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H Haug

Publications and source records attributed to H Haug.

At least 55 records · Page 3Linked to original sources
PubMed1989

The cerebral dominances: quantitative morphology of the human cerebral cortex.

The aim of the present investigation was to detect differences between the left and right hemispheres at a macroscopical level using morphometric procedures. The volume fraction and the surface area of the different brain structures have been calculated from the profile area by stereological procedures. The volume, the relative volume fraction, the folded surface area as well as the mean cortical thickness were evaluated by stereology. Although some constant differences in the arrangement of sulci and their depth can be observed at a descriptive level, the quantitative morphological comparison between both hemispheres however only showed slight differences. The results are discussed with regard to the differences published up to the present. The morphometric methods will be discussed in detail.

Adult↗
PubMed1988

New one-step enzyme immunoassay for free thyroxin.

This enzyme immunoassay for free thyroxin (FT4) involves simultaneous incubation of sample and thyroxin conjugated to horseradish peroxidase (EC 1.11.1.7) in antibody-coated tubes at room temperature. The test can be performed in 90 min. It is not influenced by variations in concentrations of thyroxin-binding globulin (TBG), thyroxin-binding prealbumin, or albumin. With increased concentrations of non-esterified fatty acids, the measured FT4 equivalent concentrations increase immediately, indicating displacement of endogenous thyroxin from its binding sites. FT4 values obtained with this assay for 110 samples, including sera with low albumin and high TBG as well as sera of patients treated with heparin, agreed well with those measured by a two-step radioimmunoassay and with the T4/TBG ratio. The measurable range of FT4 with our assay is 2.5 to 65 ng/L. Intra-assay CVs range from 3.4% to 2.2%, interassay CVs from 6.6% to 2.6% at concentrations of 9 to 45 ng of FT4 equivalent per liter.

Fatty Acids, Nonesterified↗
PubMed1988

[Free small intestine transplantation in plastic reconstruction of the oral cavity, pharynx and cervical esophagus. Report of experiences following 3 years of use].

Between August 1984 and August 1987 77 free intestinal grafts were transplanted in our Department of otorhinolaryngology using microvascular anastomoses. These reconstructions were necessary after tumour resections in the oral cavity, in the pharynx, and the cervical oesophagus. Patients, operative technique, perioperative treatment, and complications are described. The definition of oncologic results is still problematic. Only trends have been discovered. In conclusion, complications, functional results, and the advantages of free intestinal grafts are discussed.

Adenocarcinoma↗
PubMed1987

Brain sizes, surfaces, and neuronal sizes of the cortex cerebri: a stereological investigation of man and his variability and a comparison with some mammals (primates, whales, marsupials, insectivores, and one elephant).

This study deals with the stereological estimation of macroscopic sizes of brain and cortex, i.e., volume, surface, and folding, and of microscopic neuronal sizes, i.e., density, mean size, size distribution, and number of neurons. The results show that the degree of variability in man amounts to about 15%. A decrease in volume of the different gray structures can be observed in man after the age of 65 years. The surface, folding index, and length of convolution do not alter with aging. The comparison with mammals of various sizes allows the conclusion that there is a high correlation to brain size for nearly all macroscopic values. Man and elephant, however, have a cortical surface which is, in comparison with whales, relatively small. In contrast, whales have very small cortices compared with man. At the cytoarchitectonic level, the neuronal density has a correlation to brain size. Contrary to other mammals, the primates and man have a high fraction of small granular neurons, especially in layer 4. The assumption that the number of cortical neurons beneath a given surface area of cortex is the same in all mammals cannot be verified, especially in those with large brains. The allometric connection between brain size and parameters is not valid for all measurements (e.g., thickness of cortex, mean size of neurons, perikaryal size distribution, and glial density). Yet some other measurements are well correlated.

Animals↗
PubMed1987

Cloning and nucleotide sequence of heavy- and light-chain cDNAs from a creatine-kinase-specific monoclonal antibody.

Determination of creatine kinase isoenzymes by inhibition assay is a useful tool for the diagnosis and monitoring of myocardial infarction. We have established several mouse hybridoma lines secreting monoclonal antibodies with creatine kinase M-subunit inhibitory capacity. One of the monoclonal antibodies (MAK33) inhibits creatine kinase-MM by 80% without influencing the activity of creatine kinase-MB. A combination of two monoclonal antibodies increased the inhibition of creatine kinase MM up to 99.4%. Poly(A) + RNA of hybridoma cells secreting MAK33 was isolated and used for cloning cDNA of both heavy and light chains of this antibody. Full-length cDNA clones were obtained by hybridization with gamma 1 and kappa constant region cDNA probes. The complete nucleotide sequences from the variable regions including signal peptide and part of the 5'-untranslated regions have been determined.

Amino Acid Sequence↗
PubMed1986

Penicillin acylase from E. coli: unique gene-protein relation.

The nucleotide sequence of the gene (pac) coding for penicillin G acylase from E. coli ATCC 11105 was determined and correlated with the primary structure of the two constituent subunits of this enzyme. The pac gene open reading frame consists of four structural domains: Nucleotide positions 1-78 coding for a signal peptide, positions 79-705 coding for the alpha subunit, positions 706-867 coding for a spacer peptide, and positions 868-2538 coding for the beta subunit. Plasmids were constructed which direct the synthesis of a pac gene product lacking the signal peptide, and the synthesis of the alpha subunit or the beta subunit. The following results were obtained: The two dissimilar subunits are processing products of a single precursor polypeptide; the spacer peptide is removed during processing; the precursor polypeptide lacking the signal sequence is accumulated in the cytoplasm; it is not processed proteolytically in the cytoplasm and it does not display enzyme activity. Processing, therefore, requires translocation through the cytoplasmic membrane; processing follows a distinct sequential pathway in vitro.

Amidohydrolases↗
PubMed1986

History of neuromorphometry.

The history of morphometry begins in the middle of the last century. At the turn of the century impulses had decreased. The alterations arising during the preparation of the tissues were almost unknown. For example, the dogma of the loss of neurons during aging was conditioned by age-dependent histological shringage of the brain. Beginning in the thirties of this century new thoughts have given impulses to new morphometric investigation. In the neurosciences important impulses came from Bok. In 1961 Elias introduced the term "stereology" as a new interdisciplinary science in order to communicate the different developments. Since 1970 the computer and image analysis have brought a new dimension to morphometric research. This paper describes some neuromorphometrical developments. An increasing accuracy of results regarding density and amount of neurons can be estimated with facilitated counting procedures. A similar development can be observed for the estimation of neuronal sizes and their size distribution as well as for the volume-parts of the average perikaryon volume fraction (grey cell coefficient). The morphometry of dendritic tree and the ultrastructure of neuropil are briefly mentioned.

Animals↗