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Biomedical subjects

H Geldermann

Publications and source records attributed to H Geldermann.

72 records · Page 4Linked to original sources
PubMed1977

Inheritance of amylases in blood serum of cattle.

Serum amylase variants are demonstrated by means of starch gel and polyacrylamide gel electrophoresis. Phenotypes, allele frequencies, and segregation data for Am1 and Am2 in the cattle breed Deutsche Schwarzbunte are given. Demonstration of Am2 amylases was better in polyacrylamide gels and more isoenzymes were identified than in starch gels. The variants of Am1 amylases found in STAGE could not be reproduced in PAGE by means of the described methods. Both enzyme systems seem to be profoundly different in their molecular constitution and action. For the animals, these differences could be of advantage in the adaption to external influences.

Alleles↗
PubMed1976

Polymorphism of phosphoglucomutase in cattle leucocytes.

By menas of starch gel electrophoresis the polymorphism of the phospoglucomutase isozymes PGM1 has been investigated in cattle leucocytes. The phenotypess and allele frequencies of PGM1 are demonstrated in a series of 106 complete and 221 incomplete families of "Deutsche Schwarzbunte". The segregation of the 327 daughters' phenotypes agree with the formal genetic model of 2 codominant autosomal alleles. The observed heterozygous PGM1 type is more frequent in mating types and progenies as expected under equilibrium.

Alleles↗
PubMed1976

New protein polymorphisms in cattle.

Preparation techniques are outlined for blood samples from cattle, which permit rapid separation of several constant fractions within a large number of animals. Electrophoretical examination of the blood fractions, the methods for which are described, rendered possible the phenotyping of 5 new polymorphic protein systems. Furthermore the electrophoresis of erythrocyte carboanhydrase was tested. In about 550 cattle, including familial material of the breed 'Deutsche Schwarzbunte', the observed phenotypes, segregation data and allele frequencies of 7 polymorphisms, are described. We were able to find new polymorphisms of serum posttransferrins (Ptf), leucocytic proteins 1 and 2 (Leu1 and Leu2), and erythrocytic proteins 1 and 2 (Ery1, and Ery2). Moreover, data of carbonic anhydrase isoenzymes stained with protein dyes, as well as identified as esterases, are given.

Animals↗
PubMedDate not supplied

DNA fingerprinting in domestic animals.

DNAs of several species of domestic animals digested with the restriction endonucleases HinfI, AluI and HaeIII were hybridized with different synthetic probes. DNA fingerprint patterns were found in each investigated species by at least two of these probes. Furthermore, two probes gave sex-specific banding patterns in the chicken. Some applications of DNA fingerprinting in domestic animals are discussed.

Animals↗
PubMedDate not supplied

Genetic diversity of eleven European pig breeds.

A set of eleven pig breeds originating from six European countries, and including a small sample of wild pigs, was chosen for this study of genetic diversity. Diversity was evaluated on the basis of 18 microsatellite markers typed over a total of 483 DNA samples collected. Average breed heterozygosity varied from 0.35 to 0.60. Genotypic frequencies generally agreed with Hardy-Weinberg expectations, apart from the German Landrace and Schwäbisch-Hällisches breeds, which showed significantly reduced heterozygosity. Breed differentiation was significant as shown by the high among-breed fixation index (overall F(ST)= 0.27), and confirmed by the clustering based on the genetic distances between individuals, which grouped essentially all individuals in 11 clusters corresponding to the 11 breeds. The genetic distances between breeds were first used to construct phylogenetic trees. The trees indicated that a genetic drift model might explain the divergence of the two German breeds, but no reliable phylogeny could be inferred among the remaining breeds. The same distances were also used to measure the global diversity of the set of breeds considered, and to evaluate the marginal loss of diversity attached to each breed. In that respect, the French Basque breed appeared to be the most "unique" in the set considered. This study, which remains to be extended to a larger set of European breeds, indicates that using genetic distances between breeds of farm animals in a classical taxonomic approach may not give clear resolution, but points to their usefulness in a prospective evaluation of diversity.

Journal Article↗