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Biomedical subjects

H Kitamura

Publications and source records attributed to H Kitamura.

At least 505 records · Page 28Linked to original sources
PubMed1984

The functional characteristics of cultured rat mesangial cell.

Glomerular mesangial cells were isolated from explants of normal rat whole glomeruli according to the differential growth capacities of the cultured cells. The cultured mesangial cells comprised almost all of the grown cells on day 21 of the primary culture when the subculture could be performed. They contained abundant cytoplasmic microfilaments and actin. The contractile response to vasoactive hormone of both primarily cultured and subcultured mesangial cells was quantified as a decrease in cell surface area. The maximum decrease in surface area in response to 1 nM of angiotensin II was about 30%. This magnitude of contraction is consistent with that observed in cultured rat vascular smooth muscle cells. The mesangial cells did not phagocytose horse-radish peroxidase or sensitized sheep red blood cells, nor did they have binding capacities for Fc or C3b, while the cells derived from explanted glomerulus and probably of blood-born monocyte origin did. We conclude that the most important function of the glomerular mesangial cell is the control of glomerular blood flow and filtration by contraction.

Angiotensin II↗
PubMed1984

Simultaneous changes of left ventricular and left atrial size and function in normal subjects during exercise.

We performed dual M-mode exercise echocardiography, which records left ventricular (LV) and left atrial (LA) echocardiograms simultaneously, in 10 normal subjects to investigate mechanisms of cardiac adaptation to dynamic exercise. The LV end-diastolic dimension (EDD) increased significantly as exercise increased from mild to moderate (3-5%, p less than 0.05 and 0.001). At peak exercise the EDD was slightly increased, but it returned to the baseline during recovery. The LV end-systolic dimension (ESD) showed a progressive decrease with the severity of exercise (5-13%, p less than 0.05 and 0.001). It decreased further immediately after exercise (17-20%) and then returned to the resting value. The LV stroke dimension and fractional shortening increased as exercise changed from mild to moderate and reached a plateau at peak exercise. During recovery, they showed a transient increase and then decreased. By contrast, the LA dimensions, both maximum and minimum, increased significantly as exercise changed from mild to moderate (15-16% and 16-19%, p less than 0.01 and 0.001, respectively), but they were lower at peak exercise (12 and 14%). They returned rapidly to the resting values immediately after exercise. Thus, during exercise, LV function is augmented by the Frank-Starling mechanism in combination with increased contractility, while the LA is enlarged to receive the increased venous return accompanying dynamic exercise. These exercise-induced changes in LV performance return gradually to the resting state, but LA size recovers rapidly after the cessation of exercise.

Adolescent↗
PubMed1984

[A case of papillary adenocarcinoma of the prostate].

A relatively rare case of papillary adenocarcinoma of prostate is reported. The patient was a 49-year-old male. He presented with the chief complaints of micturition frequency, a feeling of residual urine and a burning sensation in urethra. There was no palpable abnormality in the prostate. Endoscopic examination revealed papillary tumors in the prostatic urethra and the anterior urethra, but no evidence of abnormality in the utricle. Both hematological examination and blood chemistry revealed no abnormalities; ACP, PACP and PAP were normal. The tumors were resected by TUR on March 8, 1982. Histopathological findings indicated papillary proliferation of single layer of columnar epithelium, with clear cytoplasm and nuclei with atypism distributed in the base. The tumors in the anterior urethra gave the same findings, and the diagnosis made was papillary adenocarcinoma which seemed to have originated from the prostatic duct. After operation, hormone therapy and chemotherapy with peplomycin were conducted. Eleven months after the operation, the remaining prostatic tissues including surgical capsule were resected as completely as possible. Histopathological findings revealed only atrophic prostatic tissues without any remaining tumor. The hormone therapy was discontinued. Presently, 19 months has elapsed since the first TUR. His micturition condition is good without evidence of recurrence or metastasis.

Adenocarcinoma, Papillary↗
PubMed1984

C3-independent immune haemolysis: haemolysis of EAC14oxy2 cells by C5-C9 without participation of C3.

C3-independent immune haemolysis was studied using EAC14oxy2 cells and purified C5, C6, C7, C8 and C9. We have found that EAC14oxy2 cells were lysed by C5-C9 and that haemolysis occurs, even after pretreatment of the cells and the C5-C9 preparation with anti-C3. This indicates that EAC14oxy2 can be lysed by C5-C9 without any participation of C3. In contrast, EAC1 and EAC14 cells are not lysed by C5-C9, suggesting that our C5-C9 preparation lacks activated complement components, such as C3bBb, C5b6 or C(56)a. Based on our study of the haemolysis of EAC14oxy2, we have determined that: (i) EAC14oxy2 cells are lysed by a preparation of C5, C6, C7, C8 and C9, but no lysis occurs when any one of these complement is absent, (ii) for significant haemolysis of EAC14oxy2, a higher concentration of C5 is necessary as compared to the C5 requirement when haemolysis occurs in the presence of C3, (iii) the degree of haemolysis is linearly related to the concentration of C5 and does not reach a plateau, despite the addition of as much as 3,200 U of C5, and (iv) the degree of haemolysis is linearly related to the concentration of cell bound C42. These observations suggest that, in the absence of C3, the C3 convertase C42 can activate C5 directly, resulting in the formation of the membrane attack complex, C5b-9.

Complement C2↗
PubMed1984

[A case of renal adenoma associated with staghorn calculus].

A case of renal adenoma associated with staghorn calculus is reported. The patient was a 52-year-old man. In 1973, he underwent an ileal conduit because of tuberculous contracted bladder and impediment in renal function. At that time, small calculi were already present in the right kidney. Later, in the left kidney, calculi developed into staghorn calculus. An ureteral calculus was also present in the right side and contracted kidney was observed. In 1983, he suffered from continuous left abdominal pain and macroscopic hematuria. Left nephrolithotomy was performed on August 23, 1983. During surgery, a yellow subcapsular flat mass the size of the tip of an index finger was found in the upper pole of the left kidney and was easily excised. An 11 cm incision was made in the renal parenchyma and the calculi were completely removed. At the center of the incised region, biopsy was performed. The wound was closed by the one-layer interrupted parenchymal suture method designed by Taguchi. Pedicle clamp time was 37 minutes and postoperative macroscopic hematuria was seen for only two days. The postoperative course was uneventful. The resected tumor was 18 X 16 X 10 mm in size. Histological examination revealed presence of fibrous capsules and papillary or tubular proliferation of cells which were suspected to have originated in the renal tubule. Also, there were scattered nests of foam cells. On the basis of these histological findings, the mass was diagnosed as renal adenoma. In addition, examination of the biopsied parenchyma also revealed small multiple adenomas, which were supposed to be remaining in the left kidney.

Adenoma↗
PubMed1984

Study on C3-like factor in the serum of a C3-deficient subject.

Analysis of the serum of an individual having a deficiency of C3 (C3D) revealed that despite the fact that the C3 could not be detected immunochemically, a small amount of C3 haemolytic activity and total complement activity was indeed found to be present. The present study was performed to analyse the C3-like factor, responsible for this C3 activity in C3D serum. It was found that the C3-like factor was not neutralized by anti-C3 but was neutralized by anti-C5. The electrophoretic mobility of the C3-like factor corresponded to that of purified C5. Thus, C3-like factor was shown to have the antigenicity and electrophoretic mobility of C5. This factor was found in the sera of three other C3D subjects and even in pooled normal human serum (NHS). These findings suggest that, in cases of C3D, C5 compensates for the genetic lack of C3 and serves a protective function and that sensitized erythrocytes (EA) may be lysed by complement components without participation of C3.

Complement C3↗
PubMed1983

A new working formulation of non-Hodgkin's lymphomas. A retrospective study of the new NCI classification proposal in comparison to the Rappaport and Kiel classifications.

Two hundred thirty cases of malignant non-Hodgkin's lymphomas were reclassified in a retrospective study according to the New Working Formulation for Clinical Usage of the NCI as compared to the Rappaport and Kiel classifications. The reproducibility for the individual schemes this study was 81% (Rappaport), 79% (Kiel), and 85% (New Working Formulation). In keeping with the results of the NCI international study, all lymphomas were subdivided into 3 prognostic groups: (1) low-grade malignancy (6.0 years median survival); (2) intermediate-grade malignancy (3.5 years median survival); and (3) high-grade malignancy (1.4 years median survival). The NCI-proposed New Working Formulation for Clinical Usage is thus recommended as practical and unprejudicing classification scheme for general application; however, its usefulness as tool for translating one classification scheme into another appears limited.

Europe↗
PubMed1983

Induction of indoleamine 2,3-dioxygenase in alveolar interstitial cells of mouse lung by bacterial lipopolysaccharide.

The cellular localization of indoleamine 2,3-dioxygenase was studied in the mouse lung after induction by lipopolysaccharide treatment. No significant indoleamine 2,3-dioxygenase activity was detected in alveolar macrophages and type II epithelial cells, which were recovered by alveolar lavages and trypsin-treatment, respectively. To determine this enzyme activity in other types of lung cells, we prepared monodispersed lung cells (6.5 X 10(7) cells/lung) by incubation with 0.1% collagenase and 0.1% trypsin. In a Percoll isopycnic gradient, the dispersed cells were distributed with two peaks at the densities of 1.040 and 1.080 g/ml. The enzyme activity was recovered exclusively in the lighter fractions. As examined by electron microscopy or more quantitatively by using various marker enzyme activities, endothelial cells (angiotensin-converting enzyme as a marker enzyme of these cells), alveolar interstitial cells (prostaglandin dehydrogenase), type I epithelial cells, type II epithelial cells, alveolar macrophages (beta-glucuronidase), Clara cells (coumarin hydroxylase), and polymorphonuclear leucocytes (arylsulfatase) were distributed with peaks at the densities of 1.033, 1.040, 1.042, 1.045, 1.070, 1.082, and 1.093 g/ml, respectively. The distribution pattern of the indoleamine 2,3-dioxygenase activity exactly coincided with that of alveolar interstitial cells. The localization of this enzyme in alveolar interstitial cells was immunohistochemically confirmed with the anti-indoleamine 2,3-dioxygenase antibody.

Animals↗
PubMed1983

Actin filaments in the ameloblast of the rat incisor.

The distribution of actin filaments in the inner enamel-secretory ameloblast of the rat incisor was examined by labelling with heavy meromyosin. Actin filaments were mainly located in the long axis of the cell beneath the cell membrane. They tended to be more numerous in Tomes' process than in the cell body. Some filaments were associated with microtubules, with other actin filaments, and coated vesicles. Possible relation of the actin filaments to cell motility, stabilization of some, and movement of other cellular components is discussed.

Actins↗