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Biomedical subjects

H Kemona

Publications and source records attributed to H Kemona.

At least 55 records · Page 3Linked to original sources
PubMed1988

Serum antithrombin III and alpha-2-antiplasmin concentration in patients with lung carcinoma.

Serum antithrombin III and alpha-2-plasmin inhibitor concentrations has been evaluated in 26 patients with lung carcinoma. We observed a twofold decrease in antithrombin III level and no differences between test and control groups in alpha-2-plasmin inhibitor concentrations. Evidently, the decreased antithrombin III level may reflect its consumption because of enhanced plasma thrombin activity, whereas normal alpha-2-plasmin inhibitor may result in no induction of secondary fibrinolysis followed by the stimulation of the coagulation system or no conditions for primary increased fibrinolysis. It seems possible, that the antithrombin III level in the serum may at least partly reflect the tendency for hypercoagulability and spreading of cancer.

Adenocarcinoma↗
PubMed1988

The distribution of peroxidase in phagocytizing human blood platelets, electron microscopy findings.

On the basis of a previously elaborated method for testing the phagocytic ability of human blood platelets we have undertaken studies for testing the distribution of peroxidase in phagocytizing platelets. Our findings confirmed the distribution of peroxidase in the dense tubular system and in granules in regular blood platelets. In the course of phagocytosis we noted the presence of peroxidase less evidently in both subcellular structures during the first of 5 minutes. In the next steps we observed the localization of this enzyme mainly near the phagolysosome. The change of this localization and the role of this enzyme in unspecific immunity against bacteria and neoplasia is briefly discussed.

Blood Platelets↗
PubMed1988

Serum concentration of alpha-2-macroglobulin, alpha-1-antitrypsin and alpha-2-antichymotrypsin in patients with lung carcinoma.

Serum concentration of alpha-2-macroglobulin, alpha-1-antitrypsin and alpha-2-antichymotrypsin was evaluated in 26 patients with lung carcinoma. We observed an evident decrease in alpha-2-M and alpha-1-antitrypsin level and no differences between tested and control groups in alpha-1-antichymotrypsin concentration. The deficiency of protease inhibitors may be due to the increased level of protease activity in malignant cells. Infiltration of granulocytes near tumor and released enzymes from them may exhaust proteolytic inhibitory capacity, too. Increased protease activity is associated with transformation and uncontrolled proliferation, therefore antiproteases may be accepted as anticancerogenic factors. Further investigations are needed to bring us closer to understanding this question.

Adult↗
PubMed1987

Serum lysozyme activity in patients with Hodgkin's disease undergoing chemotherapy.

Lysozyme activity was determined in undiluted and diluted sera of 41 patients suffered from Hodgkin's disease and of 40 healthy individuals. Blood was collected before chemotherapy, midway of its course, just after completion of chemotherapy and three weeks and three months later. Lysozyme activity was evidently increased as well in undiluted as in diluted sera in all our tested patients. These findings may be interpreted by the presence of immune complexes in sera which may stimulate the release of lysozyme from viable neutrophils. High activity in diluted sera probably is related to the increased concentration of lysozyme inhibitors. The applied chemotherapy did not significantly change the activity of the lysozyme during its course of even after its completion as found by three-month observation. The enhanced lysozyme activity in sera of patients may be at least in part to compensate decreased antibacterial and anticancer mechanisms.

Adolescent↗
PubMed1987

Nitroblue tetrazolium test in patients with Hodgkin's disease in the course of chemotherapy.

In 41 patients with Hodgkin's disease nitroblue tetrazolium (NBT) dye reduction with resting and stimulated granulocytes, before, in the course and after chemotherapy was examined. Twofold decrease of the capability of NBT reduction test with stimulated granulocytes was found, whereas with resting granulocytes only slight diminution was noted. Chemotherapy did not improve the tested parameters. No relationship was observed between the stage of disease and the presence of systemic symptoms and the NBT dye reduction. It can be assumed that granulocytes from patients with Hodgkin's disease are resistant to stimulation. The observations suggest that NBT test may be useful for evaluation of the function of granulocytes and to some extent for monitoring of the chemotherapy.

Adolescent↗
PubMed1986

System for testing the phagocytic capacity of human blood platelets.

We have elaborated a system for testing the phagocytic activity of human blood platelets. Blood was sampled to heparin and ACD and centrifuged. This procedure yields platelet rich plasma (PRP). As a substrate for phagocytosis we used Staphylococcus aureus 209P. The ratio of platelets to bacteria is 1:1. Incubation of bacteria and platelets continued up to 10 minutes. Lysostaphine was introduced to the phagocytic system to destroy all bacteria outside the blood platelets. The maximal values for the percentage of phagocytizing blood platelets were 0.7% and the phagocytosis index of 1.0 was reached at 6 minutes with incubation of bacteria and platelets. The authors pay attention to the role of blood platelets in antibacterial mechanisms.

Blood Platelets↗
PubMed1986

Does the blood platelet phagocytic capacity depend on the sex and plasma thrombopoietic activity?

In 16 men and 25 women we tested the phagocytic activity of blood platelets. In women a higher phagocytosis index, increased blood platelet count and lower percentage of phagocytizing cells could be noted in comparison to men. Blood platelet count was significantly higher in women than in men. From this group we chose 10 men and 10 women and determined the plasma thrombopoietic activity. The results obtained were divided into two subgroups A with high and B low plasma thrombopoietic activity. In subgroup A a higher blood platelet count and phagocytosis index and a decreased percentage of phagocytizing platelets in relation to subgroup B could be found. The phagocytic activity of blood platelets in relation to sex and plasma thrombopoietic activity is briefly discussed. The role of blood platelets in antibacterial mechanisms is emphasized.

Adult↗
PubMed1986

Phagocytic activity of human blood platelets examined by electron microscopy.

On the basis of a previously elaborated system for testing the phagocytic ability of the human blood platelets we have undertaken studies of testing the interaction between platelet and bacteria during phagocytosis examined by the electron microscope. We have found that during phagocytosis platelets lose their discoid shape and demonstrate numerous pseudopods. During 2 min a complete engulfment of the bacterium could be observed. 10 min after starting phagocytosis it is possible to note partially destroyed bacteria. The role of human blood platelets in the antibacterial mechanisms is briefly discussed.

Blood Platelets↗
PubMed1985

Cytochemical indices of leukocytes in patients with diabetes mellitus.

Cytochemical indices of leukocytes were determined in 16 patients with diabetes mellitus in the period of unbalancing and balancing. The following tests were made: content of glycogen and lipids, acid phosphatase (AP), alkaline phosphatase (AIP), myeloperoxidase (MPO) and nonspecific alpha-naphtol acetate esterase (NANAE) activity. In unbalanced diabetics an evident decrease in the activity of AP and MPO could be noted as well as a decrease of glycogen content and an increase of lipid content. An insignificant decrease could be observed in the activity of ALP and NANAE in granulocytes. A slight increase in the activity of NANAE in monocytes would be found. Balancing this disease induced the increase of all parameters in granulocytes except MPO activity. It is interesting to note that balancing diabetes mellitus deepened the observed changes in the decrease or increase of tested parameters. The presented findings clearly indicate the role of metabolic disorders in diabetes mellitus on the activity of some neutrophilic enzymes and the glycogen and the content of lipids in neutrophils.

Acid Phosphatase↗
PubMed1985

Serum lysozyme activity in patients with lung carcinoma subjected to radiotherapy.

Lysozyme activity was determined in undiluted and diluted sera of 30 patients with lung carcinoma and of 40 healthy individuals. Blood was collected before radiotherapy, in half of its course, just after completion of radiotherapy, and three weeks and three months later. In the controls the same time spacing was kept. Lysozyme was found to be increased both in undiluted and diluted sera. Enhancement of the lysozyme activity may be due to the presence of immune complexes (antigen--antibody) which are probably responsible for the release of the enzyme from the viable neutrophils. High activity in diluted sera indicates an increase of lysozyme inhibitors in sera of cancer patients. Radiotherapy has not induced significant changes in the enzyme, either during its course of after its completion as confirmed by three-month observation. Taking into consideration the role of the lysozyme in anticancer mechanisms it appears that determination of its activity may be of value in the course of cancer disease.

Adult↗