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Biomedical subjects

H F Otto

Publications and source records attributed to H F Otto.

At least 127 records · Page 7Linked to original sources
PubMed1987

[Abdominal pseudotumor as the clinical manifestation of Whipple's disease. T-cell index as an indicator of disease activity and a parameter of the duration of therapy?].

A large nonresectable upper-abdominal tumor in a 61-year-old woman was found to have been caused by inflammatory infiltration and mechanical obstruction of the mesenteric lymph nodes resulting in obstruction to lymphatic flow. Biopsy at the root of the mesentery and of abdominal lymph nodes raised the suspicion of Whipple's disease, a diagnosis confirmed by a small-intestine biopsy. Long-term antibiotic treatment was successful. Quantitative changes in the composition of the T-cell subpopulations at the time of the diagnosis reverted to normal in the course of treatment.

Abdominal Neoplasms↗
PubMed1987

Thymic hyperplasia. I. True thymic hyperplasia. Review of the literature.

The condition of true thymic hyperplasia is defined as an increase of both size and weight of the gland while it maintains normal microscopic architecture. It occurs in three different clinicopathologic forms: True thymic hyperplasia without any other disease is extremely rare and only seven well documented examples could be found in a review of the literature. Enlargement of the thymus gland is reported as a form of rebound phenomenon in a number of conditions like recovery from severe stress situations, after administration of steroids, and after treatment of malignant tumors. Finally, thymic hyperplasia has been described in association with endocrine abnormalities, sarcoidosis, and Beckwith-Wiedeman syndrome.

Child↗
PubMed1987

Thymic hyperplasia. II. Lymphofollicular hyperplasia of the thymus. An immunohistologic study.

The so-called lymphofollicular hyperplasia, which is caused by the occurrence of hyperplastic lymph follicles within the organ, is constantly associated with autoimmune diseases (e.g., myasthenia gravis) and in rare instances with malignant tumors. The architecture of lymphofollicular hyperplasia was studied immunohistochemically using antibodies against epithelial, vascular, lymphocytic, and histiocytic antigens. There is evidence, that the configuration, microtopography, cellular composition, and immunohistological findings of the lymph follicles with germinal centers in the myasthenic thymus are essentially the same as in those occurring in lymph nodes and in other lymphatic tissue. Furthermore it could be shown that the follicles originate in the interlobular septal space and displace the thymic parenchyma by extension.

Adolescent↗
PubMed1987

Cytokeratin expression in normal salivary glands and in cystadenolymphomas demonstrated by monoclonal antibodies against selective cytokeratin polypeptides.

The distribution of selective cytokeratin polypeptides, vimentin, and glial fibrillary protein (GFP) in 5 human cystadenolymphomas of the parotid gland was compared with normal human parotid (n = 5) and submandibular (n = 4) glands using a panel of monoclonal antibodies against diverse and selective cytokeratin polypeptides, vimentin and glial fibrillary protein (GFP). A biotinstreptavidin method was used on cryostat sections. The immunocytochemical finding of identical cytokeratin polypeptides Nos. 7, 8, 18 and 19 and basal cells selectively labeled by the monoclonal antibody KS 8.58, in both the epithelial part of the cystadenolymphomas and in the duct epithelium of the parotid gland, confirms the hypothesis that the epithelial compartment of cystadenolymphomas is derived from the duct system. The triple expression of cytokeratin, vimentin and GFP in myoepithelial cells of the parotid gland is discussed.

Adenolymphoma↗
PubMed1987

Sequential induction of MHC antigens on autochthonous cells of ileum affected by Crohn's disease.

Changes were examined in the expression of Class I and II major histocompatibility complex (MHC) antigens by autochthonous cells of the terminal ileum affected by Crohn's disease. The study was based on the analysis of transmural specimens from terminal ileum segments obtained in the course of ileocolectomy for colon cancer and Crohn's disease. Serial sections were immunostained using monoclonal antibodies directed against monomorphic determinants of HLA-A,B,C, DR, DP, DQ, and the invariant chain (Ii) associated with Class II molecules. Compared with the normal state, the only change in Class I antigen expression occurring in Crohn's disease was the induction of HLA-A,B,C antigens in lymphatic endothelium. Changes in Class II antigen expression were more substantial. Enhancement of HLA-DR expression was found in enterocytes; DR induction was observed in glial cells of the visceral nervous plexus and in venular and venous endothelium. HLA-DP and DQ antigens were induced in enterocytes, glial cells, and capillary and venular endothelium, although this induction was restricted to areas of moderate or high inflammatory activity. The tissue distribution of Ii closely resembled that of HLA-DR, although this association was not strict: on the one hand, arterial endothelium contained low amounts of Ii in the absence of DR antigens; on the other hand, glial cells expressed Class II molecules in the absence of Ii. The extent of local enhancement/induction of MHC antigens was positively correlated with the local density of the cellular infiltrate. These data suggest that altered MHC antigen expression by autochthonous structures might be mediated by factors released from the lymphohistiocytic infiltrate, which is itself attracted by an unknown signal. In conjunction with an unknown antigen, the enhanced expression of Class II antigens might trigger an autoaggressive immune response.

Adolescent↗
PubMed1986

Primary mediastinal clear cell lymphoma of B-cell type.

This is a report on 8 mediastinal tumours that occurred in young adults (19-43 years, mean: 29.4); predominantly in females (6/8). Initial symptoms consisted of thoracic pain and venectasia and in only one case in B symptoms. After surgical tumour reduction, radiation and/or chemotherapy, local recurrence was observed in each case under clinical care; abdominal spread is presently suspected in 3 patients; 3 died 11, 13 and 22 months after diagnosis. None developed leukaemia. The tumours are B-cell neoplasms with a characteristic immunophenotype: leucocyte common antigen+, common acute lymphoblastic leukaemia antigen-, B 1-antigen+, surface and cytoplasmic immunoglobulin-. Flow cytometry revealed DNA-diploidy in 7 cases and a moderately (3.2-3.8%) to extremely high (8.0-20.6%) S-phase component. The proliferation associated antigen Ki67 was detectable in 10-60% of the tumour cell nuclei, thus stressing the considerable or rapid growth. Histopathology is characterized by a diffuse growth pattern and a clearness and abundance of cytoplasm of the pleomorphic tumour cells, which vary in size and nuclear morphology from patient to patient. Apoptoses are more numerous than mitoses. Fibrosis and focal necrosis are common, sclerosis is present in 3 cases. We suggest that primary mediastinal lymphoma of B cell type is a novel B-lymphoma variant.

Adult↗
PubMed1986

The primary mediastinal clear cell lymphoma of B-cell type has variable defects in MHC antigen expression.

Eight cases of the recently reported 'primary mediastinal clear cell lymphoma of B-cell type' (Möller et al., 1986) were examined immunohistologically for the expression of cytoplasmic and/or surface antigens of MHC class I and II with mAbs directed against framework determinants of HLA-A,B,C (W6/32; B9.12.1), HLA-DP,DR,DQ (2.06), -DQ (Leu 10; Tü22), -DR (Tü34) gene products, and with mAbs specific for beta 2-microglobulin (BBM-1) and the HLA-D associated invariant chain (Vic-Y1). Besides the reported Ig-deficiency, the neoplastic B-cells of 7/8 tumours have variable defects in MHC antigen expression. Three lack both class I and class II antigens, one tumour lacks class I antigens but expresses HLA-DQ and -DR on the majority of neoplastic cells, three others contain varying proportions of MHC-antigen deficient tumour cells. The expression of Ii is closely correlated with HLA-D(R) expression and its antigenic sites are strictly located in the cytoplasm. Against the background of current knowledge, the variable and occasionally severe defects in MHC antigen expression within the herein presented series of B-cell lymphomas suggest that this unusual feature might be another characteristic of a novel lymphoma type.

Adult↗
PubMed1986

[Immunohistologic analysis of chronic obstructive sialadenitis. I. Changes in the expression of antigens of the major histocompatibility complex].

In the course of electrolyte sialadenitis progressing towards obstructive sialadenitis, changes in the epithelial expression of antigens of the major histocompatibility complex (MHC) occur even before morphological alterations and inflammatory cells can be detected. Class I antigens are enhanced and induced, and Class II antigens are induced. These changes begin focally. Only the expression of HLA-A,B,C and -DR in acinar epithelium is linked to the close vicinity of lymphohistiocytic infiltrates. In later stages of the disease the foci tend to fuse and antigenic changes are pronounced in areas of fibrosclerosis and parenchymal atrophy. The altered MHC antigen expression induced by chronical saliva congestion probably represents an important part of the postulated "immunoreaction in the duct epithelium" supposedly giving rise to this type of immunosialadenitis.

Adult↗
PubMed1986

[Well-differentiated liposarcoma of the submandibular region. Case report and literature review].

Liposarcoma of the head and neck are seen only occasionally. In particular manifestations of this tumour in the submandibular region or the floor of the mouth are very rare. The pathohistological features and the clinical picture of these tumours are described by means of a case report and a review of the literature. The close correlation between histological differentiation and prognosis of liposarcoma is underlined. The recommended possibilities of treating these rare soft tissue tumours are discussed.

Adipose Tissue↗
PubMed1985

Skeletal muscle antibodies in patients with a thymic tumour but without myasthenia gravis.

Sera from 9 patients with a primary thymic tumour but without myasthenia gravis (MG) at the time of thymectomy were examined for skeletal muscle antibodies. Antibodies to a citric acid extract (CA), associated with the presence of a thymic lymphoepithelioma in MG patients, were detected in 3 sera. AChR antibodies were detected in 5 sera, the concentration markedly elevated in 1 of them. Sarcolemmal and cross-striational antibodies were detected in 2 and 3 sera, respectively. Four of the 5 patients with thymic tumours other than a lymphoepithelioma had AChR antibodies, and 3 of them also had CA antibodies.

Adult↗
PubMed1985

[Kaposi sarcoma in acquired immune deficiency syndrome (AIDS). II: Light and electron microscopic and immunohistochemical peculiarities including the cytoskeleton].

AIDS-related lymphoadenopathy is characterised by follicular hyperplasia with irregularities in the dendritic network, a loss of the follicular mantle zone and an enhanced influx of T-suppressor cells into the germinal centres. The histological features of AIDS-associated epidemic Kaposi's sarcoma are spindle-like tumour cells and a source vascularisation, which is of clinical relevance for the biopsy. Since immunohistochemical markers specific for blood vessel endothelium can not be detected within the tumour cells an origin from this cell type gets unlikely. An origin from lymphatic endothelium however can still be discussed.

Acquired Immunodeficiency Syndrome↗
PubMed1985

Thymoma. A clinicopathologic study of 98 cases with special reference to three unusual cases.

98 thymomas were assessed in respect to clinical manifestations, gross and histologic pathological findings and clinicopathologic correlations. 34% of patients were asymptomatic and thymoma was detected fortuitously. The most common presenting symptoms were related to myasthenia gravis, symptoms due to pressure on mediastinal structures were next in frequency. The symptom-diagnosis interval ranged from 0 to 120 months with a median of 4,5 months and was longer in invasive thymomas (median 6 months) than in noninvasive thymomas (median 2 months). 52% of thymomas were encapsulated and showed no cytologic atypia and were therefore classified as benign encapsulated thymomas. 26% showed gross invasion of peripheral structures and 3% were thymic carcinomas on histologic grounds. Histologically 55% of thymomas were epitheloid cell type, 17% spindle cell type and 20% mixed type thymomas. Epidermoid type thymoma occurred in 3% of the cases. 3 cases showed some unusual morphologic feature: one was localized intrapulmonal, another had an outspread like a mesothelioma, and the third was a basaloid carcinoma with unusual goblet cell metaplasia. In the three cases immunohistological methods were used as a diagnostic tool. The lectins UEA-I, PNA and HPA and an anti-keratin allowed the diagnosis of epithelioma (in 2 cases) and showed some more cellular and structural differentiations (in 1 case).

Adolescent↗