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Biomedical subjects

H Herrmann

Publications and source records attributed to H Herrmann.

At least 127 records · Page 7Linked to original sources
PubMed1982

[Relevance of the maximal expiratory flow-volume curve in an epidemiological study].

In an epidemiological study a random sample (1,554 males aged 35 to 54 years) of the population of a hydrogen chloride air-polluted area was investigated by means of the modified MRC questionnaire and maximal expiratory flow-volume curves. The MEF-values were dependent on age and height. We stated no significant differences between potash miners and the remaining population. In contrast we found significant differences between smokers and non-smokers and also between persons with and without respiratory symptoms. Persons with bronchitis and chronic bronchitis most differed from reference groups in MEF 75 (flow at high lung volume). Comparing smokers with non-smokers MEF 25 attained the highest significance level. In our investigation the maximal expiratory flow-volume curve was only a little more sensitive than conventional spirometry.

Adult↗
PubMed1980

Incorporation of [1-14C]octadecanol into the lipids of Leishmania donovani.

After incubation of stationary phase Leishmania donovani with [1-14C]octadecanol, about 70% of the precursor was taken up within 3 hr. Wax esters and acyl moieties of glycerolipids contained most of the 14C-activity from 3 to 6 hr, because octadecanol was partly oxidized to stearate. Ether moieties were only weakly labeled. After 40 hr, 1-0-alkyl and 1-0-alk-1'-enyl diacylglycerols as well as 1-0-alkyl and 1-0-alk-1'-enyl-2-acyl-sn-glycero-3-phosphoethanolamines contained nearly all of the radioactivity. Most of the label in the neutral ether lipids was located in the alkyl ether side chain, whereas, in the phosphatidylethanolamine fraction, most of the label was found in the alkenyl ether side chain. Administration of 1-0-[1-14C]hexadecyl glycerol resulted in rapid labeling of the vinyl ether side chain of phosphatidylethanolamine plasmalogen (1 hr) increasing further at 2.5 hr. Most of the radioactivity in the alkoxy diacylglycerols was found in the 1-0-alkyl moiety.

Animals↗
PubMed1980

Synthesis of types I, III and AB2 collagen by chick tendon fibroblasts in vitro.

Tendons from 14--17-day-old chick embryos contain predominantly type I collagen and about 5% AB2 collagen; type III collagen is not detectable by biochemical methods, such as sodium dodecyl sulfate/polyacrylamide gel electrophoresis or cyanogen bromide pattern, but can be visualized by immunofluorescence staining with collagen-type-specific antibodies. Similarly, freshly dissociated tendon cells secrete only type I collagen into the culture medium but no significant amounts of type III collagen [Uitto, J., Lichtenstein, J. R., and Bauer, E. A. (1976) Biochemistry, 15, 4935--4942]. Transfer of tendon cells from chick embryos to monolayer conditions, however, initiated synthesis of type III collagen in about 10% of the cells within three days, as visualized by immunofluorescence staining. Secretion of type III collagen into the culture medium can also be detected by sodium dodecyl sulfate/polyacrylamide gel electrophoresis. With increasing number of passages the number of cells producing type III collagen reached levels of about 80% after the third passage, while 90% of all cells stained positively for type I collagen. This is reflected by an increase of production of type III collagen as determined by CM-cellulose chromatography. Using velocity sedimentation, the secretion of type III procollagen and of pN-collagen (carrying the amino-terminal extension only), into the culture medium of a second-passage tendon cell culture was detected. This study provides new evidence that the phenotype of cells may alter during transfer from the environment in vivo to conditions in vitro and that additional changes may occur with time in culture.

Animals↗
PubMed1979

[Chronic diseases of the respiratory tract (proceedings)].

On account of their increasing social-medical importance chronic diseases of the respiratory tract demand systematic measures for prevention and combat. The size of the problem is described on the basis of the results of epidemiologic investigations. Issuing from the risk degrees of the cardio-respiratory insufficiency (obstruction, hypoxaemia, pulmonary hypertension) care groups are proposed. The combat against tobacco smoking (primary prevention) and long-term treatment of the chronic obstructive bronchitis (tertiary prevention) stand in the foreground of the measures. The solution of this task demands a close cooperation with the basic medical care.

Adult↗
PubMed1979

[The value of functional rehabilitation of patients with clefts for their growth and development].

The optimal rehabilitation of patients with cleft lips and palates can be realized only in the form of complete rehabilitation by the joint efforts of the oral surgeon, the logopaedist, the orthodontist and the prosthodontist. Clinical observations and studies have shown that the following three findings cannot satisfy: - imperfect functional rehabilitation of the cleft lip, especially of the bilateral cleft lip; - considerable constriction of the vestibulum, which is also relatively often seen in bilateral cleft lips; and - surgically-induced considerable flattening of the palatine vault, which restricts the use of oral removable appliances.

Cleft Lip↗
PubMed1978

Evaluation of continuous labelling data by a practical mathematical method with application to a virus-induced sarcoma of the rat.

A simple mathematical function is established which renders the increase of the labelling index during continuous labelling of an exponentially growing tumor. By known values of proliferative fraction, mean cell cycle time and tumor doubling time, the mode and extent of cell loss is determined. Three models for different modes of cell loss are introduced: Model A for cell loss equally affecting proliferative and resting cells; Model B for cell loss predominantly affecting one of the two cell compartments; Model C for cell loss during mitosis. The range of validity of the model function is specified. Experimental data of a Polyoma virus-induced renal sarcoma of the rat provide an example of applicability of the theoretical model.

Animals↗
PubMed1978

[Differentiation of influenza virus antigens using enzyme immunoassay].

Reported is a microprocess of enzyme immune assay in which slipformed air pockets of polyvinylchloride (PVC), as used in the pharmaceutical industry, are used as carriers of antigens or antibody. Two methods, the anti-globulin and the double-antibody methods, are based on antibody which had been coupled with alkaline phosphatase. Tests in which various sub-types of influenza virus were used have shown the double-antibody method to be a sensitive technique which can be successfully used in the differentiation of envelope antigens.

Alkaline Phosphatase↗
PubMed1978

[Differentiation of matrix proteins of influenza A viruses using enzyme immunoassay].

Matrix protein is known as a type-specific structural protein of influenza viruses. An attempt has been made to find out whether or not strain-specific components could be detected from matrix protein, in addition to its type-specific antigen determinants. The technique of enzyme immune assay was chosen as the optional method to differentiate between matrix proteins of various influenza-A viruses. Antigen titration was undertaken of several matrix proteins, using two specific anti-matrix-protein sera in each case. Information regarding serological relationships between the tested matrix proteins of various influenza-A viruses was obtained from a quotient between the titres of one antigen, on the one hand, and the two anti-matrix-protein sera used in titration, on the other. Two matrix protein sub-types were established in the context of the influenza-A viruses tested. Sub-type M1 was attributable to older strains (A/PR/8 and A/FM/1), whereas the matrix protein of sub-type M2 was found to be present in more recent strains (A/Hongkong and A/Port Chalmers).

Antigens, Viral↗