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Biomedical subjects

H Adriaanse

Publications and source records attributed to H Adriaanse.

33 records · Page 2Linked to original sources
PubMed1990

Efficient recognition by rat T cell clones of an epitope of mycobacterial hsp 65 inserted in Escherichia coli outer membrane protein PhoE.

PhoE is a pore-forming protein, abundantly expressed in the Escherichia coli outer membrane. Previous investigations have shown the possibility of inserting antigenic determinants in cell surface-exposed regions of PhoE by recombinant DNA techniques without disturbing the biogenesis and the functioning of the protein. This method proved to be successful for foot-and-mouth disease virus B cell determinants. We have now shown for the first time that PhoE can also be used as a carrier molecule for T cell epitopes. A well-characterized T cell epitope (180-188) of the 65-kDa heat-shock protein (hsp 65) of Mycobacterium tuberculosis was expressed in PhoE and tested for recognition by specific T cell clones. Specific and efficient T cell proliferation was found after stimulation with this protein construct in vitro. Interestingly, paraformaldehyde fixation of antigen-presenting cells did not abrogate T cell recognition. Thus, in contrast to hsp 65 itself, recognition of epitope 180-188 in the context of PhoE appeared to be independent of antigen-processing events. At the level of polyclonal T cell responses the epitope in the context of PhoE is recognized more efficiently than 180-188 as synthetic peptide or in the context of the hsp 65 molecule itself. These findings indicate that PhoE may serve as attractive vaccine carrier not only for B, but also for T cell epitopes. Furthermore, the possibility for expression of PhoE constructs in attenuated Salmonella typhimurium strains offers the exciting prospect of new types of live oral vaccines expressing selected combinations of B and T cell epitopes.

Amino Acid Sequence↗
PubMed1990

Outer membrane PhoE protein of Escherichia coli as a carrier for foreign antigenic determinants: immunogenicity of epitopes of foot-and-mouth disease virus.

Outer membrane protein PhoE of Escherichia coli was used for the expression of antigenic determinants of foot-and-mouth disease virus. Five hybrid PhoE proteins were constructed containing different combinations of two antigenic determinants of VP1 protein of the virus. The hybrid proteins were expressed in two E. coli strains and the proteins were correctly assembled into the outer membrane. The inserted epitopes were exposed at the surface of the cell and were antigenic in this PhoE-associated conformation. Immunization experiments, performed with partially purified protein, resulted in all cases in a significant anti-peptide antibody titre. In one case in which the hybrid protein with the largest insert was used, a neutralizing antibody response was detected.

Amino Acid Sequence↗
PubMed1990

Protection of guinea-pigs against foot-and-mouth disease virus by immunization with a PhoE-FMDV hybrid protein.

A hybrid protein was constructed containing two antigenic determinants of the structural protein VP1 of foot-and-mouth disease virus, inserted in a cell surface-exposed region of Escherichia coli outer membrane protein PhoE. Immunization of guinea-pigs with partially purified protein resulted in high levels of neutralizing antibodies and complete protection against challenge with the virus.

Amino Acid Sequence↗
PubMed1989

Role of the cell surface-exposed regions of outer membrane protein PhoE of Escherichia coli K12 in the biogenesis of the protein.

To investigate the role of the cell surface-exposed regions of outer membrane protein PhoE of Escherichia coli K12 in the biogenesis of the protein, deletions were generated in two presumed cell surface-exposed regions of the protein. Intact cells expressing these mutant proteins were recognized by PhoE-specific monoclonal antibodies, which recognize conformational epitopes on the cell surface-exposed parts of the protein and/or were sensitive to a PhoE-specific phage. This shows that the polypeptides were normally incorporated into the outer membrane. When the deletions extended four amino acid residues into the seventh presumed membrane-spanning segment, the polypeptides accumulated in the periplasm. In conclusion, exposed regions of PhoE protein apparently do not play an essential role in outer membrane localization, which is consistent with the observation that these regions are hypervariable when PhoE is compared to the related proteins OmpF and OmpC. In contrast, the membrane-spanning segments are essential for the assembly process.

Amino Acid Sequence↗
PubMed1989

Alcohol consumption in France: production, consumption, morbidity and mortality, prevention and education in the last three decades.

This paper examines trends in the French consumption of alcohol over the last three decades, and includes data on the production of alcoholic beverages, the consumption of different types of alcoholic and non-alcoholic beverages, morbidity and mortality related to the consumption of alcohol, and a brief description of French preventive and educational measures in this area. In the last 15 years, the average alcohol consumption has ceased growing and even began to decrease. Data are given comparing consumption trends in several European countries, as well as more detailed data of alcohol consumption and related phenomena in France by region, by sex, by age and by socio-economic status (the last two categories for men only). Finally, a schema of the social dynamics of alcohol consumption is presented.

Alcohol Drinking↗
PubMed1989

Physicians' smoking and its exemplary effect.

Data presented are based on more than one hundred surveys on physicians' smoking, carried out in 31 countries since 1951. Prevalences by gender are shown in two figures. For 13 countries, it is possible to compare the trend in smoking prevalence among physicians with the trend among the general population. In ten countries, smoking prevalences decreased over time for male physicians and the general population. This suggests a positive influence of the physicians' example in those countries.

Epidemiologic Methods↗
PubMed1987

Use of outer membrane protein PhoE as a carrier for the transport of a foreign antigenic determinant to the cell surface of Escherichia coli K-12.

PhoE protein is an abundant transmembrane protein from the Escherichia coli K-12 outer membrane. A synthetic oligodeoxynucleotide corresponding to an antigenic determinant of the C-terminal part of the VP1 protein of foot-and-mouth disease virus was inserted into the phoE gene in an area corresponding to a cell surface-exposed region of the PhoE protein. The level of expression of the hybrid protein was normal and the protein was incorporated into the outer membrane. The VP1-epitope was exposed at the cell surface since intact cells were recognized by a monoclonal antibody which was raised against the virus.

Animals↗
PubMed1986

Smoking behaviour of Dutch general practitioners in the period 1977-1983.

Between 1977 and 1983 roughly half of the Dutch general practitioners were smokers. The percentage of smokers was decreasing, both among general practitioners and in the general Dutch population. The number of smoking male general practitioners was higher than for men in general and considerably higher than in the highest socioeconomic bracket. Among general practitioners the daily consumption of manufactured and handrolled cigarettes was lower while the cigar consumption was higher. In most countries physician smoking behaviour antedates that of the general population but in Holland general practitioners' smoking habits are about four years behind that of the general population. It seems that Dutch general practitioners attribute little value to the role of setting an example in health behaviour, especially where it concerns their own smoking habits.

Adult↗