author's transl)].

The fact that a patient needing prostatic surgery is over 80 years old need no longer be a reason why open surgery should not be considered in the treatment programme of the general practitioner or internal physician. Out of 45 men aged between 80 and 92 only two died as a result of surgery. Life expectancy is not reduced by the operation.

Age Factors↗
PubMed1978

[A new substance for the therapeutic embolisation of renal tumours (author's transl)].

We report on a radio opaque substance for the embolization of kidney tumours which consists of amino acids mixed up with a contrast medium. It is relatively easy to be injected and can be well directed because of its radio-opacity. Past and recent histological examinations of embolized pig kidneys show that this material provides a durable closure of the vessels and that there is no essential tendency of intravasal dissolution. It is therefore also suitable for palliative embolizations. The first results in clinical trial have proved to be encouraging.

Amino Acids↗
PubMed1978

[Occult reflux (author's transl)].

Level diagnosis repeatedly performed in patients without roentgenologically demonstrable reflux demonstrated bladder bacteriuria in 80% of the cases. The remaining 20% had supravesical bacteriuria. We called this occult reflux, if reinfection was demonstrated. Contamination of the upper tract by occult reflux can, but must not induce pyelonephritis. Bilateral antireflux surgery frequently eliminates occult reflux of bacteria, so this seems a debatable method of treatment.

Adolescent↗
PubMed1978

[The bilateral nephrectomy in terminal renal disease (author's transl)].

45 cases of nephrectomy are reported. Mortality was 8.9%. Thirty-one had a drug-resistant, severe hypertension, three had infected kidneys, and in eleven cases nephrectomy was performed prior to transplantation. Although blood pressure improved in all patients, of the first group 24% died within one year after nephrectomy, which shows unsatisfactory prognosis of malignant nephrosclerosis. Only early nephrectomy can lead to better results. Routine nephrectomy before transplantation should be avoided. Indications for this group are discussed.

Adult↗
PubMed1978

[Functional efficiency of the vesico-ureteral closure mechanism in female patients with recurrent urinary tract infections without reflux].

On account of different relapses of infections in 15 female patients with relapsing non-obstructive infections of the urinary tract without radiologically provable vesico-ureteral reflux altogether 183 washing out tests of the urinary bladder were performed. 83.6% of the tests resulted in a pure bacteriuria of the bladder and 14.2% in a supra-vesical participation. Of the 51 female patients 38 = 74,5% were always suffering only from bacteriurias of the bladder. In 13 patients = 25.5% we also or always found a supravesical participation. 15 female patients underwent a reflux test with technetium-levelled sulphocolloid. In no patient the tracer could be localised in the supravesical urinary tract. By reason of these results we are of the opinion that in the majority of female patients the vesico-ureteral occlusion mechanism does not allow an ascent of the germs. Therefore, the majority of patients is not imperilled by pyelonephritis. Among the female patients with relapsing non-obstructive infections of the urinary tract without radiologically provable reflux are some with marginally competent ostia. In these more or less frequently an occult reflux with ascent of the germs into the supravesical urinary tract may develop. These patients are potentially imperilled by pyelonephritis.

Adolescent↗