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Biomedical subjects

H Fujimoto

Publications and source records attributed to H Fujimoto.

At least 217 records · Page 12Linked to original sources
PubMed1989

[Hepatocellular carcinoma associated with schistosomiasis japonica; CT and angiographic features].

One-hundred and fourteen cases of hepatocellular carcinoma (HCC) which underwent computed tomography (CT) and angiography at the University Hospital of Yamanashi were reviewed. These included 24 cases of HCC associated with hepatic schistosomiasis japonica. Compared with the other cases, radiological features of HCC with schistosomiasis japonica were clarified. Patients with multinodular HCC were most common (13/24), followed by those with single nodular HCC (7/24), while those with massive or diffuse HCC were few (4/24). In patients with marked CT changes (grade III or IV) of hepatic schistosomiasis japonica, most of HCC nodules were disclosed as a mass with homogenous hypodensity surrounded by "shell-like calcifications". Obstruction of the portal vein or its branches due to tumor thrombus was rarely noted (4/24). These features are presumably attributed to periportal fibrosis which is characteristic of heptic schistosomiasis japonica. Because of these features, it is expected that hepatocellular carcinomas with schistosomiasis japonica can be treated by transcatheter arterial embolization more effectively than those without schistosomiasis japonica.

Aged↗
PubMed1989

[Production of fibroblast chemotactic factors from root canal bacteria].

It is well known that periapical pathosis is one of endogenous infections caused by indigenous bacteria in the oral cavity. Therefore, interaction of host and parasite factors affect the progress of the lesion. In host factors, fibroblasts migrate chemotactically, proliferate and constitute new connective tissues at a late stage of the inflammation process. All of chemoattractants for fibroblasts previously reported are derived from the host. In this study, fibroblast chemotactic activities in bacteria isolated from chronic periapical pathosis cases were examined. Fibroblast chemotactic activity was measured by the membrane filter method using cultured guinea pig dermal fibroblasts. Fibroblast migration was activated by bacterial supernatants of 4 species among 45 species tested. This indicates the possibility that these bacterial factors as well as host derivatives such as fibronectin, lymphokine, collagen-, elastin- and platelet-derived factors, may exert an influence on the process of periapical pathosis. The supernatant from Succinivibrio dextrinosolvens showed the most intensive chemotactic activity, which were separated into two fractions by Sephacryl S-300. The active fraction having a lower molecular weight (Mw. ca. 280K) did not absorb on DEAE-Sepharose CL-6B and this activity was resistant to heat and proteolytic enzymes.

Chemotactic Factors↗
PubMed1989

[Allogeneic bone marrow transplantation after the treatment of alpha-IFN and high-dose SNMC in two cases of acute myeloblastic leukemia with post-transfusional non-A, non-B hepatitis].

Two patients of acute myeloblastic leukemia (M2) with post-transfusional hepatitis (non-A, non-B) were treated with alpha-IFN and high-dose SNMC before allogeneic bone marrow transplantation. Bone marrow transplantation from HLA identical and MLR negative sibling donor was carried out when their hepatic functions were almost normalized. In the early phase after bone marrow transplantation, the hepatic function in both two cases has been stable, thus indicating that this treatment should be tried for reducing hepatic dysfunction and for safety bone marrow transplantation.

Adult↗
PubMed1989

[Alloreactivity and mitogen-induced responses in allogeneic murine bone marrow chimeras].

Alloreactive mixed lymphocyte reaction (MLR), mitogen-induced response (MR), and suppressor cells against these responses in murine bone marrow chimeras were examined, to clarify the mechanisms of immunological tolerance and immunodeficiency after bone marrow transplantation (BMT). Between 35 and 70 days after BMT, there was no response of spleen cells from allogeneic chimeras against host (C3H/He) and donor (BALB/c) cells, although responses against third party (C57BL/6) cells were detected, thus indicating that these allogeneic chimeras were immunologically tolerant. The activity of suppressor cells against alloreactive responses was increased 35 to 55 days after BMT, so that these suppressor cells appeared to be related to immunological tolerance. Some of the suppressor cells against alloreactive responses were Thy1+. Among them some were Lyt1+ or Lyt2+, and others were Lyt1+2+. Plastic dish non-adherent cells had slightly weaker suppressor activity than adherent cells. Proliferative responses to Con A, PHA, and PWM were decreased 13 to 15 days after BMT, and gradually increased. The responses to LPS differed from those to the former three mitogens, showing an enhanced response 21 to 25 days after BMT. The increased response to LPS did not appear to be simply due to the increased number of non-T cells in the spleen of allogeneic chimeras. The alloantigen specific suppressor cells may play an important role in the induction and maintenance of immunological tolerance, while the alloantigen nonspecific suppressor cells and suppressor cells against MRs may be related to immunodeficiency after BMT.

Animals↗
PubMed1989

[Transurethral unroofing of the müllerian duct cyst: a case report].

Management of Müllerian duct cyst depends on the size and symptomatology. Here we report a case of recurrent Müllerian duct cyst which was treated successfully by transurethral approach. A 57-year-old man was first admitted because of dysuria in 1981. Under the diagnosis of Müllerian duct cyst, he underwent resection of the cyst by suprapubic retrovesical approach that resulted in incomplete extirpation. In 1982, he was readmitted for recurrence of the cyst. Excision by transsacral approach was unsuccessful because of severe adhesion. He was followed by periodic puncture of the cyst and alcohol instillation. In 1988, he was hospitalized because of persistent urinary symptoms. Since open surgical procedure was thought to be difficult, it was replaced by transurethral management. A cyst bulging into the trigone was found with a cystoscope and the bladder wall over the cyst was resected transurethrally until the cyst was entered. A 2 cm opening between the bladder and the cyst was created with a resectoscope. Follow up CT scan two months later demonstrated sufficient communication between the bladder and the cyst. The patient voided well with no discomfort. We believe that transurethral unroofing of the cyst provides a safe treatment in selected cases.

Cysts↗
PubMed1989

[N-isopropyl-p-[123I]iodoamphetamine early dynamic image with ring-type emission CT in cerebrovascular disease].

N-isopropyl-p-[123I]iodoamphetamine (IMP) SPECT was done in 14 patients with cerebrovascular disease. Immediately after injection of 3 mCi IMP, sequential 2 minutes SPECT images (dynamic images) were obtained for 15 minutes using a circular detector array emission tomographic system (Shimadzu SET-030W). Then standard SPECT images were obtained at 30 minutes (early images) and 5 hours (delayed images) using a rotating scintillation camera ECT system (GCA-601E, Toshiba). Two patients out of 14 studies showed discrepancy of the findings between dynamic images and early images; dynamic images revealed low uptake area though early images showed no defect. Possible mechanism of this phenomenon might be due to so called redistribution phenomenon occurred during obtaining early images. In conclusion, early dynamic images is useful in cases with slightly decreased cerebral blood flow.

Adult↗
PubMed1989

[Two cases of adrenal cysts--the meaning of imaging in diagnosing adrenal cysts].

Two cases of adrenal cysts are reported. The first case is of a left adrenal cyst in a 19-year-old woman and was incidentally discovered by computed tomography (CT). Its margin was clear, its contents was homogeneous and its signal indicated water contents on magnetic resonance imaging (MRI). It was punctured and is now followed up by CT. The second case is of a left adrenal cyst in a 62-year-old woman, which also happened to be discovered by CT. Its contents was heterogeneous by MRI. It was removed and proved to be a lymphangiomatous cyst. The conclusion is that MRI will be useful in the differential diagnosis of adrenal cysts.

Adrenal Gland Diseases↗
PubMed1988

[Distribution of neurotensin-like immunoreactivity (NTLI) and neurotensin receptor in the rat brain and effects of various drugs on NTLI concentration and NT receptor in the rat cerebral cortex].

A radioimmunoassay for neurotensin(NT) and binding assay for NT receptor have been developed to determine neurotensin-like immunoreactivity (NTLI) concentration and to characterize NT receptor in the rat brain. The effect of various drugs affecting the dopaminergic system in the brain were also investigated to clarify the relationship between NT and dopamine. NTLI was widely distributed in various regions of the rat brain: the highest level was 53.1 +/- 14.1 ng/g wet wt in the hypothalamus, 11.9 +/- 6.0 ng/g wet wt in the thalamus, 6.9 +/- 0.7 ng/g wet wt in the cerebral cortex and 0.7 +/- 0.2 ng/g wet wt in the cerebellar cortex. The Bmax of NT receptor was 20.2 +/- 4.6 fmol/mg protein in the cerebral cortex, 16.0 +/- 4.9 fmol/mg protein in the hypothalamus, 13.3 +/- 3.7 fmol/mg protein in the thalamus and no detectable level in the cerebellar cortex. The Kd of NT receptor was 2.7 +/- 0.7 nM in the cerebral cortex, 1.9 +/- 0.8 nM in the hypothalamus, 1.1 +/- 0.2 nM in the thalamus. Intraperitoneal(ip) bolus administration of L-DOPA caused the reduction in NTLI concentration from 9.2 +/- 1.0 ng/g wet wt to 5.3 +/- 2.1 ng/g wet wt and Bmax of NT receptor from 19.7 +/- 3.1 fmol/mg protein to 13.9 +/- 2.6 fmol/mg protein with no change in Kd. Intracerebroventricular administration of dopamine also induced a significant reduction of NTLI concentration and Bmax of NT receptor with no change in Kd. Inversely, ip bolus administration of alpha-methyldopa evoked an increase in NTLI concentration from 6.9 +/- 0.9 ng/g wet wt to 12.0 +/- 3.0 ng/g wet wt and that in Bmax of NT receptor from 15.9 +/- 2.9 fmol/mg protein to 24.2 +/- 4.6 fmol/mg protein. Serial administration of haloperidol evoked an increase in Bmax of NT receptor from 16.9 +/- 1.5 fmol/mg protein to 20.5 +/- 1.9 fmol/mg protein with no changes in Kd and NTLI concentration. These results suggest that dopamine may modulate the action of NT in the rat cerebral cortex.

Animals↗
PubMed1988

Changes in polyadenylation of lactate dehydrogenase-X mRNA during spermatogenesis in mice.

The expression of the mRNA for mouse testicular lactate dehydrogenase (LDH-X) was examined by RNA:cDNA hybridization in situ in the testis and by Northern analyses of meiotic and postmeiotic spermatogenic cell populations. Silver grains accumulated in cells inside the second layer from the periphery of the seminiferous tubule, confirming previous findings that LDH-X mRNA first appears in the spermatocyte and continues to accumulate until the late spermatid stage. Northern analyses showed that meiotic and postmeiotic cells contained 1.2 and 1.3 kb classes of hybridizing mRNA, respectively. RNase H digestion of oligo (dT)-hybridized RNA and poly(U)-Sepharose column chromatography with differential elution by formamide revealed that the difference in size of the two classes of mRNAs was due to the poly(A) tail length of the LDH-X mRNA. When the distribution of the LDH-X mRNA was examined across polysome gradients, both mRNAs were partially associated with polysomes. These results suggest that the changes in the polyadenylation of LDH-X mRNA were associated with the meiotic division during spermatogenesis in the mouse. They raise the possibility that the stable accumulation of the LDH-X mRNAs in the postmeiotic cells is enhanced by poly(A) tails of increased length.

Animals↗
PubMed1988

Non-specific suppressor cells in murine bone marrow chimeras: their possible role in GVHD-associated immunodeficiency.

Suppressor cells against several mitogen-induced responses were detected in the spleen of murine bone marrow chimeras, regardless of intravenous (i.v.) or intrasplenic (i.s.) bone marrow transplantation (BMT). According to the time-course of the suppressor activity against Con A, PHA, and PWM, they were readily detected at 11-21 days after BMT and thereafter, either gradually decreased or remained at a plateau level. In contrast, the suppressor activity against the LPS-stimulated response increased at 39-52 days as compared to 24-34 days after BMT. Characterization studies of suppressor cells early (11-21 days) after BMT revealed that those in the i.v. and i.s. chimeras were composed of host-derived plastic dish adherent and/or anti-Thy 1.2 antibody-insensitive spleen cells in general. On the contrary, those in the i.v. and i.s. chimeras that possessed severer GVHD were mainly composed of host-derived plastic dish non-adherent spleen cells. Since the suppressor activity was higher in chimeras with severe graft-versus-host disease (GVHD) than in conventional chimeras, suppressor cells against the mitogen-induced responses may be related to the immunodeficiency associated with GVHD. Particularly, plastic dish non-adherent suppressor cells may closely relate to GVHD-associated immunodeficiency as compared with plastic dish adherent suppressor cells.

Animals↗
PubMed1988

Suppressor activity in the supernatant of T cell clones derived from chimeric mouse spleen cells.

The supernatant from cultures of T cell clones derived from (BALB/c----C3H/He) chimeras suppresses BALB/c anti-C3H/He or BALB/c anti-C57BL/6 MLRs. When we studied the alloantigen specificity of the suppressor activity in culture supernatant, we observed three types of the suppressor activity (i.e., the suppressor activity against BALB/c anti-C3H/He MLR, against BALB/c anti-C57BL/6 MLR, and against both MLRs) on day 3 after stimulation of the T cell clones with 20% crude IL2 and feeder cells. Since the alloantigen specificity fluctuated somewhat with time, we considered that a time-course study was needed to determine it correctly. We thought it unlikely that any IFN-gamma or PGE2 in the culture supernatant of the T cell clones would have mediated the suppression. Our results suggest that alloantigen specific and non-specific suppressor T cells exist in bone marrow chimeras. The former appears to play an important role in inducing and maintaining transplantation tolerance, while the latter seems to have a rather harmful effect upon chimeras.

Animals↗