Biomedical subjects

H Chmielewski

Publications and source records attributed to H Chmielewski.

80 records · Page 5Linked to original sources

PubMedDate not supplied

[Mitogenic activity in vitro of lysosomal proteins of peripheral blood granulocytes in patients with multiple sclerosis].

Incorporation of 3H thymidine was investigated in cultures of lymphocytes obtained from peripheral blood of 33 multiple sclerosis patients and 20 healthy controls in presence of PHA or lysosomal proteins from granulocytes. The number of T lymphocytes was determined also in the peripheral blood of these patients. A rise was observed in the mitogenic activity of proteins obtained from lysosomal granules of granulocytes of multiple sclerosis patients at the time of disease exacerbation with a fall of this activity at the time of remission. At the same time a fall of T-lymphocyte count was observed in the blood of multiple sclerosis patients. The correlations between 3H-thymidine incorporation into lymphocytes in vitro, and the mitogenic activity of proteins obtained from lysosomal granules of granulocytes and the count of T lymphocytes are discussed.

Acute Disease↗

PubMedDate not supplied

[Certain parameters of platelets oxygen metabolism in patients with multiple sclerosis].

The hypothesis of Mickel (1975) on multiple sclerosis suggested disturbances of lipid peroxidation in that disease. The purpose of this report was an analysis of oxygen metabolism in platelets in that disease, taking into account the peroxidation of lipids and antioxidant enzymatic protection against oxygen stress. In the reported study the concentration of malonyl dialdehyde (MDA) and the activity of glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD-1) were determined in multiple sclerosis patients. The studied group comprised 20 non-smoking patients (9 men and 11 women) aged 21-61 years during disease exacerbation. The criteria of disease diagnosis were those suggested by Poser et al (1983). The obtained results may suggest abnormal acceleration of lipid peroxidation in platelets in this disease.

3,4-Methylenedioxyamphetamine↗

PubMedDate not supplied

[Hormonal disturbances in patients with multiple sclerosis].

The results of certain tests for the relationship between the hormonal system and the immune system may shed some light on the aetiology of autoimmune diseases, including multiple sclerosis. In patients with that disease signs are found of various hormonal disturbances. It is supposed that they are caused mainly by hypothalamic lesions, but they may influence the course of the disease. The paper is trial of a broader presentation of these problems.

Autoimmune Diseases↗

PubMedDate not supplied

[The effect of neck rotation on auditory evoked brainstem potentials in patients with degenerative cervical spine changes].

Using the neck rotation test during recording of auditory brainstem evoked potentials the authors examined 60 subjects aged 16-60 years, including 20 healthy ones and 40 with cervical spondylarthrosis. The Neuromatic 2000 C Dantec device was used. Half the patients with cervical spine changes had also receptive hearing damage of low degree. The study demonstrated that neck rotation affected the latency time of the analysed waves I and II. The time was significantly prolonged in the patients with spondylarthrosis. The authors believe that simultaneous prolongation of the mean latency between waves I-III suggests worse compensation of circulation in patients, especially in the initial part of the auditory pathway. The authors think that the neck rotation test could be used in the study of brainstem auditory evoked potentials as a challenge test.

Acoustic Stimulation↗

PubMedDate not supplied

[Somatosensory evoked potentials in the assessment of preserved function of long spinal cord pathways versus decisions of operation].

In the light of several presented clinical cases the authors point out the usefulness of somatosensory evoked potentials for the objective determination of the continuity of long pathways in the spinal cord, which in correlation with the clinical findings and results of imaging methods helps in making the decision of surgical intervention. The non-invasiveness of this method makes possible repeated safe control examinations for assessment of the dynamic of lesions in the cord and objective assessment of the neurological condition after operations on the cord.

Adult↗

PubMedDate not supplied

Activity of adenosine deaminase in red blood cells of patients suffering from multiple sclerosis treated with adrenocorticotropic hormone.

Adenosine deaminase (ADA) activity was studied in red blood cells of patients suffering from multiple sclerosis treated with adrenocorticotropic hormone (ACTH). ADA activity in hemolysates was determined according to the method of Hopkinson and calculated as units per g of hemoglobin. Activity of adenosine deaminase in healthy subjects was 0.871 +/- 0.251 U/g Hb. In patients with multiple sclerosis, before treatment ADA activity was 0.765 +/- 0.131 U/g Hb and was about 15.2% lower than in the control group (p < 0.02). After treatment with ACTH, ADA activity increased to 1.005 +/- 0.211 U/g Hb (p < 0.001). We have suggested that increased activity of adenosine deaminase in red blood cells of patients suffering from multiple sclerosis after treatment with ACTH is caused by diminution of superoxide generation, and therefore its sparing effect on cell membrane and enzyme is connected with membranes.

Adenosine Deaminase↗

PubMedDate not supplied

Red blood cell superoxide dismutase and catalase activities in patients suffering from multiple sclerosis treated with adrenocorticotropic hormone.

The purpose of this study was to determine superoxide dismutase (SOD) and catalase (CAT) activities in erythrocytes of patients with multiple sclerosis treated with ACTH. SOD activity in hemolysates was determined according to the method of Misra and Fridovich and calculated as units per gram of hemoglobin (Hb). CAT activity in hemolysates was determined with Beers and Sizer's method and expressed in IU/g Hb. SOD activity in control group was (1.61 +/- 0.45) x 10(3) U/g Hb whereas, the activity of CAT amounted to (5.88 +/- 1.36) x 10(4) U/g Hb. Before the treatment, SOD activity was decreased by approximately 20% ((1.25 +/- 0.25) x 10(3) U/g Hb) while that of CAT-by about 7.7% ((5.43 +/- 0.68) x 10(4) U/g Hb) in comparison to the normal control. After treatment with ACTH, activity of both enzymes increased: SOD-by about 34.4% to (1.68 +/- 0.38) x 10(3) U/g Hb and CAT-by about 7% to (6.29 +/- 0.55) x 10(4) U/g Hb. Results of investigations showed that ACTH caused an increase in CAT and SOD activities in erythrocytes of patients after three-week treatment.

Adrenocorticotropic Hormone↗

PubMedDate not supplied

[Profiles of daily secretion of ACTH, beta-endorphins and cortisol in patients after minor craniocerebral trauma].

In 12 men aged 20-25 men who had minor craniocerebral trauma the profiles of daily secretion of ACTH, beta-endorphin and cortisol were studied. The studies were carried out on the second day after the trauma. Plasma concentrations of the hormones were determined on 8.00, 12.00, 17.00 and 22.00 hours. Statistically significantly increased, in relation to healthy persons, (p < 0.01) values of plasma hormone concentration were found at all time points. In 50% of the patients (six cases) a disturbance was found of the profile of daily secretion of ACTH and beta-endorphin. The disturbance of the profile of cortisol secretion was found only in one patient.

Adrenocorticotropic Hormone↗