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Biomedical subjects

H Ide

Publications and source records attributed to H Ide.

At least 379 records · Page 21Linked to original sources
PubMed1985

Effects of cyclic AMP on limb bud chondrogenesis in low cell density culture.

In a previous paper, it was shown that the limb bud mesodermal cells differentiated into cartilage even at low cell density by lowering the serum content in the culture medium (Hattori & Ide, Exp cell res 150 (1984) 338) [20]. The present paper describes the effects of cAMP on limb bud chondrogenesis at low cell density. cAMP promoted chondrogenesis at low cell density in cultures with various concentrations of serum. The limb bud cells differentiated into cartilage cells without forming aggregates. cAMP inhibited the loss of chondrogenic capability in serum-rich medium. The relationship between cAMP level and serum content is also discussed.

Animals↗
PubMed1985

Fish melanin-concentrating hormone disperses melanin in amphibian melanophores.

Melanin-concentrating hormone (MCH), which was isolated from salmon pituitary and caused melanin concentration in fish scale melanophores, has been tested on cultured chromatophores of an amphibian, the bullfrog tadpole. MCH induced dispersion of melanin in cultured melanophores of the tadpole. The duration of the dispersing effect of MCH was relatively short compared with that of alpha-melanocyte-stimulating hormone (alpha-MSH). MCH also induced the concentration of cultured iridophores of bullfrog tadpole.

Animals↗
PubMed1985

Characteristics of pulmonary function in patients with diabetes mellitus.

The incidence of pulmonary diseases among diabetics in Japan has been found to be more than 50% at death. This suggests the existence of some pulmonary risk factors. We analyzed pulmonary functions in 50 diabetics (31 males and 19 females) without overt lung disease, compared to 21 healthy male subjects of the same age (around 50 years old). Forced vital capacity and timed vital capacity were lower in diabetics (P less than 0.005). Diffusing capacity was also decreased in male diabetics (P less than 0.05). Among diabetics, a decrease in the diffusing capacity was dominant in patients with diabetic retinopathy, which correlated with an increasing duration of their diabetes. Analysis by the partial correlation method which accounts for the smoking index showed that standardized indices for peripheral airflow in male diabetics decreased significantly as the patient's age increased (P less than 0.005); the rate of decrement was greater than that of the control subjects. Diabetic patients showed abnormal lung function in the peripheral airways which increased with age and gas transfer was also affected by diabetic microangiopathy as well as the duration of diabetes. These changes seemed to deteriorate progressively, possibly combining and contributing to respiratory insufficiency in critical pathological conditions.

Adult↗
PubMed1985

Phosphatidate phosphatase in rat liver: the relationship between the activities with membrane-bound phosphatidate and aqueous dispersion of phosphatidate.

Gel filtration of rat liver cytosol on Bio-Gel A-5m resolved the phosphatase activities into four peaks, all of which showed activity with either phosphatidase bound to microsomal membrane (PAmb) or phosphatidate dispersed in sonicated microsomal lipid (PAaq) as the substrate. A major part of the PAmb phosphatase activity (52%) was eluted in a peak with an apparent molecular weight (Mr) of 500,000 where the PAaq phosphatase activity was very low. A major PAaq phosphatase activity peak (48%) was obtained in the void volume (Vo), where the PAaq phosphatase activity was higher than the PAmb phosphatase activity. The addition of 0.075% Tween 20 to the elution buffer gave only the 500 kilodalton (kDa) peak. When the activity in the Vo peak obtained in the absence of Tween 20 was rechromatographed in the presence of the detergent, a part of the activity was dissociated into 500 kDa molecules having a preference for PAmb. These results suggest that the enzymes obtained in the Vo peak are formed by the association of the 500 kDa molecules with macromolecules and that the substrate preference of phosphatidate phosphatase is modified by the change in the physical state of the enzyme. The microsomal phosphatidate phosphatase activities were also separated on Bio-Gel A-5m after solubilizing by sonication. Most of both the PAmb and PAaq phosphatase activities were coeluted in the Vo peak, in which the latter activity was higher than the former. When the gel filtration was performed in the presence of Tween 20, a major activity peak with a preference for PAmb was obtained at the elution volume corresponding to apparent Mr 500,000, indicating a potential relationship between the cytosolic and microsomal activities.

Animals↗
PubMed1985

[Multitherapy of advanced esophageal carcinoma with special reference to pre- and post-operative administration of cis-platinum].

We discussed the results of multitherapy after pre- and post-operative administration of cis-platinum (CDDP) in resected cases of advanced esophageal carcinoma. The patients were, pre-operatively, dosed in the following ways; 1) CDDP (50 mg, 1/week) X 2-3, 2) CDDP (50 mg, 1-week) +BLM (15 mg, 1/week) X 2-3 or +MTX (30mg, 1/week) X 2-3. The patients under went surgery 1 to 2 weeks later. Post-operative administration of CDDP + MTX, or CDDP + BLM was performed 3-4 times about 3 weeks after surgery. Intra-operative and/or post-operative irradiation was also performed. CDDP was administered pre-operatively in 11 cases who ranged in age 45 to 71. Regarding stage, 2 were stIII and 9 were stIV; and curabilities where cIII in 2 cases, c I in 5 and c 0 in 4. With regard to surgery, the right thoracic and abdominal approach was adopted in 7 cases and the left thoracic and abdominal approach adopted in 3. Blunt dissection was performed in 1 case. We have never experienced post-operative complication of histological effects noticed in resected specimens, Ef1 was evaluated in 3/11 and Ef2 was noticed in 2/11. Patients who achieved an Ef2-1 effect due to the administration of CDDP as part of the multitherapy made satisfactory progress. On the other hand, patients who did not react to CDDP and who had marked vascular invasion died after a short period.

Aged↗
PubMed1985

[Clinicopathological study of esophageal cancer in the early stage and its later development].

Ninety-five specimens taken from 82 cases of superficial esophageal cancer limited within the submucosal layer were studied to clarify the developmental changes of early esophageal cancer. Esophageal epithelium was also open to cancerous or dysplastic changes. Cancers limited to the mucosal layer were grossly classified as follows. 1) those progressing to carcinoma in situ of several millimeters to 2-5 cm in size, then invading deep beyond the mucosa, and 2) those invading beyond the mucosal layer to proliferate before reaching several millimeters in extent. Most of the resected cases belonged to type 1. which was flat in form with redness, erosive appearance or sclerohypertrophic appearance of the mucosa. These findings depended on the degree of cellular atypism and histological differentiation. Almost all submucosal cancer lesions less than 2 cm in size were of macroscopically protruding or depressed type. Many cases were simple type without intraepithelial spreading around the primary lesion and seemed to develop with in micro-cancer nests. Superficial circularly spreading cancer was one of the complex types observed with intraepithelial cancer around the primary lesion. Most superficial spreading cancers originating as large flat epithelial lesions, and the protrusions or depressions found in the lesions were considered to have developed as secondary changes occurring during the course of change. Vessel-invasion of superficial esophageal cancer was also discussed.

Carcinoma in Situ↗
PubMed1985

[A roentgenographical study on the aging process of alignment of the lower extremities in children--with special reference to pain in the lower extremity].

To date, detailed reports are not available in large number concerning the alignment of lower extremities of children. Moreover, those already published are primarily concerned only with normal children, failing to make a sound comparison of lower extremity alignment between normal children and children with complaints about lower extremity alignment. Following is the summary of extensive research the author has done by comparing statistical data obtained--such as measurements of condylar distance, malleolar distance, and alignments of lower extremities--from 269 cases of normal children (538 lower extremities), from 2 years to 15 years, and 173 cases of children with pain (215 lower extremities), from 3 years to 15 years. Most of the children aged five years and older, whose main complaint is pain in their lower extremities, were found to show significant variations in the alignment of their lower extremities and were compared to normal children. When the intercondylar distance is greater than 10 mm, it is imperative to carefully investigate the alignment of the patient's lower extremities, because the pain is almost always found due to the malalignment. Bow legs and knock knees are not only deformity but also frequently accompanied by pain. Therefore, Lanz's method is most useful to evaluate bow legs and knock knees.

Adolescent↗
PubMed1985

[A case of esophageal carcinoma with liver metastasis treated with cisplatin].

A patient with liver metastasis of esophageal carcinoma was treated with Cisplatin and showed complete response both in the esophageal region and liver metastasis. Combination chemotherapy using Cisplatin is now often employed against metastatic regions of epidermoid carcinoma of the esophagus, and shows certain degrees of response. Cisplatin is considered to be one of the most effective agents for the treatment of epidermoid carcinoma of the esophagus.

Carcinoma, Squamous Cell↗
PubMed1985

[A few important aspects in the treatment of early esophageal cancer].

The Japanese Society for Esophageal Cancer has defined mucosal and submucosal cancer as superficial cancer of the esophagus. Superficial cancer without metastasis was defined as early cancer of the esophagus. Of 104 cases of superficial cancer resected in these twenty years, early cancer was 62 cases, the incidence being 4.9% of all resected cases. The operative mortality rate and 5-year-survival rate of early cancer were 1.9% and 62%, respectively. As for the depth of the cancerous invasion, 14 of 104 superficial cancer cases were mucosal cancer. Twelve of 14 mucosal cancer belonged to early cancer. Vascular invasion was noted in one of 12 early mucosal cancer cases. No cancer death has been encountered in the early mucosal cancer cases. So the discovery of the mucosal cancer is mandatory to obtain the excellent results. For this purpose the value of endoscopy including the dying method, should be emphasized. In terms of treatment of the early cancer no special surgical procedure has been considered. The lymph node dissection has been carried out systematically. Some kinds of postoperative combined treatments are performed for early cancer cases with vascular invasion. The reduction surgery and the noninvasive treatment will be advisable for the small mucosal lesions.

Aged↗
PubMed1984

Cytophotometric DNA analysis of mucosal and submucosal carcinoma of the esophagus.

Cell nuclear deoxyribonucleic acid (DNA) content was microspectrophotometrically determined in 35 cases of mucosal and submucosal carcinoma of the esophagus. DNA distribution pattern was classified into types I, II, III, and IV, according to the degree of dispersion on the DNA histogram, in the order of wider distribution. Patients with types I and II (relatively regular in DNA distribution) had an uneventful postoperative course and no recurrence, whereas 3 of 15 (20%), and 5 of 9 (55.6%) with type III and type IV, respectively (widely scattered DNA distribution), died following a recurrence. Cytophotometric DNA analysis of the cancer cells reflected well the outcome in patients with esophageal carcinoma. These results suggest the potential usefulness of cytophotometric DNA analysis for assessing the prognosis, even in the early stage of esophageal carcinoma.

Adult↗