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Biomedical subjects

G Rossi

Publications and source records attributed to G Rossi.

At least 775 records · Page 43Linked to original sources
PubMed1978

Metabolism and biological activity of a thiamine new salt. I. Urinary excretion and tissue content of vitamin B1 in rats treated with thiamine chloride ester monophosphate of Bis-glucosamine.

Research has been carried out on the metabolism of thiamine chloride ester monophosphate of bis-D-glucosamine (TCMPG) evaluating the urinary excretion, the blood and the hepatic content of thiamine in the rat after intraperitoneal injection of this compound. The results obtained have shown a greater retention of vitamin B1 when it is administered as glucosamine salt. In fact, the amount of thiamine excreted by the rats which have received the TCMPG is lower while the blood and liver levels of vitamin are higher, compared with the control rats treated with thiamine monophosphate only, or with thiamine and glucosamine. These results permit the conclusion that glucosamine increases the utilization of the vitamin and its penetration into the cells.

Animals↗
PubMed1978

[Effect of acoustic trauma on the ganglion of Corti].

Ultrastructural characteristics of cells in Corti's ganglion have been studied in guinea pigs subjected to acoustic stress of white wide-stripped noise (100 db for 12 h a day, for 30 days). The earliest regressive signs appear in the cells at the level of the 2d helix. They demonstrate rejection of plasmic membrane from the myelin sheath and cytoplasmic compression resulting in destruction of the whole cell. In lemmocytes, in ganglion cells and in their sheaths sharply osmiophilic myelin and multivesicular bodies are often seen. Destruction of myelinated neurons has been described. Regressive changes have not been revealed in unmyelinated neurons, the latter composing about 2% of all ganglion cells.

Animals↗
PubMed1978

Studies on metabolism of thiamine chloride ester monophosphate of bis-D-glucosamine. II. Effect of TCMPG in vitamin B1-deficient rats.

In order to investigate the metabolism of thiamine chloride ester monophosphate of bis-D-glucosamine (TCMPG) urinary excretion, blood levels and tissue content of thiamine in vitamin B1-deficient rats were measured after intraperitoneal injection of this compound or of thiamine monophosphate or of a mixture of thiamine monophosphate and glucosamine. The greater retention and the higher levels of vitamin observed in the blood and in the tissues of animals treated with TCMPG compared with the control rats confirm the hypothesis that a more efficient utilization of vitamin B1 is obtained by the organism when it is administered salified with glucosamine.

Animals↗
PubMed1978

BUdR as a tracer of the possible mutagenic activity of Pb++ in human lymphocyte cultures.

The method of SCE was used as an indicator of the possible mutagenic activity of lead acetate in in vitro cultures of human lymphocytes. After determining the optimal concentration of BUdR, the cultures were treated with increasing doses of the metal. The negative results obtained with this method led us to examine other parameters, in particular the classical cytogenetic aberrations.

Bromodeoxyuridine↗
PubMed1977

[Use of deoxyribonuclease in the therapy of the neoplastic patient].

The anti-inflammatory actvity of desoxyribonuclease has been demonstrated on a number of occasions. It has been suggested that its particular features may mean that it possesses an antineoplastic action, especially if employed in asociation with radiation or chemotherapy. The drug, which depolymerises DNA, was administered for 15-60 days [ 1 million U. on alternate days] over a period of 1 yr. in a series of 99 patients, with or without radiation or chemotherapy. No evidence of its antineoplastic activity was obtained, though the anti-inflammatory effect was distinctly observed. Quickly reversible signs of intolerance were noted in about 4% of the series.

Antineoplastic Agents↗
PubMed1977

Assay and partial characterization of the solubilized cell surface receptor for immunoglobulin E.

The cell surface component (receptor) which specifically binds immunoglobulin E (IgE) presumably forms an integral part of the functional chain involved in the antigen-induced IgE-mediated degranulation of histamine-containing mast cells and basophils. This paper describes a simple (NH4)2SO4 predipitation assay with which the interaction of IgE with detergent-solubilized receptors can be reproducibly quantitated. Receptor saturation was demonstrated and a linear response to receptor concentration over at least a 30-fold rang obtained. By means of the assay it was shown that (a) all assayable receptors of rat basophil leukemia cells are cell surface expressed; (b) receptor specificity remains intact during solubilization; (c) the binding constants of the solubilized IgE receptors are similar to those determined on intact cells. Utilizing agarose gel filtration, preliminary estimates of the molecular weight of the active free solubilized receptor and of its complex with IgE suggest that the receptor is univalent.

Ammonium Sulfate↗
PubMed1977

Molecular weight and valence of the cell-surface receptor for immunoglobulin E.

The molecular weight of the active solubilized cell-surface receptor for immunoglobulin E (IgE) was measured in nonionic detergent. The diffusion coefficient was estimated by gel filtration, the partial specific volume was estimated from the differential sedimentation in sucrose gradients prepared from H2O and D2O, and the sedimentation constant was estimated from the same centrifugation experiments. The receptor has an apparent molecular weight of 130,000. Its high partial specific volume (0.81 cm3/g) suggests that bound detergent contributes significantly to the mass. The molecular weights of the receptor-IgE complex and of unbound IgE determined similarly were 310,000 and 200,000 respectively, clearly showing that the receptor is univalent. The implications of these results for the subunit structure of the receptor, receptor-membrane integration, and a possible mechanism of receptor triggering are discussed.

Animals↗
PubMed1977

Intraoperative antibiotic wound lavage: an attempt to eliminate postoperative infection in arterial and clean general surgical procedures.

Intraoperative antibiotic wound lavage has been used in all arterial reconstructive procedures for the past 7 years and reduced the incidence of early and late postoperative infections from 1.5% in 400 operations prior to 1969 to 0.23% (one post-hospital) in 434 patients operated since that date. In 226 consecutive clean major general surgical procedures since June 1971 there has been no early or late wound infections following intraoperative antibiotic wound lavage in contrast to an infection rate of 1.5% in 185 operations prior to that date. A double blind study of 200 patients undergoing operations for varicose veins was carried out as follows: The wounds of alternate patients were irrigated either with normal saline or with antibiotic solution. There were no gross (grade II) postoperative wound infections. Minor skin changes were noted in 93 of 632 incisions in the saline group and only 49 of 608 incisions irrigated with antibiotic solution (P less than 0.001). In clean operations without antibiotic wound lavage there was a 0.73% rate of in-hospital wound infections in 685 patients in contrast to a zero rate in 760 patients wherein intraoperative wound lavage was carried out throughout the operative procedure.

Aged↗
PubMed1977

Automatic audiometry in stapes motility evaluation.

An automatic Grason-Stadler-Békésy 1701 audiometer with continuous frequency progression (1 octave/min) and increase (or attenuation)of intensity of 2.5 dB/sec, has been employed to perfect an investigation technique for obtaining, by the mean difference in dB between absolute bone conduction (ABC) and relative bone conduction(RBC), a quantity evaluation of stapes motility and monitor alterations brought on by various disease processes. A preliminary study performed on subjects with normal hearing was used to establish the influence of age on motility. It was thus possible to establish an index (stapes motility index, SMI) to represent the degree of normal stapes motility as a function of the subject's age and thus, through variations in this index, identify alterations to the motility of the tympano-ossicular system caused by ongoing disease processes and by surgery.

Adult↗
PubMed1976

Nonanastomotic aneurysms in venous homologous grafts and bovine heterografts in femoropopliteal bypasses.

In the absence of a suitable autologous vein for a long distal femoropopliteal bypass, the homologous vein appears to be a good substitute. However, the patency rate after one year is less than 50 per cent and nonanastomotic aneurysms occurred in one third of our patients followed for more than one year. These two complications may be related to an immune response which may be lessened by preservation of the graft in the frozen state. Bovine heterografts function well when placed subfascially for femoropopliteal bypasses ending above the knee. Failure rate is high when the distal end of the graft extends across the knee joint. In our experience nonanastomotic aneurysms have developed in 50 per cent of patent grafts followed for more than one year, although other surgeons have reported an incidence of only 3 to 20 per cent. In the follow-up of twenty-three venous homografts and twenty bovine heterografts placed as femoropopliteal bypasses, eight nonanastomotic aneurysms appeared and required replacement. This occurrence was noted in more than 33 per cent of grafts patent beyond one year. When an adequate autologous vein is not available, the composite Dacron-vein graft is the best substitute.

Aged↗
PubMed1976

Isolation of antibody-forming cells by using cluster formation in combination with velocity sedimentation.

A method is described for isolation of antibody-forming cells with the help of cluster formation. This was achieved by incubating spleen cells from immunized mice with sheep erythrocytes at 37 degrees C. During incubation rabbit-anti-mouse immunoglobulin serum was present in order to facilitate the formation of clusters. Thereafter the clusters were isolated by velocity sedimentation. In this way we obtained suspensions containing more than 80% clusters. Some of the cells in the clusters still produced antibodies against sheep erythrocytes cultured for two days in vitro.

Animals↗
PubMed1976

Anxiety therapy in the neoplastic patient.

A clinical study was carried out in 73 neoplastic patients suffering from anxiety and other emotional upsets to assess the effectiveness and tolerance of lorazepam. Patients were given individualised daily doses ranging from 1.5 mg to 3 mg lorazepam for 15 to 60 days. Results, as assessed by the response of anxiety, tension, erethism and insomnia, showed that only 4 (5%) patients failed to obtain some relief. There was complete disappearance of all symptoms in 29 (40%) after 15 days, relief of at least one major symptom and reduction in the others in 27 (37%), and slight reduction in one or more symptoms in 13 (18%) patients. Side-effects were minimal and disappeared within a few days with continued treatment.

Adult↗