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G Sansone

Publications and source records attributed to G Sansone.

At least 55 records · Page 3Linked to original sources
PubMed1989

Interaction of proteins RSV IV and RSV V in rat seminal vesicle secretion.

The RSV IV polypeptide, molecular weight ratio (Mr = 10,000), which is produced by the rat seminal vesicle, has previously been suggested to be associated with another polypeptide in the gland secretion (Higgins et al., '76). This study provides that RSV IV is a component of a protein shown by immunoassays, electrophoresis, and amino acid composition analysis to contain, together with RSV IV, the seminal vesicle secretory RSV V polypeptide (Mr = 13,000). This RSV IV-RSV V complex (namely CFS protein) had an isoelectric point at pH 7.2 and an approximate molecular weight of 22,000 daltons. This complex inhibits the previously reported in vitro binding of the isolated RSV IV to epididymal sperm cells, thus suggesting a functional role for the RSV IV-RSV V interaction.

Amino Acids↗
PubMed1988

Detection of sperm-coating antigens immunologically related to a seminal protein in rat.

We report in this paper that proteins from the surface of ejaculated spermatozoa contain antigenic determinants cross-reacting with a rabbit antiserum raised against native CFS, a protein secreted from the rat seminal vesicle and composed of two subunits, namely RSV IV and RSV V. Conversely, no such proteins could be extracted from cauda epididymal spermatozoa. The cross-reacting proteins derived from the ejaculated spermatozoa were analyzed by SDS-PAGE. An electrophoretic pattern different than that expected for native CFS in denaturing conditions was found. In vitro reconstitution experiments showed that labeled native CFS is able to bind cauda epididymal spermatozoa. The CFS protein recovered from the sperm surface was examined and alterations of its structure were also noted. The sperm-coating abilities of CFS and of its RSV IV subunit are discussed.

Animals↗
PubMed1986

Congenital dyserythropoietic anemia type I: report of a pair of siblings.

Two siblings affected with congenital dyserythropoietic anemia type I are described. They are the sixth familial occurrence reported. Particularly interesting is the comparison between the course and laboratory data of our cases. An unusual finding is the presence of the antigen 'i' on the erythrocytes of both patients.

Anemia, Dyserythropoietic, Congenital↗
PubMed1984

[Selective incorporation of metals in aragonite and calcite into the shell of Mytilus galloprovincialis in relation to the ionic radius].

The Authors assuming as hypothesis that elements beyond Calcium could be included as carbonate according to crystalline structure of Aragonite and Calcite in the shell of Mytilus g. have verified the selective inclusion of Cr, Mg, Zn, Fe, Cu, Cd, Pb in the biocrystals in relation to their ionic radius. Though the ratio of concentrations of these elements in the shell layers: [Me] calcite/[Me] aragonite ( [C]/[A] ) is significatively greater than 1 for Zn and Mg, significatively less than 1 for Cr and Pb and the no-significative distribution of Cu, Fe, Cd do not allow a sure relationship between the ionic radii of Ca and the investigated elements in the process of shell inclusions. However, authors, do not exclude that these elements may be included differently from the relative aragonitic or calcitic crystalline carbonate structures.

Animals↗
PubMed1984

[Influence of the accumulation of Cr, Cd and Pb on the pool of free tissue amino acids in Mytilus galloprovincialis in various zones of the bay of Naples].

The Authors investigated on the relations between the amount of Heavy Metals (Me) and free aminoacids (FAA) from tissues of Mytilus galloprovincialis. Similar mussels were collected from three sampling zones of bay of Naples. The analyzed Me were Cr+3, Cd+2, Pb+2. The obtained results show that behaviour of Me and FAA is similar. These data suggested to the Authors that FAA changes have to be related to an attempt to recover their's equilibrium more than a direct influence of Me on mussel's FAA metabolism.

Amino Acids↗
PubMed1984

Acute hemolytic anemia induced by a pyrazolonic drug in a child with glucose-6-phosphate dehydrogenase deficiency.

In a 36-month-old child a severe hemolytic anemia occurred after the administration of a pyrazolonic drug. Erythrocyte glucose-6-phosphate dehydrogenase activity was absent in the patient. Intermediate enzymatic levels were demonstrated in the mother and in the maternal grand-mother with a corresponding erythrocyte mosaicism at the methemoglobin elution test. Some cells showed Heinz bodies and peculiar changes in the morphology in the initial phase of the crisis. There is no mention in the literature of a hemolytic action of this drug.

Aminopyrine↗
PubMed1984

Congenital dyserythropoietic anaemia type II associated with a new type of G6PD deficiency (G6PD Gabrovizza).

A 6-year-old boy with chronic haemolytic anaemia was found to have glucose 6-phosphate dehydrogenase (G6PD) deficiency and the morphological, ultrastructural and serological features of congenital dyserythropoietic anaemia (CDA) type II. The patient's mother was heterozygous for G6PD deficiency. G6PD from the patient's red cells, upon partial purification and full characterization, was found to be a new variant designated G6PD Gabrovizza. We conclude that two distinct genetic abnormalities coexisted in this patient. We suggest that CDA type II may become clinically more expressed when another abnormality of the erythrocytes coexists.

Anemia, Dyserythropoietic, Congenital↗
PubMed1984

The characterization of hemoglobin Manitoba or alpha (2)102(G9)Ser----Arg beta 2 and hemoglobin Contaldo or alpha (2)103(G10)His----Arg beta 2 by high performance liquid chromatography.

Hb Contaldo with a His----Arg substitution at position 103(G10) of the alpha chain is a newly discovered unstable Hb variant observed in an Italian child. Its instability is probably due to the disruption of the hydrogen bond between alpha 103(G10)His and beta 108(G10)Asn. The structural variation in the core segment was determined through analysis of tryptic peptides from digests of the alpha X and oxidized alpha X (with performic acid) chains, which were separated by HPLC. Similar analyses were made for the alpha X chain of the rare Hb Manitoba in which alpha 102(G9) Ser is replaced by Arg. This variant was observed for the first time in an Italian patient, and was also studied in a member of a previously described Canadian family.

Amino Acids↗
PubMed1984

Alternate organization of alpha G-Philadelphia globin genes among U.S. black and Italian Caucasian heterozygotes.

Seven Hb G-Philadelphia (Hb G) heterozygotes from three Caucasian families from Northern Italy and Sardegna were found to have proportions of Hb G averaging 23%. This value is considerably lower than the 34% or 48% found in Blacks from the Southeastern U.S.A. in whom the alpha G gene is in linkage with alpha-thalassemia-2, i.e. the alpha o alpha G/alpha alpha or alpha o alpha G/alpha o alpha genotypes. Gene mapping identified tandem organization of the alpha G gene in cis with a normal alpha A gene, i.e. the alpha alpha G/alpha alpha genotype, among the Hb G heterozygotes from Italy. The data on the Italian heterozygotes are similar to those obtained by Bruzdzinski et al (14) on a Black family. These results indicate alternate organization of the alpha G genes probably across racial or ethnic boundaries. Comparison of the mean cellular globin amount of alpha G/alpha G gene/cell among Hb G heterozygotes with 4, 3, 2 or 1 alpha globin genes (i.e. alpha A + alpha G) revealed considerable reactivation of individual alpha genes in conditions of mild to severe alpha globin deficiencies.

Adolescent↗
PubMed1983

[Different shell development in Mytilus galloprovincialis in 2 different populations from the Gulf of Naples].

The size frequency distribution, the shell weight, the semilogarythmic growth, the carbonic anhydrase activity has been investigated between two populations of mussels from different sampling zones. Significative differences were found in the parameters investigated between the two populations. The authors conclude that the reason of this morphological and biochemical differences may arise from different natural hydrobiological factors and/or from different pollution of their habitat.

Animals↗
PubMed1983

[Organic matrix in biocrystals in the Mytilus galloprovincialis shell. Scanning microscopy].

The Authors have investigated the structural property of organic shell matrix from Mytilus galloprovincialis by scanning microscopy. The microscopic investigation shows differences between matrix from nacreous layer or argonite and matrix from outer layer or calcite. The first shows a "cavernous" surface; the other instead shows a "smooth" surface. The Authors conclude that probably these differences may influence the different crystallographic arrangement of biocrystals.

Animals↗
PubMed1983

[Accumulation of heavy metals in biocrystals in the shell of Mytilus galloprovincialis from different zones of the Gulf of Naples].

The amounts of Fe, Zn, Cu, Mg were determined in the inner and outer layers of shells of Mytilus g.. Different values significatively between the layers were found only for Mg. The ratio [Me]Calcite/ [Me]Aragonite was calculated and it is not more than one, for all the colonies of different sampling zones. The Authors conclude that perhaps the reason of this behaviour is the different pollution of their habitat.

Animals↗
PubMed1983

The occurrence of different levels of G gamma chain and of the A gamma T variant of fetal hemoglobin in newborn babies from several countries.

The gamma chain compositions of the fetal hemoglobins of 2453 newborn babies from East Asian countries (1350 babies), from Italy, Yugoslavia, Bulgaria, and Georgia (417 Caucasian babies), and 686 black babies from Georgia were determined by high pressure liquid chromatography. Unusual results for a limited number of babies were confirmed by chemical analyses, and were evaluated further by family studies. Statistical analyses indicated high gene frequencies for the A gamma T chain in Italian (f = 0.237), Yugoslavian and Bulgarian (f = 0.238), and white Georgia babies (f = 0.224), a lower frequency in Japan (f = 0.178), and India (f = 0.173), and particularly in mainland China (f = 0.079). The A gamma T gene frequency in normal (AA) Black babies was 0.102. When a beta S or beta C mutation was also present this frequency was greatly decreased, particularly in babies with the AC condition (f = 0.036). These results suggest the near absence of the A gamma T mutation on the chromosome also carrying the beta C determinant. Most babies had the expected G gamma values which vary between 60 and 80%, but several (mainly black) babies had higher values (between 80 and 90%), while one normal black baby had a G gamma value of (nearly) 100%. This condition may be a form of A gamma +1-thalassemia and has been discussed in detail elsewhere (Blood 58:491-500, 1981). Thirty-five clinically normal (mainly Chinese, Indian, and Japanese) babies had G gamma values of about 40%. Twenty-six babies had A gamma I values of about 60%, while the remaining nine babies had A gamma T and A gamma I chains in a ratio of either 1 to 2 or 1 to 1. Two additional newborns did not produce any G gamma chains, but had only A gamma I chains or A gamma T chains. Family studies failed to indicate a specific hematological abnormality. These unusual ratios between the G gamma and A gamma (either A gamma I or A gamma T) chains have led to speculations regarding possible genetic abnormalities present in these infants.

China↗