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Biomedical subjects

H Busch

Publications and source records attributed to H Busch.

At least 343 records · Page 19Linked to original sources
PubMed1976

Sequence homologies in mammalian 5.8S ribosomal RNA.

Oligonucleotide products of complete pancreatic or T1 RNase digestion or partial T1 RNase digestion of HeLa cell (human) and MPC-11 cell (mouse) 5.8S rRNA are identical with those obtained from Novikoff hepatoma (rat) 5.8S rRNA except for minor differences at the termini. pCp is the only major 5' terminus of both human and mouse RNAs; both pGp and pCp 5' termini were found in rat 5.8S RNA. Furthermore, HeLa cells contain C-U-U at the 3' end rather than the C-U terminus of mouse and rat. The results indicate that the nucleotide sequence has been highly conserved during the evolution of mammals and suggest that, as reported for 5S rRNA, this sequence is essentially constant throughout the Mammalia.

Base Sequence↗
PubMed1976

Phosphorylation and DNA binding of nuclear rat liver proteins soluble at low ionic strength.

Proteins were extracted from isolated rat liver nuclei with 0.15 M NaCl and 0.35 M NaCl at pH 8.0. The number of phosphoproteins in these extracts was determined by labeling with 32P and autoradiography after two-dimensional gel electrophoresis. Two proteins, B22p and B24p, contained small amounts of 32P and sedimented with the 30S nuclear informofer particle. With the exception of two phosphoproteins, CB and CN', all of the phosphoproteins found in the 0.35 M NaCl extract. Approximately 20% of the 0.15 M NaCl soluble proteins bound to rat liver DNA in 0.05 M KCl-0.05 M Tris-HCl (pH 8). Of these proteins, 1-2% bound to DNA in 0.15 M KCl and were eluted with 2 M KCl. This DNA bound fraction which contained both phosphorylated and nonphosphorylated proteins was similar in both the 0.15 and 0.35 M NaCl extracts. However, two major proteins (C13 and C14) and three minor proteins (C15, C25, Cg') were present only in the 0.15 M NaCl extract. The results of the present study show that there are marked similarities in the two-dimensional gel electrophoretic, phosphorylation, and DNA binding properties of rat liver nuclear proteins soluble in either 0.15 or 0.35 M NaCl.

Animals↗
PubMed1976

[New diagnostic strategies in psychiatry by means of video-technique. I. The use of time-blind video analysis for the evaluation of antidepressant drug trials (author's transl)].

The use of audio-video methods allowed the decrease of subjective factors influencing ratings of psychopathological phenomina. TV-stored interviews, recorded at separate days, may be compared almost simultaneously. In this study a rating of severity of depression was made of patients under antidepressive medication on days 0, 10, 20. (Mianserin was compared with Amitriptyline in a double-blind-trial). This method rendered it possible to detect significant differences between therapeutic profiles over time. The same result was reached independently by both investigators. The judgement of depression was based on a global impression and not on the evaluation of isolated psychopathological symptoms. No absolute measurement of depression was possible. The described method of a time-blind-analysis of TV-stored interviews constitutes a considerable progress in the assessment of a drug's profile in time, particularly if rating-scales are used in the same manner.

Amitriptyline↗
PubMed1976

[New strategies in psychiatric diagnosis through the use of audio-visual technics. 2. Importance of verbal and non-verbal information for assessment of depressive patients].

Sections of 150 seconds duration were taken on a random basis out of TV stored interviews of day 0, 10 and 20 of a double-blind antidepressive drug trial. Not identical sections were selected for the isolated audio and video channel and for the combined presentation in such a way that for each of the 20 patients three times three parts were presented for evaluation. It was the two rater's task to rank each patient's tapes in terms of the degree of depression. They were blind regarding the day of recording the interview (day 0, 10 or 20). The best results were reached with the combined presentation of the audio-video-channel while each channel taken seperately rendered less diagnostic information. The raters evaluated the material in two different modifications: a) Video, audio and than audio-video-channel or b) audio, video and audio-video-channel. The first version (a) was evaluated in a better way than the second one with respect to the content of diagnostic information. Furthemore we found that the evaluation of the visual information became less safe if the audio presentation preceded as in version b.

Amitriptyline↗
PubMed1976

A fetal protein in chromatin of Novikoff hepatoma and Walker 256 carcinosarcoma tumors that is absent from normal and regenerating rat liver.

Antibodies to chromatin proteins of Novikoff hepatoma cells formed precipitin bands in the double-diffusion immunoprecipitation assay with chromatin proteins of Novikoff hepatoma, Walker 256 carcinosarcoma, and 18-day fetal rat liver. The antigen used for preparation of antiserum was the chromatin proteins initially extracted with 3 M NaCl-7 M urea and soluble after dialysis to 0.14 M NaCl-0.35 M urea. The chromatin proteins used for analytical studies were extracted with 0.6 M NaCl containing 0.01 M Tris-HCl (pH 8) and 100 muM phenylmethylsulfonyl fluoride. Corresponding chromatin proteins of normal and 18-hr regenerating rat liver, heart, and kidney did not form precipitin bands. The antigen was purified from the chrmatin of Novikoff hepatoma cells by exclusion chromatography on Sephadex G-150 and preparative nondenaturing polyacrylamide gel electrophoresis. Its migration on denaturing sodium dodecyl sulfate-polyacrylamide gels corresponded to a molecular weight of 26,000. Amino acid analysis showed that the ratio of acidic to basic amino acids was 1.4 to 1.0. Evidence for its homogeneity included its migration as a single protein spot on two-dimensional polyacrylamide gel electrophoresis and its single lysine amino-terminal amino acid. This protein is a glycoprotein, as shown by the presence of 15 moles of galactosamine per mole of antigen. These studies demonstrate the presence of a fetal glycoprotein in the chromatin of two tumors that may have an important role in determining their gene products.

Animals↗
PubMed1976

Evolutionary trends in 18S ribosomal RNA nucleotide sequences of rat, mouse, hamster and man.

The large T1 ribonuclease fragments of 18S ribosomal RNA from four mammalian species, rat, mouse, hamster and man, were compared by two-dimensional homochromatography fingerprinting. The nucleotide sequences of the large T1 ribonuclease fragments, polypyrimidines and polypurines which were different among the four mammalian species were determined and compared. The method used for determining nucleotide sequences utilizes 32p-labeling of oligonucleotides at their 5'-termini by polynucleotide kinase, partial digestion by ribonucleases and analysis of labeled spots by homochromatography-fingerprinting. Several examples of point mutations were detected. It was of interest that the 18S rRNA of Chinese hamster has more oligonucleotide sequences in common with those of man that rat or mouse.

Animals↗
PubMed1976

Time-blind analysis of TV-stored interviews. An objective method to study antidepressive drug-effects.

A new method of evaluating the time course of an antidepressive drug effect is described. It has the advantage that the rater is "blind" as to the duration of the treatment (time-blind analysis). TV-stored tapes of interviews recorded during the drug trial were presented in a randomized sequence to raters who ranked each patient's tapes in terms of the degree of depression shown during the interview. Patients treated with Amitriptyline showed a continuous amelioration of depression throughout the drug trial while those treated with Mianserin showed an amelioration of depression that was not constant in time.

Amitriptyline↗
PubMed1976

[Retrograde perfusion of the coronary sinus].

The investigations by Ratajczak-Pakalska showed peculiarities of cardiac venous circulation which make retrograde perfusion possible. Microcirculation is flowing in two directions, especially in diffuse cardiosclerosis. The results of the authors' animal experiments prove that the method of retrograde perfusion of coronary veins might be used ultimately in these cases.

Animals↗
PubMed1976

Detergent lysis for isolation of intact polysomes of Nivikoff hepatoma ascites cells.

An efficient, rapid procedure for the isolation of polysomes from Novikoff hepatoma ascites cells that avoids mechanical shear was developed, and Ivory was used as the detergent to lyse the cells. This method yields classes of large polysomes and 3- to 5-fold increased yields over that of routine homogenization procedures. Up to 70% of the polysome population is larger than tetramers. The polysomes and ribosomes are morphologically intact and contain undegraded ribosomal RNA and biologically active messenger RNA. This procedure also yields nuclei free of cytoplasmic contamination as a by-product of polysome isolation. These nuclei do not show evidence of protein degradation and are enzymatically active with respect to RNA polymerases I and II.

Animals↗