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Biomedical subjects

H Fey

Publications and source records attributed to H Fey.

At least 37 records · Page 2Linked to original sources
PubMed1981

[Tetanus antibody screening in blood donors].

Two ELISA test methods for detection of tetanus antibodies are presented which are suitable for routine screening. Administration of the tests to blood donors shows good average immunization of the Swiss population against tetanus. Due to compulsory immunization during military service, men exhibit markedly higher antibody levels against tetanus than women. Some of the tested blood donors have rather high tetanus antibody titers, and it would be worth collecting plasma from these donors by plasmapheresis for production of a high-titred antitetanus hyperimmunoglobulin.

Adult↗
PubMed1981

Measurement of staphylococcal protein A and detection of protein A-carrying staphylococcus strains by a competitive ELISA method.

A competitive ELISA for the estimation of staphylococcal protein A is described. Tetanus toxoid is insolubilized on polystyrene and incubated with human antitoxin, which renders the Fc-piece of this antibody freely accessible to protein A. The binding of the latter is demonstrated by its competition with protein A-phosphatase conjugate. The method has been shown to be sensitive and reproducible. It has been used for the detection of protein A in culture supernatants and on living pathogenic staphylococci. The test could therefore be of diagnostic value. Protein A is also present in extracts of food contaminated with enterotoxic staphylococci. It can be eliminated by a simple absorption with insoluble porcine IgG.

Animals↗
PubMed1981

Use of a computer to evaluate sigmoidal curves in serology by a new procedure.

Serological standard curves are mostly sigmoidal in shape. Their transformation into straight lines by linear regression can be the source of serious error. Log/log or logit/log handling of the values can straighten the curve but only if their distribution is normal. A new way of calculating concentrations of antibody or antigen which leaves the standard curve unmanipulated is described. Computer programs for TI 59 (Texas Instruments) and--in BASIC--for a personal computer have been written and greatly facilitate routine work.

Computers↗
PubMed1981

Improvement of the polyvalent Salmonella phage's O-1 diagnostic value by addition of a phage specific for the O groups E1-E4.

The usefulness of the polyvalent Salmonella phage O-1 as a first step diagnostic tool is again emphasized. 96.1% of all Salmonella strains are lysed. Its disadvantage of not lysing some 10-30% of strains belonging to the O groups E1-E4 has been eliminated by creating a mixture of the phage O-1 with a phage G47, (obtained from Gershman) which is highly active on OE strains. Thus the mixture exhibits a high OE-specificity without impairment of the O-1 polyvalency.

Bacteriolysis↗
PubMed1980

Enzyme linked immunoassay and fluorescent antibody techniques in the diagnosis of viral diseases using staphylococcal protein-A instead of anti-gamma-globulins.

Staphylococcal protein-A (SpA) is known to interact with the crystallizable fragment (Fc) of IgG molecules from several species. In the present study, SpA coupled to either fluorescein isothiocyanate (FITC) or peroxidase was used in place of antisera to IgG for the fluorescent antibody (FA) techniques and the enzyme linked immunoassay (ELISA). The SpA conjugates produced low background staining when applied in these techniques, and provide a rapid, highly specific and sensitive means for the identification of viral isolates and the detection of serum antibodies. Moreover, SpA is a single reagent that replaces various preparations of anti-gamma globulin against many species. SpA-FITC conjugate was successfully applied for the identification of pseudorabies virus, hog cholera virus, swine vesicular disease virus, transmissible gastroenteritis virus, porcine parvovirus and porcine enteroviruses. Antibody titers against the mentioned viruses could be determined semi-quantitatively in the indirect FA test with SpA-FITC. In our laboratory the ELISA became a routinely practicable serological test for the detection of antibodies only after we introduced SpA-peroxidase as a marker for the IgG.

Animals↗
PubMed1980

Visualization of immunosorption by scanning electron microscopy.

Immunosorption has become a very important biochemical and serological tool and it is the purpose of this paper to visualize this process qualitatively using the scanning electron microscope. Different carriers (i.e. CNBr activated cellulose and Sepharose, glutaraldehyde treated acrylamide-agarose beads Magnogel, and polystyrene cover slips) were coated with different antibodies and incubated with their homologous antigens such as pneumococci, ferritin, polymeric Salmonella flagellin and mechanically detached flagella, as well as tetanus toxoid, E. coli bacteriophage T4 and Rota virus particles. As negative controls the sorbents were incubated with heterologous antigens.

Animals↗
PubMed1979

Antibody coated bacteria in urine sediment from cattle infected with Corynebacterium renale.

Using the fluorescent antibody test, the presence of antibody-coated bacteria in 10 out of 17 urine sediment samples from cattle infected with Corynebacterium renale is described. These antibodies were mainly of the immunoglobulin class IgG, and to a lesser extent IgA. This finding is characteristic for infections of the upper urinary tract (pyelonephritis). In seven samples no antibody coating of the bacterial surface was detected. In these cases an infection of the lower urinary tract (cystitis) is suggested.

Animals↗
PubMed1979

A novel method for the production of Salmonella flagellar antigen. II. Further purification for the preparation of H antisera.

A method for a simple preparation of Salmonella flagellar antigen is described. The antigen is sufficiently pure to elicit high titered H antibodies of 12,800-51,200 and O titers of less than 50. Highly motile Salmonella test strains are grown on 0.8% swarm agar and harvested with 0.05 n HCl which solubilizes the flagella. The suspension with a pH of 1.5 is kept at 4 degrees C. over night and then centrifuged at 49,000 g for 60 min. The supernatant is neutralized and precipitated with ammonium sulfate at 2/3 saturation. The resulting polymeric flagellin is submitted to a zone electrophoresis on Pevikon. Strips are cut from the "cake" and eluted. The H antigen is found on the anodic side, the O antigen remains near the trough or migrates slightly cathodically. Form 20 plates enough flagellin is collected fro the immunization of 50-100 rabbits. The Latex test proved to be especially suited for the checking of the H antigen.

Animals↗
PubMed1978

Simple assay for staphylococcal enterotoxins A, B, and C: modification of enzyme-linked immunosorbent assay.

The enzyme-linked immunosorbent assay (ELISA) introduced for the detection of staphylococcal enterotoxins by Saunders et al., Simon and Terplan, and ourselves has proved to be a simple, reliable, and sensitive test. A new modification is described that uses polystyrene balls (diameter, 6 mm) coated individually with antibody against one of the toxins A, B, or C. In a single tube, 20 ml of the food extract was incubated with the three balls differently stained, which were then each tested for the uptake of enterotoxin by a competitive ELISA. A concentration of 0.1 ng or less of enterotoxin per ml can be measured, making tedious concentration procedures of the extracts superfluous. Culture supernatants and extracts from foods artificially or naturally contaminated with toxin were successfully examined. Cross-reactions did not occur, and nonspecific interfering substances did not create serious problems.

Enterotoxins↗