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Biomedical subjects

H Kindahl

Publications and source records attributed to H Kindahl.

At least 163 records · Page 9Linked to original sources
PubMed1987

15-Keto-13,14-dihydroprostaglandin F2 alpha and prolactin in maternal and cord blood during prostaglandin E2 or oxytocin therapy for labor induction.

15-keto-13,14-dihydroprostaglandin F2 alpha plasma levels were measured in pregnant women following labor induction with either oral PGE2 treatment or intravenous oxytocin, both combined with amniotomy. The median time to start of contractions was 62 minutes in the PGE2 treated group and 45 minutes in the oxytocin treated group (p less than 0.01; median test). The increase in 15-ketodihydro-PGF2 alpha appeared earlier in the PGE2 group but not in the oxytocin group (p less than 0.001 and p = 0.210, respectively). At delivery, the 15-ketodihydro-PGF2 alpha values had further increased in both treatment groups. The increase was significantly higher in the PGE2 treated patients compared with oxytocin treated patients (p = 0.03; contrast test). Despite higher 15-ketodihydro-PGF2 alpha concentrations throughout parturition, PGE2 women did not deliver more rapidly than oxytocin infused women. There was no correlation between 15-ketodihydro-PGF2 alpha blood concentrations and either onset of contractions or labor time. The decrease in maternal serum prolactin concentration during parturition was pronounced (p less than 0.001) in the PGE2 group but occurred also in oxytocin treated patients (p less than 0.02). A single oral dose (0.5 mg) of PGE2 taken by non-pregnant women led to significant (p less than 0.05) increases in 15-ketodihydro-PGF2 alpha levels in blood plasma after 10 minutes. This increase persisted for at least 90 minutes. It is suggested that oral PGE2 may be transformed into PGF2 alpha and/or induce endogenous PGF2 alpha biosynthesis.

Adult↗
PubMed1987

Plasma concentrations of vasopressin and a prostaglandin F2 alpha metabolite in women with primary dysmenorrhoea before and during treatment with a combined oral contraceptive.

Oral contraceptives reduce menstrual pain but the interaction with vasopressin and prostaglandin F2 alpha, two uterine stimulants related to the condition, is unknown. Ten women with a history of moderate to severe dysmenorrhoea were studied. Repeated blood samples were taken during a first menstrual cycle without treatment, during the first 21 days of a second cycle when they received an oral contraceptive (150 micrograms levonorgestrel and 30 micrograms ethynyloestradiol) and on the first or second day of the bleeding following hormonal withdrawal. Measurements were made of plasma concentrations of arginine vasopressin, 15-keto-13,14-dihydroprostaglandin F2 alpha, oestradiol-17 beta, progesterone, ethynyloestradiol, levonorgestrel, FSH, LH and prolactin, and serum osmolality was measured. Seven of the women rated their discomfort as moderate to severe on the first two menstruations, but as none or light at the withdrawal bleeding; with the rating scale for degree of pain that was used, this decrease in pain was significant (P less than 0.001). The plasma concentration of vasopressin in these seven women showed significant variation, with the highest concentrations being obtained at the beginning of the two painful menstruations (3.76 +/- 0.76 and 1.75 +/- 0.30 (S.E.M.) pmol/l) and at ovulation in the control cycle (1.91 +/- 0.58 pmol/l). During treatment the concentrations were consistently low, except on the first day of withdrawal bleeding (2.33 +/- 0.35 pmol/l). The concentrations of the prostaglandin F2 alpha metabolite showed less variation, but again the values at withdrawal bleeding (271 +/- 39 pmol/l) were not different from those obtained over the painful menstruations (255 +/- 24 and 217 +/- 25 pmol/l).(ABSTRACT TRUNCATED AT 250 WORDS)

Adolescent↗
PubMed1987

Hormonal changes associated with induced late abortions in the mare.

Two mares received PGF-2 alpha twice daily until abortion and 2 mares received a combined treatment with oestradiol benzoate and oxytocin. The mares were about 150 days pregnant. The PG-treated animals aborted after 37 and 61 h, respectively, and the fetuses were expelled in intact fetal membranes. The other 2 mares aborted 13 and 27 h after the first oxytocin injection, respectively, and showed strong uterine contractions and expelled the fetuses in disrupted fetal membranes. Concentrations of 15-ketodihydro-PGF-2 alpha increased both after PG and oxytocin injections and in association with the abortion, but after the PG-induced abortion there was an immediate return to basal levels and after the oxytocin-induced abortion there was a large increase in the concentrations, indicating damage of the uterus. Progesterone and relaxin concentrations followed the placental function and decreased in association with the abortions. Oestrone sulphate values differed in the two groups; the oxytocin-treated animals showed a rapid decrease while the mares treated with PG showed first a marked increase and then a decrease. Concentrations of PMSG appeared to be unaffected by the abortions.

Abortion, Induced↗
PubMed1987

Effect of Salmonella typhimurium endotoxin on PGF-2 alpha release and fetal death in the mare.

The infusion of Salmonella typhimurium endotoxin into pregnant mares resulted in a biphasic release pattern of PGF-2 alpha as determined by 15-keto-13,14-dihydro-PGF-2 alpha concentrations. The initial phase of 1 h duration was followed by accentuated release by 2 h after infusion; concentrations reached basal levels by 6 h. In 7 mares at 23, 26, 29, 33, 36, 53 and 55 days of gestation, fetal death occurred between 36 and 120 h after infusion; 12 mares at 46, 51, 56, 59, 65, 71, 73, 85, 103, 138, 283 and 318 days of gestation did not abort after endotoxin infusion. Luteal activity was compromised in all mares by 9 h after infusion. Progesterone concentrations were consistently lower in mares that aborted (1-2 ng/ml) than in those that did not abort. Mares therefore appear to be vulnerable to fetal loss by a clinical syndrome induced by Salmonella typhimurium endotoxin until about 50-60 days of gestation.

Animals↗
PubMed1987

Management of twin conceptuses by manual embryonic reduction: comparison of two techniques and three hormone treatments.

One hundred mares carrying twin conceptuses between gestation days (GD) 12 and 30 were assigned to 4 groups. Group-1 mares (n = 20) were given a placebo (sterile saline solution, IV, and sesame oil, IM). Group-2 mares (n = 32) were given a single dose of progesterone (625 mg, IM) in sesame oil. Group-3 mares (n = 28) were given multiple progesterone treatments (625 mg, IM) at 6-day intervals until GD 42. Group-4 mares (n = 20) were given a single treatment of flunixin meglumine (500 mg, IV) and progesterone (625 mg, IM). Each group was further assigned to equal subgroups A and B according to the age of the embryos. In subgroup A, with embryos at GD 12 to 16, embryonic vesicle rupture was performed after the selected conceptus was moved to the tip of a uterine horn. In subgroup B, with embryos at GD 17 to 30, embryonic vesicle rupture was performed on the selected conceptus in situ. An additional 6 mares carrying single conceptuses (GD 12, 14, 16, 20, 25, and 30) were used as subjects for sham embryonic vesicle rupture. Both rupture techniques usually caused the release of prostaglandin (PG) F2 alpha for up to 90 minutes. Amounts of PGF2 alpha were directly correlated with the pressure required to effect embryonic vesicle rupture. Sham embryonic vesicle rupture attempts caused small PGF2 alpha releases after each manipulation, which rapidly returned to basal values. All 6 mares were still pregnant at GD 42. Flunixin meglumine inhibited PGF2 alpha release after embryonic vesicle rupture, regardless of the technique.(ABSTRACT TRUNCATED AT 250 WORDS)

Animals↗
PubMed1986

Endotoxin-induced and prostaglandin-mediated effects on corpus luteum function in the mare.

Intravenous administration of endotoxin to mares causes synthesis and release of PGF(2alpha); this process affects corpus luteum function, either causing a transient drop in progesterone levels or complete luteolysis with shortening of the estrous cycle. It seems likely that any disease associated with endotoxemia can cause important prostaglandin-mediated effects on reproductive function in the mare.

Journal Article↗
PubMed1986

Endotoxin-induced bovine mastitis: arachidonic acid metabolites in milk and plasma and effect of flunixin meglumine.

Arachidonic acid metabolites (AAM) were measured in milk and plasma during the course of acute endotoxin-induced mastitis in 12 lactating cows. Mastitis was induced by intramammary challenge exposure with 10 micrograms of Escherichia coli (026:B6) endotoxin. Endotoxin was injected into the teat cistern via the teat canal of a single randomly selected rear quarter of each cow. Concentrations of prostaglandin (PG) F2 alpha and thromboxane (Tx) B2 in fat-free unextracted milk and of 15-keto-13,14-dihydro-PGF2 alpha in plasma were measured by radioimmunoassay. Total production of AAM in milk was determined by measuring quarter milk production. The AAM were compared in 6 cows administered flunixin meglumine (1.1 mg/kg of body weight) and in 6 cows administered saline solution. Concentrations of TxB2 in milk were significantly (P less than 0.001) increased during the early course of acute mastitis in endotoxin-treated quarters of cows not administered flunixin meglumine. Peak concentrations of TxB2 in milk occurred at 8 hours after endotoxin inoculation. Flunixin meglumine treatment produced significant (P less than 0.05) reductions in milk TxB2 and plasma 15-keto-13,14-dihydro-PGF2 alpha concentrations. Concentrations of PGF2 alpha in milk and total PGF2 alpha and TxB2 production per quarter per milking were not significantly influenced by endotoxin challenge or by flunixin meglumine treatment.

Animals↗
PubMed1985

Use of tritium labeled amino acid conjugates of prostaglandins and thromboxanes as labeled ligands in prostanoid radioimmunoassay.

Conjugates of prostaglandins and thromboxanes with tritium labeled amino acids were prepared and employed as labeled ligands in prostaglandin and thromboxane radioimmunoassays. Assays for PGF2 alpha, 15-keto-13, 14-dihydro-PGF2 alpha, TXB2 and 15-keto-13,14-dihydro-TXB2 were evaluated in comparative studies using either these heterologous ligands or the corresponding homologous tritiated eicosanoid as tracers. Binding properties for the respective antibodies were found to be similar using either tracer. Three biological studies were also conducted, viz. study of the release of TXB2 during collagen induced platelet aggregation, of 15-keto-13,14-dihydro-TXB2 during guinea pig pulmonary anaphylaxis, and of PGF2 alpha (measured as 15-keto-13,14-dihydro-PGF2 alpha in peripheral plasma) during bovine luteolysis. The analyses gave comparable results using either the heterologous or the homologous assay. Thus, this type of labeled prostanoid conjugates may serve as a convenient alternative to homologous tracers in radioimmunoassay. Heterologous tracers may even in certain cases provide the only simple solution to the problem of preparing a labeled ligand of high specific activity.

Amino Acids↗
PubMed1985

Aggregation of and thromboxane B2 synthesis in platelets from newborn infants of smoking and non-smoking mothers.

Cord blood platelet aggregation and thromboxane B2 (TxB2) synthesis were investigated in 20 healthy infants of drug-free healthy mothers with an uncomplicated pregnancy ending at term in a normal vaginal delivery. Eight of the mothers were habitual smokers and the remaining 12 were non-smokers. The platelet count was slightly lower in infants of smoking mothers than in the control infants. There were no differences between the two groups in the qualitative or quantitative aggregation responses (maximal change in optical density) to stimulation by 0.55, 1.1, 3.3 and 6.6 microM adenosine diphosphate, 0.5 and 1.0 mM arachidonic acid (AA) or collagen 1.0 and 5.5 mg/l. Nor was there any difference between the groups in regard to TxB2 synthesis after stimulation by these substances. The amount of TxB2 synthesized merely reflected the occurrence of reversible or irreversible platelet aggregation. Stimulation of platelets by AA induced by far the highest synthesis of TxB2. The generally decreased aggregability of platelets of newborn infants, compared with that in adults, was confirmed in this study. No effect of maternal smoking during pregnancy on the prostaglandin-dependent pathway of aggregation of cord blood platelets was demonstrated.

Adult↗
PubMed1985

Studies on the endocrinology of parturition: relative steroidogenesis in coexisting genetically dissimilar ovine fetuses, concomitant with the temporal patterns of maternal C18 and C19 steroids and prostaglandin F2 alpha release.

Ovine embryos collected from two breeds of ewes possessing different gestational periods [Finnish Landrace (Finn), 145.5 +/- 1.4 days; Rambouillet (Ra), 150.7 +/- 1.3 days] were transferred to common, randomly selected recipients. Fetal plasma samples were collected from mixed-breed (MB, one Finn and one Ra; n = 6) and single-breed (SB, single Finn, n = 3; single Ra, n = 3) chronic fetal preparations during the last trimester. In the MB pregnancy, the Ra sibling had temporal patterns of androstenedione similar to those observed in the Finn co-twin; however, these particular changes were greatly accelerated in comparison to the same day of gestation in SB Ra fetuses. Similarly, temporal patterns of change in unconjugated estrone, estrone sulfate, and 17 beta-estradiol were accelerated by as much as 4.6 to 5 days in Ra fetuses of MB pregnancies when compared to Ra fetuses in the SB pregnancies (P less than 0.001), with no difference noted between Ra and Finn co-twins. Maternal progesterone concentrations changed from 8 to 2 ng/ml, androstenedione from 200 to 400 pg/ml, estrone from 65 to 250 pg/ml, and 15-keto-13,14-dihydroprostaglandin F2 alpha from 200 pg/ml to 6000 pg/ml over the last 4 or 5 days of gestation. Chronic fetal manipulation accelerated parturition, with mean gestational lengths in MB, SB Finn, and SB Ra pregnancies being 141.5 +/- 0.7, 141.7 +/- 0.7, and 149.3 +/- 0.9 days, respectively. In this study fetal endocrine changes were largely controlled in the MB pregnancy by the fetal co-twin exhibiting a shorter gestational period.

Androstenedione↗
PubMed1985

Arachidonic acid metabolites in milk of cows during acute coliform mastitis.

Concentrations of prostaglandin F2 alpha (PGF2 alpha) and thromboxane B2 (TXB2) were evaluated in the milk of cows with naturally occurring (n = 3) and experimentally induced (n = 5) acute coliform mastitis. These arachidonic acid metabolites were measured by radioimmunoassay in unextracted milk. Experimental infections were induced by inoculating 600 to 1,200 colony-forming units of Escherichia coli into 1 mammary quarter per experimental cow. In the experimental cows, milk was collected before inoculation and at 12, 24, 36, 48, 60, and 72 hours after inoculation. Somatic cell concentrations, bovine serum albumin, and concentrations of PGF2 alpha and TXB2 were determined in milk collected at each sampling. Mild-to-moderate increases in milk PGF2 alpha and TXB2 concentrations were observed in cows with naturally occurring mastitis. the increases corresponded to the clinical severity and course of mastitis. In the experimental cows, increases in milk PGF2 alpha and TXB2 concentrations were observed, but the increases were not significant, using a statistical model that included factors of treatment, cows, hours after inoculation, cows-by-treatment and hours-by-treatment interactions, and random error (residual). Results of the present study indicated a large biological variability in milk arachidonic acid metabolite concentrations in cows with acute coliform mastitis, and that arachidonic acid metabolites may be important in the pathophysiologic process of acute coliform mastitis.

Acute Disease↗