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Biomedical subjects

H Inomata

Publications and source records attributed to H Inomata.

At least 127 records · Page 7Linked to original sources
PubMed1991

Corneal choristoma with microphthalmos.

We report two rare cases of corneal choristoma with microphthalmos. In both cases, the whole thickness of the cornea, as well as the anterior chamber and iris, was replaced by cutaneous and subcutaneous tissue. In case 1, diagnosed as dermoid choristoma, the cutaneous tissue contained skin appendages and subcutaneous fibrofatty tissue extended into the vitreous cavity. The fibrous mass in the cavity contained fragments of retina. In case 2, diagnosed as a dermislike choristoma, the cutaneous tissue lacked skin appendages. These choristomatous cutaneous tissues hindered normal growth of ocular structures, resulting in microphthalmos. Use of computed tomography was helpful in evaluating the extent of ocular tissue involvement and accompanying intraocular malformations.

Choristoma↗
PubMed1991

Class II major histocompatibility complex on melanocytes of Vogt-Koyanagi-Harada disease.

The eyes obtained from two Japanese autopsy cases of patients with Vogt-Koyanagi-Harada disease were immunohistochemically examined. Both patients, a 63-year-old woman and a 68-year-old man, were clinically and histologically diagnosed as having Vogt-Koyanagi-Harada disease. Immunohistochemically, the choroidal infiltrate was composed predominantly of T lymphocytes with a larger proportion of helper/inducer T cells than suppressor/cytotoxic T cells and it also included activated lymphocytes expressing CD26 and CD25 antigens. Class II major histocompatibility complex was expressed in the choroidal melanocytes as well as in the endothelium of the choriocapillaris. Depositions of complement, however, were focally noticed in the choroid. Our results indicate that the cell-mediated immune process plays an important role in the development and progression of Vogt-Koyanagi-Harada disease, while choroidal melanocytes appear to play a pathogenic role in this disease.

Aged↗
PubMed1991

Immunohistological and electron microscopical study of nodular fasciitis of the orbit.

We report the case of a 7-year-old Japanese girl with nodular fasciitis which was investigated by immunohistological and electron microscopical methods. An excised nodular lesion in her right orbit showed characteristic histological features of the disease. The fibroblastic cells showed myofibroblastic characteristics, such as immunohistochemically positive reactions against muscle specific actin and vimentin and characteristic electron microscopical appearances. The multinuclear giant cells did not react against any histiocytic markers, including HLA-DR, antimacrophage antigen, lysozyme, and S-100 protein, but the myofibroblastic markers and the electron microscopical study did reveal myofibroblastic characters.

Child↗
PubMed1991

Altered permeability of iris vessels following posterior chamber lens implantation: an electron microscopic study.

Altered permeability of the iris vessels of monkey eyes following extra-capsular cataract extraction and posterior chamber intraocular lens implantation was studied by transmission electron microscopy using horseradish peroxidase (HRP) as a tracer. In control animals, HRP-reaction products were confined to the vascular lumens and to a small number of vesicles on the luminal side of the endothelial cells. In experimental animals, however, the interendothelial junctions of the iris vessels were filled with HRP-reaction products, and the endothelial cells of the iris vessels contained a large number of HRP-labeled plasmalemmal vesicles that opened into the basal lamina. HRP-reaction products were also found in the surrounding iris stroma of these experimental animals.

Animals↗
PubMed1991

[A role of cultured bovine lens epithelial cells in the fibrinolytic system].

The release of plasminogen activators (PAs) and plasminogen activator inhibitor (PAI) from cultured bovine lens epithelial cells was investigated, in an attempt to study the effect of lens epithelial cells on the fibrin deposit and fibrinolytic process on the surface of IOL. Cultured bovine lens epithelial cells released tissue plasminogen activator (t-PA) and type 1 plasminogen activator inhibitor (PAI-1). t-PA activity was completely inhibited by PAI-1. These findings suggest that lens epithelial cells cause suppression of fibrinolysis in the anterior chamber of the eye.

Animals↗
PubMed1990

Cellular adhesiveness on implanted lenses in monkeys.

Cells are known to adhere to implanted intraocular lenses (IOLs), but the mechanisms of this adhesiveness are not known. We studied cellular adhesiveness on four posterior chamber IOLs that had been implanted into monkey eyes. The animals were killed at 4 and at 7 days after lens implantation. The IOLs were removed and examined by transmission electron microscopy. At 4 days after IOL implantation, macrophages were attached to the IOL surface; at 7 days after implantation, multinucleated giant cells were attached to the IOL surface. These cells had bundles of microfilaments in the subplasmalemmal region of areas of close cell-IOL apposition. These microfilaments may play an important role in the cellular adhesiveness on the surface of implanted IOLs.

Animals↗
PubMed1990

Histological study of choroidal melanocytes in animals with tapetum lucidum cellulosum.

The distribution and morphology of choroidal melanocytes in dogs and cats which have a tapetum were compared with those of humans who do not. In dogs or cats, tapetal cell-like melanocytes were arranged in layers on the scleral side of the tapetum and underneath the choriocapillaris in the non-tapetal area. Although the tapetum of the dog occupied a smaller area than that of the cat, the tapetum plus the area of tapetal cell-like multilayered melanocytes occupied most of the fundus in the dog in almost the same way as in the cat. These multilayered melanocytes contained few intracytoplasmic organelles except for melanin granules, and some had regularly arranged melanin granules. In human eyes tapetal cell-like melanocytes were not found anywhere. It was concluded that the morphology and structural architecture of choroidal melanocytes of dogs or cats are different from those of human eyes and closely correspond to the tapetum.

Adult↗
PubMed1990

Cellular reaction on the surface of intraocular lenses removed from human eyes.

We studied four angle-supported anterior chamber lenses whose optics and haptics were made of poly(methyl methacrylate). All four lenses had been removed from human eyes because of bullous keratopathy. Using light and electron microscopy, we compared the cellular reaction on the optics, which had been in the anterior chamber, with that on the haptics, which had been in touch with the iris stroma. A typical foreign body granuloma was seen around the haptics, while the optics were free of foreign body reaction.

Aged↗
PubMed1990

Uveitis induced by various cross-reactive antigens in guinea pigs.

In order to investigate possible immunopathogenic mechanisms in the recurrence of uveitis, cross-reactive proteins were tested for their capacity to induce experimental uveitis. Guinea pigs were immunized with porcine serum albumin (PSA) in complete Freund's adjuvant (CFA) by subcutaneous injection. Fourteen or 28 days after the immunization, PSA, bovine (BSA), sheep (SSA), equine (ESA), rabbit (RSA) serum albumin, bovine gamma globulin (BCG) or ovalbumin (OA) was injected into the vitreous. Uveitis occurred in the eyes injected with PSA, BSA, SSA, ESA or RSA, but not BGG or OA. Serum antibodies and erythematous delayed-type skin reactions against PSA, BSA, SSA, ESA and RSA were positive in animals immunized with PSA in CFA. In an adoptive transfer study, humoral and cellular immunity recognized cross-reactive antigens and uveitis developed. Once a guinea pig is sensitized, uveitis may occur or recur from subsequent intravitreal challenge by antigens that are not completely the same but have a cross-reactivity with the immunizing antigen.

Animals↗
PubMed1990

Recurrent herpetic keratitis: failure to detect herpes simplex virus infection using the Syva MicroTrak HSV1/HSV2 direct specimen identification/typing test.

A 35-year-old man had developed recurrent herpetic keratitis characterized by dendritic keratitis at intervals of a year. We were able to culture cytopathic agents repeatedly from his lesions by inoculating Vero cells. The cultures yielded definitive evidence of a virus that caused a cytopathic effect within 3 days. However, these virus strains could not be identified as herpes simplex virus (HSV) in immunofluorescence assays using the Syva MicroTrak HSV1/HSV2 direct specimen identification/typing test. Rather they were identified as strains of HSV type 1 (HSV-1) on the basis of plaque morphology, neutralization tests, electron-microscopic examination and DNA restriction endonuclease analysis. Our results allow us to assume the existence of HSV-1 strains isolated clinically that are negative to analysis using the Syva Micro-Trak HSV1/HSV2 direct specimen identification/typing test.

Adult↗
PubMed1990

Immunohistochemical studies of Vogt-Koyanagi-Harada disease with sunset sky fundus.

We studied Vogt-Koyanagi-Harada disease (VKH) with sunset sky fundus using histopathology and immunohistochemical methods. The materials examined were 4 eyes obtained at autopsy on two patients with VKH. The first patient died 32 months after the onset of VKH, and the other 7 years after onset. Histopathology of the eyes showed scattering infiltration of lymphocytes in the thickened choroid with a remarkable disappearance of choroidal melanocytes. T and B lymphocytes were identified by immunohistochemistry, using monoclonal antibodies. Approximately 70% of the lymphocytes were T cells. In the case 1 patient, the ratio of CD4+ to CD8+ T cells was 2 to 3. Thus, the evidence of T and B lymphocytes in these uveal tissues indicates that the inflammation remained active even at the convalescent stage.

Aged↗
PubMed1990

[A case of Rubinstein-Taybi syndrome suspected with goniodysgenetic glaucoma].

We reported a case of Rubinstein-Taybi syndrome suspected in association with goniodysgenetic glaucoma, and studied using light and electron microscopy the anterior chamber angle tissues obtained surgically by trabeculectomy. The patient was 31-year old male, who had a systemic appearance of Rubinstein-Taybi syndrome with dwarfism, mental retardation, antimongoloid slant, flat-broad based thumbs, low set ears, high arched plate except for whorl of dermatographism. In addition to these malformations, goniodysgenetic glaucoma was also present which is characterized by underdevelopment of the angle recess and invisible ciliary body band in gonioscopic examination. The histopathological studies of the specimens revealed the presence of a compact tissue filled with a large amount of collagen fibers with few cells in the juxta-canalicular tissue of Schlemm's canal. There were 3 to 4 layers of trabecular sheets of corneoscleral meshwork at the anterior chamber side of the compact tissue. We conclude that the presence of the compact tissue under Schlemm's canal represents goniodysgenesis, underdevelopment of the trabecular meshwork, which is the primary cause of the glaucoma in this case.

Adult↗
PubMed1990

[Interferon-alpha for the treatment of retinal vasculitis associated with human T-lymphotropic virus type I myelopathy (HAM)].

Interferon-alpha was effective for the treatment of retinal vasculitis and vitreous opacity in a patient with human T-lymphotropic virus type I associated myelopathy (HAM). The patient was a 40-year-old male with retinal vasculitis, vitreous opacity and Koeppe's iris nodules. Systemic administration of corticosteroid hormone was not fully effective for the treatment of ocular involvements except for iris nodules. After Interferon-alpha treatment, given 3 million IU/day as the intramuscular injection for 4 weeks, the retinal vasculitis subsided and vitreous opacity disappeared.

Adult↗
PubMed1990

[Identification of cells in deposits on the intraocular lens surface].

We performed posterior chamber lens implantation in a monkey eye and removed the lens on the 14th day after implantation. Cellular deposits on the IOL were observed by a dissecting microscope. Two kinds of cells, large and small, were observed on the lens surface. The large cells constituted the major proportion of the deposits. The same cellular deposits, which were observed by the dissecting microscope, were examined by scanning electron microscopy and then by transmission electron microscopy. The large cells had the structural features of multinucleated giant cells, and the small cells were macrophages.

Animals↗
PubMed1990

[Iris nevus (Cogan-Reese) syndrome--clinicopathological correlations].

Clinicopathological correlations of iris nevus (Cogan-Reese) syndrome were studied by light and electron microscopy, using tissues obtained surgically by trabeculectomy and peripheral iridectomy. The patient was a 52-year-old female, who had a typical appearance of the disease with nodular iris nevi, distorted pupil, ectropion uveae, peripheral anterior synechia, and intractable glaucoma. The histopathological studies of the specimen revealed abnormal corneal endothelialization and basal lamina formation continuously covering the peripheral cornea, trabecular meshwork, and anterior iris surface. Lymphocyte infiltration was found in the layer of the endothelialization and in the anterior iris stroma. The nodular iris nevi were found to consist of mainly integration of degenerated iris melanocytes and clump cells of Koganei. Neovascularization associated with infiltration of lymphocytes and macrophages was also observed in the trabecular meshwork. The present study suggests that the disease might occur by chronic inflammation primarily as corneal endothelitis and iritis.

Endothelium, Corneal↗