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Biomedical subjects

G Singh

Publications and source records attributed to G Singh.

At least 739 records · Page 41Linked to original sources
PubMed1983

Histocompatibility in cardiac transplantation with particular reference to immunopathology of positive serologic crossmatch.

Cardiac allografting was carried out in 33 patients during the past 2 years. Twenty-one (64%) of the patients are alive and others lived for different periods after transplantation. The number of HLA-AB and HLA-DR antigens matched or mismatched was not significantly different between the surviving and deceased patients. However, 100% (4 of the 4) of the patients with a positive serum crossmatch with donor T lymphocytes are deceased as compared to the 25% (7 of the 28) mortality rate for crossmatch-negative patients. All four of the deceased patients with a positive crossmatch had demonstrable deposition of immunoglobulins in the capillaries of the donor heart at autopsy, whereas no immunoglobulin or fibrinogen deposition was seen in the hearts of crossmatch-negative patients. Three of the four patients with positive crossmatches had sera cytotoxic to lymphocytes of more than 25% of the persons, of a 42 member panel, whereas in the remaining one the serum was cytotoxic to less than 5% of the panel members. In crossmatch-negative patients, the sera were cytotoxic to less than 20% of the panel members with one exception. The relevance of cytotoxic antibodies to lymphocytes of panel members, crossmatch, and tissue deposition of immunoglobulins is discussed.

Adult↗
PubMed1983

B-lymphocyte typing (HLA-DR, MT, MB) interlaboratory reproducibility.

Frozen lymphocytes from six persons were shipped, in three batches, from one location to 11 other participating laboratories for B-lymphocyte typing. The cells were received by the participating laboratories in a frozen state with one exception. Four of the 12 (including the host laboratory) used two-color fluorescence method, seven used nylon wool to isolate B-lymphocytes followed by cytotoxicity testing using trypan blue, and one laboratory used goat anti-human F(ab')2 to isolate B-lymphocytes for two cells and two-color fluorescence method for the other four cells. Reference antigen assignments of the various cells were essentially the consensus of the various laboratories and major departures from those assignments were considered errors. Twelve typing errors were seen in a total of 67 typings (for HLA-DR), i.e., an error rate of 17.9%. Four of the errors might have been due to the poor state of cells resulting from the participating laboratory storing the cells for over four weeks before testing. Six of the 12 laboratories were in agreement for all six cells and made no errors. Typing for MT antigens did not appear to be particularly reliable, but there was a good interlaboratory correlation for MB antigen assignments. The exercise has established the feasibility of conducting a cell exchange for B-lymphocyte typing over a fairly wide geographic area, and has shown that the overall error rate for HLA-DR antigen assignments is about three times the rate of HLA-AB typing.

B-Lymphocytes↗
PubMed1983

[Comparative study of different fixation methods of 4-loop Binkhorst intraocular lens implants].

Intra- and postoperative complications of iris-fixated 4-loops Binkhorst intraocular lens implants were studied. The horizontally placed lens implants, iris-fixated with two prolene sutures, were found to be the best placed, considering complications like lens subluxation or dislocation. Iris angiography, done 9 days to 18 months after lens implantation, revealed no local iris damage at the suture sites.

Adult↗
PubMed1983

Comparative study of continuous and interrupted sutures in cataract surgery in eye camps.

In a comparative study performed to evaluate continuous and interrupted corneoscleral sutures under special conditions of eye operation camps, we performed 86 cataract extractions under local anaesthesia. Ab interno corneoscleral incisions were closed with 8/0 virgin silk suture material by three interrupted sutures in 42 cases and by five-bite continuous sutures in 44 cases. The results and complications were comparable in the two groups. Keeping in view the large number of cataract operations to be done under eye camp conditions, three interrupted sutures were found more advantageous than five-bite continuous sutures. These needed less time for placing and removal, caused less tissue damage and gave equally good results.

Cataract Extraction↗
PubMed1983

Pulmonary alveolar proteinosis. Staining for surfactant apoprotein in alveolar proteinosis and in conditions simulating it.

Formalin fixed paraffin-embedded lung tissues (n = 13) or lavage material (n = 4) from 17 patients in whom alveolar proteinosis was the primary disease and ten patients with other primary diagnoses but lung morphology similar to alveolar proteinosis were examined. The tissues were stained by the periodic acid-Schiff method and by the immunoperoxidase method for surfactant specific apoprotein. The intra-alveolar material in patients with primary alveolar proteinosis stained uniformly for surfactant specific apoprotein, whereas the staining was focal in patients with other primary diseases associated with intra-alveolar accumulation of proteinacious material. In both situations, the number of inflammatory cells, especially macrophages, was small. These observations extend an earlier impression about the presence of surfactant specific apoprotein in alveolar spaces in patients with primary alveolar proteinosis and provide a distinction between primary alveolar proteinosis and morphologically similar appearance due to other causes, ie, secondary alveolar proteinosis. The lack of macrophages in the affected tissue appears to be the major pathogenetic factor in alveolar proteinosis.

Apoproteins↗