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Biomedical subjects

G Turowski

Publications and source records attributed to G Turowski.

At least 109 records · Page 6Linked to original sources
PubMedDate not supplied

Soluble HLA class I antigens in serum of healthy individuals--population study.

Variability of concentrations of s-HLA-I depending on allelic specificity substantiated realisation of research in population of 248 healthy, unrelated individuals. Defined phenotypes from tissue typing of polymorphic HLA complex enabled concentrations measurements of 1553 serum samples for HLA--A, B, and C loci determined antigens. Semi-quantitative technique of inhibition microcytotoxic reaction according to Tait et al. (1981) and Mclean et al. (1983) with usage of policlonal sera anti-HLA was applied. For most of numbers of the allelic specificity the concentration of antigen material in soluble form (s-HLA-I) in blood serum were nominally very high and high. For certain numbers of specificity e.g. HLA--A26, A29, B39, B52, B56, Cw5, Cw6 the percentage of sera, where the s-HLA concentrations were decreased was observed. The results were presented as mean values of inhibition microcytotoxic reaction--according to NIH classification. Authors point on usefulness of results for s-HLA comparative analysis of particular HLA allelic specificity, specific for certain diseases e.g. Cw7 for SNHL patients and B27 for ankylosing spongilitis.

Adult↗
PubMedDate not supplied

HLA class I haplotypes in families of children with coeliac disease.

The purpose of the study was the analysis of HLA-AB haplotypes frequency in the families of children with coeliac disease. Haplotypes present in 46 probands' families including 69 affected children, 49 healthy siblings and 91 parents were verified. HLA antigens were typed by Terasaki and McClelland's routine two-step-microcytotoxic assay in NIH modification. Among 138 haplotypes, the following were significantly more frequent in affected children: HLA-A1-B8 (3116 x 10(4)), HLA-A3-B8 (290 x 10(4)) and HLA-A2-B8 (217 x 10(4)). Total frequency of haplotypes including HLA-B8 antigen in comparison to control population equalled 3986 x 10(4) vs. 763 x 10(4). HLA-A1-B8 haplotype frequency was twice lower in probands' healthy siblings and parents, equalling 1837 x 10(4) and 1868 x 10(4), respectively. Highly significantly more frequent HLA-A1-B8 haplotype found in probands' families may indicate the correlation between inherited gene products and increased risk of coeliac disease incidence.

Case-Control Studies↗
PubMedDate not supplied

HLA class I antigens in families with coeliac disease.

The aim of the study was the analysis of the frequency of HLA class I antigens in the Polish population of children suffering from coeliac disease and their families, as well as the estimation of the relative risk of incidence, etiologic fraction (EF) and preventive fraction (PF) indexes. Forty-six probands' families were included in the typing: 69 children with coeliac disease confirmed clinically and histologically, 49 healthy siblings and 91 parents. The HLA antigens were typed with routine Terasaki an McClelland's two-stage microcytotoxic assay in NIH modification. The following antigens occurred significantly more frequently (p < 0.0000...1) in phenotypes of children with coeliac disease: HLA-A1 (chi 2 = 35.90; RR = 4.3; EF = 0.44), -B8 (chi 2 = 88.20; RR = 8.8; EF = 0.58) and Cw7 (chi 2 = 55.24; RR = 7.5; EF = 0.69). The positive correlation for the specificity of HLA-A1, -B8 was proved also in siblings (chi 2 = 16.03; chi 2 = 18.10) and parents (chi 2 = 15.67; chi 2 = 32.67). The presence of antigens HLA-A1, -B8 in the phenotype may be the risk factor predisposing for the manifestation of hypersensitivity to gluten.

Adult↗
PubMedDate not supplied

Metabolic activity of neutrophils in patients suffering from chronic viral hepatitis.

The aim of the study was to evaluate the functional state of peripheral blood neutrophils in patients with chronic active viral hepatitis. Twenty-six patients with HBV, HCV or CMV in different clinical status were included in the analysis. In the study, the number of leukocytes and neutrophils was determined. The metabolic activity of neutrophils was examined in NBT reduction tests i.e. spontaneous (NBTsp) test and the one stimulated with LPS E. coli (NBTst). The results were analysed in relation to disease advancement and the type of viral infection. The data obtained from the affected patients were compared with the results from 46 healthy subjects. Most patients displayed neutropenia. It was found that the number of NBT positive cells and the coefficient of neutrophil metabolic activity (CNMA) in NBT sp test were highly significantly increased. The majority of patients had reduced values in NBTst test, which suggested lack of response to LPS E. coli by neutrophils. These findings may reflect the state of inflammation in the body. NBT reduction tests may be useful in monitoring metabolic activity of neutrophils in patients with chronic active viral hepatitis.

Adult↗
PubMedDate not supplied

Lymphocytotoxines frequency and HL-A typing in "small for date" children.

Nineteen mothers and their children, aged from 1--24 months born as "small for date" (SFD) were investigated. The lymphocytotoxicity test performed in the SFD mothers showed in comparison with the control group differences near the borderline of statistical significance. HL-A-1, or A-l with A-10 was more often found in SFD, than in control children.

Antilymphocyte Serum↗
PubMedDate not supplied

Studies on the behavior of lymphocytes in the direct lymphocytotoxicity test (DLT) in surgical patients: I. Diagnostic value of the test.

The results of the investigations of immunological reactivity in 120 surgical patients are presented. The direct lymphocytotoxicity test (DLT) was used. Negative results were found in only 20 patients; in the remaining 100, the number of damaged lymphocytes in the peripheral blood, examined in vitro was dependent on: 1) the type of surgical procedure (e.g. open reduction and internal fixation of long-bone fractures), 2) severity of surgical disease, 3) interaction of drugs used, and 4) infection, most probably viral. A high percentage of damaged lymphocytes was found firstly, in patients suffering from severe wound infections; secondly, in those with disorders of the peripheral arteries during the postoperative course and thirdly, in patients with advanced malignancy of the alimentary tract. The results of these investigations--regarded by the authors as a preliminary report--indicate that the DLT could be introduced routinely as part of pre- and postoperative examinations of surgical patients in order to evaluate their immunological reactivity.

Adult↗
PubMedDate not supplied

Studies on the behavior of lymphocytes in the direct lymphocytotoxicity test (DLT) in surgical patients: II. Dynamics of immunological reactivity in selected cases.

The results of investigations on the dynamics of immune reactivity are presented in 6 cases selected from 38 surgical patients. The investigations were carried out during surgical treatment of peripheral arterial diseases, malignant neoplasms of the alimentary tract, and extensive chronic inflammatory processes of the soft tissues. The evaluation of immune reactivity of these patients was based on the results of the direct lymphocytotoxicity test (DLT), the intensity of the autolymphocytotoxic reactions in the cold (15 degrees C) test, morphology and other changes of lymphocytes (various forms, lysis, leukergy) and on their ability to undergo blastic transformation in vitro after stimulation with PHA. The joint evaluation of investigations carried out repeatedly in these diseases may be useful for tracing the dynamics of immune reactivity in surgical patients.

Adult↗
PubMedDate not supplied

ABO blood groups system in laryngeal cancer patients and after survival of 3 and 5 years.

The analyzed material comprised 200 patients with cancer of the larynx with various locations of the tumour and patients surviving 3 and 5 years after operation. The frequency of ABO blood groups was analysed separately in cases of epiglottis cancer (n = 153) and glottis cancer (n = 37). The frequency of A1, A2, B, A1B, A2B, O blood groups was compared with that in 22,422 healthy individuals living in South Poland. The A2B blood group was significantly more frequent in patients with epiglottis cancer and in those with laryngeal cancer. The difference was not significant after survival of 3 and 5 years.

ABO Blood-Group System↗