Ultrastructure of the human myocardium in congenital and acquired heart defects.
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Biomedical subjects
Publications and source records attributed to H Grzybek.
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Tissue samples taken during surgery for therapeutic reasons from patients with acquired valvular heart disease (a.v.h.d.) were examined for collagen ultrastructure evaluation. The case material was obtained from 26 patients, and samples from the following organs were evaluated: palatine tonsils, mitral and aortic valves, heart auricle, skeletal muscle, periodontium and subcutaneous tissue. The examinations revealed the presence of general collagenopathy in a.v.h.d. The authors believe that examinations of the connective tissue ultrastructure may be useful and important in diagnostics and prophylaxis of a.v.h.d.
The study was carried out in 80 women with hyperthyroidism (40 with Graves-Basedow disease and 40 with hyperactive nodular goitre) and in 30 women with primary hypothyroidism. In the group with hyperthyroidism the total leucocyte count and the absolute count of neutrophils in peripheral blood were lower than in controls. In the group hypothyroidism the absolute count of monocytes was higher than in controls. In both studied groups ultrastructural changes were noted in the cytoplasm of neutrophils.
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The authors carried out experiments with application of transmission and scanning electron microscopy in the examinations of the cell sediment of the cerebrospinal fluid obtained by membrane filtration. The method made possible ultrastructural examination of fluids with low cell count. The observed cells had natural shapes and well preserved morphological details.
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