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Biomedical subjects

H Kamada

Publications and source records attributed to H Kamada.

At least 163 records · Page 9Linked to original sources
PubMed1993

[Alcohol consumption and brain adenylate cyclase system in rats: brain adenylate cyclase activity in alcohol-preferring AA and alcohol-avoiding ANA rats].

Brain adenylate cyclase (AC) activity in the alcohol-preferring AA (Alko Alcohol) and alcohol-avoiding ANA (Alko, Non-Alcohol) rats was examined. Both basal and Gpp(NH) p-stimulated AC activities were higher in the cerebral cortex of the ANA rats than in the AA rats. Isoproterenol-stimulated AC activity tended to increase in the cerebral cortex of the ANA rats compared to the AA rats. Basal AC activity in the striatum of the AA rats was higher than that in the ANA rats, however, striatal Gpp(NH)p-stimulated AC activity was not different between the two rats lines. In the mesolimbic and cerebellum, there was no difference in AC activity between the rat lines. These results suggest that the noradrenergic function in the cerebral cortex has an important role in the regulation of alcohol intake.

Adenylyl Cyclases↗
PubMed1992

Copper-metallothionein induction in the liver of LEC rats.

Recently, copper (Cu) was found to be unusually accumulated, suggesting the induction of metallothionein (MT) in the liver of LEC rats (Long-Evans rats with a cinnamon-like coat color), which develop spontaneous jaundice with hereditary hepatitis. Thus, the direct relationship between the unusual Cu accumulation and the induction of Cu-MT was investigated by giving LEC rats Cu-overloaded or Cu-deficient diets. Results based on the determinations of Cu and MT levels in several organs, as well as the gel-filtration profiles of the cytosols of liver homogenates, showed that dietary Cu induced Cu-MT and development of hepatic injury associated with jaundice.

Animals↗
PubMed1992

Isolation and characterization of a cDNA that encodes ECP31, an embryogenic-cell protein from carrot.

A full-length cDNA for ECP31, an embryogenic cell protein from carrot (Daucus carota L.) with a M(r) of 31,000 (Kiyosue T, Satoh S, Kamada H, Harada H (1991) Plant Physiol 95: 1077-1083), was isolated from a cDNA library prepared from embryogenic cells using PCR-amplified DNA as a probe. The genomic Southern blot analysis revealed that there are two or three genes for ECP31 in the carrot genome. The transcripts of ECP31 accumulated in the peripheral regions of clusters of embryogenic cells and disappeared in the course of somatic embryogenesis that was induced by transfer of the embryogenic cells to auxin-free media. The cDNA encodes a polypeptide of 256 amino acids, and the calculated molecular weight of this polypeptide is 26,111. The deduced amino acid sequence shows a high degree (62.2%) of similarity to that of a protein that is abundant during late embryogenesis of cotton (LEA D34; Baker JC, Steele C, Dure III (1988) Plant Mol Biol 11: 227-291). The mRNAs for ECP31 started to accumulate in zygotic embryos at a late stage of embryogenesis but were undetectable in mature embryos within 24 h after imbibition of seeds. In dry fruits (seeds), the transcripts were detected only in zygotic embryos by in situ hybridization. The level of ECP31 transcripts increased after treatment with abscisic acid (ABA) in torpedo-shaped somatic embryos but not in seven-day-old seedlings. These results suggest that both embryo-specific factor(s) and ABA are involved in the expression of the gene for ECP31.

Abscisic Acid↗
PubMed1992

Light requirement for shoot regeneration in horseradish hairy roots.

Hairy roots of horseradish (Armoracia rusticana) were induced by inoculation with Agrobacterium rhizogenes harboring Ri plasmid and cultured on phytohormone-free Murashige and Skoog medium after eliminating the bacteria. Hairy roots grew vigorously and sometimes formed yellowish calli under dark conditions. On the other hand, growth of hairy roots stopped after several weeks of culture with light, then shoots were regenerated. Frequency of shoot formation from hairy roots increased as the culture period in light lengthened and the light intensity increased. The shoot regeneration was induced by treatment with white or red light, but not with far-red light. Shoot regeneration by red light was inhibited by following treatment with far-red light. Red and far-red light reversibly affected shoot regeneration. Excised roots of nontransformed plants grew quite slowly on phytohormone-free Murashige and Skoog medium and occasionally formed shoots under white light conditions.

Journal Article↗
PubMed1991

Purification and immunohistochemical detection of an embryogenic cell protein in carrot.

An embryogenic cell protein from carrot (Daucus carota L.), designated ECP31 for embryogenic cell protein and with a relative mass of 31,000, was purified by sequential column chromatographies. Its apparent relative mass was estimated to be 120,000 by gel filtration. Immunoblotting and immunohistochemical studies showed that ECP31 was preferentially localized in the peripheral cells of clusters of embryogenic cells in the presence of 2,4-dichlorophenoxyacetic acid (2,4-D) and disappeared during the course of somatic embryogenesis in the absence of 2,4-D. ECP31 began to accumulate on the 33rd day after initiation of cultures of hypocotyl segments on Murashige-Skoog medium with 2,4-D, when callus began to appear on the segments. In dry seeds, lower amounts of ECP31 were located throughout the entire zygotic embryos but not in endosperm. ECP31 was also detected in provascular tissue of malformed somatic embryos.

Journal Article↗
PubMed1988

[Immunohistochemical study of developing rat embryo--localization of vimentin, GFAP, neurofilament protein within rat embryo central nervous system].

The identification of intermediate filaments within immature cells is valuable as a means to investigate neuronal and glial differentiation. The purpose of this study is to investigate the time of appearance of neuron and glia within rat embryo by identifying cell-specific intermediate filaments. Pregnant rat were daily sacrificed from day 9-20 of pregnancy. Rat embryos were fixed with 100% ethanol and then we have made the paraffin section. We have stained the specimens for vimentin in accordance with ABC method and for GFAP in accordance with PAP method. We have performed Bodian stain. The neural plate of day 9 rat embryo is composed with vimentin positive immature cells which are disposed with pseudostratified arrangement. In midbrain of day 13 rat embryo, immature cells and their fibers are vimentin positive which are spanning from ventricular surface to marginal layer. NFP is detectable in immature cells and fibers of marginal layer. But GFAP positive cells and fibers are not identified in the same region. In anterior column of day 18 rat embryo, GFAP positive fibers are detectable. Summarizing of these results, we can display first: immature cells composing of neural plate are vimentin positive at day 9, second: NFP positive fibers and cells appear at day 13, third: NFP positive cells are vimentin positive, too, forth: GFAP positive fibers appear at day 18 and they are vimentin positive, too.

Animals↗
PubMed1987

[Cerebral amyloid angiopathy complicated by multiple cerebral infarcts and intracerebral hemorrhages: case report].

Recently, cerebral amyloid angiopathy is stressed as an unusual and infrequent cause of cerebral infarct or intracerebral hemorrhage. This report described a case of cerebral amyloid angiopathy complicated by multiple cerebral infarcts and multiple intracerebral hemorrhages. This 70-year-old man was admitted to our hospital on November 10, 1984 for evaluation of the gradual onset of dysarthria. Examination showed only slight dysarthria. There was no history of hypertension or dementia. A computed tomography (CT) showed enlarged ventricles with cortical atrophy and multiple low-density lesions, deep in the left frontal, left parietal, right parietal lobes, and in the both basal ganglias. The patient discharged from the hospital with only slight dysarthria. On November 20, 1984, he was admitted to our hospital again, because he was found unresponsive on the floor. He was somnolent but arousable. Examination showed disorientation, impairment of recent memory and impairment of calculation, A CT scan demonstrated three small intracerebral hemorrhages in the left frontal, right parietal lobes and left basal ganglia. On the fifth hospital day he deteriorated acutely, becoming semicomatose and hemiparetic on the right side. A repeated CT scan showed two new intracerebral hemorrhages in the left frontal lobe. Cerebral angiograms showed only minimal changes due to the occupying lesions in the above mentioned area. The hematomas was evacuated via left fronto-parietal craniotomy. The specimens removed with hematoma (stained with hematoxylin-eosin, Congo red and thioflavin T) showed extensive amyloid angiopathy. Postoperatively he made a good recovery, although he had residual mental dysfunctions. He expired by pneumonia on March 30, 1985.(ABSTRACT TRUNCATED AT 250 WORDS)

Aged↗
PubMed1986

Auxin-controlled glycoprotein release into the medium of embryogenic carrot cells.

Glycoproteins released from carrot cells into culture media were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and visualized by staining with Coomassie brilliant blue or with the periodic-acid Schiff procedure. The appearance or disappearance of two glycoproteins of M(r) 65,000 (GP65) and M(r) 57,000 (GP57) was closely related to the formation of somatic embroys. GP65 was released specifically from embryogenic cells cultured in a medium without 2,4-dichlorophenoxyacetic acid, in which they can form somatic embryos. GP57 was released from the same embryogenic cells, if they were cultured in a medium with 2,4-dichlorophenoxyacetic acid, in which they cannot form somatic embryos. Nonembryogenic cells which cannot form somatic embryos, released only GP57.

Journal Article↗
PubMed1986

[A case of spontaneous carotid-cavernous fistula disappearing following irradiation].

A case of spontaneous carotid-cavernous fistula, which completely disappeared following irradiation (45 Gy), is reported. A 59-year-old woman was admitted on October 10, 1983, with a history of doplopia and recurrent retrobulbar and occipital pain. On admission, neurological examination showed no neurological deficit, and right cerebral angiography demonstrated a dural internal carotid-cavernous fistula fed by the meningohypophyseal trunk and drained into the inferior petrosal sinus. In spite of the conservative management, right oculomotor palsy and hypesthesia corresponding to the division of ophthalmic branch of the right trigeminal nerve was developed. On November 22, liniac irradiation to the region of fistula (30 days, total dose, 45 Gy) was started. Symptoms and signs gradually improved 3 weeks after irradiation and completely disappeared 3 months after irradiation. Carotid-cavernous fistula was no longer demonstrated by the cerebral angiography 7 months after irradiation. Two years after onset, no recurrence of symptoms was observed.

Arteriovenous Fistula↗
PubMed1985

[Co-existence of renal cyst and tumor: report of two cases and a review of the literature].

We report on two patients with renal cyst and tumor in the same kidney. Despite the diagnosis of benign renal cyst obtained by radiological investigation, one case proved to be malignant at surgical exploration. The other patient was diagnosed to have renal cyst and tumor in the same kidney preoperatively. Co-existence of renal cyst and tumor is considerably rare. Thirty four cases collected from the Japanese literature, including our two cases, are reviewed and discussed.

Adenocarcinoma, Papillary↗
PubMed1985

[Pituitary apoplexy with an unruptured carotid-ophthalmic aneurysm].

The association of pituitary adenoma and adjacent cerebral aneurysm is not uncommon and acute hemorrhage into a pituitary adenoma is also a well recognized condition. However, the simultaneous occurrence of pituitary apoplexy with intracranial aneurysm is very rare. Such a case demonstrates the diagnostic difficulty in distinguishing between pituitary apoplexy and rupture of an aneurysm. We reported a patient with subarachnoid hemorrhage in whom a hemorrhage into the pituitary adenoma and a carotid-ophthalmic aneurysm was proven, and discussed the differential diagnosis and treatment. A 41-year-old man, who developed sudden severe headache with nausea and vomiting, was admitted to our hospital. Examination disclosed a mildly stuporous man with bilateral defects of upper lateral visual fields and lumbar puncture revealed subarachnoid hemorrhage. Plain radiographs of the skull showed an enlarged and eroded sella turcica. Carotid angiography revealed a left carotid-ophthalmic aneurysm. A plain CT scan demonstrated an acute suprasellar hematoma. A transsphenoidal operation was performed and postoperative course was uneventful.

Adult↗
PubMed1984

[The immunohistological study of developing human spinal cord--the localization of vimentine, GFAP in radial glial cell].

The morphological changes of radial glial cells in developing human spinal cord have been studied immunohistologically. The specimens extracted from developing human spinal cords, gestational age of 15 weeks, 30 weeks and 35 weeks, were prepared with cryostat for the investigation of anti vimentin mouse monoclonal antibody and of GFAP rabbit serum. We have stained the specimens for vimentin in accordance with ABC method, for GFAP in accordance with PAP method. On gestational age of 15 weeks, the fibers radially oriented from the central canal to pial surface were positive for vimentin and GFAP. Partially, early differentiated astrocytic cells were found. On gestational age of 30 weeks, radially oriented fibers were positive for vimentin and GFAP. They took more tortuous course, branching than those of 15 weeks. More differentiated astrocytic cells were found. On gestational age of 35 weeks, the staining for vimentin was very weak on radially oriented fibers and these fibers were more tortuous, branching than those of 30 weeks. More differentiated astrocytic cells than those of 30 weeks were found in rich amount. From results above, we concluded: 1) Radial glial cells in developing human spinal cord were positive for vimentin and GFAP. 2) The show weaker staining for vimentin in process of gestational age. 3) They differentiate to astrocyte. 4) They appear in earlier stage than suggested by previous reports.

Astrocytes↗