Comparative scanning and transmission electron microscopical investigation of the medullo-spinal cerebrospinal fluid-contacting neurons.
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Biomedical subjects
Publications and source records attributed to H Adam.
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In the present study, 7 x 15 mm Cialit-preserved human tracheal segments were grafted to the tracheas of five 3-month old piglets. 72-120 days later, the animals were sacrificed and the grafts with proximal and distal control areas were removed. Tissues were then examined macroscopically and by light and scanning electron microscopy. We found that: (1) all grafts had taken well; (2) no animal suffered from clinical signs of respiratory obstruction or inflammation; (3) although luminal diameters of the tracheas increased from 8 mm to 22 mm, the size of the grafts remained unchanged but the resulting tracheal stenosis did not cause respiratory obstruction; (4) the graftet areas showed re-epithelialization. The nature of the re-epithelialization as well as the fate of the submucosa and tracheal cartilage will be reported in a subsequent publication.
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By the help of animal experiments at guinea-pigs, experimentally sensitized with potassium bichromate, is proved at the living biological model, that halothane results in a temporary blocking of the immunological function of the lymphocytes for the time of seven to ten days.
From thirty-two operated patients, narcotized with halothane, the PHA-stimulated rates of blastic transformation of cultivated lymphocytes were determined before and after the surgical operation. There was demonstrated a decrease of ability of the stimulation during 6--7 days. The maximum of this reaction was reached after 48 hours. We suppose that doses of halothane are inducing a decrease of immunodefence.
The soft palate and the tongue of adult Bombina variegata L. were studied by means of light and scanning electron microscopy. In the palate numerous taste-disks were found prominent by their polygonal surface structures. These are caused by longer microvilli at the margins of the different cells of the taste-disk or by deepened margins of the polygonal cells. The surfaces of the cells are slightly convex and are covered by numerous microvilli. In the tongue primarily fungiform papillae were observed (with the exception of the margins were taste-disks similar to those found in the palate were present). Their taste-disks also present polygonal structures, their epithelial cells are characterized by microridges while those of the epithelial folds surrounding the fungiform papillae own microvilli. As special formations within the taste-disks small knob-like protrusions between polygonal cells were found.
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A hitherto undescribed cell type within the fungiform papillae of the tongue of the toad, Bufo bufo (L.) and the taste-disks of the soft palate of Bombina variegata L. was identified by means of scanning and transmission electron microscopy. This cell type is characterized by an apical protrusion carrying numerous microvilli. Bundles of filaments are present in the cytoplasm of the protrusion. Vesicles with electron lucent content and microtubules are present thoughout the cytoplasm of the cell.
The angioarchitecture of the neural stalk and the encephaloposthypophysial portal system of the hypophysis of the toad, Bufo bufo (L.), was studied using three different methods. The neural stalk is mainly supplied by branches of the arteria infundibularis superficialis which form a widemeshed vascular network. Dorsally this network continues into the plexus of the pars nervosa. The vascularization of the pars nervosa is made up of the encephalo-posthypophysial portal system. This portal system consists of a hypothalamic branch (=portion), a mesencephalic and a mesencephalicbulbar branch (=portion). The hypothalamic branch was found to drain the pars ventralis of the tuber cinereum as well as more dorsal regions of the diencephalon. The mesencephalic-bulbar trunk enters the hypothalamic branch. The resulting common stem of the encephalo-posthypophysial portal vein the curves around the retroinfundibular communicating artery, crosses its ventral side and runs caudally. The secondary capillary plexus of the pars nervosa is characterized by well defined capillary plexus of the pars nervosa is characterized by well defined capillary networks which are located at the periphery of the parenchyma of the pars nervosa, thus forming a rostral, dorsal and ventro-caudal net. The central region of the parenchyma of the pars nervosa is supplied only by main branches of the encephalo-postpophysial portal vein. The venous drainage of the pars nervosa is via the vena hypophysea transversa.
The vascularization of the pars intermedia of the hypophysis of the toad, Bufo bufo (L.) was studied by injection of a mixture of India-ink and gelatine into the circulatory system of the head via the arteria carotis communis. Further methyl-methacrylate corrosion casts of brains were made and the hypophysial region of the corrosion casts was examined with the scanning electron microscope. The results showed that the vascularization of the pars intermedia of the toad hypophysis consists of a single-layered vascular network, which is located on the ventral surface of the pars intermedia. The network is formed by capillaries, which primarily run caudally in a fan-like manner and which show only a few cross-connections. In the rostral region of the pars intermedia this network lies rather superficially, while in the caudal region it slightly penetrates the parenchyma. The vascular network originates from vessels of the neural stalk and from wide capillaries of the rostro-ventral region of the neurointermediate junction. The venous drainage of the pars intermedia is exerted by veins, which leave the caudal region and drain into the veins leaving the venous pole of the pars distalis. The flat, wide meshed vascular net on the ventral side of the pars intermedia, demonstrated in this study, fits into the concept that the pars intermedia of the anuran hypophysis is under the control of nerve fibers coming from the hypothalamus.
Our technique of lumbar phlebography involves selective catheterisation of the lateral sacral veins with or without catheterisation of the ascending lumbar veins. Injection of these veins, in association with abdominal compression, produces good filling of the intra-spinal epidural veins, particularly at L5-S1 level. The anatomy and radiological appearances of the epidural veins are discussed. The phlebographic signs of disc herniation are presented. The diagnostic potential of this technique seems very promising in disc pathology, especially in the diagnosis of L5-S1 herniation and lateral prolapse at all levels.
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A transient close relationship between the chromatoid body and the developing acrosome is demonstrated in early spermatids of Myxine glutinosa.
The lumbar epidural veins are opacified by injection of the lateral sacral and ascending lumbar veins with abdominal compression. This technique provides a good opacification of the entire lumbar epidural venous system. Some anatomical points are discussed and clarified. The interest of the technique in the diagnosis of discal herniations is emphasized.