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Biomedical subjects

H Fukushima

Publications and source records attributed to H Fukushima.

At least 253 records · Page 14Linked to original sources
PubMed1989

Cat-contaminated environmental substances lead to Yersinia pseudotuberculosis infection in children.

A 1-year-old boy was infected with Yersinia pseudotuberculosis serotypes 1b and 3, and his 3-year-old brother was infected with Y. pseudotuberculosis serotype 1b; both had drunk water from puddles in a garden of their housing district of Miyoshi City, Hiroshima Prefecture, Japan. The Y. pseudotuberculosis serotype 1b and 3 strains isolated from soil from the dried-up puddles and sand and feces from the sandbox proved to be from a stray cat. The restriction endonuclease patterns of the plasmid in each strain of Y. pseudotuberculosis serotypes 1b and 3 were identical. These data provide evidence for the transmission of Y. pseudotuberculosis through water, sand, and soil contaminated by feces from cats infected with this species.

Animals↗
PubMed1989

Special features of distribution of Yersinia pseudotuberculosis in Japan.

The incidence of Yersinia pseudotuberculosis isolation from humans, animals, water, and pork in Japan is presented on the basis of a review of the literature and serotyping of 633 strains referred to our laboratory. Most of the strains belonged to serogroup 4b, followed in frequency of isolation by serogroups 3, 1b, 5b, 5a, and the others, whereas serogroup 1a has not been detected. Although strains were isolated from 11 species of animals, none were isolated from birds.

Adolescent↗
PubMed1989

Effects of exercise training on biochemical and biomechanical properties of rat aorta.

The authors studied the effect of prolonged physical exercise on the mechanical properties of rat aorta in relation to the amounts and qualities of arterial connective tissue fibrous proteins. Twelve male rats were divided into two groups: 6 sedentary rats (S) and 6 training rats (T), which were forced to swim from nine weeks to twenty-five weeks of age. The ultimate tensile stress and the ultimate tensile extension ratio of ring specimens at the descending thoracic aorta were larger in T than in S (192.3 +/- 47.9 g/mm2, mean +/- SD, vs 147.8 +/- 26.0, p less than 0.05; 3.52 +/- 0.13 vs 3.18 +/- 0.27, p less than 0.05; respectively). The elasticity parameter, calculated by fitting stress-strain curves to exponential function in the stress level of 0-20 g/mm2, was lower in T than in S (1.79 +/- 0.15 vs 2.13 +/- 0.24, p less than 0.01). The contents of elastin (alkali-insoluble elastin preparation) and collagen were higher in T than in S (0.44 +/- 0.11 g/g dry aorta vs 0.30 +/- 0.06, p less than 0.05; 0.15 +/- 0.04 g/g dry aorta vs 0.11 +/- 0.04, NS, respectively). Although the aortic calcium content did not significantly change in the training rats (T 1.17 +/- 0.23 mg/g dry aorta, S 0.95 +/- 0.34), the content of calcium in elastin was lower in T than in S (1.75 +/- 0.51 mg/g dry elastin vs 2.63 +/- 1.00, p less than 0.05).(ABSTRACT TRUNCATED AT 250 WORDS)

Amino Acids↗
PubMed1989

[Relationship between clinical findings and subgingival microbial flora in periodontitis (2)].

The purpose of this study was to examine the relationship between clinical findings and subgingival relationship between clinical findings and subgingival microbial flora in periodontitis at the first medical examination and after initial preparation. The results obtained were as follows: 1. Clinical findings with the exception of plaque index showed improvement after initial preparation in comparison with the first medical examination. 2. In phase contrast microscopy, both total bacteria and incidence of spirochetes and motile rods decreased after initial preparation in comparison with the first medical examination. 3. Clinical findings with the exception of plaque index were related to the total bacteria and proportional distribution of spirochetes and motile rods in periodontal pockets, observed in phase contrast microscopy. 4. Total bacteria and proportional distribution of black-pigmented Bacteroides in periodontal pockets decreased after initial preparation in comparison with the first medical examination.

Dental Prophylaxis↗
PubMed1989

[Relationship between clinical findings and subgingival microbial flora in periodontitis (1)].

The purpose of this study was to examine the relationship between clinical findings and subgingival microbial flora in periodontitis. The results obtained were as follows: 1. In a phase-contrast microscopic study, no correlation was found between the clinical findings, total bacteria or proportional distribution of spirochetes or motile rods in the periodontal pocket. 2. Anaerobic incubation revealed no correlation between clinical findings, total bacteria or proportional distribution of black-pigmented Bacteroides in the periodontal pocket.

Humans↗
PubMed1989

Strain difference and mode of inheritance of the susceptibility to passive cutaneous anaphylaxis mediated by allogeneic IgE antibody in the mouse.

The strain difference and mode of inheritance of the susceptibility to PCA (passive cutaneous anaphylaxis) were studied in mice. No marked strain differences were found in the susceptibility to PCA mediated by allogeneic IgG1 monoclonal and xenogeneic IgG antibodies, although BALB/c Crj mice were somewhat less susceptible than the other strains. On the other hand, the susceptibility to PCA mediated by allogeneic IgE monoclonal antibodies differed greatly according to the strain; SJL/J, A.SW/J and DS/Shi mice being highly susceptible and C57BL/6JShi, BALB/cCrj, DBA/2Crj, C3H/HeShi and CBA/JNCrj mice insusceptible. Based on the marked difference between DS/Shi and C3H/HeShi strains, the mode of inheritance of the susceptibility to IgE PCA was studied using their F1, F2 and F1 x parent backcross offspring. All the F1 hybrids by reciprocal crosses between these two strains and F1 x DS backcross mice were highly susceptible like DS/Shi mice. Backcross mice between F1 and C3H/HeShi showed approximately 1:1 segregation into the susceptible and insusceptible. Segregation was also observed in the F2 generation; 32% being insusceptible. These results suggest that the high susceptibility is a dominant phenotype controlled mainly by a single gene on an autosomal chromosome; this was statistically supported by tests of the segregation ratios obtained. However, in the PCA-positive recipients of F1 x C3H backcross and F2 generations, dye leakage into the skin was tended to be diffuse and less compact than in DS/Shi, F1 and F1 x DS backcross mice. This suggests the presence of some additional genes which modify the expression of the main gene.

Animals↗
PubMed1989

Flow cytometric analysis of the Rh1 (Rho, D) antigen activity on red cells: various Rh blood group phenotypes including Du variants.

Rh1 (Rho, D) antigen activity has been analyzed by the use of the indirect immunofluorescence flow cytometry (FCM), and the Rh blood group genotypes were able to be successfully determined from the intensity of fluorescence detected in flow cytometry using the anti-D IgG that was fractionated in a Protein A Sepharose CL-4B column as the primary antibody. The relative amount of the fluorescein isothiocyanate (FITC) bound to the D (R1R1, CDe/CDe), the high grade Du (R2r',cDE/Cde), the low grade Du (K1r, CDue/cde), and the d (rr, cde/cde) red cells was estimated from the mean fluorescent intensity. The FITC-binding activity of the high grade Du and low grade Du was 83% and 21% that of D. The antigen-antibody complex density profile was analyzed by using the FITC-conjugated protein-A in place of the second antibody. Compared with the found results using anti-human globulin as the second antibody, this method was less sensitive but it still was able to demonstrate the different degrees of fluorescence according to the Rh genotypes. The present FCM method is both simple and useful for (1) measuring the relative amount of antigens, (2) for detecting the dosage effect and (3) for deferminins the blood group genotypes.

Erythrocytes↗
PubMed1989

[Gallium-67 imaging in patients with myocarditis in childhood and youth].

Gallium-67 (Ga-67) myocardial imaging was performed in 19 patients (1 month to 21 years of age) with proven or suspected myocarditis. The anterior images, 48 hrs after the intravenous administration of 0.5-2.0 mCi Ga-67 citrate, were analysed using a computer. Regions of interest were set on the heart, lungs and background, excluding the sternum and vertebrae. The uptake of Ga-67 was evaluated quantitatively by the ratio of the average count of the heart to the average count of the lung (H/L). When the H/L value was higher than 130% the cardiac uptake of Ga-67 was designated positive. Histological examination was performed in 11 patients who underwent right endomyocardial biopsy. The patients were categorized according to their clinical findings. Group 1 consisted of 11 patients with clinical symptoms of dilated cardiomyopathy, six of whom had arrhythmias or elevated serum CPK-MB levels (group Ia) in the acute stage of the disease, and the remaining five had neither (group Ib). Group 2 consisted of eight patients with dysrhythmias but without heart failure. Four had elevated serum CPK-MB levels (group IIa), but the remaining four did not (group IIb). All patients in the group Ia had positive Ga-67 uptakes, and three patients in the group Ib had negative uptakes. The H/L values were significantly higher in the group Ia than in the group Ib (p less than 0.02).(ABSTRACT TRUNCATED AT 250 WORDS)

Adolescent↗
PubMed1989

[A cases of isoniazid-induced lupus].

A case of isoniazid (INH)-induced lupus occurring in a 62-year-old man is presented. He visited our hospital in May 1986 and a cavitary lesion was found in the right upper lobe on a chest roentgenogram. He had no previous history of treatment with antituberculotic agents. Though acid-fast bacilli were not found in his sputum, pulmonary tuberculosis was strongly suspected and INH, rifampicin and ethanbutol were administered. Four days after starting the treatment, minimal left pleural effusion was seen on chest X-ray film. Three months later he began to complain polyarthralgia in his digital joints. In a pleural effusion many lymphocytes were found; and the antinuclear antibody (ANA), the anti-extractable nuclear antigens (ENA) antibody, and the RNase resistant anti-ENA antibody were positive, and their titres were 20x, 1000x and 1000x, respectively, and the immune complex (IC) was 16.0 micrograms/ml (LT5). In blood serum, the ANA test the anti-ENA antibody and the RNase resistant anti-ENA antibody were positive with titres 40x, 640x and 640x respectively; and the IC was 14.0 micrograms/ml, and the RA test was positive. The improvement of clinical findings and disappearance of auto-antibodies seen after stopping INH confirmed the diagnosis as INH-induced lupus.

Antibodies, Antinuclear↗
PubMed1988

[Inhibitory effects of somatostatin analog (SMS 201-995) on pancreatic hormones in patients with malignant islet-cell carcinoma].

Acute effects of somatostatin analog (SMS 201-995) on pancreatic hormones were studied in two patients with malignant islet-cell carcinoma. Before and after subcutaneous injection of somatostatin with a doses of 50 micrograms, blood glucose (BG), serum growth hormone (hGH), C-peptide immunoreactivity (CPR), plasma immunoreactive glucagon (IRG) and gastrin were assayed, and changes in elution patterns of IRG and gastrin were also analyzed on Bio-Gel P-30 column chromatography. In Patient 1 with glucagonoma syndrome and hypergastrinemia, a prompt and remarkable decrease in plasma IRG and gastrin was observed after the injection of SMS 201-995 in association with a decrease in blood glucose, and then IRG and gastrin increased gradually. The suppressive effect continued for at least 6 hours. On gel filtration of the plasma obtained before the injection of the analog, three major peaks, greater than 20000, 9000 and 3500 molecular-weight (mol wt) fractions, were seen in IRG fraction. The decrease in plasma IRG observed at 1 hour after the injection was mainly due to a marked decrease in the 3500 molecular weight fraction. In addition, a slight decrease in the 9000 mol wt fraction was seen. At 4 hours after the injection, the 3500 mol wt peak returned to the previous level, while the 9000 mol wt peak decreased further. On the other hand, the gastrin elution pattern of plasma obtained before the injection revealed three major gastrin peaks, greater than 20000, 7000 and 5000 mol wt fraction. The changes in the gastrin elution pattern after the injection were similar to those of the IRG elution pattern. In Patient 2 with Zollinger-Ellison's syndrome, the plasma gastrin level decreased gradually for 5 hours after the injection. On gel filtration of the plasma obtained before the injection, two major gastrin peaks, 7000 and 5000 mol wt fraction, of which the large-molecular fraction was more prominent than the small-molecular fraction, were observed. After the injection, a marked decrease in the small-molecular fraction and a gradual decrease in the large-molecular fraction were observed for 4 hours, accompanied by a decrease in plasma gastrin. At 7 hours after the injection, the smaller fraction was augmented again. The serum CPR and hGH was slightly suppressed after the injection in both patients. The adverse effects of slight nausea and vomiting were noticed only in Patient 1.(ABSTRACT TRUNCATED AT 400 WORDS)

Adenoma, Islet Cell↗
PubMed1988

Detection of ABO blood group-active glycolipids extracted from red cell membrane and heat hematoma using TLC-immunostaining.

Glycolipids extracted from groups A, B, and O erythrocytes were developed on thin-layer plates; their ABO blood group antigenicities were detected by immunostaining method using avidin-biotin-complex (ABC). Among series of glycolipids of different flow rates, antigen-specific staining was observed in five bands from group A1 erythrocytes, four bands from group B, and two bands from group O. Monoclonal anti-A, -B, and -H antibodies specifically stained glycolipids from A1, B, and O erythrocytes, respectively. ABO blood grouping was possible from 5 g of epidural heat hematoma of a charred body by this method. ABC immunostaining on thin-layer chromatography is a useful and reliable method for ABO blood grouping in forensic practice.

ABO Blood-Group System↗
PubMed1988

Effect of alpha 2-adrenoceptor antagonist on platelet activation during insulin-induced hypoglycaemia in type 2 (non-insulin-dependent) diabetes mellitus.

The role of epinephrine in platelet activation and the effect of an alpha 2-adrenoceptor antagonist, midaglizole, during insulin-induced hypoglycaemia in Type 2 (non-insulin-dependent) diabetes mellitus were examined. The action of midaglizole as a platelet alpha 2-antagonist was confirmed by in vitro studies using platelet-rich plasma and washed platelet suspension. Hypoglycaemia was induced by a bolus injection of short-acting insulin in 24 diabetic patients. They were divided into two groups, a control group (n = 12) and an alpha 2-group (n = 12), and midaglizole was administered orally 60 min before insulin injection in the latter. Blood glucose and plasma C-peptide levels were significantly decreased (p less than 0.005) by insulin injection in both groups. Counter-regulatory hormones, including epinephrine, and arginine vasopressin were similarly increased at the hypoglycaemic nadir compared with the levels at 0 min in both groups. Plasma beta-thromboglobulin was increased at the hypoglycaemic nadir (165.5 +/- 12.6 ng/ml) compared with the level at 0 min (121.0 +/- 11.5, p less than 0.005) in the control group, whereas no significant increase was demonstrated in the alpha 2-group. These results suggest that plasma epinephrine plays an important role in platelet activation during hypoglycaemia in Type 2 diabetes mellitus, and that the platelet activation is prevented by alpha 2-adrenoceptor antagonist.

Arginine Vasopressin↗
PubMed1988

Ultrasound diagnosis of ureteral stones: its usefulness with subsequent excretory urography.

The diagnostic accuracy of ultrasound scanning and excretory urography was compared in 157 patients with ureteral stones. Over-all, the diagnostic accuracy rates were 78.3 per cent for ultrasound scanning and 81.5 per cent for excretory urography. These rates were 83.2 and 85.0 per cent, respectively, in 107 cases of upper ureteral calculi, and 68.0 and 74.0 per cent, respectively, in 50 cases of lower ureteral stones. When the studies were combined the diagnostic accuracy rates increased to 98.1, 94.0 and 96.8 per cent for upper, lower and all stones, respectively. Ultrasound scanning is useful in the diagnosis of ureteral stones, especially in patients with ureteral colic. However, ultrasound combined with subsequent excretory urography is the most reliable method for the diagnosis of ureteral calculi. Bladder filling is a useful diagnostic aid for ultrasound scanning of lower ureteral calculi.

Adult↗
PubMed1988

Virulence of Yersinia pseudotuberculosis isolated from pork and from the throats of swine.

Yersinia pseudotuberculosis was isolated from retail pork and from healthy swine throats. These wild-type strains and their representative cured isogenic strains were tested for the presence of plasmids and several virulence factors, and these characteristics were compared with those of virulent strains from humans. Two pork isolates (serotype IVB) and four swine isolates (serotypes IIB, IIC, III, and IVB) harbored a 42- to 48-megadalton plasmid which had similar fragmentation patterns resulting from digestion with restriction endonuclease. These six strains were lethal for mice via oral challenge and were positive in autoagglutination and calcium dependency tests. They also invaded HeLa cells and induced cytotoxicity. Histopathological examination and indirect fluorescent-antibody staining provided definite evidence of the pathogenicity of these strains when tissue sections from orally infected mice were used. The virulence factors of wild-type pork and swine isolates with the 42- to 48-megadalton plasmid were identical to those of two human isolates (serotypes IVB and VB). Hence, these pork and swine isolates should be considered potentially pathogenic for humans. The finding suggests that retail pork and swine may play an important role in the epidemiology of human infections caused by Y. pseudotuberculosis.

Agglutination Tests↗
PubMed1988

Yersinia pseudotuberculosis infection contracted through water contaminated by a wild animal.

We performed epidemiological studies on Yersinia pseudotuberculosis in one valley where a 3-year-old boy had been infected with Y. pseudotuberculosis serotype 4b in December 1982. Y. pseudotuberculosis serotype 4b was isolated from a water sample derived from a mountain stream from which the boy had drunk and from 1 of 41 rats trapped in the upper part of this stream in December 1986. The restriction endonuclease patterns of the plasmids in these isolates showed the rat and patient isolates to be identical but distinct from the water isolate. These data suggest the potential for transmission of Y. pseudotuberculosis through water contaminated by nondomesticated animals carrying this species.

Animals↗
PubMed1988

Lethal shock in partially hepatectomized rats administered tumor necrosis serum.

A minute dose of bacterial endotoxin is known to cause lethal shock in BCG (Bacillus Calmette Guérin)-sensitized mice and rats. To gain insight into the mechanism of this hypersensitivity to endotoxin, serum (tumor necrosis serum: TNS) was prepared from BCG-sensitized Lewis rats following endotoxin challenge and injected intravenously into Lewis rats 2 days after their partial hepatectomy. TNS injection caused lethal shock in Hpx (partially hepatectomized) rats but not in normally fed, fasted, or sham-operated rats. Hpx rats survived injection of serum prepared by either BCG sensitization or endotoxin challenge alone. Biochemical and histological examination of the Hpx rats injected with TNS indicated that profound hypoglycemia, hepatic and renal injury, and dysfunction of the coagulation system accompanied by hemorrhage were involved in the lethal shock. These experiments also suggested that serum component(s), probably monokine(s) derived from activated macrophages, might participate in the endotoxin shock in BCG-sensitized rats.

Animals↗