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Biomedical subjects

H Hirschberg

Publications and source records attributed to H Hirschberg.

At least 91 records · Page 5Linked to original sources
PubMed1978

The role of macrophages in the lymphoproliferative response to Mycobacterium leprae in vitro.

Peripheral blood lymphocytes from patients suffering from lepromatous leprosy do not normally react in vitro to stimulation by Mycobacterium leprae antigens. In contrast, we found that T cells from non-responding patients in combination with macrophages from responding patients or healthy contacts did respond well to M. leprae. Conversely, T cells from responding patients or healthy contacts in combinations with macrophages from non-responding patients failed to respond. It seems, therefore, that the lack of response normally observed in in vitro tests using cells from lepromatous leprosy patients is due to a failure of their macrophages to present M. leprae antigens in an immunogenic form.

Antigens, Bacterial↗
PubMed1977

Inhibition of human mixed lymphocyte culture stimulation by anti-HLA-D-associated antisera: studies with primed responding cells.

The inhibitory effects of HLA-D-associated antisera on stimulating lymphocytes when cultured together with allogeneic primed responding lymphocytes were investigated. The responding lymphocytes were primed for either the HLA-Dw2 or Dw3 determinants. The presence of HLA-D-associated (Ia-like) antibodies during the culture period specifically inhibited the stimulatory capability of lymphocytes possessing the HLA-D determinants with which the antisera were apparently reacting, as long as the stimulating cells carried the determinant for which the responding cells were primed. HLA-D-associated antisera of other specificities caused no decrease in the stimulatory capability. These antisera, therefore, apparently contain antibodies which are reactive with determinants closely associated or identical to the HLA-D determinants and may be human analogues to the mouse Ia antigens.

Binding Sites, Antibody↗
PubMed1977

Cell mediated lympholysis: CML. a microplate technique requiring few target cells and employing a new method of supernatant collection.

A micro method for the 51Cr release assay is described. Allogeneically induced cytotoxic lymphocytes are generated in micro mixed lymphocyte cultures in the wells of micro plates. Their cytotoxic capacity is assayed by adding 51Cr labelled PHA derived lymphoblasts directly into the micro cultures with no pooling or transfer of the cytotoxic effector cells being required. The 51Cr isotope released into the cell supernatants is collected by inserting a cellulose acetate absorption cartridge into each well. A glass fiber filter attached to the cartridge effectively separates the supernatant from the cellular elements. This system allows the simultaneous collection of the supernatant from 96 wells, and can be used with either adherent or non-adherent target cells.

Cells, Cultured↗
PubMed1977

Activation of human suppressor cells in mixed lymphocyte cultures.

Lymphocytes transferred from 6-day-old human mixed lymphocyte cultures (MLC) inhibited the response to allogeneic cells of freshly taken lymphocytes autologous to the transferred MLC-responding cells. No specificity for this cell-dependent suppression could be determined. The inhibitory effects of the suppressing cells could be abrogated by moderate X-ray irradiation and significantly reduced by pretreating them with an anti-Ia-like antiserum and complement. The human suppressor cell responsible for the observed effects therefore appears to carry determinants equivalent to the murine Ia alloantigens.

Complement System Proteins↗
PubMed1977

Stimulation of human lymphocytes by allogenic macrophages in vitro.

Macrophages were obtained from human peripheral blood by incubating mononuclear cells in plastic tissue culture flasks. After 1-14 days, the cells were used in mixed lymphocyte macrophage cultures. Macrophages could not, themselves, be stimulated to proliferation by allogenic cells, but stimulated allogenic lymphocytes. By the use of responding cells and stimulating macrophages from HLA-D homozygous individuals, the HLA-D determinants could be shown to be responsible for the stimulation of allogenic lymphocytes.

Cells, Cultured↗
PubMed1976

Typing for HLA-D determinants. comparison of typing results using homozygous stimulating cells and primed cultures.

Typing for HLA-D determinants, using primed lymphocyte typing (PLT), has been compared to the results obtained using conventional homozygous stimulating cell primary MLC tests. The HLA-Dwl, -Dw2 and -Dw3 specificities were studied. Preliminary results indicate that these two methods essentially type for the HLA-D determinants and that reproducible results can be obtained employing the PLT technique after 24 hours of incubation in the secondary cultures.

Epitopes↗
PubMed1975

The human mixed lymphocyte-endothelium culture interaction.

Cells separated from the wall of the umbilical cord vein by collagenase digestion could be identified as endothelial by their characteristic ultrastructure, their growth pattern in culture, and their microscopical morphology. These cells, both freshly explanted and after long-term culturing, were capable of stimulating allogeneic lymphocytes in vitro. Control experiments indicated that this stimulation was not attributable to contamination of the endothelial cell suspensions by foetal fibroblasts or passenger lymphocytes. The dose response characteristics and kinetics of the lymphoproliferative response using endothelial stimulating cells was similar to mixed lymphocyte cultures. Sera which were capable of inhibiting the mixed lymphocyte culture response were relatively ineffective in inhibiting the stimulation caused by endothelial cells.

Carbon Radioisotopes↗
PubMed1975

Lymphocyte activating alloantigens on human epidermal cells.

Human epidermal cells dissociated by repeated trypsinization of skin explants were stimulatory to allogeneic lymphocytes in mixed lymphocyte skin-cell cultures. After elimination of the lymphocytes proliferating in response to a particular allogeneic lymphocyte donor by a "hot" pulse of 3H-thymidine of high specific activity, the viable lymphocytes remaining in culture were still capable of responding to stimulation by epidermal cells from the same donor. The response towards allogeneic epidermal cells could only partially be eliminated by 3H-thymidine treatment. This treatment, however, also partially eliminated the secondary stimulation response towards lymphocytes from the epidermal cell donor used initially, but did not alter the response to lymphoid or epidermal cells from an unrelated third party donor. In addition, HL-A antisera, which specifically inhibited the stimulatory capacity of mitomycin-treated lymphocytes that carried the relevant HL-A antigen also inhibited the stimulatory properties of epidermal cells from the same donor.

Epitopes↗