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Biomedical subjects

H Frey

Publications and source records attributed to H Frey.

At least 145 records · Page 8Linked to original sources
PubMed1976

Serum concentration of carbamazepine: comparison of Herrmann's spectrophotometric method and a new GLC method for the determination of carbamazepine.

A specific direct gas chromatographic method to determine carbamazepine and, semiquantitatively, 10,11-epoxy carbamazepine in serum is described. The average recovery of carbamazepine is 98%, and the error on duplicate determination is +/- 4%. The method is compared with Herrmann's classic spectrophotometric method. In material of 103 patients the mean serum concentration of carbamazepine was 25.5 +/- 12.8 mumoles/1 with GLC and 23.0 +/- 12.6 mumoles/1 with spectrophotometry. The difference was highly significant. The blood sample volume is one-tenth of that needed in spectrophotometry.

Carbamazepine↗
PubMed1976

Pathogenesis of multiple sclerosis. Neurochemical and morphological studies on MS biopsies and autopsies.

In the present study, six multiple sclerosis (MS) brain white matter biopsies were analyzed both morphologically and chemically. The purpose of this study was to test the reliability of the earlier postmortem observations. Special attention was given to proteins which were analyzed in polyacrylamide disc gel electrophoresis and to acid proteinase. The results show that myelin basic protein (BP) was present in all biopsies, although advanced demyelination was seen in one case as based on morphology. This finding is controversial to earlier observations. In addition to this, some acid proteins were decreased or lost in two cases. The activity of acid proteinases was increased from two- to fivefold in four of six cases. This increase of the activity was seen also in the normal-appearing MS autopsy white matter which served as control. The source of this activity is discussed. On the basis of the present results it is likely that the role of the BP in the breakdown of myelin in MS and the role of acid proteinases has to be studied in more detail.

2',3'-Cyclic-Nucleotide Phosphodiesterases↗
PubMed1976

Biological rhythm of cell-mediated immunity in man.

The biological rhythm of the spontaneous lymphocyte transformation and the lymphocyte response to phytohaemagglutinin (PHA), concanavalin A (Con A) and purified protein derivative of tuberculin (PPD) was studied in six healthy male volunteers. The count of peripheral blood lymphocytes was increasing from 08.00 until 02.00 hr. The level of plasma cortisol was highest at 08.00 hr and lowest at 20.00 hr. The spontaneous lymphocyte transformation showed a biphasic cycle during the day; the peak values were observed at 08.00 and 20.00 hr and a significant depression was found at 14.00 hr. The response of peripheral blood lymphocytes to PHA and Con A varied inversely with the level of plasma cortisol. The PPD response was lowest at 08.00 hr, but the highest value was observed at 02.00 hr. No significant day to day variation was found in any of the parameters studied.

Adult↗
PubMed1976

[Demonstration of antigens of bone and articular connective tissue in the blood of rats with adjuvant arthritis].

The results reported in this paper clearly indicate the release of osteal antigens of articular connective tissue in response to inflammatory alterations and injuries to the region immediately adjacent to an articulation. Further studies will have to be performed in an effort to find out whether these antigens are of pathological importance and if there is a relationship between the liberation of antigens and the intensity of inflammatory alterations or if the different and often difficult-to-interpret results of the determination of antibodies in the sera of patients with rheumatoid arthritis can be more easily explained by a simultaneous antigen analysis of the corresponding sera.

Animals↗
PubMed1976

Intestinal absorption of calcium from three commerical calcium preparations in man.

Intestinal 47Ca absorption was determined from blood and stool radioactivity after oral administration of 150 ml saline containing the isotope (as 47CaCl2) and 500 mg of elementary calcium in the form of one of three commercial calcium preparations from "NAF" or "Collett" (containing calcium carbonate) or from "Sandoz" (containing mainly calcium lactogluconate). 51CrEDTA was used as unabsorbable marker to allow corrections for incomplete faecal collection. The calcium carbonate preparations were only partially dissolved when administered, but the calcium absorption from these preparations was found to be no less than that from the completely soluble calcium "Sandoz" preparation. The calculated minimal absorption of calcium was, on an average, about 100 mg from all three calcium preparations in subjects without known intestinal calcium absorption defects.

Administration, Oral↗
PubMed1975

Thyroid function during erythrosine ingestion in doses encountered in therapy with conventional antibiotics.

Six euthyroid patients without clinical or biochemical evidence of any renal or thyroid disorder received a diet of fixed composition. They were given no drugs. Urine was collected quantitatively throughout the whole investigation for determination of iodine and creatinine. After 5 days on diet they received 1.91 mumol of erythrosine, corresponding to approximately 7.88 mumol of iodine daily for 10 days. Thyroidal radioiodine studies were performed, levels of plasma inorganic iodine were calculated, and thyroxine, protein-bound iodine, and total iodine in serum were determined before and during erythrosine ingestion. A statistically significant increase in plasma inorganic iodine or in urinary iodine excretion was not found. The other test results of thyroid function remained virtually unchanged. Based on clinical aspects of thyroidal iodine metabolism we conclude that at most 7.8 per cent of the iodine content of the ingested erythrosine could have supplemented the iodine space.

Adult↗
PubMed1975

The acute effect of sodium cellulose phosphate on intestinal absorption and urinary excretion of calcium in man.

Intestinal 47Ca absorption has been determined from blood and stool radioactivity after oral administration of the isotope in nine patients before and during cellulose phosphate treatment. Oral administration of 5 g cellulose phosphate concomitant with 47Ca in 100 mg "carrier" calcium as CaCl2 decreased 47Ca absorption by 80 per cent. Cellulose phosphate, 5 g three times daily, decreased urinary excretion of non-radioactive calcium by 47 percent. Urinary magnesium excretion decreased by 47 percent whereas urinary phosphorus excretion increased by 67%. Calcium and magnesium excretion in urine decrease because cellulose phosphate binds divalent cations within the GI tract. The increased phosphorus excretion is probably due to partial hydrolysis of the substance in the gut.

Calcium↗