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Biomedical subjects

H Hatanaka

Publications and source records attributed to H Hatanaka.

At least 271 records · Page 15Linked to original sources
PubMed1986

Autonomic nervous function determined by changes of periflux blood flow in the aged.

The periflux blood flow (PBF) in fingers was determined by a periflux laser Doppler flowmeter (PLDF). The patterns of PBF responses to standing and Valsalva's maneuver were analyzed. The PBF responses in both loading tests were well correlated with the results of the conventional autonomic function test. It was, therefore, considered that our method would be useful for assessment of autonomic function. We practically investigated PBF responses in the two loading tests in three groups: young age group (10-39 years), middle age group (40-69 years) and old age group (70-89 years). The old age group showed significant decreases in both sympathetic and parasympathetic functions. It was indicated that this non-surgical and simple autonomic function test will be useful particularly in the elderly.

Adolescent↗
PubMed1986

Differential effects of nerve-growth factor and glioma-conditioned medium on neurons cultured from various regions of fetal rat central nervous system.

When nerve-growth factor (NGF) was added to 17-day fetal rat central nervous system (CNS) septal neurons in culture using a defined medium, choline acetyltransferase (ChAT) activity was greatly induced. However, glioma-conditioned medium (GCM), which is expected to contain cholinergic neurotrophic factor(s) different from NGF, did not affect the ChAT activity of the cultured septal neurons. On the contrary, ChAT activity of 19-day fetal rat hippocampal neurons in culture was increased by the addition of GCM but not by NGF. These phenomena were confirmed in septal and hippocampal neuronal cultures obtained from the same embryonic day, i.e., 18-day fetal rat. The NGF-mediated increase in ChAT activity of cultured septal neurons was culture-time dependent (2.3-fold increase after 3 and 3.5-fold increase after 6 days in culture) and NGF-dose dependent (the ED50 value was 0.8 ng/ml). The effect of NGF was completely abolished by the addition of specific anti-NGF antibodies. The differential effects of NGF and GCM on several other cultured cholinergic neurons from 17-day fetal rat spinal cord, striatum, brainstem and amygdala were measured. NGF tended to increase ChAT activities of cultured striatal and amygdala neurons but not cultured spinal cord and brainstem neurons. GCM increased ChAT activities in the latter two cultures, while having no effect on the former cultures. Although the extent of increase of NGF-mediated ChAT activities of cultured striatal and amygdala neurons were low, NGF-mediated increase of the striatal ChAT activity showed the pattern resembling that of cultured septal neurons as to time-course, dose-dependency and anti-NGF antibody sensitivity. NGF did not affect the tyrosine hydroxylase activity in the cultured brainstem catecholaminergic neurons.

Animals↗
PubMed1986

Increased fructose-lysine of nail protein and blood glucose control in diabetic patients.

Furosine, which was formed by acid hydrolysis of fructose-lysine, was determined and used as an indicator of glycosylated protein. The diabetic patients had significantly higher fructose-lysine levels in finger nails than healthy subjects [10.8 +/- 4.6% (mean +/- S.D.) vs 4.2 +/- 1.1%]. The best correlation was found between the fructose-lysine value and the fasting blood glucose level determined 3 to 5 months before sampling nails in diabetics. These results suggest that the furosine derived from fructose-lysine in finger nails may become an indicator of blood glucose control during the past 3 to 5 months in diabetics.

Adult↗
PubMed1986

Age-related acceleration of glycation of tissue proteins in rats.

The Maillard reaction on proteins is a nonenzymatic glycosylation that is now termed glycation and produces a ketoamine linkage on the protein. The extent of this glycation was estimated by determining furosine (epsilon-N-(2-furoylmethyl)-L-lysine), which is derived from glycated lysine residues. Glycation of rat sciatic nerve and aorta increased with advancing age for up to 50 weeks and the high degree of glycation was maintained thereafter. The level of hemoglobin did not change significantly after 14 weeks. These results suggest that tissue glycation may increase with aging and may be involved in the mechanism of aging.

Aging↗
PubMed1986

Hb Himeji or beta 140 (H18) Ala----Asp. A slightly unstable hemoglobin with increased beta N-terminal glycation.

A fast-moving abnormal hemoglobin found in a diabetic patient was identified as beta 140 (H18) Ala----Asp. It comprised about 40% of the total hemoglobin. The beta N-terminal glycation in the abnormal hemoglobin was estimated to be 3 times as much as that in Hb A in the same blood sample. The abnormal hemoglobin was slightly unstable. Oxygen affinity of the stripped hemoglobin was decreased, but that of red cells from the carrier was slightly higher than normal because of the reduced effect of 2,3-diphosphoglycerate.

Blood Protein Electrophoresis↗
PubMed1986

Boron distribution analysis by alpha-autoradiography.

The distribution of the boron-10 compound, Na2 10B12H11SH, which is now in clinical use for boron neutron capture therapy for brain tumors, was studied topographically and quantitatively in rats using neutron-induced alpha-autoradiography. Transplanted intracerebral tumors in Sprague-Dawley rats were used. In the normal brain, only a minute amount of 10B (less than 1 microgram 10B/cm3) was found in the brain parenchyma, except for the infundibulum and area postrema. Boron-10 accumulated in the brain tumors. The tumor-to-blood concentration ratio of 10B increased with time after injection and reached unity 12 hr after injection. The tumor concentration calculated at that time was 18 micrograms 10B/cm3. This study clearly shows that this 10B compound accumulates in the transplanted rat tumors in the brain and that tumor concentration and tumor-to-blood ratio of 10B can provide a sufficient condition for brain tumor treatment.

Animals↗
PubMed1985

Neuronal differentiation of Ca2+ channel by nerve growth factor.

The inhibitory effect of nicardipine, a potent Ca2+ channel blocker in muscular cells, on the Ca2+ channel of clonal rat pheochromocytoma cells (PC12h) and cultured rat adrenal medullary cells was studied during the neuronal differentiation mediated by nerve growth factor (NGF). Nicardipine at nM-order concentrations suppressed the high-K+-evoked, Ca2+-dependent release of preloaded [3H]norepinephrine from PC12h cells and adrenal medullary cells, whereas it scarcely inhibited the release from the cultured rat brainstem cells. The inhibitory actions of nicardipine on both PC12h and newborn rat adrenal medullary cells were significantly decreased after these cells were cultured in the presence of NGF. These results suggest that the changes in Ca2+ channel are accompanied by the neuronal differentiation mediated by NGF.

Adrenal Gland Neoplasms↗
PubMed1985

Increased fructose-lysine of hair protein in diabetic patients.

Fructose-lysine, which is formed by binding glucose to lysine, is changed by acid hydrolysis into furosine. Furosine derived from fructose-lysine of hair was determined by high-performance liquid chromatography according to the method of Schleicher et al. Furosine values were significantly higher in diabetic patients than in healthy subjects, and significantly correlated with the stable components of hemoglobin A1 (HbA1) values. These results suggest that furosine, like HbA1, may become an indicator of past blood glucose control at any time in diabetic patients and be useful in investigating diabetic complications on the level of tissue.

Adult↗
PubMed1985

A specific method of glycosylated hemoglobin using furosine measurement.

In order to get an accurately specific determination of glycosylated hemoglobin (GHb-f), the furosine assay method, which can detect epsilon-amino-lysine-bound glucose, was investigated. A good correlation was found between GHb-f values obtained by this method and HbA1 values by conventional ion exchange column chromatography. It was, therefore, considered that this technique would be a new useful method for determining glycosylated hemoglobin.

Diabetes Mellitus↗
PubMed1985

Evaluation of periflux blood flow measurement in diabetic patients with autonomic neuropathy.

The periflux blood flow (PBF) response pattern in the fingertips determined with a periflux laser Doppler flowmeter was analyzed in a standing test in which the subject was instructed to stand up from the sitting position and in Valsalva's maneuver in which respiration was held for 10 s at the maximum inspiration, and indicators such as reaction time (RT), orthostatic dysreaction rate (ODR) and PBF decreasing velocity (PDV) were established for determining autonomic abnormality. RT and ODR were significantly correlated with the change in blood pressure and pulse rate, respectively, in the standing test, and PDV was significantly correlated with beat-to-beat variation. Abnormalities were found in about half of the diabetics in the standing test and Valsalva's maneuver. These results suggested that the analysis of PBF changes in the standing and Valsalva's tests may be an easy and useful approach for clinical examination of the autonomic nervous system.

Adult↗
PubMed1985

Determination of carbamylated plasma protein and its clinical application to renal failure.

We determined carbamylated plasma protein (CPP) according to the method of Hunninghake in patients with renal failure and normal subjects. CPP values were significantly higher in patients with renal failure than in normal subjects. In patients with renal failure, a significant correlation was found between CPP levels and blood urea nitrogen. Thus, CPP appears to be a new indicator of the status of renal failure, irrespective of hemodialysis.

Blood Urea Nitrogen↗