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Biomedical subjects

G Vasquez

Publications and source records attributed to G Vasquez.

At least 55 records · Page 3Linked to original sources
PubMed1986

Histologic and ultrastructural changes in human endometriotic implants treated with the antiprogesterone steroid ethylnorgestrienone (gestrinone) during 2 months.

In 20 infertile patients, the laparoscopic, histologic, and ultrastructural characteristics of endometriotic implants were studied before and after a 2-month course of therapy using 1.25 mg of the antiprogesterone steroid ethylnorgestrienone daily. Before treatment, well-differentiated endometriotic foci were seen only in implants with a rich microvascular supply. It is postulated that the degree of microvascular development may determine the differentiation of ectopic endometrium. After therapy, well-differentiated endometriotic tissues were recovered in only one patient. In all other patients, the foci remained undifferentiated, while in 11 patients striking epithelial involutionary changes were seen, such as nuclear pyknosis, extrusion of nuclear remnants, enhanced lysosomal autophagy, abortive secretion, and focal epithelial erosion. These histologic and ultrastructural changes reflect a progesterone withdrawal effect in endometriotic implants. It is thus concluded that ethylnorgestrienone induces inactivity in most endometriotic implants, while marked involution is observed in many foci. However, this subcellular involution does not result in a change of the extent of the disease according to the American Fertility Society classification system. Preliminary data concerning the pregnancy rate reveal that five patients conceived during a mean follow-up period of 6 months.

Cell Differentiation↗
PubMed1985

The response of human endometriotic implants to the anti-progesterone steroid R 2323: a histologic and ultrastructural study.

The histology and ultrastructure of small endometriotic lesions were studied in 19 patients before and after hormonal therapy with the anti-progesterone steroid R 2323 (Gestrinone). Histologic results demonstrate that treatment of endometriosis with this steroid does not result in complete elimination of the endometriotic foci, although glandular proliferation and secretion are arrested in most implants. The ultrastructural results indicate that this inhibition of proliferation and secretion is related to an enhanced activity of the lysosomal system in the epithelial cells of some endometriotic foci. In other implants, or even in other cells of the same foci, epithelial cells with only a small amount of supranuclear cytoplasm but lacking lysosomes may be found. The morphologic data demonstrate that the cellular involutionary response to the antiprogesterone drug Gestrinone involves an activation of the lysosomal system, an abortive apocrine secretion of cell remnants and finally, in some implants, an extrusion of individual epithelial cells. Since this involutionary process of endometriotic cells mimics the pre-menstrual lysosomal degradation in the endometrium, it is suggested that the competitive binding of the antiprogesterone Gestrinone to the progesterone receptors of endometriotic epithelium may cause a cellular progesterone withdrawal effect.

Adult↗
PubMed1984

Peritoneal endometriosis: scanning electron microscopy and histology of minimal pelvic endometriotic lesions.

In 36 patients with laparoscopically diagnosed endometriosis, biopsies were taken from different areas of the pelvic peritoneum bearing foci of endometriosis. The biopsies were studied by scanning electron microscopy and by light microscopy. Combined use of these techniques resulted in the differentiation of three topographically and morphologically different types of endometriotic lesions: intraperitoneal endometriotic polyps with no glandular openings but associated with deeper endometriotic glands and stroma; intraperitoneal endometriotic foci with surface epithelium, glands, and stroma; and retroperitoneal small lesions with few glands and scant stroma. The morphologic features of endometriotic foci indicate that they do not follow the typical cyclic changes described for the uterine endometrium. Our microanatomic characterization of endometriosis is discussed in relation to the conflicting data concerning peritoneal fluid constituents and infertility in patients with minimal endometriotic lesions.

Biopsy↗
PubMed1983

Tubal mucosa and ectopic pregnancy.

Fallopian tubes containing a gestation are frequently normal on macroscopical and gross histological examination. Scanning electron microscopy and light microscopy studies of tubal biopsies taken from five groups of women showed marked differences in the ciliated surface which was measured planimetrically on photographs. The proportion of ciliated cells was significantly lower in biopsies taken from 25 women with tubal pregnancies compared with that in biopsies from seven women with intrauterine pregnancies at the same gestation. Marked deciliation was also seen in eight women biopsied during tubal surgery sometime subsequent to an ectopic pregnancy and in four women biopsied during tubal surgery who subsequently had a tubal pregnancy when compared with a group of women biopsied during tubal sterilization.

Cell Count↗
PubMed1983

The epithelium of human hydrosalpinges: a light optical and scanning electron microscopic study.

Multiple tubal biopsies from 21 patients with thin-walled dilated hydrosalpinges and from 12 patients with thick-walled fibrous hydrosalpinges were studied. All the biopsies were examined by light microscopy and scanning electron microscopy and the ciliated surface area at multiple locations measured by planimetry. These biopsies were compared with control biopsies taken from 13 normal women at varying stages of the menstrual cycle.

Adult↗
PubMed1981

The evolution of experimentally induced hydrosalpinges in rabbits.

Damage to fallopian tubes caused by hydrosalpinges is a major reason for persisting infertility. We describe the mucosal changes that occur following induction of experimentally induced hydrosalpinges in 25 rabbits. Ampullary biopsies obtained at varying intervals were examined with light microscopy and scanning and transmission electron microscopy. Mucosal folds showed progressive atrophy. The epithelium was flattened. The population of ciliated cells decreased and, finally, deciliation became generalized. The morphology of nonciliated cells changed from an initial high content of granules of secretion to a final stage of vacuolated cytoplasm. Cell desquamation was common.

Animals↗
PubMed1980

Tubal lesions subsequent to sterilization and their relation to fertility after attempts at reversal.

Tubal biopsy specimens were obtained at the time of tubal anastomosis in 26 previously sterilized women. Scanning electron microscopy showed that half the patients had abnormalities of tubal mucosa, including loss of mucosal folds, deciliation, and polyposis. These pathologic conditions, the morphologic features of which we describe, significantly increase in incidence the longer the time after sterilization. Our evidence suggests that the sooner reversal is performed after sterilization the greater the change of a successful intrauterine pregnancy.

Adult↗
PubMed1976

Fimbrial microbiopsy.

Microbiopsies from the fimbrial surface were obtained from 70 women to determine the percentage of ciliated cells. The following observations were made: 1. There is a close correlation between the percentages of ciliated cells in each of the two oviducts; 2. The percentages of ciliated cells in normal, fertile women vary between 54 and 70 with no apparent variation between the follicular and luteal phases of the cycle; 3. The percentage of ciliated cells is reduced in hypoestrogenic amenorrhea, during pregnancy and during steroid contraception; 4. Ovarian endometriosis without tubal involvement is usually not associated with fimbrial reciliation, but sequelae of pelvic inflammatory disease can be associated with deciliation of the fimbrial surface; 5. Two cases are reported in which the fimbrial surface showed extreme deciliation in association with sqamous metaplasia of the epithelium, possibly indicating defective ovum transport over the fimbrial surface.

Adult↗
PubMedDate not supplied

[Subrenal coarctation of the aorta. Presentation of a clinical case].

Coarctation of abdominal aorta constitutes a rare group of vascular abnormalities, including segmental stenoses and extended hypoplasia below the restriction. Usually hypertension is the only clinical evidence. The natural history of the surgically untreated disease foresees a decline of life expectancy; while surgical operation permit an almost complete "restitutio ad integrum". The Authors report a case of coarctation of the abdominal aorta come to their observation whose particularly was determined by the absence of high blood pressure, the aortic stenoses being located under the renal arteries.

Aorta, Abdominal↗
PubMedDate not supplied

Vasoepididymostomy to the head of the epididymis: a comparative study of two microsurgical techniques in the rabbit.

In an experimental animal study, two different microsurgical techniques of vasoepididymostomy to the caput epididymidis are compared: an end-to-side anastomosis with the epididymal tubule and an end-to-end anastomosis with a section of a cluster of the epididymal tubule. The patency rates are respectively 50 per cent and 26.3 per cent, as controlled by deferentography and histology. Examination with scanning electron microscopy, shows that the superiority of the first method is due to the better apposition of both mucosas .

Animals↗
PubMedDate not supplied

Microsurgery in gynecology.

Microsurgical techniques have changed the outcome of infertility surgery. Tubal anastomosis for reversal of sterilization has resulted in pregnancy rates of up to 65%. In cases of salpingostomy, the ectopic pregnancy rate is quite high. In ovarian microsurgery, postoperative adhesions can be avoided and a 54% pregnancy rate achieved with endometriosis surgery.

Endometriosis↗
PubMedDate not supplied

[Video-laparoscopic splenectomy: experience in 3 cases].

BACKGROUND: In selected cases the spleen can be removed: METHODS: 2 women and 1 men with immune thrombocytopenic purpura, with hypersplenism without splenomegaly, underwent splenectomy by laparoscopic means. RESULTS: In 2 cases the splenectomy has been performed entirely by laparoscopic means. In 1 case the operation has been converted as we have not been able to control a venous bleeding from injured hilar spleen vessels. DISCUSSION: No particular technical problems arose in the 2 cases entirely conducted by laparoscopic means. Care must be taken manipulating the hilar vessels as the veins are fragile and their bleeding is difficult to be controlled in laparoscopy. The laparoscopic splenectomy seems to offer less postoperative pain, shorter hospitalization and improved cosmesis according to our previous experience with open splenectomy. CONCLUSIONS: The good result of the operations encourages the laparoscopic approach to splenectomy, in selected cases.

Adolescent↗
PubMedDate not supplied

[Deep venous thrombosis and surgery. Assessment of the main risk factors].

Deep venous thrombosis is a quite frequent pathology with a high degree of morbidity and death-rate. Two are the main problems of this disease: the risk of pulmonary embolism and outcome of a post-thrombotic syndrome, leading patients to infirmity. The patients max exposed to the risk of deep venous thrombosis are the surgical ones: the authors, with a retrospective study on 480 surgical patients, examine the risk factors mostly related with the onset of deep venous thrombosis. At the end they refer about connection between the risk factors studied and the clinical display of in surgical patients.

Adult↗