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Biomedical subjects

G Stein

Publications and source records attributed to G Stein.

At least 415 records · Page 23Linked to original sources
PubMed1975

Separation of human lymphoid cells by preparative cell electrophoresis.

By means of free-flow electrophoresis a preparative separation of cells according to their surface charge is achieved. The method has been applied to human lymphocytes of blood and of tonsils. Technical details are described and discussed with respect to optimal separation conditions, reproducibility of results and viability of the separated cells. Unseparated cells, pooled separated cells and cells corresponding to different electrophoretic mobilities are compared for stimulation in response to PHA, determination of cells bearing membrane immunoglobulins, colony growth of hemopoietic stem cells and DNA syntheses in vitro. It is concluded that reproducible results can be obtained, that the biological activities of the cells after separation are unaltered and that a separation or at least an enrichment of cells with certain properties can be achieved.

Adenoids↗
PubMed1974

Reduction of ferricytochrome c by some free radical agents.

Fast pulse radiolysis and kinetic spectroscopy were used to rapidly generate a variety of free radicals in situ and study their reactions with ferricytochrome c in the time range 10(-6) to 1 second. The radicals included t-butanol, which is inert to ferricytochrome c; malate, lactate, and ethanol, which react with it relatively slowly but are completely utilized in reducing it to ferrocytochrome c; and hydrated electrons and hydrogen atoms, which react with it very rapidly but yield ferrocytochrome c only in part, showing intramolecular consecutive reactions and further attack on the ferrocytochrome c protein. From a detailed comparison between malate and hydrogen atoms it is argued that malate reacts directly and selectively with a specific part of the ferricytochrome c surface while hydrogen atoms react with other parts of the protein too, yielding radicals which in part transfer intramolecularly to yield ferrocytochrome c.

Butanols↗