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Biomedical subjects

H Bernhardt

Publications and source records attributed to H Bernhardt.

At least 55 records · Page 3Linked to original sources
PubMed1988

[Diagnosis of anaerobic bacteria in bronchial secretions in pulmonary diseases].

In 50 bronchoscopic examinations the microflora of bronchial secretions has been investigated especially in order to detect anaerobic microbes with the use of the glove-box-method. In 19 patients we found the bronchial secretions steril (particularly carcinomas). Eight patients showed anaerobic gramnegative rods (Bacteroides and Fusobacteria) as predominant germs. Also in eight patients we found a mixed culture consisting in several anaerobic gram-negative rods, anaerobic gram-positive sporeless rods, anaerobic cocci, partly Clostridia and aerobes (carcinomas, pneumonias). A contamination can be excluded. In a series of patients anaerobes must be considered as infectious agent, in abscessed pneumonias particularly Bacteroides and Fusobacteria. Repeatedly an impressive clinical improvement has been established under the treatment by Metronidazol. The evidence of anaerobes especially succeeded in cases with copious mucopurulent or pure purulent secretions. The accumulation of anaerobes in bronchial secretions in alcoholics is evident.

Adult↗
PubMed1987

[Microecological regulative principles of human gastrointestinal flora].

In the course of coevolution of man and microorganisms the macroorganism must have developed mechanisms which allow a qualitative and quantitative regulation in his microbial colonization. On the several places, microbial growth is limited by various factors. Adherence ability of microorganisms belongs to the special colonization events. An uninhibited colonization begins if regulative factors are omitted. Factors of the host are only partially able to kill the microorganisms. Microbes possess signal receptors managing adaptation to environmental changes. If a strategy of growth and multiplication is not realizable such factors change to a strategy of survival. Very likely, these common microbiological principles are valid to the gastrointestinal tract, too.

Bacteria↗
PubMed1987

[Tests for anaerobes in patients with hemoblastosis].

A simplified control programme for the selective decontamination is proposed. Before the beginning of the attendance a microbial "large scale programme" is applicated in order to determine the quantitative and qualitative composition of the small intestinal and of the large intestinal flora on aerobic and anaerobic cultural conditions. During the attendance a "short-time programme" is applicated. At this on the basis of an aerobic cultural method and by the help of a direct microscopic numeration the flora is determined in its quantitative composition. At the end of an attendance the microbial "large-scale programme" is repeated.

Bacteria, Aerobic↗
PubMed1987

[Whipple's disease, an example of interactions between gastrointestinal flora and the macroorganism?].

Morbus Whipple is known as a systemic disease caused by bacteria and inducing the formation of mucopolysaccharides which is absorbed by macrophages. In a mucosal bioptate of a 60 years old patient suffering from this disease we could prove numerous rod-shaped bacteria (Propionibacterium) by electronmicroscopy. In the duodenal secretion aerobic and strictly anaerobic bacteria were to be found. Therapy with tetracycline was followed by a fast and distinct improvement of the clinical symptoms of the absorption disturbances but not so clear of the histological findings.

Digestive System↗
PubMed1987

[Simulation of intestinal microbial overgrowth by continuous culture].

By the aid of continuous flow culture of microbial populations it is possible to answer specific micro_ecological questions even under anaerobic conditions. We present some findings from a continuous flow model of human duodenal juice with microbial overgrowth. Germ counts and species were reproducible in a steady state over several days. Metabolic activity was very high. For example, this model allows the in vitro study of therapeutic possibilities against overgrowth syndrome, microbial fermentation of food, metabolism of drugs and the growth of microorganisms on surfaces in gastrointestinal tract.

Bacteria↗
PubMed1986

[Microbial pathogenesis of Whipple's disease].

In a case of Whipple's disease with electron microscopic demonstration of rod-shaped bacteria we could isolate Propionibacterium acnes (former name: Corynebacterium anaerobium), Actinomyces, and Lactobacillus from duodenal biopsies by the aid of modern anaerobic culture technique. Simultaneously we found a dysbiosis (increase of anaerobic coca and anaerobes) in du odenal juice. After antibiotic treatment Propionibacterium and Lactobacillus disappeared and an other type of dysbiosis (appearance of Bacteroides) was to be seen. In discussion is a change of immunologic state and following disturbed regulation of microbial colonization in the small bowel of patients with Whipple's disease.

Bacteria↗
PubMed1985

[Eumycetic mycetoma of the foot (maduromycosis)].

An eumycetoma pedis presenting as a circumscribed nodular form of maduromycosis in a 26-year old man from the People's Republic of Yemen is described. Clinical and light microscopic aspects of this "imported" deep mycosis are discussed. Histologic finding of a septate mycel permitted characterization of the microorganism as a eumycota.

Adult↗
PubMed1984

[Intestinal flora under conditions of stress].

In healthy probands influenced by endocrinic stress (application of ACTH, growth hormone, and triiodothyronine) the microflora of the content of the duodenum and of the feces is examined. After three weeks, only, rising numbers of bacteria could be detected in the duodenal content. The number of the aerobic bacteria, mainly of the coliforms in the feces, decreased whereas the number of the anaerobic germs increased. This stress model seems to be suitable for such researches.

Adrenocorticotropic Hormone↗
PubMed1984

[Growth of anaerobes of the upper small intestine using the glovebox technic].

With 80 patients a microecologic analysis of the duodenal flora has been performed. Due to the optimal cultivation technique with the glove-box more frequently as supposed till now, anaerobes with partial high numbers could be detected, and classified to different types of dysbiosis. A striking role among the isolated and classified gram-positive roots play the genera Eubacterium, Propionibacterium, Actinomyces, and Bifidobacterium. Peptococcus and Peptostreptococcus are to be found frequently, too. Furthermore the numbers of gram-negative anaerobes are high.

Bacteria, Anaerobic↗
PubMed1984

[Chemotherapeutic mixtures for the selective growth of anaerobes].

In order to find a selective medium for the cultivation of Gram-positive spore-less rods several chemotherapeutics have been tested. By adding of 5 micrograms metronidazol/ml to brain-heart-infusion-supplemented agar as well as by putting metronidazol discs on the agar it became possible to cultivate selective test tribes. Lincomycin (5 micrograms/ml), clindamycin (5 micrograms/ml) and novobiocin (5 micrograms/ml) inhibited almost all the test tribes. Spectinomycin (5 micrograms/ml) and neomycin (5 and 10 micrograms/ml) failed to inhibit. With 100 micrograms neomycin/ml Gram-positive anaerobes were clearly inhibited.

Anti-Bacterial Agents↗