Human glioma cells derived from biopsied material were grown in tissue culture for periods of up to 2 weeks. Supernatants from these cultures were harvested at various intervals and their ability to enhance proliferation of cultured umbilical vein human endothelial cells was assessed. All of the supernatants tested contained some degree of endothelial cell mitogenic factor activity. The activity in the supernatants reached a maximum from cultures which were 4-8 days old and gradually decreased thereafter. Supernatants from the fibroblast monolayer were not significantly stimulatory for this endothelial cell model. On the other hand, supernatants from the glioma cultures did not increase the growth of human skin fibroblast or peripheral blood lymphocytes in culture. Extracts from bovine cartilage inhibited the mitogenic activity of the supernatants from glioma cultures.

Angiogenesis Inducing Agents↗

PubMedDate not supplied

The role of human endothelial cells in allograft rejection. Some in vitro correlates.

Endothelial cells, separated from the inside of the umbilical vein by collagenase digestion, could clearly stimulate allogeneic lymphocytes to blastogenesis and increased DNA synthesis in mixed lymphocyte endothelial-cell cultures. Many of the characteristics of these mixed cultures were similar to those found in mixed cultures consisting exclusively of allogeneic lymphoid cells; the MLC response. Endothelial cells could also be destroyed in vitro by cells sensitized in mixed lymphocyte cultures. When incubated in the presence of specific HL-A antisera, they were destroyed in non-immune peripheral blood mononuclear cells. These three reactions are thought to be in vitro correlated to some of the events taking place following allografting in vivo.

Antibodies↗