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Biomedical subjects

H Hansen

Publications and source records attributed to H Hansen.

At least 199 records · Page 11Linked to original sources
PubMed1983

[Arterial circulation of the extrahepatic bile ducts].

The blood supply of the extrahepatic bile ducts was investigated in 15 human specimens. Human embryology explains the origin of the main blood supply in this area by the a. pancreaticoduodenalis posterior superior, the a. cystica and the a. hepatic propria. Other arteries are inconsistent and functionally unimportant. 2 or 3 marginal arteries are predominant in the arterial system surrounding the common bile duct. Surgery of the extrahepatic bile ducts should be performed with respect to this particular arterial network.

Arteries↗
PubMed1983

[Pseudocysts of the adrenal gland].

Diagnosis, clinic and therapy of adrenal pseudocysts are described by means of three cases. The three characteristic signs of this rare diseases are: 1. traumatic anamnesis, 2. radiating lumbal pains, 3. calcified, paravertebral cyst.

Adrenal Gland Diseases↗
PubMed1982

Effects of Hoechst 33342 on survival and growth of two tumor cell lines and on hematopoietically normal bone marrow cells.

Our objective in this investigation was to determine whether Hoechst 33342, which is widely recognized as a DNA specific fluorochrome for living cells, is in fact nontoxic and kinetically nonperturbing at dye concentrations required to achieve acceptable DNA distributions. Three cell types were tested: HeLa S-3; SK-DHL2, a human lymphoma cell line; and hematopoietically normal human bone marrow cells. In the third system, only the cloning efficiencies were determined. Results differed considerably for the different cell types. While HeLa cells yielded excellent DNA distributions and were almost completely resistant to the cytotoxic and cytokinetic effects of the dye, SK-DHL2 cells were highly sensitive to the fluorochrome even at dye concentrations which produced very poor DNA distributions. Human bone marrow cells were intermediate in their stainability and toxicity response, and acceptable DNA distributions could be obtained at the nontoxic dye concentration of 2.5 muM. Clearly, different cell types differ considerably with respect to their cytotoxic and kinetic responses to Hoechst 33342. In some cases it may not be possible to ensure adequate staining of the cells for flow cytometry without significantly altering their viability and/or proliferative behavior.

Benzimidazoles↗
PubMed1982

Leukemia and other cancers, anomalies and infections as causes of death in Down's syndrome in the United States during 1976.

All 995 persons with Down's syndrome who died in the United States during 1976 and whose death certificates listed Down's syndrome as the underlying or a contributing cause of death were identified. This allowed the underlying causes of death of 793 affected persons to be analysed and compared to deaths in the whole US population for that year. Mortality ratios provided evidence that the excess risk of leukemia mortality continues into adulthood and that deaths from other hematopoietic malignancies also occur excessively among Down's syndrome adults. Congenital anomalies of all kinds in infancy and congenital defects of the heart in infancy and later were also excessive. Respiratory tract infections and pneumonia showed persistently high ratios. Diabetes was raised only at ages 24 to 34 years. Ischemic heart disease, non-hematopoietic cancers, accidents, suicides and violence were under-represented among the causes of death. Methodological limitations of proportional mortality analysis are discussed.

Abnormalities, Multiple↗
PubMed1981

Utilization of prenatal genetic diagnosis in women 35 years of age and older in the United States, 1977 to 1978.

As a measure of access to and acceptability of prenatal chromosomal diagnosis among older gravidas, we determined the ratio of use of prenatal diagnosis among women 35 years of age and older in Alabama, California, Manhattan, and Nebraska for the period 1977-1978. Utilization ratios were higher in 1978. Overall, utilization ratios were between 6% and 28%, well below the adjusted rates of 40% to 50% found in certain United States and British localities. Urban women tended to have higher utilization ratios than had rural women, and white women had higher ratios than had black women. Ratios were extremely low for black and rural residents. The oldest women (those greater than or equal to 40 years), who were at fivefold greater risk than women 35 to 36 years of age, had less than a onefold increase in utilization over the latter groups. The vast majority of older gravidas initiated prenatal care sufficiently early in their pregnancies to receive prenatal diagnosis. Current program strategies need to ensure access to prenatal diagnosis, especially for women greater than or equal to 40 years of age, women who are black, and women who live in rural areas.

Adult↗
PubMed1981

Cloning of fresh lymphoma cells.

We attempted to induce in vivo clonal growth of neoplastic lymphoid cells from fresh specimens of involved tissue from 22 patients with no-Hodgkin lymphoma (NHL). Conditioned media derived from two human B-lymphocyte tissue culture lines were tested for their ability to promote colony growth. In addition, we compared the incidence of colony induction to the flash-3H-thymidine labeling index (LI) of the cells in the tissues cultured. Successful colony induction occurred in three-tenths of the cases of diffuse histiocytic lymphoma and one-half of the cases of nodular lymphoma. Cloning efficiencies were low, ranging from 0.0003 to 0.04%. There was no apparent relationship between successful instances of cloning and LI. We have confirmed the observations of Jones et al that lymphoid tumor cells can be cloned from tissues involved by NHL using a soft agar system. The possibility of using such systems for clinical predictive assays of chemotherapeutic drug toxicity toward tumor cells of NHL is discussed. We conclude that further refinements of the assays are desirable before they can be applied to widespread clinical use.

Clone Cells↗
PubMed1981

[Morphology of the lower esophagus after endoscopic varicosclerosation (author's transl)].

Submucous injection of 5 p.c. phenol in arachis oil in the lower esophagus can stop a bleeding from esophageal piles without considerable complications. Microscopical examinations on 14 human esophageal specimens demonstrate that phenol in oil as opposed to other sclerosing solutions produces only a mild, bland running inflammation in the tissue. Deep mucosal ulcerations, perforations of the wall, phlegmonous inflammations or mediastinitis could be observed. Nevertheless, under the intact esophageal mucosa a sufficient production of collagen fibres develops, which is effecting a varicosclerosation.

Adult↗
PubMed1981

Intrinsic factor, free of R proteins, can be prepared from mouse stomach and used in a ligand assay specific for "true" cobalamin.

A ligand assay specific for cobalamin that uses mouse stomach as the source of intrinsic factor has been developed. When mouse stomach extract incubated with radiocobalamin is fractionated by gel chromatography, the radioactive complex elutes as a single peak with apparent molecular weight of 54,900. Formation of the complex is greater than 98% inhibited by human anti-intrinsic factor antibody. When the equivalent of 10,000 pg/ml of cobinamide is added to serum, the apparent cobalamin concentration detected averages 8.5 pg/ml. Correlation with the Lactobacillus leichmannii microbiologic assay results in the regression equation y = 0.97x + 20. In six patients who had megaloblastic anemia the serum cobalamin by the mouse intrinsic factor ligand assay ranged from 0 to 9 pg/ml. Because the primary source of intrinsic factor is free of R proteins, there is no need for extensive purification of the extract. The assay is sensitive, precise, and accurate, and no more difficult to perform than other conventional ligand assay procedures.

Anemia, Megaloblastic↗
PubMed1981

Human hematopoietic tumors in nude mice.

Despite the difficulty in establishing human hematopoietic tumors in nude mice, four human lymphomas were successfully heterotransplanted and passaged serially in our laboratory. Additional immunosuppression with chemotherapy, whole-body radiation or splenectomy was not required for establishment of these tumors. All four of these tumors were of the non-Hodgkin's lymphoma type. In each case the tumors in the nude mice were histologically identical to the biopsy specimens from the patient in whom they were derived. Attempts to transplant tumor from 17 patients with Hodgkin's disease or 4 patients with immunoblastic lymphadenopathy were unsuccessful. Tumors from 2 patients with chronic myelogenous leukemia and 1 with hairy cell leukemia could be grown in nude mice conditioned with whole-body radiation or cytosine arabinoside, but these tumors could not be passaged to other nude mice. Cell surface markers were determined on the four serially passaged lymphomas. These surface markers were similar to the markers on the original tumors, even after long periods of mouse-to-mouse passage. In 1 patient with fevers, night sweats and mediastinal mass in whom a diagnosis had not been made after several biopsies, examination of tumor tissue that had been transplanted from the patient to the nude mouse clearly established the diagnosis of lymphoma.

Animals↗