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Biomedical subjects

H Eckert

Publications and source records attributed to H Eckert.

At least 37 records · Page 2Linked to original sources
PubMed1991

Proliferative capacity of mononuclear cells in the human lung.

In pulmonary sarcoidosis, a chronic granulomatous disorder with different stages of activity, the proliferative capacity of the alveolar mononuclear cells is unknown. To get a closer look at this proliferation we combined pulse labeling (by means of tritium thymidine incorporation and autoradiography) with an immunocytochemical staining assay. This assay revealed on one single slide simultaneously blue CD4+ lymphocytes, brown CD8+ lymphocytes and red macrophages. We were able to show that CD4+ as well as CD8+ lymphocytes were radiolabeled only in the active state of the disease. But macrophage proliferation occurred independently of the activity of the disease. In other words with this combination of techniques, it is possible to differentiate, on a single slide, three subsets of mononuclear cells, in combination with their proliferative behavior.

Autoradiography↗
PubMed1991

[Immunohistochemically detected activity of lysozyme in sarcoidosis granulomas--a parameter for the activity of the illness process?].

In 30 patients with sarcoidosis we estimated immunohistochemically the activity of lysozyme in correlation to the activity of ACE in serum and found a positive correlation between these two parameters. We conclude, that the lysozyme content of sarcoidotic granulomas, estimated immunohistochemically, may be a useful morphological parameter of the activity of the disease, applicable on formol fixed and paraffin embedded bioptical material.

Adult↗
PubMed1991

[Splenectomy in sarcoidosis].

It is reported about 6 patients suffering from sarcoidosis with splenomegaly. In these cases indication for splenectomy was given by symptoms of hypersplenism, abdominal pain and ineffective cortisone therapy. In general after operation activity of sarcoidosis is unchanged. Different markers of activity were used after a time interval of 2-11 years. In one case it has to be discussed an overwhelming postsplenectomy infection (OPSI).

Adult↗
PubMed1991

[Clinical and histomorphologic findings in Wegener's granulomatosis].

The Wegener's granulomatosis is a rare primary systemic vasculitis. It is diagnosed more frequently in the last years. Because of various manifestations (rhinitis, sinusitis, otitis, arthralgie, "red eye", neuritis, carditis) and different symptomatics the disease is misjudged over month and years. Clinical findings, problems of bronchopulmonary and histomorphological diagnostics and differential diagnosis are discussed. The prognosis is favourable in cases of early and timely diagnosis and therapy.

Adult↗
PubMed1991

[Bronchoalveolar lavage in systemic scleroderma and systemic lupus erythematosus--differential cell values and enzyme cytochemistry].

The aim of the study was to determine the bronchoalveolar lavage (BAL) cell differentiation and the activity of beta-glucuronidase and N-acetyl-beta-D-glucosaminidase in alveolar macrophages. In 12 patients with systemic sclerosis (SS), 4 with systemic lupus erythematosus and 4 healthy controls BAL was performed. The activity of beta-glucuronidase and N-acetyl-beta-D-glucosaminidase was measured semiquantitative by means of cytochemical methods. Lymphocytes and neutrophils in BAL cell differentiation are increased, also the activity of beta-glucuronidase. The activity of N-acetyl-beta-D-glucosaminidase is decreased in SS and SLE in comparison with controls. The activity of beta-glucuronidase seems to be a marker of activity of alveolar macrophages in SS and SLE.

Acetylglucosaminidase↗
PubMed1990

[The specificity of so-called "smoker cells" in lung tissue].

A simple test for demonstration of smoker cells was verified on bronchoalveolar lavage (BAL) and cytological smears of lung tissue. Alveolar macrophages exhibit in 81% (BAL) respectively 86% (surgical material) of the smokers with Fettrot 7B a red colouring of the cytoplasm. Only few non-smokers show a positive reaction for smoker cells. In ex-smokers this positive reaction disappears after 7 month. We conclude, that this method is very sensitive but not absolutely specific for detecting smokers with a simple cytological test.

Adult↗
PubMed1988

A pilot study of 131I monoclonal antibodies in the therapy of leptomeningeal tumors.

A pilot study was performed to investigate the toxicity and therapeutic effect of radiolabeled antibody administered intrathecally in patients with leptomeningeal tumors. Five patients who failed conventional therapy received between 11 mCi and 40 mCi of radiolabeled antibody. The choice of antibody varied depending on the immunophenotype of the tumor. Therapy was well tolerated generally, with minimal acute toxicity. Four of five patients achieved an objective response to treatment that has been sustained for a period varying from 7 months to 2 years. No clinical signs of chronic toxicity have been observed in patients 1 and 2 years after therapy.

Adult↗