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Biomedical subjects

G Wolf

Publications and source records attributed to G Wolf.

At least 559 records · Page 31Linked to original sources
PubMed1986

[Ileus in enterothorax caused by right-sided rupture of the diaphragm. A case report].

The case described in this article shows an almost typical course with blunt abdominal trauma that occurred a long time ago, a primarily recognized but not surgically treated rupture of the diaphragm on the right side, and subsequently developing intestinal signs with recidivating attacks of jaundice caused by displacement of the liver with kinking of the bile duct. The relatively rare late complication of a right-sided enterothorax with ileus makes this a remarkable case and underlines once again the need for immediate or at least early surgery to avoid late complications.

Hernia, Diaphragmatic, Traumatic↗
PubMed1986

[Value of microscopic detection of acid fast rods in tuberculosis of the middle ear].

The microscopic identification of acid-proof rod-shaped bacilli via smear is of great importance for the diagnosis of tuberculous otitis media, since it is performed quickly and easily, for it enables an early diagnosis and treatment. The importance of the staining method using auramine is discussed, and it is examined if contamination by apathogenic or atypic mycobacteriae can yield a wrong positive result.

Adult↗
PubMed1986

[Radiologic breast diagnosis following breast-preserving operations and radiation].

The postoperative and post-irradiation changes occurring in the female breast that can be recorded via mammography, were demonstrated in 74 patients with carcinoma of the breast in the T1 and T2 stages after partial resection and postoperative irradiation. 47 of these women received exclusively percutaneous treatment, whereas 27 received combined treatment both percutaneously and interstitially. The changes occurring in the cutis and in the mammary gland tissue were analysed in respect of first and follow-up examinations. Problems of differential diagnosis were discussed and demonstrated.

Brachytherapy↗
PubMed1985

Developmental changes in gamma-glutamyl transpeptidase activity in nervous tissues with reference to amino acid transmission processes.

In homogenate supernatants of hippocampal formation and cerebellum of the rat, gamma-glutamyl transpeptidase (gamma-GTP) activities increased about 6 times from postnatal day 6 (0.178 +/- 0.02 and 0.187 +/- 0.007 U/g wet wt., respectively) to day 100. In dorsal root ganglia (0.183 +/- 0.003 U/g at day 6) and superior cervical ganglia (0.188 +/- 0.019 U/g at day 6) in which apparently amino acidergic transmission processes do not occur, enzyme activities were seen to go up 4 times and 2.4 times, respectively. Based on protein, enzyme activities in both brain material and dorsal root ganglia showed a similar pattern, whereas the activity increase in superior cervical ganglia was somewhat gradual (1.4 times from day 6 to day 100). Postnatal changes in gamma-GTP activities indicated a functional correlation with the maturation of amino acidergic structures. Kainic acid added to hippocampal extracts (0.45 and 5.0 mM) and, for comparison, to those of kidney (5.0 mM) did not yield any statistically significant effect on gamma-GTP activity.

Amino Acids↗
PubMed1985

[Surgical treatment of the isolated form of interrupted aortic arch].

We report on a 14-years old boy with isolated interruption of the aortic arch (type B). By the use of extracorporal circulation complete relief could be achieved by interposing a large Dacron tube. Follow up restudy demonstrated a normotensive patient without residual gradient of the systemic blood pressure.

Adolescent↗
PubMed1985

The structure of Artemia sp. (brine shrimp) haemoglobins. Purification of a structural unit to homogeneity.

The extracellular haemoglobins (Mr 260 000) of the brine shrimp Artemia sp. were cleaved by limited digestion with subtilisin. Structural units of Mr 16 000, which can bind dioxygen reversibly, were isolated. Analysis of the 16 000-Mr fraction (E) reveals the presence of a limited number of structural units. A single type of structural unit, E1 (Mr 15 800; pI4.8), was purified to homogeneity and characterized.

Amino Acids↗
PubMed1985

Histochemical demonstration of peptidases in the human kidney.

The localization of several peptidases in the human kidney was investigated histochemically. The membrane-bound peptidases, aminopeptidase A (APA), aminopeptidase M, gamma-glutamyltransferase (gamma-GT) and dipeptidylpeptidase IV, were mainly demonstrable in the brush border of the proximal tubule. In addition, APA was found in the glomeruli, while gamma-GT was found in the basal labyrinth of the proximal tubule. The lysosomal peptidases, dipeptidylpeptidase I and cathepsin B, were most strongly concentrated in the different-sized lysosomes of the proximal tubule, but they were also found in the small lysosomes of the distal tubule. Dipeptidylpeptidase II showed only a weak reaction in lysosomes of the proximal tubule. It is concluded that, in comparison with other previously studied species, the human kidney has a well-developed equipment with membrane-bound and lysosomal peptidases.

Adult↗
PubMed1985

Release of fibronectin is linked to tumor promotion: response of promotable and non-promotable clones of a mouse epidermal cell line.

By use of indirect immunofluorescence technique and enzyme-linked immunosorbent assay we show that JB 6 mouse epidermal cells have cell surface fibronectin (FN) and release FN into the culture medium. The addition of 10(-8) M 12-O-tetradecanoylphorbol-13-acetate (TPA) to promotable clones caused a 2-fold enhancement of the FN release over solvent control. On the other hand, in non-promotable clones, TPA in concentrations of 10(-8) M or 10(-7) M did not cause increased FN release. Mezerein, a non-phorbol diterpene and second-stage tumor promoter was also found to be active in causing enhanced FN release in promotable but not in non-promotable clones. The vitamin A derivative retinoic acid (RA) antagonized the TPA-caused FN-release in promotable clones. RA had, however, no effect on the basic release patterns, when given alone or given to non-promotable clones together with TPA. These results suggest that the increased release of FN may be a required event for promotion to transformation. Our view is derived from the observation that promotable clones of the JB 6 cell line release increased amounts of FN into their medium upon promoter exposure while non-promotable clones are unaffected.

Animals↗
PubMed1985

Studies on the tumour promoter-induced release of fibronectin from human lung fibroblasts, and its counteraction by retinoic acid.

The purpose of the experiments reported here is to improve our understanding of the mechanism whereby tumour promoters (e.g., 12-O-tetradecanoylphorbol-13-acetate, TPA) stimulate increased release of fibronectin (FN) from human lung fibroblasts (HLF) in culture. We investigated whether pretreatment of these cultures with a brief pulse of TPA would be sufficient to cause this effect, or whether continuous presence of TPA is required. We found that a pretreatment of 5-15 min with TPA caused the increased FN release when followed by a 2-h TPA-free incubation. The increased release did not cease upon removal of TPA. Longer exposure to TPA (1-2 h) also gave higher FN release than control cultures, but the effect was less pronounced. Pretreatment with retinoic (RA) for up to 30 min did not counteract the subsequent TPA effect after the RA was removed, even though we could show with labeled RA that it had entered the cells. Therefore, RA must be present simultaneously with TPA; it presumably acts on the cell surface when antagonizing the effect of TPA on FN release. When all cell-surface FN was removed by trypsinization and the cells were incubated with TPA, an increased release of FN into the medium occurred and a decreased accumulation of cell-associated FN compared to controls. These results suggest that FN is released even if the pericellular matrix is absent and that TPA interferes with the subsequent build-up of the matrix.

Cells, Cultured↗
PubMed1985

Experimental intracranial septic infarction: magnetic resonance enhancement.

Intracranial brain abscess was produced in three monkeys by embolization of a small pledget of polyvinyl alcohol (PVA) soaked in a broth of Staphylococcus aureus. Imaging of the chronic stable abscess was performed on the General Electric 8800 CT unit (Milwaukee, Wis.) and a 1.4 T superconducting small bore imaging system. Magnetic resonance imaging included saturation recovery, inversion recovery, and spin echo techniques. MR imaging was also performed after paramagnetic enhancement using gadolinium-DPTA (Gd-DTPA). Our results show that paramagnetic enhancement with T1-weighted imaging adds specificity and enables rapid assessment of abnormalities of the blood-brain barrier. T2-weighted imaging without paramagnetic enhancement was very sensitive in defining areas of abnormality in the brain but in our experiment lacked specificity. T2-weighted imaging with Gd-DTPA demonstrated no obvious change in the appearance of the lesion. The combination of T1-weighted Gd-DTPA and T2-weighted imaging appeared complementary in our experiment, and these images correlated well with the pathologic findings.

Animals↗
PubMed1985

[Tumor marking in the female breast--results--consequences].

The author reports on the results of preoperative tumour marking in case of occult changes in the female breast. His observations cover a period of eight years. 18 400 mammographic examinations were performed, and of these 86 occult carcinomas of the breast and 98 occult benign findings were treated surgically. Two changes which had been evaluated radiologically as probably benign--confirmation was obtained later by the course of the disease--must be rated as failures due to technical reasons. The author makes it a point to emphasise that the practising radiologist should not only describe suspicious occult changes which he sees in the mammogram, but should also underline the need for effecting preoperative marking. A localisation device for marking the occult change is absolutely mandatory. All clinically occult changes discovered in the mammogram must be followed by preoperative marking.

Adult↗
PubMed1985

[3 years' experience with concentrated autogenous fibrin glue in plastic ENT surgery].

The application of the fibrin tissue glue provides a better postoperative result, using skin grafts and flaps, because it provides an increased contact between wound area and graft, besides immediate revascularisation. A fibrin tissue glue was developed at the ENT Department of the University Clinic of Graz. Its fibrinogen is prepared from the patient's own blood, not from controlled donors as with the commercial fibrin glues. There is no danger of transmitting infectious diseases, or of immunereactions when using this autogenous tissue glue. Its production is easy and cheap. Three years of experiences with this concentrated autogenous fibrin glue in Plastic ENT Surgery are reported in this paper.

Drug Combinations↗