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Biomedical subjects

H Kida

Publications and source records attributed to H Kida.

At least 235 records · Page 13Linked to original sources
PubMed1986

[Immunological response of regional lymph nodes after cryosurgery in rats].

At 7 days after MRMT-1 inoculation at the thigh of 5 week-old female SD rats, the tumor was treated cryosurgically by 2 cycle freezing, contact method at -170 degrees C. Weights of thymus and the spleen, histological findings of lumbar lymph nodes, PHA-induced blastogenesis of lymphocytes obtained from lumbar lymph nodes or peripheral blood, and resistance rate to tumor re-challenge were examined at 1, 3, 6, 10 and 17 week(s) after cryosurgery. Following results were obtained. (1) Weight of the thymus gradually decreased by 3 weeks after cryosurgery, while that of the spleen increased by 1 week, recovering the preoperative level at 7 weeks. (2) Paracortical hyperplasia of lumbar lymph nodes markedly increased at 1 week and sinus histiocytosis increased after 3 weeks, both keeping their high values till 10 weeks, while germinal center hyperplasia showed a high value at 2 weeks, thereafter decreasing gradually. (3) PHA-induced blastogenesis of lumbar lymph nodes significantly increased at 1 week, keeping its high value till 10 weeks. (4) PHA-induced blastogenesis of peripheral lymphocytes showed the lowest value at 3 weeks, then significantly increasing at 6 weeks. (5) Resistance rate to re-challenge showed the lowest value at 3 week, reading the highest level at 10 weeks after cryosurgery. From the above results, it was suggested that anti-tumor immunity induced by cryosurgery was at the lowest level at 3 weeks, and gradually increased starting at 6 weeks. Augmentation of helper T cell and cytotoxic T cell functions at the regional lymph nodes was also suggested to occur from 1 week to 10 weeks after cryosurgery.

Animals↗
PubMed1986

Participation of extracapillary lesions (ECL) in progression of IgA nephropathy.

To clarify long-term prognosis and risk factors of IgA nephropathy, 205 patients with the disease were followed up for a period of 1 to 22 years with a mean of 7.9 years. According to the percentage of glomeruli involved with extracapillary lesions (ECL), which were defined by crescents and fibrous adhesion of glomerular tufts to Bowman's capsule, the patients were divided into four groups: group 1-absence of ECL; group 2-less than 25%; group 3-25-50%; group 4-more than 50%. During the follow-up period, 26 patients progressed to chronic renal failure requiring hemodialysis, and one patient died of acute peritonitis. The actuarial kidney survival rate was 90.4% for 5 years and 86.0% for 10 years. Ten-year survival rates were 100% in group 1, 94.3% in group 2, 81.8% in group 3 and 25.5% in group 4. Re-biopsy specimens, observed in 31 patients after intervals of 1.4 to 13.4 years, revealed an increase of ECL in 20 patients concomitant with an apparent reduction of renal function in 13, whereas in the other 11 patients with no increase, renal function remained unchanged. These results strongly suggest that ECL plays an important role in the progression of IgA nephropathy, and renal function could be impaired by repeated formation and accumulation of these lesions.

Adolescent↗
PubMed1985

Antitumor effect in peritoneal carcinomatosis of intraperitoneal administration of a streptococcal preparation, OK-432: I. Experimental study in the rat.

The effect of intraperitoneal OK-432 administration and its mechanism of action were investigated using an experimental rat tumor. On day 4 or 6 of intraperitoneal inoculation of tumor cells, 50 Klinische Einheit (KE)/kg of OK-432 was intraperitoneally administered, and intraperitoneal exudate cells were microscopically examined daily. Distribution of peritoneal exudate cells: A marked increase in neutrophils was noted in ascites at 24 hours after intraperitoneal administration of OK-432, with macrophages and lymphocytes appearing later than neutrophils. A subsequent decrease in neutrophils was accompanied by a gradual increase in tumor cells. Findings of tumor cells: When OK-432 was intraperitoneally administered on day 4 of tumor inoculation, aggregation of neutrophils around tumor cells was observed on day 6, and rosette formations around tumor cells were noted on day 7 or 8. Subsequently tumor cells that formed the centers of the rosettes were destroyed and disappeared. These reactions were not observed when OK-432 was administered on day 6, nor were they observed in untreated cases. These results suggest that cytotoxic effects of intraperitoneally administered OK-432 were displayed mainly by rosette formation by neutrophilic cells, as far as this model was concerned.

Animals↗
PubMed1985

The effect of furosemide on glucose oxidation of the cochlea and other tissues.

We tested the hypothesis that furosemide interferes with energy generation in the cochlea, and determined its effect on CO2 formation from glucose and glyceroaldehyde-3-phosphate dehydrogenase (GAPDH) activity by examining biochemical and histochemical changes in the cochlea, the kidney, and the liver. We found that furosemide suppressed CO2 formation in vitro at relatively low concentrations in all tissues examined. GAPDH was inhibited as soon as 2 min after its administration (80 mg/kg, i.v.). Recovery of this enzyme activity was most rapid in the liver. We concluded that furosemide does interfere with energy generation in the cochlea, kidney, and liver as a result of its inhibition of GAPDH.

Animals↗
PubMed1985

Is bivalent binding of monoclonal antibodies to different antigenic areas on the hemagglutinin of influenza virus required for neutralization of viral infectivity?

Biological activities of Fab fragments of monoclonal IgG antibodies to each of four nonoverlapping antigenic areas on the hemagglutinin molecule of A/seal/Massachusetts/1/80 (H7N7) influenza virus were examined. Fab fragments of the antibodies belonging to groups I and II neutralized viral infectivity. These Fab fragments inhibited hemagglutination of the virus and virus-induced hemolysis at pH 5.9. On the other hand, Fab fragments of groups III and IV antibodies showed neither neutralization nor hemolysis-inhibition activities, while intact IgG molecules of groups III and IV effectively neutralized viral infectivity and inhibited virus-induced hemolysis, as previously found. These IgG molecules scarcely or did not inhibit hemagglutination of the virus. Neutralization of viral infectivity, however, was observed when the virus was coated with Fab fragments of groups III and IV antibodies and then incubated with anti-Fab fragment antibodies. These findings suggest that bivalent binding of the IgG antibodies of groups III and IV is required for neutralization of viral infectivity through a proposed mechanism by which these antibodies interfere with a low pH-induced conformational change resulting in inhibition of the fusion step of the viral replication process.

Antibodies, Monoclonal↗
PubMed1985

Antigenic analysis of H4 influenza virus isolates using monoclonal antibodies to defined antigenic sites on the hemagglutinin of A/Budgerigar/Hokkaido/1/77 strain.

Three non-overlapping antigenic sites were defined on the hemagglutinin of avian influenza virus A/budgerigar/Hokkaido/1/77 (H4N6) by competitive binding assay of monoclonal antibodies to the virus and comparative antigenic analysis of variants selected with monoclonal antibodies. Antigenic relationship among 25 H4 influenza viruses of different bird origin was examined by ELISA with the monoclonal antibodies to each of defined antigenic sites. Two of the three antigenic sites contained epitopes specific to the H4 influenza viruses of budgerigar and mynah origin, and the remaining site contained an epitope which was cross-reactive with almost all of the H4 influenza viruses.

Animals↗
PubMed1985

Inhibition of transcriptase activity of influenza A virus in vitro by anti-haemagglutinin antibodies.

An investigation was made of inhibition of transcriptase activity of influenza viruses in vitro by binding of antibody to the surface of the virion. Eight monoclonal antibodies which were directed against at least four non-overlapping antigenic regions of the haemagglutinin protein of A/Aichi/68 virus were tested for inhibitory effect. One of the antibodies directed against the B antigenic site, 22/1, inhibited transcriptase activity, while the other seven antibodies did not. Antibody from a hyperimmune rabbit serum to A/Udorn/72 (H3N2) virions inhibited the transcriptase activity of A/Udorn/72 and A/Aichi/68 (H3N2) viruses but not that of A/WSN/33 (H1N1). The antibody did not cause irreversible inactivation of the transcriptase since full activity was recovered by isolating ribonucleoprotein (RNP) cores from the inhibited virions using NP-40 treatment and subsequent centrifugation in a caesium sulphate density gradient. The antibody did not inhibit transcriptase activity of isolated RNP cores. The virion transcriptase activity was not inhibited by addition of the antiserum after the detergent treatment which is necessary for the activation of the transcriptase activity in vitro. These results suggest that the antibody blocks the activation process of the transcriptase by detergent treatment.

Antibodies, Viral↗
PubMed1985

Interference with a conformational change in the haemagglutinin molecule of influenza virus by antibodies as a possible neutralization mechanism.

A possible mechanism of neutralization of influenza virus by antibodies to the haemagglutinin molecule is proposed in addition to the generally accepted mechanism of blocking attachment to host cell receptors. This proposed mechanism involves interference with a low-pH-induced conformational change in the haemagglutinin molecule by bivalent binding of antibodies, which results in inhibition of the fusion step in the viral replication process.

Antibodies, Monoclonal↗
PubMed1985

Survival of Corynebacterium renale, Corynebacterium pilosum and Corynebacterium cystitidis in soil.

Survival of the causative agents of bovine pyelonephritis, Corynebacterium renale, C. pilosum and C. cystitidis, was examined at 30 degrees C in autoclaved soil. In the soil from a paddock, C. renale and C. cystitidis survived for 56 and 63 days, respectively, and C. pilosum for a longer period of at least 210 days. In soil from a pasture, sand from an athletic field and sea sand, the survival of these bacteria was of shorter duration.

Animals↗
PubMed1985

Adhesion of Corynebacterium renale and Corynebacterium pilosum to the epithelial cells of various parts of the bovine urinary tract from the renal pelvis to vulva.

The various parts of the bovine urinary tract, the renal pelvis, the ureter, the urinary bladder, the urethra, the vaginal vestibule and the vulva, were examined for the capacity of the epithelial cells to bind Corynebacterium renale and C. pilosum. C. renale adhered best to the epithelial cells of the vulva, and then to those of the ureter and renal pelvis. C. pilosum also adhered best to the epithelial cells of the vulva, followed by those of the vaginal vestibule. The results indicate that the most important target tissue for these bacteria may be the vulva, and the results correlate with the fact that C. renale frequently causes pyelonephritis and ureteritis, while C. pilosum causes the same diseases less frequently and behaves like normal flora of the vaginal vestibule.

Adhesiveness↗
PubMed1985

Characterization of a partially purified leptospiral genus-specific protein antigen.

A leptospiral genus-specific protein antigen (GP-Ag) was partially purified from Leptospira interrogans serovar kremastos strain Kyoto and canicola strain Hond Utrecht IV, by treating the organisms with Triton X-100 followed by a purification procedure including fractionation with DEAE-cellulose column chromatography and ethanol precipitation. Genus-specificity of the antigens from kremastos Kyoto and canicola was shown by the immunodiffusion (ID) test. Complement fixation (CF) test and enzyme-linked immunosorbent assay (ELISA) using GP-Ag from kremastos Kyoto showed the genus specificity of the GP-Ag. Sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) of the antigens from kremastos Kyoto and canicola showed a major band with Coomassie blue stain. The band of GP-Ag from kremastos Kyoto was also shown by immunoprecipitation using the monoclonal antibody (GP-7) to GP-Ag from kremastos Kyoto. The molecular weight of the genus-specific antigen was estimated to be approximately 62 000. The genus specificity of the antigen was reduced by heating at 100 degrees C or treatment with proteolytic enzymes, but not reduced by treatment with sodium metaperiodate.

Agglutination Tests↗
PubMed1985

Detection of antibodies to leptospiral genus-specific antigen in human and animal sera by indirect hemagglutination test with a partially purified genus-specific protein antigen.

Antibodies against leptospiral genus-specific antigen were detected in the sera from clinically diagnosed human leptospirosis and suspected animal leptospirosis by indirect hemagglutination (IHA) test with a partially purified genus-specific protein antigen (GP-Ag). The reaction was positive in the infected humans and animals irrespective of the leptospiral serovars. No significant correlation was found between IHA titer against GP-Ag and microscopic agglutination (MA) titer. IHA titer did not always develop in parallel with MA titers. Sera obtained from healthy individuals were negative in both IHA and MA tests.

Animals↗
PubMed1985

Production of monoclonal antibodies against leptospiral genus-specific protein antigen and localization of the antigen by immunoelectron microscopy.

Monoclonal antibodies were first produced against a leptospiral genus-specific protein antigen (GP-Ag) isolated from Leptospira interrogans serovar kremastos strain Kyoto. The representative monoclonal antibody (GP-7) did not agglutinate kremastos Kyoto but reacted with Triton X-100 treated kreamastos Kyoto, andamana, patoc and Urawa in enzyme-linked immunosorbent assay (ELISA), indicating that the monoclonal antibody recognized the genus-specific determinant of GP-Ag. The localization of GP-Ag was investigated by immunoelectron microscopy with the iodinated antibody and peroxidase-labeled antibody. The genus-specific determinant of GP-Ag recognized by GP-7 was located on the subsurface of the leptospiral envelope.

Animals↗
PubMed1985

[Clinical study on serum immunosuppressive factors in gastric cancer patients--with special reference to preoperative IAP values and the influence of surgical stress].

Serum IAP values were measured preoperatively in 83 gastric cancer patients. The levels were significantly higher in cases of total, stage III and IV, n (+) and non-curative resection than in normal subjects, stage I, n (-) and curative resection. There was a significant correlation between IAP on the one hand, and IgG FcR (+) T cell, alpha 2-globulin and CEA on the other. OK-432 was serially administered pre- and postoperatively (A) and only postoperatively (B). IAP was examined at one, two and four weeks postoperatively, and the findings were compared. It was increased markedly at one week and was slightly reduced at two and four weeks. These changes in IAP showed no significant differences between groups A and B.

Carcinoembryonic Antigen↗
PubMed1985

Adhesion of Corynebacterium pilosum by pili to epithelial cells of bovine vulva.

Piliated (P+) and nonpiliated (P-) clones of Corynebacterium pilosum were selected, and their adhesion to the epithelial cells of the bovine vulva and vaginal vestibule was examined. The number of P+ bacteria of C pilosum that adhered to vulval epithelial cells was greater than that of P- bacteria. The adhesion of P+ bacteria, but not P- bacteria, to the epithelial cells was inhibited by the antipilus antiserum; therefore, the adhesion of C pilosum to the epithelial cells of the vulva was primarily dependent on the pili. The number of C pilosum that adhered to the epithelial cells of the bovine vulva and of the vaginal vestibule increased by decreasing the pH.

Adhesiveness↗