Search PubMed⌕ Search

Biomedical subjects

H Fukui

Publications and source records attributed to H Fukui.

At least 523 records · Page 29Linked to original sources
PubMed1981

In vivo effects of anti-asialo GM1. I. Reduction of NK activity and enhancement of transplanted tumor growth in nude mice.

Intravenous injection of anti-asialo GM1, which has been shown to eliminate natural killer (NK) activity in vitro in the presence of complement, completely abolished NK activity against lymphoma cell line (YAC-1) in spleen cells from athymic nude mice as well as from conventional mice. An immunofluorescence study revealed a decreased number of asialo GM1 positive cells in the spleens of mice injected with anti-asialo GM1 than in those of mice injected with normal rabbit serum. In the nude mice with reduced NK activity, incidence of tumor take and the growth were enhanced when syngeneic (RL male-1), and allogeneic (YAC-1) tumors and human tumors were transplanted subcutaneously. These results strongly suggest that NK cells play an important role in transplanted-tumor growth in vivo.

Animals↗
PubMed1980

The effects of protease inhibitors on histidine decarboxylase activities and assay of enzyme in various rat tissues.

Histidine decarboxylase (L-histidine carboxy-lyase, EC 4.1.1.22) of an extract of rat stomach was inactivated by a pancreatic extract. This inactivation was prevented by the protease inhibitors leupeptin, antipan, chymostatin, pepstatin, Trasylol and phenylmethanesulfonyl fluoride. Leupeptin, antipain, chymostatin and pepstatin together and phenylmethanesulfonyl fluoride alone prevented complete inactivation of the enzyme, while Trasylol had a weak protective effect. The inactivation and protection of histidine decarboxylase purified from whole fetal rats were similar to those of the stomach enzyme: both enzymes were strongly inactivated by trypsin and chymotrypsin, but not by elatase or carboxypeptidase Y. The histidine decarboxylase activities of various rat tissues were assayed in the presence of protease inhibitors: activity was highest in mast cells followed by the whole bodies of fetal rats and the stomach, while the activities were lower in decreasing order in the brain, spleen, lung and liver. Heart and kidney had no activity.

Animals↗
PubMed1980

Effectiveness of hardening films on die stone.

1. Cyanoacrylate resins and resinous die hardeners effectively improved the hardness and abrasion resistance of stone dies. 2. Application of liquid resins followed by blotting and/or blowing with compressed air produced an improved surface without clinically significant dimensional change. The extent of film buildup was qualitatively related to the viscosity of the resin. 3. Application of multiple coats of resin or simply soaking the dies in resin without further blotting or other mode of removal of the excess produced a thick film which can obliterate surface detail.

Calcium Sulfate↗
PubMed1980

Patterns of factor-VIII related antigen on crossed immunoelectrophoresis and large pore polyacrylamide gel-crossed immunoelectrophoresis in von Willebrand's disease.

Plasma samples from patients with various types of von Willebrand's disease were subdivided into six patterns according to the electrophoretic mobility and shape of VIIIR: Ag on crossed immunoelectrophoresis (CIE): pattern 1 no precipitation are, pattern 2 normal mobility with low arc, arc, pattern 3 intermediate mobility with low arc, pattern 4 faster anodal mobility with low arc, pattern 5 normal mobility with normal are height, pattern 6 faster anodal mobility with normal are height. Of 62 patients, 14 had pattern 1, 6 pattern 2, 16 pattern 3, 12 pattern 4, 9 pattern 5, and 5 pattern 6. Large pore polyacrylamide-agarose gel-crossed immunoelectrophoresis (PAAG-CIE) of crude factor VIII fraction from cryoprecipitate revealed no arcs in patients with pattern 1, three arcs of reduced height in the patients with patterns 2 and 3, four arcs very similar to normal control in patients with pattern 5, and two arcs with fast anodal migration in the patients with patterns 4 and 6. Crude factor VIII fractions from normal cryosupernatant showed one low fast anodally migrating arc corresponding to the fourth arc of normal cryoprecipitate. No peak was seen in patients with pattern 1, and one low fast anodal arc similar to normal control was present in the patients with patterns 2, 3, 4, 5 and 6.

Antigens↗
PubMed1979

Factor VIII procoagulant activity, factor VIII related antigen and von Willebrand factor in newborn cord blood.

The mean level of factor VIII procoagulant acitivity (VIII:C) and factor VIII related antigen (VIIIR:AG) was normal in 100 newborn cord plasmas, whereas that of von Willebrand factor (VIIIR:WF) activity was slightly lower than normal. On crossed immunoelectrophoresis, 20 of 50 newborn infants had an increased anodal mobility of VIIIR:AG. When the cord plasma showing an abnormal electrophoretic pattern was mixed with normal plasma, two precipitation peaks with a broad base were found. Similar mixing experiments with the abnormal cord plasma and plasma from a patient with atypical von Willebrand's disease did not normalize the electrophoretic mobility of VIIIR:AG. Gel filtration of the cord plasma with an abnormal electrophoretic pattern of VIII:AG, showed that the three activities were all detected at the position corresponding to a molecular weight of about 800 000. The results suggest the presence of qualitative abnormalities of the factor VIII molecule in half of full-term newborn cord plasma.

Adult↗