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Biomedical subjects

G Sansone

Publications and source records attributed to G Sansone.

At least 73 records · Page 4Linked to original sources
PubMed1982

[The presence of 3,4-benzopyrene in plants I. Methods].

Purification methods of vegetable extracts have been experienced during our researches on the possible presence of the aromatic polycyclic hydrocarbons (particularly 3,4 benzopyrene) in vegetables. The validity of different methods has been determined by IR spectrophotometric analysis using special apparatus (IR-DATA-STATION). Vegetables extracts have been purified efficaciously by column chromatography, saponification and special methods (Grimmer's method). The described method eliminates, nearly entirely, the polluting substances that could alternate the chemical analysis of the vegetable extracts.

Benzo(a)pyrene↗
PubMed1982

Decreased glycosylation of band 3 and band 4.5 glycoproteins of erythrocyte membrane in congenital dyserythropoietic anaemia type II.

We report a study of HEMPAS erythrocyte membrane glycoproteins in relation to proteolytic digestion and surface labelling with galactose-oxidase/NaB[3H]4. The proteolytic digestion of band 3, the major intrinsic glycoprotein of the human erythrocyte membrane, reveals an abnormality in the outer glycosylated segment of this protein. 3H incorporation in band 3 and band 4.5 glycoproteins after treatment with galactose-oxidase/NaB[3H]4 is reduced in HEMPAS red cells suggesting a defective glycosylation of these proteins. These findings together with the persistence of i antigen and the normal presence of I antigen lead us to conclude that erythroblastic membrane features may persist in HEMPAS erythrocytes.

Adult↗
PubMed1982

Hb Belfast (beta 15 Trp replaced by Arg) in an Italian family.

An uncommon abnormal hemoglobin, Hb Belfast (beta 15 Trp replaced by Arg) was discovered in a patient of Italian origin. The patient was a 42-year-old man who developed two episodes of jaundice after a prolonged administration of an antituberculous drugs. Family studies revealed that several members were asymptomatic carriers of Hb Belfast.

Adult↗
PubMed1981

[Action of the unsaponifiable components of the most common edible oils on the growth of the alga Euglena gracilis. Preliminary studies].

Within the sphere of the researches the biological effects of the most common edible oils (peanuts, sunflower, maize, soya and rectified olive) it has been studied the interaction between the development of the Euglena gracilis unicellular seaweed and the presence of the unsaponifiables examined in their cultures. As a biologically active substance it has been used 3,4-benzopyrene. Spectrophotometric analysis have evidenced that all the unsaponifiables, especially those of the soya seeds, caused a growth decrease of the seaweed culture. A similar effect is found in the cultures treated with aromatic hydrocarbon.

Animals↗
PubMed1981

A nucleotide change at a splice junction in the human beta-globin gene is associated with beta 0-thalassemia.

beta 0-Thalassemia is a heterogeneous group of disorders associated with absence of beta-globin. In a survey of DNAs from patients with beta 0-thalassemia of diverse ethnic origins, a change at the splice junction at the 5' end of the large intervening sequence (IVS 2) of the human beta-globin gene has been found in one patient of Italian and another two of Iranian ethnic origins. The enzyme Hph I recognizes a change at this site and generates a large-than-normal fragment of DNA, which hybridizes specifically to a beta-globin IVS 2 probe. No other changes in beta-globin gene DNA structure or organization are detectable by extensive restriction endonuclease analysis. The enzyme HinfI which recognizes a sequence beginning three nucleotides from the 5' end of the IVS 2 splice junction, produces normal fragments and localizes the defect to a G-G-T sequence at the 5'-end IVS 2 splice junction. This sequence is known to be remarkably conserved in all globin genes from many species and in most other genes examined to date. Thus, in at least some beta 0-thalassemia patients, the beta 0-thalassemia defect is associated with a nucleotide change at a splice junction. These patients provide unique examples of naturally occurring defects in splice junctions of eukaryotic genes associated with absence of specific gene function.

Base Sequence↗
PubMed1981

New genetic variants of glucose 6-phosphate dehydrogenase (G6PD) in Italy.

Six new variants of human erythrocyte G6PD have been characterized. All of them were found in Italian males and all were associated with enzyme deficiency, but only two with signs of haemolysis. These and other variants reported in the literature, which must thus far be regarded as sporadic, are found to map in parts of Italy where common types of G6PD deficiency are also prevalent.

Adolescent↗
PubMed1980

[In vitro action of unsaponifiable edible oils on calf renal cell cultures].

During the research concerning biological effects of the most common edible oils (peanuts, sunflower, maize, soya and rectified olive), the Authors have tested the insaponificable fractions on the calf kidney cellular monosurface. The insaponificable of the maize seeds oil determine a net decrease of the speed growth. It has also been noted a very slight increase of the cells development, in the cultures treated with the insaponificables of some other oils, compared with the samples of those not treated. As comparison substance it has been used 3,4 benzpyrene, because his toxic action on the cells is well known.

Animals↗
PubMed1980

[Effect of the unsaponifiables of the most common edible oils on the development of E. coli bacteria].

The writers within the context of the research on the biological actions of the unsaponificables in the most common edible oils, have tested the effects produced by such unsaponificables on the culture of E. Coli. The unsaponificables, and those of seed oils of peanuts and sunflowers in particular, aid the doubling of the E. Coli. Similar research on the culture of animal cells (in vitro) and unicellular algae, are at present outstanding.

Arachis↗
PubMed1980

[Biological effects of non-saponifiable sunflower seed oil. Preliminary research].

By inserting near the under-axillary gland of the rats Wistar (one month age) compressed material containing g. 0.250 of non-saponificable sunflower seeds oil, it has been noted an increase of the number of the mitosis in the under-axillary gland, in the lung and some mitosis in the liver. Such results are similar to those obtainable by administering, with similar method 0.050 g. of 3,4benzopyrene or 0.100 g. of the hydrocarbon oxidization products. The histological analysis have been effected on the organs of rats sacrificed one month after inoculation.

Animals↗
PubMed1980

[Localization and individualization of abnormal carbonates in the valve of Mytilus galloprovincialis].

The cristallographic analysis of the valve of Mytilus galloprovincialis using the scansion microscopy method shows probable inclusions of Cd and Zn in the carbonate calcite layer and probable inclusions of Pb in carbonate aragonite layer. This confirms the supposition of a substitution of Ca ions from heavy metals investigated and the formation of the respective carbonates that precipitate together with calcium carbonate in the process of growth of the valve of the mollusc examined.

Animals↗
PubMed1980

The first stable variant of erythrocyte glucose-phosphate isomerase associated with severe hemolytic anemia.

A new variant of glucosephosphate isomerase (GPI) associated with hemolytic anemia, mental retardation, and muscular hypotonia is described. The defective enzyme showed increased affinity for fructose-6-phosphate (F-6-P), decreased affinity for glucose-6-phosphate (G-6-P) altered electrophoretic and isoelectrofocusing patterns, and shift to the left of the precipitin curve. The enzyme was stable under all the conditions tested (heat, urea, guanidine-HCl, and storage). Optimum pH, Ki for 6-phosphogluconic acid (6-PGA) and for erythrose-4-phosphate (E-4-P), molecular weight, GPI-related antigen concentration, immunodiffusion pattern, and immunoinactivation were in the normal range. This is the first example of the association of a stable mutant GPI with severe hemolytic anemia. Enzyme instability has been present in all previously reported cases.

Anemia, Hemolytic, Congenital↗
PubMed1979

[Interaction between alkaloids of Claviceps purpurea and development of some bacteria and bacteriophages. Preliminary experiments].

It has been tested the bacteriolytic activity and the interaction among the development of the bacteriophage T4 and Ergot alkaloids. It has been determined a bacteriolytic action on the bacterial stub "E. Coli host of bacteriophage T4. It exist an interaction also among such alkaloids and the development of the bacteriophage T4, which appears through an increase of the quantity of lysis areas, becoming evident in solid bodies containing the lysing bacterium. Further researches with other bacterial and virus stubs will be effected and mentioned afterward.

Alkaloids↗
PubMed1979

[Anionic detergents in Mytilus Galloprovincialis of the gulf of Naples].

The authors have investigated on the anionics detergents rate inside the Mytilus Galloprovincialis, used as animal-monitor of the environment state of a certain part of the Gulf of Naples. The data concerning 14 zones in which were drown mussels cultivated in the Gulf of Naples resumed in a specific list, shown an high concentration of above detergents.

Animals↗