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Biomedical subjects

H Bouissou

Publications and source records attributed to H Bouissou.

At least 55 records · Page 3Linked to original sources
PubMed1985

[The fibroblast].

The fibroblast, major cell of connective tissue, secretes the various elements of interstitium: collagens, proelastin, glycoproteins and proteoglycans. It maintains the turnover of these structures and intervenes, also, in the cholesterol LDL metabolism. These various properties explains its different morphological aspects. In young patients, it is an active secretory cell. Its voluminous cytoplasm contains a well developed endoplasmic reticulum and others organelles. It is always in close connection with collagen fibers. The cytoskeleton consists of a fine network visible throughout the cytoplasm and near of secretory areas. In adult patients, the fibroblast keeps the same characteristics, but the endoplasmic reticulum is poorer than in young subjects. In old patients (physiologic or pathologic ageing) it becomes a quiescent cell. It is a flattened cell; its cytoplasm contains a poorly developed endoplasmic reticulum and numerous dense bodies. Its cytoskeleton is characterized by voluminous fascicles or bundles of microfilaments into large cytoplasmic areas. This modified fibroblast has not direct contact with collagen. In all cases, various stimuli can: activate the fibroblast. Then this cell becomes a large cell with very abundant reticulum endoplasmic, ribosomes, polysomes, and numerous secretory vesicles. It is an active secreting cell; becomes fibroblast and; change into myofibroblast by presence of myofilaments in its cytoplasm.

Actin Cytoskeleton↗
PubMed1985

[Anatomical study of Peyronie's disease].

An electron microscope study in the course of post-mortem examinations demonstrates the normal structure of the penis as a function of age. Twenty patients presenting with Peyronie's disease were examined in accordance with the same protocol. The lesions consisted of changes in the collagen fibers with inflammation, later followed by fibrosis, giving an idea of the stage of evolution of the disease. None of the etiopathological theories put forward to date provides a really satisfactory explanation of this mysterious disease.

Adult↗
PubMed1984

Fibroblast changes in cutaneous ageing.

With ageing there are progressive modifications in the connective tissue of the dermis. In ten young subjects the collagenous bundles are thick and the fibroblasts are active cells in close contact with collagen fascicles. In ten elderly subjects collagen fibres are fragmented and the fibroblasts are quiescent, without any contact with collagen. In ageing the most important lesion is the destruction of the relationship between fibroblasts and interstitial matrix. A role for fibronectin in this adhesion is suggested: in old subjects the papillary network of fibronectin is poorly developed. Furthermore, in the fibroblast we can see architectural changes in the cytoskeleton; this modification breaks up the cytoskeleton - plasma membrane - fibronectin unit and explains the secretory and metabolic changes observed in ageing, the dysfunction of the cell-interstitial matrix unit, and also the structural changes.

Adolescent↗
PubMed1984

Is cutaneous apoprotein B a better discriminator than serum lipoproteins for atherosclerosis?

Serum lipids, skin apoprotein B (apo B) and skin cholesterol measurements have been investigated in 2 populations: one with normal coronarography, the other with pathological coronarography. Within these 2 populations there were highly significant differences in serum apo B (P less than 0.001), skin cholesterol (P less than 0.01) and skin apo B (P less than 0.001) levels. Skin apo B is a valuable test because its increase is closely related to the coronary heart disease. From these 2 populations, 2 groups with normal serum apo B (less than 1.3 g/l) were selected and compared. No significant differences in the various serum lipids were observed except for triglycerides (P less than 0.05) and serum apo B (P less than 0.05). However, a very significant difference was noticed in the skin apo B (P less than 0.001). With this cutaneous apo B determination it was possible to foresee coronary heart disease in 75% of patients. This test can be therefore considered useful to predict coronary status.

Apolipoproteins B↗
PubMed1984

Dermis ageing.

On ageing major changes within connective components of the dermis can be seen: collagen looses its regular and fascicular appearance, while ground substance increases, elastic material decreases and the fibroblast cell population becomes "at rest". The resulting dermal ageing is different according to individuals and is related to genetic back ground and exposition to multiple aggressions. This review presents these changes of the superficial dermis and the role of fibroblasts in the conservation of the connective tissue integrity.

Adult↗
PubMed1984

[Cimetidine treatment of primary hyperparathyroidism].

Since Sherwood's report in 1979, contradictory findings have been published with regards to the treatment of primary hyperparathyroidism with cimetidine. We studied 16 patients with primary hyperparathyroidism treated with 1,200 mg of Cimetidine and followed-up clinically and biochemically. A decrease in serum calcium and urinary CAMP was observed after the fourth week of cimetidine therapy with no significant change in parathormone levels. In none of the cases did Cimetidine seem to provide a treatment of hyperparathyroidism, a condition which remains curable with surgery.

Calcium↗
PubMed1984

[Primary bilateral tubular adenocarcinoma with antecedents of invasive galactophoric carcinoma of the breast. Apropos of a case; review of the literature].

The authors report a case of primary bilateral adenocarcinoma of the fallopian tube in a woman with invasive ductal carcinoma of the breast in her antecedents. The tubal carcinoma was in situ or invasive and appeared as a bilateral hydrosalpinx, fact which often happens in the literature. Gross examination showed a tumoral mass of about 2 cm diameter at the fimbriated end of the right tube, the diagnostic of bilaterality requiring serial cuts on the left tube. The electron microscopic study showed ciliated differentiation in the neoplastic cells, bearing out the primitive nature of the proliferation.

Adenocarcinoma↗
PubMed1984

Determination of apoprotein B in the skin.

To determine the origin of cutaneous cholesterol a method has been developed for extracting and determining by electro-immuno-assay apoprotein B in the skin as already performed by Hoff at the arterial level. This study demonstrated the presence of apoprotein B in the skin and its quantification. The easy and reliable technique employed required only conventional laboratory instruments. Repeatability and reproducibility studies give encouraging results comparable with those obtained on arterial tissue. They show that at least one part of skin cholesterol originates in low density lipoproteins.

Apolipoproteins↗
PubMed1983

[Recurrent cerebral ischaemic attacks in young subjects. Arteriolar dysplasia or early atheroma?].

The case of a 33-year old woman with recurrent cerebral and peripheral ischaemic attacks is reported and the difficult nosological problem of juvenile ischaemia is discussed. Thromboangiitis obliterans (Buerger's disease) was excluded by morphological studies of finger tips, temporal artery and dermis, which demonstrated arteriolar dysplasia and premature aging of the connective tissue. A diagnosis of "juvenile atheroma" may be considered in view of the dyslipidaemia and increase in skin cholesterol and apoprotein B concentrations observed. A review of the literature, together with the biochemical and histological findings in this patient, suggests that ischaemic attacks, dysplasia of small vessels and serum dyslipidaemia reflected in the dermis are all manifestations of early atheroma.

Adult↗
PubMed1983

The effect of chronic beta-aminoproprionitrile intoxication on the periodontium of the rat. A light microscopic and histochemical study with review of the literature.

This histologic study of the periodontium of male white rats with chronic lathyrism demonstrates the appearance, progression, and persistence of certain pathologic changes caused by disordered collagen synthesis and maturation. Typical lesions were seen after 6 weeks. These include increased vascularity, especially in the periapical region, hyalinization and calcification of the transseptal fibers, alveolar bone and cementum resorption, interruption of the periodontal fibers with formation of typical hyaline zones surrounded by palisades of atypical fibroblasts, known as "lathyritic bodies," and an increase in mast cells.

Alveolar Process↗
PubMed1983

[The place of type I familial amyloid neuropathy in amylosis. Anatomo-clinical study of a case in France].

The clinical and pathologic findings of a case of amyloidogenic heredo familial neuropathy type I is presented. The patient is a man, died at 46 years. The examination of a nerve biopsy, the clinical evolution and the family history are representative of the condition. The study of autopsy material revealed amyloïd deposits within vessels walls, autonomic nerves of almost all viscera but liver, muscle and skin, around myofibers in the heart. Kidney glomeruli were markedly involved and numerous obsolescent glomeruli were seen. Lesions of central nervous system were of interest: enlargement of meningeal vessels walls due to amyloïd deposits as subpial deposition band were observed. The pattern of amyloïd deposits within peripherical nerves was quite different according to their proximal or distal ends. While distal amyloïd deposits were scant, nerve damages were striking. In proximal portions, amyloïdosis could be found in all layers of nerve structures including the walls of endoneural small vessels. Semi-thin sections allowed to observe rare myelinated fibers entrapped by aggregates of a microfibrillary material. While there was absence of a patent axonal lesion, clusters of regenerating fibers were not infrequent. Ultrastructural study confirmed the close relationship between amyloïd fibrils, Schwann cell basal laminae and collagen fibers. An immunopathological study using immunserums raised against the AA composant of amyloïdosis, K and lambda light chains, and prealbumin were negative. Our results are in agreement with descriptions already reported in the medical literature. Therefore, they allow to discuss already known pathogenic mechanisms for this rare affection transmitted by a dominant autosomic gene.

Adult↗
PubMed1982

[Localization of apoprotein B in human dermis. A study using optical and electronic immunoprocedures].

The structural localization of apo B, the major protidic constitute of low density plasma lipoproteins, was investigated in human dermis using an immunofluorescence procedure and an immunoperoxidase technique. Apo B was found attached to plasmic fibroblast membranes and along collagen fibers. Dermal areas containing apo B were more numerous in patients with coronary disease than in subjects of the same age with healthy coronary vessels. These results are consistent with those of biochemical determinations: concentrations of apo B in the aorta and skin are parallel and vary according to the severity of atheromatous lesions.

Apolipoproteins↗
PubMed1982

[Lathyrism and collagens I and III (author's transl)].

The administration of lathyrogenic agents (BAPN) at weak and prolonged doses (9 weeks, 1 g/kg/day) induced in the rat simultaneous alterations of aortic and cutaneous connective tissues comparable to the modifications observed during ageing in man. The lathyrogens produced this effect of inhibiting lysine-oxydase, the enzyme responsible for cross-linking collagen and elastin which leads to an increase in extractable collagen in the tissues. But BAPN did not appear to affect synthesis of collagen either in amount or in types I and III collagen ratio.

Aging↗
PubMed1982

Striae: morphological aspects of connective tissue.

The study of 15 abdominal striae in women aged 25 to 57 shows important histological modifications in the skin. The collagen is fragmented and the ground substance is abundant. Fibroblasts are globular, quiescent, and lose all signs of fibrillar secretion. In the light of the recent biochemical data, our results suggest that the striae are the consequence of fibroblastic dysfunction, due to abdominal distension. Comparison with scarred and normal skin indicate that striae are a special entity belonging to the group of connective dystrophies.

Abdomen↗