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Biomedical subjects

H Kikuchi

Publications and source records attributed to H Kikuchi.

At least 793 records · Page 44Linked to original sources
PubMed1985

[The antiinflammatory effect of budesonide ointment and cream].

The antiinflammatory effect of topically applied budesonide ointment on carrageenin induced paw edema in rats, croton oil induced ear edema in rats, passive cutaneous anaphylaxis (PCA) in rats and picrylchloride induced contact hypersensitivity in mice was studied and compared with those of commercially available ointments containing betamethasone 17-valerate, hydrocortisone 21-acetate, hydrocortisone 17-butyrate or fluocinonide. The five ointments had almost the same degree of activity against the carrageenin induced paw edema. Budesonide ointment was strongest in inhibiting the croton oil induced ear edema. Budesonide and fluocinonide ointments were stronger than the other 3 ointments in inhibiting PCA and picrylchloride induced hypersensitivity. No clear atrophic effect on the thymus or adrenal was observed with any of the ointments at the doses tested. When the effect of budesonide ointment was compared with that of budesonide cream, there were no differences in activity between the two formulations. The results suggest that budesonide is a useful drug with a superior topical antiinflammatory activity.

Administration, Topical↗
PubMed1985

[The anti-inflammatory effect of auranofin].

The anti-inflammatory effects of auranofin were studied and compared with those of indomethacin, gold sodium thiomalate (GST) and D-penicillamine. Auranofin was active as indomethacin in inhibiting carrageenan induced paw edema in rats, but was less potent than indomethacin in inhibiting UV-induced erythema in guinea pigs. Auranofin inhibited Arthus type paw edema and reverse PCA reaction in rats, on which indomethacin was ineffective. The inhibitory activity of auranofin on adjuvant arthritis was weaker than that of indomethacin. In in vitro experiments, auranofin did not show any suppression of cyclooxygenase activity, but was capable of suppression of lysosomal enzyme release and chemotaxis of neutrophils and macrophages. In addition to these anti-inflammatory activities, auranofin had almost equal anti-analgesic and anti-pyretic activity to that of indomethacin. The above results indicated that the anti-inflammatory profiles of auranofin and indomethacin differ, so we can expect new therapeutic activities of auranofin. GST had similar anti-inflammatory and anti-analgesic profiles to those of auranofin; however, the activities were less potent than auranofin and devoid of anti-pyretic activity. D-penicillamine did not show any anti-inflammatory, anti-analgesic or anti-pyretic activity.

Animals↗
PubMed1985

The hypotensive effect of 2-(5-chloro-2-phenoxyanilino)-2-imidazoline (FR35447).

The hypotensive effect of FR35447 was comparable to that of prazosin and was more potent than that of hydralazine, but its duration of action was shorter. Repeated administration of FR35447 or prazosin to hypertensive rats for 5 consecutive days induced no significant difference in the intensity or duration of the hypotensive effect. In contrast, marked tachyphylaxis to hydralazine or phentolamine was observed. FR35447 as well as prazosin induced only a transient increase in cardiac output in anesthetized dogs, whereas hydralazine induced a longlasting increase. This difference may contribute to no development of tolerance to FR35447 or prazosin. FR35447 decreased the pressor response to noradrenaline, but not that to angiotensin II or vasopressin in pithed rats, which indicates that FR35447 is an alpha-adrenoceptor antagonist. FR35447 has some selectivity for alpha 2-adrenoceptors, but the selectivity was far less than that of yohimbine. Since FR35447 induced only slight hypotension following intracerebroventricular injection in anesthetized cats, the hypotensive effect of the drug does not appear to be mediated through the central nervous system. Whereas prazosin induced a dose-dependent increase in blood glucose in rats, FR35447 showed no significant effect.

Animals↗
PubMed1985

Immunoactive peptides, FK 156 and FK 565. IV. Activation of mouse macrophages.

We investigated the effects of the immunoactive peptides, FK 156 and FK 565, on functions of mouse macrophages. FK 156 and FK 565 given parenterally or orally to mice enhanced spreading of peritoneal macrophages, phagocytosis of latex particles and intracellular killing of bacteria by peritoneal macrophages. FK 156 and FK 565 also enhanced the production of superoxide anion and lysosomal enzyme activities of macrophages. The peptides also activated mouse spleen macrophages, and the kinetics of this activation differed from that of the peritoneal macrophages. In addition, both drugs directly enhanced the production of superoxide anion by mouse peritoneal macrophages treated in vitro and enhanced the functions of peritoneal macrophages of athymic nude mice. Both these phenomena suggest that direct activation might be one of the mechanisms of macrophage activation by the peptides.

Adjuvants, Immunologic↗
PubMed1985

[On the time of cardiac aneurysm formation following acute myocardial infarction].

It has been said that ventricular aneurysm is formed in the relatively late stage after the onset of acute myocardial infarction. We examined the time of its formation using digital subtraction angiography (DSA) performed immediately after infarction and at various intervals thereafter. We also examined correlations between aneurysm formation and the degree of rest after infarction, blood pressures, sites of infarction and coronary angiographic findings. The subjects consisted of 35 hospitalized patients with acute myocardial infarction. They were examined by DSA immediately, and one week and one month after their admissions. DSA was performed in the 30 degree right anterior oblique projection, and cardiac aneurysms were diagnosed by the presence of regional protrusion or of dyskinesis of the left ventricular wall on left ventriculography. The results were as follows: Cardiac aneurysms were noted in eight men and four women. The mean age was 69.2 +/- 8.1 years. Infarctions were located in the anteroseptal region (nine patients), in the broad anterior wall (two patients) and in the inferior wall (one patient). The average onset-to-admission interval was 5.6 hours in the aneurysm group, and eight hours in the aneurysm-free group. Cardiac aneurysms were demonstrated by DSA immediately after hospital admission in all 12 patients in the aneurysm group and the size did not increase appreciably with time. The peak CPK was significantly higher in the aneurysm group (3,163) than in the aneurysm-free group (1,655), but there was no group-related difference in risk factors, hypertension, the duration of rest after infarction, or coronary angiographic manifestations. Cardiac aneurysm has been considered as a late complication of myocardial infarction. Many investigators have reported that its formation begins one to four weeks after the onset of infarction with gradual protrusion. In the present study, however, the formation of aneurysms was complete at very early stages after the onset of the myocardial infarction and often encountered in patients with relatively extensive infarction.

Aged↗
PubMed1985

[Effect of intravenous administration of FT-207 for gastric cancer].

FT-207, 800mg per day, was administered intravenously 2 hours per day for 6 days to 15 patients with gastric cancer. By chemical assay, FT-207 and 5-FU concentrations in the blood and tissues were determined. The FT-207 levels in cancerous tissue, metastatic lymph nodes and normal gastric mucosa were almost equal. The mean 5-FU level in cancerous lesions was 0.110 +/- 0.075mcg/g, and was 0.124 +/- 0.080mcg/g in lymph nodes, and 0.043 +/- 0.021mcg/g in normal mucosa. This showed that 5-FU levels were significantly higher in tumors and lymph nodes than in normal mucosa. (p less than 0.05, p less than 0.01 respectively). The mean blood level of 5-FU was low at 4 hours after FT-207 infusion. In conclusion, intravenous drip administration of FT-207 was considered to be effective for gastric cancer because of the high tumor affinity of 5-FU.

Drug Administration Schedule↗
PubMed1985

[Study of adult cases of moyamoya disease].

Fifty-one adult patients with moyamoya disease were studied. Among these, thirteen cases, which had the history of cerebral ischemic attack during childhood, are classified as the cases of juvenile onset. The other thirty-eight cases were classified as those of adult onset. In most of the cases of juvenile onset, occlusive lesions also affected cortical arteries. For these cases, it is important to perform STA-MCA double anastomoses and encephalo-myo-synangiosis. Those of adult onset resembled the pathophysiology of internal carotid artery occlusion, or that of middle cerebral artery occlusion, since few cortical artery occlusion was noted, and leptomeningeal anastomosis was well developed. STA-MCA anastomosis was considered to be the treatment of choice in those of adult onset.

Adolescent↗
PubMed1985

[Heterogeneity of a KU-2 cell line derived from human renal cell carcinoma--differential susceptibilities of its sublines to NK cell-mediated cytotoxicity].

Natural killer (NK) cell activity against 4 different sublines of a permanent cell line KU-2 derived from human renal cell carcinoma were measured by 51Cr-release assay and single cell binding assay. KU-2 and its sublines exhibited different susceptibilities of interferon (IFN)-augmented NK cell cytotoxicity. Moreover, 2 out of 4 sublines changed in susceptibility to NK cell cytotoxicity in a few months. Along with the direct assay for NK cell activity, frequency and kinetics of NK cell-mediated cytotoxicity against 3 sublines were studied by single cell binding assay where spontaneous killing of target cells was directly visualized by the reading of trypan blue uptake into target cells among effector-target cell conjugates. The frequency of killer cells among conjugates was dependent on the susceptibility of the sublines to the IFN-augmented NK cell cytotoxicity. These results suggested the following: KU-2 cell line was not homogeneous but composed of a heterogeneous population of cells and even in one subline derived from a clone, the cells with different susceptibilities to NK cell-mediated cytotoxicity developed spontaneously in ordinary culture conditions with the passage of a certain period of time.

Carcinoma, Renal Cell↗
PubMed1984

In vivo spin-trap study on anaerobic dehalogenation of halothane.

Radical formation in vivo by anaerobic dehalogenation of halothane is described in this paper. The radicals were stabilized by spin-trapping and assayed by electron spin resonance spectrometry. The radical adducts were formed by inhalation of halothane in vivo and increased with decrease in inspired oxygen concentration. Following administration of the spin-trap, the expired concentration of CF2CHCl and CF3CH2Cl which are the anaerobic metabolites of halothane decreased, but bilious trifluoroacetate which are aerobic did not change. These results strongly suggest that radical intermediates are produced in anaerobic dehalogenation of halothane to CF2CHCl and CF3CH2Cl.

Anaerobiosis↗