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Biomedical subjects

H Kida

Publications and source records attributed to H Kida.

At least 217 records · Page 12Linked to original sources
PubMed1987

[Experience with CDDP therapy in advanced and recurrent gastric cancer cases].

For 3 years beginning in 1984, 14 advanced and recurrent gastric cancer cases with measurable lesions (8 unresected cases, 3 cases of recurrence, 2 cases with non-curative resection, 1 case of no operation) were treated by CDDP therapy alone or in combination with other chemotherapies. CR was obtained in 2 of the 8 non-resected cases, radical gastrectomy being feasible in these 2 cases by second-look operation. Five of the 8 cases showed PD, and in 1 case the effect was rather obscure. One of the 2 non-curative resection cases achieved PR, and the other case was MR. One of the 3 recurrent cases was NC, the remaining cases being PD. The one case given no operation showed PD. Overall effectiveness of CDDP therapy for advanced and recurrent gastric cancer cases was seen in 3 (21.4%) of the 14 cases. All of these 3 cases showing effective CDDP therapy was elevated serum CEA values before the therapy, which was markedly reduced after starting the therapy, the reduction rate being very remarkable.

Adult↗
PubMed1987

Impaired immunoglobulin G production in minimal change nephrotic syndrome in adults.

Serum IgG, T and B cell subsets, cytoplasmic IgG positive cells (cB gamma) and IgG in the medium (cIgG) of a 5 day culture of peripheral lymphocytes in both stimulated and non-stimulated (spontaneous) conditions with pokeweed mitogen (PWM) were studied in 30 adult patients with minimal change nephrotic syndrome (MCNS). In the nephrotic phase (11 patients), surface IgG positive B cells (sB gamma) and spontaneous cB gamma increased (P less than 0.05), whereas PWM-stimulated cIgG did not increase, and serum IgG decreased significantly (P less than 0.05). The cB gamma/sB gamma ratio calculated as an index of IgG synthesis in B cells increased spontaneously (P less than 0.05), but did not increase under PWM-stimulation. The cIgG/cB gamma ratio as an index of IgG secretion from each matured B cell, reduced in both spontaneous and stimulated conditions (P less than 0.05, P less than 0.01, respectively). In the phase of unstable remission maintained by steroid therapy (10 patients), these parameters tended to normalize and the OKT4/OKT8 ratio decreased (P less than 0.05), while the ratio remained unchanged in the nephrotic phase. However, after discontinuation of steroid (nine patients), spontaneous cB gamma and the spontaneous cB gamma/sB gamma ratio were again increased, and the cIgG/cB gamma ratio decreased (P less than 0.05) as observed in the nephrotic phase. These results suggest that B cells in patients with MCNS both in the nephrotic state and stable remission after discontinuation of steroid are activated spontaneously, but the secretory process of IgG from the matured cells is impaired, and that steroid improves these abnormalities.

Adolescent↗
PubMed1986

Clinical studies on serum immunosuppressive acidic protein (IAP) and ferritin in gastric cancer patients: with special reference to preoperative value and influence of surgical stress.

Serum IAP and ferritin values were measured preoperatively in 83 resectable gastric cancer patients. IAP was significantly higher in them than in normal subjects, and increased gradually with progression of cancer, and the values at stages III and IV were significantly higher than that at stage I. It was significantly higher in the cases of n(+) than in those of n(-), and in the cases of noncurative resection than in those of curative resection. Ferritin was significantly higher in them than in normal subjects and mammary cancer patients, and in males than in females, and the values at stages I and IV were significantly higher than that at stage III. OK-432 was serially administered pre- and postoperatively in 83 cases and only postoperatively in 33 cases. IAP increased markedly at 1 week postoperatively and was reduced gradually at 2 and 4 weeks. Ferritin increased markedly at 1 week after operation and reached the maximum value at 2 weeks and was reduced toward the value of 1 week at 4 weeks. However, these changes showed no significant difference between the both groups.

Breast Neoplasms↗
PubMed1986

Antigenic mapping and functional analysis of the F protein of Newcastle disease virus using monoclonal antibodies.

Twelve monoclonal antibodies to the F protein of a velogenic strain of Newcastle disease virus (NDV) were established. Each of these antibodies inhibited virus-induced plaque formation in BHK-21 cells. Seven antibodies neutralized viral infectivity in eggs; thus, antigenic variants could be selected with these antibodies and used for antigenic mapping. Based on the reactivity of the antigenic variants with the antibodies used in selection, 4 distinct antigenic sites (I-IV) were defined on the F protein molecule. In competitive binding assay, sites II and III were found to be spatially close to each other. Each antibody to sites I, II and III inhibited both virus-induced hemolysis of chicken erythrocytes and syncytium formation of BHK-21 cells. On the other hand, some of the antibodies to site IV selectively inhibited either hemolysis or cell fusion. This finding may indicate that the fusion of the viral envelope with erythrocytes and host cell membrane is modulated through different ways. Comparative analysis of different NDV strains using monoclonal antibodies to each of the different antigenic sites showed that the antigenicity of the F protein is highly conserved.

Animals↗
PubMed1986

Immunological response of regional lymph nodes after tumor cryosurgery: experimental study in rats.

Seven days after inoculation of metastasizing rat mammary tumor No. 1 into the thigh of 5-week-old female Sprague-Dawley rats, the tumor was treated cryosurgically by two-cycle freezing and by contact methods at -170 degrees C. Weights of the thymus and the spleen, histological findings of the lumbar lymph nodes, phytohemagglutinin (PHA)-induced blastogenesis of lymphocytes obtained from the lumbar lymph nodes and peripheral blood, and resistance rate to tumor rechallenge were examined 1, 3, 6, 10, and 17 week(s) after cryosurgery, with the following results: Thymus weight gradually decreased by 3 weeks after cryosurgery, while spleen weight increased by 1 week, recovering the preoperative level at 6 weeks. Paracortical hyperplasia of the lumbar lymph nodes markedly increased in 1 week and sinus histiocytosis increased after 3 weeks, both remaining at high values until 10 weeks, while germinal center hyperplasia showed a high value at 3 weeks and thereafter decreased gradually. PHA-induced blastogenesis of the lumbar lymph nodes significantly increased 1 week after cryosurgery and remained at its high value until 10 weeks. PHA-induced blastogenesis of peripheral lymphocytes showed the lowest value at 3 weeks and then significantly increased at 6 weeks. Resistance rate to rechallenge showed the lowest value at 3 weeks, reaching the highest level 10 weeks after cryosurgery. From the above results, it was suggested that anti-tumor immunity (resistance to tumor rechallenge) induced by cryosurgery was at the lowest level at 3 weeks after cryosurgery, and gradually increased starting at 6 weeks.(ABSTRACT TRUNCATED AT 250 WORDS)

Animals↗
PubMed1986

Spin-labeling of influenza virus hemagglutinin permits analysis of the conformational change at low pH and its inhibition by antibody.

To study the conformational changes in the hemagglutinin (HA) molecule of A/seal/Mass/1/80 (H7N7) (Seal) influenza virus at low pH, a spin-labeling method was used. This method also permits study of antibody interaction with the HA. A synthetic nitroxide compound was used for spin-labeling of tyrosine residues of the isolated HA molecule. Electron spin resonance (ESR) spectra of the spin-labeled HA at various pH values indicated that a conformational transition occurred under acidic conditions, and around pH 5.8 the HA molecule has maximal flexibility. Since virus-induced hemolysis occurs optimally at pH 5.8-5.9, the HA molecule in the maximally flexible conformation is considered to mediate membrane fusion. The ESR spectra of the antibody-bound HA at various pH values revealed that monoclonal antibodies to different regions on the molecule may inhibit the conformational change by different modes. One antibody inhibited the changes in the HA that resulted in flexibility, while the other did not. These results support the assumption that monoclonal antibodies, which failed to inhibit hemagglutination of the virus yet neutralized viral infectivity, inhibited the fusion step in the viral replication process by interfering with a low pH-induced conformational change in the HA molecule (Kida, H., Webster, R.G. and Yanagawa, R. (1983) Arch. Virol. 76, 91-99).

Animals↗
PubMed1986

Gouty kidney associated with membranous nephropathy: participation of renal tubular epithelial antigen.

Histological studies were performed on 3 patients with gout and proteinuria measured at 1.0 g a day or more. Light microscopy revealed diffuse thickening of the glomerular capillary walls accompanied by spike formation and bubble-like appearance as well as tophaceous granuloma in the interstitium, tubular atrophy and benign nephrosclerosis. Immunofluorescence technique showed fine granular deposits of IgG and C3 along the glomerular capillary walls together with the renal tubular epithelial antigen (RTE) in 1 patient. Subepithelial dense deposits were also observed by electron microscopy. These findings suggest that the association of membranous nephropathy should be considered in patients with gout having moderate to severe proteinuria and that RTE may be involved in the pathogenesis of subepithelial deposits in gouty membranous nephropathy.

Adolescent↗
PubMed1986

[Cytotoxicity of interleukin 2-induced lymphocytes and effects of serum immunosuppressive factors].

IL2-induced lymphocytes (IIL) obtained from normal subjects were examined as to a possibility for induction of tumor-specific killer cells. Moreover, effects of serum on their cytotoxic activity were observed. The IIL which were pre-sensitized with mitomycin C-treated MKN-28 (IILM), showed a markedly increased cytotoxic activity against MKN-28. The IIL which were either pre-cultured with PHA or not, showed augmentation of cytotoxic activity against various human cancer cell lines, although their cytotoxic activity against MKN-28 was lower than that of IILM. The cytotoxic activity of IILM was suppressed by addition to the medium of non-specific immunosuppressive factors (IAP, ferritin, alpha-fetoprotein) or of various sera obtained from gastric cancer patients, in whom histological diagnosis was tub1, por or sig, but the suppression to the IILM activity was significantly milder than that to the activity of peripheral lymphocytes. The degree of suppression by tub1 serum was significantly higher than that by the serum from por or sig. When tub1 serum was added to the medium during pre-sensitization, the cytotoxic activity of IILM against MKN-28 was reduced significantly. However, addition of por or sig serum showed no significant reduction in cytotoxic activity of IILM.

Adenocarcinoma↗
PubMed1986

[Ultrasonic diagnosis of early gallbladder cancer].

In a series of 514 consecutive cholecystectomy specimens excised for biliary disease, 1.4% exhibited early carcinoma and 4.5% advanced. On the other hand benign tumors and pseudotumors were observed in 2.0% of cholesterol polyps, 0.8% adenomas, 1.0% inflammatory polyps and 10.8% adenomyomatosis. On the basis of these finding in this series, diagnostic usefulness and difficulties of ultrasound were discussed. Sonography can provide critical diagnostic information for preoperative identification of small polypoid lesion and irregular wall thickness.

Adenocarcinoma↗
PubMed1986

Long-term prognosis of membranous nephropathy.

One hundred and four consecutive patients with idiopathic membranous nephropathy have been followed for 24 years. The actuarial survival rates in 5, 10 and 15 years were 94.6, 90.0 and 80.3%, respectively. Within the first 5 years clinical status improved gradually, but thereafter entered into an equilibrated state, when approximately 70% of the patients were in remission of absent (40%) or less than 1.0 g/24 hour urinary protein. As for renal function, within the initial 5 years around 80% of the patients revealed a glomerular filtration rate (GFR) greater than 80 ml/min. This percentage reduced approximately to 70 and 50% over periods of 10 and 15 years, whereas a mean reduction rate of GFR was only 15 ml/min over a period of 8 years. The clinical status at the 5th year correlated well with its outcome (rs = 0.676, p less than 0.001) and GFR estimated around the 5th year with the final estimate (r = 0.796, p less than 0.001). These data suggest that membranous nephropathy follows a fairly good clinical course, and that the 5th year clinical status and renal function are valuable prognostic indices of the disease.

Adolescent↗