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Biomedical subjects

H Eder

Publications and source records attributed to H Eder.

71 records · Page 4Linked to original sources

[Circumfusion chamber for microscopic examination of living single cells and other particles].

A new circumfusion chamber is described for microscopic or micrometric investigations on living single cells and similar particles. The chamber allows to collect rare single cells. Cells are fixed within the optical field or measuring field in spite of relative high velocity of the medium. Rapid changes of the cells milieu are possible, reactions of the cell are individually visible or measureable. The chamber is temperated and allows application of different special microscopic techniques.

Blood Cells↗

[Fluorescence microscopic demonstration of eosinophile leucocytes with different fluorochromes (author's transl)].

A screening program involving blood from men and horse resulted in about 80 fluorochromes admitting microscopic differentiation of eosinophile leucocytes (properly their granules) in blood smears. Fluorescence distinguishes either selective, i. e. only eosinophile cells show fluorescence, or specific, that means, eosinophilic granules exhibit fluorescence differing from non-eosinophilic cells by colour and/or intensity.

Animals↗

[Serum potassium and urine potassium concentrations following single and repeated potassium load].

Levels of potassium concentration have been determined after single and repeated oral application of potassium. The maximum level of serumpotassium concentration was found about 2 hours after intake of 120 mval potassium. The concentration of potassium did not return to basic values within 24 hours. The concentration of potassium in urine increased during the first 4 hours. 6 to 12 hours later the maximum level is achieved. This leads to the conclusion that enterally applicated potassium is shifted into the intracellular space and is later released into the extracellular space. Repeated application of potassium in diminished dosages leads to reduced increased of serum and urin potassium concentration. The increase in concentration remains relatively constant during the whole period of application. For longterm therapy with oral potassium an application of a minimum dosage of 40 mval potassium in an 8 to 12 hours rhythm is reasonable.

Extracellular Space↗

Importance of fat conservation in lower blepharoplasty.

The authors describes her experience in over 200 cases operated on from 1993 to 1996 with the lower blepharoplasty with arcus marginalis release and fat conservation as described by Hamra [Plast Reconstr Surg 96:354, 1995; Clin plast Surg 23:17, 1996]. The overall satisfaction with the somewhat modified technique led the author to abandon completely the conventional type of fat resection. The technique has been used in different clinical situations. The anatomical basis, the technique, and the complications are discussed.

Adipose Tissue↗

On the relationship between measles virus and Alzheimer neurofibrillary tangles in subacute sclerosing panencephalitis.

We have studied the relationship between measles virus and the accumulation of abnormally phosphorylated tau (PHF-tau) in nine cases of subacute sclerosing panencephalitis (SSPE). By assessing the presence of viral intranuclear inclusions and neurofibrillary tangles (NFT) in each case, we found no correlation between presence and amount of measles virus and the numbers of neurons containing PHF-tau. Immunohistochemical double labeling in a case with long duration of disease and severe histopathologic change revealed no strict colocalization of measles virus antigen and PHF-tau throughout different brain regions. In areas containing both antigens, most neurons carrying measles virus did not have a tangle and vice versa, eventhough some colocalization beyond that expected by chance was observed in specific cortical areas. These results indicate that, although secondary to viral infection, NFT formation in SSPE is not restricted to cells carrying viral antigen. Conversely, measles virus infected cells do not necessarily accumulate PHF-tau. This lack of colocalization at the cellular level, throughout different brain areas and among different cases suggests that the formation of NFT in SSPE is not directly induced by the infectious agent. The formation of NFT in this disease appears to be elicited through a specific type of tissue damage and, thus, to be an epiphenomenon. This pathogenetic detail may be of interest for our understanding of the role of neurofibrillary degeneration in the pathogenesis of other more frequent neurodegenerative diseases with cytoskeletal pathology.

Adolescent↗

Breast reconstruction at the tumor center of the University of Brussels. A three year report.

In 80 cases, breast reconstruction was performed as a secondary procedure at least one year after mastectomy for cancer. The prerequisite was absence of local recurrence and distant metastasis, good general condition and careful information on the result to be expected. In the majority of the cases reconstruction was accomplished in two stages: volume replacement with a prosthetic implant was followed after 3 months, by nipple-areolar reconstruction-usually from the opposite side. Capsulotomy or replacement of the prosthesis in case of asymmetry could be performed at this time. If necessary, skin replacement was done as a first procedure by means of a thoraco-epigastric or a latissimus dorsi myocutaneous flap. Aesthetic results are improving with experience and the positive psychological response of the patients is an encouragement to proceed in helping also patients with a rather poor prognosis.

Adult↗

Reconstruction of the nipple-areolar complex after radical mastectomy.

Reconstruction of the nipple areolar complex should be done as a second stage procedure about three months after volume replacement. Symmetry in position, size, and color is of utmost importance for the overall aspect of a breast reconstruction. One piece full thickness grafts from the opposite areola and nipple give the best results. When the remaining areola is too small to provide a full thickness graft, shaving of its surface may be used but often leads to later depigmentation. In these cases inguinal or perineal full thickness skin grafts seem preferable. Projection of the nipple remains a difficult goal. Experience of the authors with 74 nipple areolar reconstructions is discussed.

Breast↗