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Biomedical subjects

H J Glander

Publications and source records attributed to H J Glander.

At least 73 records · Page 4Linked to original sources
PubMed1982

[Net K+(86Rb+) uptake of human lymphocytes following phytohemagglutinin stimulation].

We tried to develop a short-time incubation method for the early detection of lymphocyte stimulation due to lectins and antigens. For this purpose the netto-K+(86Rb+)- uptake of (1) nonstimulated, (2) phytohaemagglutinin (PHA) activated and (3) potassium dichromate (K2Cr2O7) activated human lymphocytes was examined by means of 86Rb+ as a marker. The relative netto-K+-uptake was reduced by 38 per cent (p less than or equal to 0.01) on average after the incubation with PHA. After the incubation with K2Cr2O7 the 86Rb+-values differed, however, only unessentially from the nonstimulated controls in one patient sensibilized by K2Cr2O7. We concluded from our results that this method is not suitable for the early detection of cell mediated immune reactions.

Humans↗
PubMed1982

The influence of the cryopreserving process on rubidium uptake by human spermatozoa.

The effect of cryopreservation and its phases on the netto-Rb+ uptake (analogous to the K+ uptake) of washed human spermatozoa was investigated under different conditions. The Rb+ uptake reached a steady state after almost 50-min incubation both before and after the cryopreserving process. The Rb+ uptake could be reduced to about 30% by ouabain and to about 80% by monoiodoacetate. The cryoprotective agents glycerol (G), ethylene glycol (EG), and particularly dimethylsulfoxide (DMSO) reduced the Rb+ uptake by a factor dependent on its concentration. The comparative cryopreservation with G, EG, DMSO,and dimethylformamide (DMFA) as cryoprotective agents caused an inhibition especially of the ouabain-sensitive netto-Rb+ uptake. This inhibitory effect became stronger from G to EG and from DMSO to DMFA. Within the course of cryopreservation especially the process of pelletation, Rb+ uptake was reduced, whereas the prolongation of the equilibration time up to 180 min moderated the effect of the cryopreservation on the Rb+ uptake. Beside the reduction of the absolute Rb+ uptake, the cryopreservation altered the dependence of the netto-Rb+ uptake on the extracellular Rb+ concentration in a sigmoid manner.

Dimethyl Sulfoxide↗
PubMed1982

[Current determination of ovulation time. 2. Optimization of timing for oocyte recovery].

Knowledge of probable ovulation date is essential to the recovery of pre-ovulatory oocytes for in vitro fertilisation. The authors used a commercially available LH-RIA kit for blood and urine samples together with the HI-Gonavis test for urine assay for high-accuracy determination of luteinising hormone (LH) in the peri-ovulatory cycle phase. Follicle status and oocyte quality were laparoscopically described of seven patients, between the eleventh and 16th days p.m. Optimum timing for oocyte recovery has not even become known by knowledge of LH rise. Different recommendations by other authors are discussed, and reference is made to unanswered problems.

Body Temperature↗
PubMed1981

[Comparison between cryoprotective potentials of glycerol, dimethylsulphoxide, dimethylformamide, and ethylene glycol in cryopreservation of human semen (author's transl)].

The cryoprotective effects of dimethylsulphoxide, dimethylformamide, and ethylene glycol in definite concentrations of 3.5, 7.0, and 12.0 per cent were compared with glycol action in cryoprotective preservation of human semen. Motility behaviour in response to laboratory stress (due to incubation at + 40 degrees C) as well as respiratory activation quotients for succinate, digitonin, and 2.4-dinitrophenol were used as criteria of assessment. The cryoprotective effect of glycerol was in no single instance surpassed by any of the other compounds, with the concentrations tested.

Cryoprotective Agents↗
PubMed1980

[Psychological aspects relating to artificial donor insemination (ADI) (author's transl)].

Discussed in this paper is the psychic situation of sterile couples who resort to artificial insemination, using donor semen (ADI). --Careful psychodiagnostic checking prior to ADI seems to be essential to forestalling psychic problems. ADI treatment should be applied solely to really sterile couples of stable psychic status and with good communication between the two partners. A method of psychological preparation for ADI is described. Psychodiagnostic examinations should be followed by specific gynaecological checks. Such approach will yield at least two advantages: 1. Gynaecological examination will not be necessary, if contra-indications are concluded from psychological checking 2. Early psychological assessment of sterile couples will become possible. Favourable psychic development of both couples and children born as a result of ADI will be an additional advantage of high probability.

Attitude↗