Biomedical subjects
Publications and source records attributed to G Wang.
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SNAP-25 is a synaptosomal associated protein localized at the plasma membrane of nerve terminals. SNAP-25 associates with syntaxin 1 and vesicle-associated membrane protein-2 (VAMP-2) and is thought to form a complex essential for neurotransmitter release. We have identified syndet, a novel protein related to the family of SNAP-25 isoforms. Like SNAP-25, syndet has regions with high probability of forming coiled coils, a cysteine rich-domain, and lacks a signal sequence or transmembrane domains. Syndet is tightly bound to membranes, possibly by acylation within the cysteine-rich domain. Syndet is expressed in non-neuronal tissues. In adipocytes, syndet is found at the plasma membrane and in an intracellular compartment. The identification of syndet supports the hypothesis that multiple SNAP-25 related proteins ensure specificity of vesicle fusion at the cell surface.
OBJECTIVE: To observe the effects of permissive hypercapnia on cardiopulmonary function in oleic acid induced acute lung injury (ALI) models. METHOD: ALI models of pigs were induced successfully by infusing oleic acid, Swan-Ganz cathether was used for monitoring hemodynamics and different low tidal volumes were set for variable hypercapnia. RESULTS: ALI was characterized by an increase in airway pressure and a decrease in static compliance (Cst), artery oxygen tension (PaO2), oxygenation index and cardiac output. There was also an increase in mean pulmonary artery pressure (mPAP), pulmonary vascular resistance (PVR), systemic vascular resistance (SVR) and shunt fraction. After using 0.98 kPa (1 kPa = 10.2 cmH2O) PEEP, some of cardiopulmonary functions were improved. While reduced tidal volume to 7.7 +/- 0.3 ml/kg and PaCO2 increased to 9.44 +/- 1.27 kPa (1 kPa = 7.5 mmHg), which caused neither a change in PaO2/FiO2, PaO2, Cst nor in SVR, PVR but an increase in cardiac output. Though ventilation setting did not change except for the reducing tidal volume to 6.1 +/- 0.6 ml/kg and PaCO2 reached 12.1 +/- 1.05 kPa, then various adverse effects on cardiopulmonary system were observed. CONCLUSION: It seems that mechanical ventilation with permissive hypercapnia was safe at certain level.
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Cultures of rat lung microvascular endothelial cells (RLMECs) were obtained from perepheral lung tissue. The lung tissue was cut into small pieces and cultured with RPMI-1640 containing 20% bovine calf serum, 90 micrograms/ml heparin, 4 mmol/L L-glutamine, 100 mu/ml penicillin and 100 micrograms/ml streptomycin. Erythrocytes and leukocytes left the tissue first, followed by RLMECs. Fibroblasts and other cells grew after 72 hours of culture. After 60 hours of culture, the lung tissue was discarded. RLMECs in flask showed regular cobblestone morphology and positive for binding of the lectin Bandeiraea simplicifolia I and indirect immunofluoresence staining with factor VIII antiserum.
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The present study investigated whether IL-1 and IL-6 involved in the control of proliferation in cultured anterior pituitary (AP) cells. Primary culture of AP cell were obtained from either male and female Sprague-Dawley rats. The effects of IL-1 and IL-6 assessed by [3H]-thymidine incorporation rate. It was found that: (1) IL-1(1-100 ng/ml) stimulated 3H-TdR incorporation into AP cells of male and female rats. (2) The lower concentration of IL-6 (0.1 ng/ml) inhibited 3H-TdR incorporation rate, but higher concentration of IL-6 (1-10 ng/ml) stimulated 3H-TdR incorporation into AP cells of male rats. (3) IL-6 (0.1-10 ng/ml) stimulated 3H-TdR incorporation into AP cells of female rats. These results indicated that IL-1 and IL-6 can modulate the endocrine activity as well as the proliferation of AP cells.
OBJECTIVE: To observe the effects of Tongxinluo (TXL) capsule on hemodynamics and myocardial oxygen consumption in dogs. METHODS: The coronary arterial arterial blood flow, cardiac output, left ventricular pressure, blood pressure, myocardial oxygen consumption of dogs were recorded by the polygraph system before and after intraduodenal administration of TXL. RESULTS: TXL 0.72 g/kg increased obviously the coronary arterial blood flow by 21.56% after administration, and the left ventricular work was increased simultaneously, the difference was significant (P < 0.01) as compared with the control group. CONCLUSIONS: TXL capsule could increase obviously the coronary arterial blood flow, dilate the coronary vessels, improve myocardial blood supply, increase left ventricular work, and result in the enhancement and readjustment of the "pump" function of heart and improvement of the intravascular stasis condition of blood circulation.
OBJECTIVE: To study the effects of Tongxinluo capsule (TXLC) on experimental myocardial ischemia and infarction, arrhythmia and hyperlipidemia. METHODS: Using the methods of epicardial mapping and NBT-ink double staining and the ratio of tissue in myocardial infarction in which the anterior coronary artery was ligated in anesthetized dogs, acute myocardial ischemia was induced with pituitrin and hyperlipidemia was induced with feeding high lipid diet in rats, arrhythmia was induced with chloroform in mice. RESULTS: The TXLC reduced significantly the myocardial ischemic limits, degree and the myocardial infarction area, inhibited obviously the ocurrence of arrhythmia and reduced the level of hyperlipidemia. CONCLUSION: The TXLC could improve significantly the myocardial ischemia mycardial infarction and prevent obviously the ocurrence of arrhythmia and hyperlipidemia.
Synovial sarcoma in the head and neck with 3 cases are reported. Some clinical features and experiences of surgical excision are analysed according to our materials and review of the literature. We believe that the key to prevent synovial sarcoma from recurrence and matastasis is to avoid local excision, and pay more attention to this disease before operation. It is not sensitive to radiation therapy.
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OBJECTIVE: To examine the genomic status and the mRNA expression of the nm23-H1 gene, and to analyse the relationship between metastasis of non-small cell lung carcinomas and the gene abnormalities. METHOD: By using PCR-SSCP and semiquantitative RT-PCR, the nm23-H1 gene mutation and mRNA expression were studied in 31 cases of non-small cell lung cancer. The normal tissue adjacent to carcinoma and normal lung tissue were used as control. RESULT: None of the nm23-H1 gene mutation was found in all the lung cancer samples. Decreased expressions of this gene were found in 14 of 20 lung cancer cases with lymph node metastasis, the rate (14/20) of this was significantly higher than that (3/11) of the non-metastatic cancers (P < 0.05). CONCLUSION: These data indicate that nm23-H1 may be a putative metastasis suppressor gene which had shown reverse regulating activation in the metastatic progression of lung cancers, and the level of nm23-H1 mRNA may be considered as one of the pathological indicators in predicting metastatic potential of lung cancers.
52 adult patients with CT-documented fourth intraventricular hemorrhage (FIVH) were treated. The various etiologic findings included intraparenchymal hemorrhage with secondary FIVH (24 cases), spontaneous subarachnoid hemorrhage (8), spontaneous IVH (12), and trauma (8). Of the 52 patients, 28 died because of hemorrhagic dilation of the fourth ventricle in spite of aggressive therapy. Of the 24 FIVH patients without dilation, 10 died and 14 survived. The survival rate for the patients with hemorrhagic dilation of the fourth ventricle was significantly lower than that for FIVH patients without dilation. Of the 28 patients with FIVH associated with dilation, 24 presented with a Glasgow Coma Scale (GCS) score of less than 5 or 5, 2 with a GCS score of 7, and 2 with a GCS score of 13. In the 24 FIVH patients without dilation, 8 presented with a GCS score of 3 to 5 (seven died and one survived), 4 with a GCS score of 6 to 8 (two died and two had survival), 5 with a GCS score of 9 to 12 (one died and four had survival), and 7 with a GCS score of 13 to 15. There was a great difference of GCS scores between the two groups. Logistic regression multivariate analysis demonstrated that hemorrhagic dilation of the fourth ventricle was the most significant outcome predictor, followed by GCS score and then the presence of diffuse IVH.
OBJECTIVES: To observe the clinical features and the effects of management of juvenile-children type of Coats' disease and to approach the classification of the disease. METHODS: Fundus was examined and fundus fluorescein angiography (FFA) was made. Laser photocoagulation and cryotherapy were used for treatment. RESULTS: Compared to adult type of Coats' disease, this type was characterized by early onset, severe macular lesion, rapid progression and poor visual prognosis. The vision in 75.0% of eyes was unchanged, and in 19.2% of eyes was improved after treatment. CONCLUSIONS: Visual damage is more severe in this type. Early examination and early treatment are the key points to decrease visual deterioration. The classification of the disease is helpful in understanding the disease.
Studies on the expanded eradicative and modified operation for treatment in patients of late pancreas head cancer, so as to enhance the incised rate, survival rate and life quality. Elective laparotomy was done in 20 patients. Expanded eradicative operation was performed in 18 patients (portal vein excision 19, left outer leaf of liver 8, transverse colon 3). We have reconstructed the portal vein 6, modified the Whipple operation, reconstructed the elementary tract using Roux-Y interposed jejunum with orientatied intussusception of artificial papilla. The incised rate of expanded eradication was 90% without postoperative serious complications such as biliary and pancreatic leakage, massive hemorrhage and operative death. The longest survival time was 5 years. The two-year survival rate was 50%. Statistical analysis (t test and x2) showed signithicant differences (P < 0.001).: (1) The exsion of original carcinomous focus may relieve the patient's symptoms and can prevent portal vein from carcinomous cell infiltration. (2) After enbloc incision with metastatic focus, sequential treatment could be given successfully.
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To explore the influence of displacement and hypoplasia of nasal cartilages to cleft lip nose, 38 patients with secondary cleft lip nasal deformity were randomly chosen. Measurement of length and width of the nasal cartilages was made, and places of them were inspected. The displacement of the cartilages was found in each nose, and hypoplasia of cartilages was in those noses whose deformities were more severe. The conclusion is that both displacement and hypoplasia of nasal cartilages are causing factors of nasal deformity.
To study the mutagenicity and carcinogenicity of Nabumetone, we conducted Ames test (TA97, TA98, TA100, TA102), micronucleus test(MN) in mice marrow, chromosomal aberration assay(CA) in CHL cells in vitro, CA in germ cells from testes of mice, and cell transformation test of Syrian hamster embryo(SHE) cells. The maximum concentration was 500 micrograms/plate in Ames test with and without S9 mix. The mice were treated orally(gavage) daily for 4 days in 3 doses in which the maximum dose was 60% LD50 and sampled at the 5th day in MN. The maximum concentration was the dose that the growth of 50% of cells was inhibited in CA of CHL. Cells were harvested after recultured for 18 hours in fresh medium after treatment for 6 hours in the test with S9 mix, and after treatment for 24 or 48 hours in the without S9 mix. The mice were treated orally(gavage) daily for 5 days in 3 doses in which the maximum dose was 1/4 LD50 and sampled at the 6th day in CA of germ cells from testes of mice. 2 micrograms/ml was chosen as the maximum concentration in the cell transformation test of SHE cells, and result was observed after treatment for 9 days, All the tests obtained the same negative result as that reported by other investigators.